Measuring spatiotemporal ultrafast field structures of pulses from multimode optical fibers.

Ultrashort pulses emerging from multimode optical fibers are spatiotemporally complex, because inside these fibers the modes have different spatial intensity patterns and experience different propagation velocities and dispersions. To determine the spatiotemporal field from multimode fibers, we applied a technique for the complete measurement of the output pulses called a spatially and temporally resolved intensity and phase evaluation device: full information from a single hologram. It yields the complete electric field over space and time from multiple digital holograms, simultaneously recorded at different frequencies on a single camera frame. Using femtosecond pulses from a Ti:sapphire laser, we measured the first few linearly polarized modes (LP01, LP11, LP02, and LP21) inside several few-mode fibers. We also generate movies displaying the measured spatial, temporal, and spectral field features.

Visualizing spatiotemporal pulse propagation: first-order spatiotemporal couplings in laser pulses.

Even though a general theory of first-order spatiotemporal couplings exists in the literature, it is often difficult to visualize how these distortions affect laser pulses. In particular, it is difficult to show the spatiotemporal phase of pulses in a meaningful way. Here, we propose a general solution to plotting the electric fields of pulses in three-dimensional space that intuitively shows the effects of spatiotemporal phases. The temporal phase information is color-coded using spectrograms and color response functions, and the beam is propagated to show the spatial phase evolution. Using this plotting technique, we generate two- and three-dimensional images and movies that show the effects of spatiotemporal couplings.

Numerical simulations of holographic spatiospectral traces of spatiotemporally distorted ultrashort laser pulses.

We simulate traces for a catalog of spatiotemporally complex pulses measured using a single-shot complete spatiotemporal pulse-measurement technique we recently developed, called Spatially and Temporally Resolved Intensity and Phase Evaluation Device: Full Information from a Single Hologram (STRIPED FISH). The only such technique ever developed to our knowledge, STRIPED FISH measures the complete spatiotemporal intensity I(x,y,t) and phase ϕ(x,y,t) of an arbitrary laser pulse using an experimentally recorded trace consisting of multiple digital holograms, one for each frequency present in the pulse. To understand the effects of various spatiotemporal distortions on the STRIPED FISH trace, we numerically investigate STRIPED FISH trace features for a catalog of pulses, including the spatially and temporally transform-limited pulse, temporal and spatial double pulses, spherically focusing and diverging pulses, self-phase modulated and self-focusing pulses, spatiotemporally coupled pulses, and pulses with complex structures. As a practical example, we also analyze an experimentally recorded trace of a focusing pulse with spatial chirp. Overall, we find that, from STRIPED FISH's informative trace, significant spatiotemporal characteristics of the unknown pulse can be immediately recognized from the camera frame. This, coupled with its simple pulse-retrieval algorithm, makes STRIPED FISH an excellent technique for measuring and monitoring ultrafast laser sources.

Standards for ultrashort-laser-pulse-measurement techniques and their consideration for self-referenced spectral interferometry.

Issues important for new ultrashort-pulse-measurement techniques include the generation of theoretical example traces for common pulses, validity ranges, ambiguities, coherent artifacts, device calibration sensitivity, iterative retrieval convergence, and feedback regarding measurement accuracy. Unfortunately, in the past, such issues have gone unconsidered, yielding long histories of unsatisfactory measurements. We review these issues here in the hope that future proposers of new techniques will consider them without delay, and, as an example, we address them for a relatively new technique: self-referenced spectral interferometry.

A randomized trial of internet-based versus traditional sexual counseling for couples after localized prostate cancer treatment.

BACKGROUND: After treatment for prostate cancer, multidisciplinary sexual rehabilitation involving couples appears more promising than traditional urologic treatment for erectile dysfunction (ED). The authors of this report conducted a randomized trial comparing traditional or internet-based sexual counseling with waitlist (WL) control. METHODS: Couples were randomized adaptively to a 3-month WL, a 3-session face-to-face format (FF), or an internet-based format (WEB1). A second internet-based group (WEB2) was added to examine the relation between web site use and outcomes. At baseline, post-WL, post-treatment, and 6-month, and 12-month follow-up assessments, participants completed the International Index of Erectile Function (IIEF), the Female Sexual Function Index (FSFI), the Brief Symptom Inventory-18 to measure emotional distress, and the abbreviated Dyadic Adjustment Scale. RESULTS: Outcomes did not change during the WL period. Of 115 couples that were randomized to FF or WEB1 and 71 couples in the WEB2 group, 34% dropped out. Neither drop-outs nor improvements in outcomes differed significantly between the 3 treatment groups. In a linear mixed-model analysis that included all participants, mean ± standard deviation IIEF scores improved significantly across time (baseline, 29.7 ± 17.9; 12 months, 36.2 ± 22.4; P < .001). FSFI scores also improved significantly (baseline, 15.4 ± 8.5; 12 months, 18.2 ± 10.7; P = .034). Better IIEF scores were associated with finding an effective medical treatment for ED and normal female sexual function at baseline. In the WEB2 group, IIEF scores improved significantly more in men who completed >75% of the intervention. CONCLUSIONS: An internet-based sexual counseling program for couples was as effective as a brief, traditional sex therapy format in producing enduring improvements in sexual outcomes after prostate cancer.

Psychosocial concerns of young African American breast cancer survivors.

Thirty-three African American breast cancer survivors age 45 or younger participated in semistructured phone interviews about psychosocial concerns. Twenty-six percent believed treatment interfered with employment. One third wanted additional emotional support at and after diagnosis. One half felt cancer negatively influenced romantic relationships. Forty-five percent wanted children at diagnosis, but one half these women never received fertility information. One third reported sexual problems, but 73% never discussed sexuality with providers. Fifty-two percent lacked information about cancer-related sexual dysfunction. The strength and spirituality of African American women may facilitate cancer adjustment, but cultural taboos surrounding women's health issues may interfere with successful coping.

Sisters Peer Counseling in Reproductive Issues After Treatment (SPIRIT): a peer counseling program to improve reproductive health among African American breast cancer survivors.

BACKGROUND: African American breast cancer survivors may be at high risk for reproductive health problems, including menopause symptoms, sexual dysfunction, and distress about cancer-related infertility. The authors partnered with Sisters Network Inc. to create the Sisters Peer Counseling in Reproductive Issues After Treatment (SPIRIT) program, a culturally sensitive intervention program that combined a written workbook and peer counseling. METHODS: Three hundred women were randomized to receive either the workbook plus 3 in-person sessions with a trained peer counselor or the workbook plus ≤ 30 minutes of telephone counseling to be initiated by the participant. Questionnaires at baseline, post-treatment, and at 6-month and 12-month follow-up assessed emotional distress, sexual function, relationship satisfaction, spirituality, menopause symptoms, and knowledge. Satisfaction with the program and the use of medical care also were assessed. RESULTS: Both groups of women improved significantly in knowledge, decreased in distress, and had decreased hot flashes. Sexually active women had improved sexual function at 6-month follow-up but not at 1 year. However, peer counseling had little incremental benefit over the telephone counseling. CONCLUSIONS: The SPIRIT program was rated very useful by 66% of women. Outcomes justify continued use of the workbook and further research to optimize the impact of peer counseling.

Expression and properties of recombinant ovine uterine serpin.

Ovine uterine serpin (OvUS) is produced in the uterus of sheep under the influence of progesterone. It weakly inhibits pepsin and reduces proliferation of lymphocytes, tumor cell lines, and preimplantation embryos. When purified from uterine fluid, the concentration required for its antiproliferative effect in vitro is approximately 0.25-1 mg/ml. Here we show that recombinant (r) OvUS is a more potent regulator of cell proliferation than native (n) OvUS purified from uterine fluid. To produce rOvUS, RNA was extracted from endometrium from a pregnant ewe and cDNA was amplified by reverse transcription-polymerase chain reaction using gene-specific primers. The purified OvUS cDNA was inserted into the ampicillin-resistant plasmid vector pcDNA3.1/ V5-His-TOPO. The plasmid was introduced into the TOP10 Escherichia coli strain, purified, and used for transfection of Freestyle 293-F cells. Digestion of rOvUS with protein N-glycosidase F confirmed that rOvUS was N-glycosylated. Both rOvUS and nOvUS inhibited proliferation of phytohemagglutin-activated sheep lymphocytes and the P388D1 mouse lymphoma and PC-3 prostate cell lines. Inhibition was greater for rOvUS than for nOvUS, and concentrations as low as 15 microg/ml rOvUS were effective at reducing lymphocyte proliferation. Addition of rOvUS to fertilized bovine embryos reduced the cleavage rate and the percentage of embryos that became blastocysts. Native OvUS did not affect cleavage rate and had a smaller effect on development to the blastocyst stage. Experiments demonstrate that OvUS is a more potent inhibitor of cell proliferation and embryonic development than previously believed and add credence to the putative role for the protein in regulating cell proliferation.

Litter characteristics of gilts artificially inseminated with transforming growth factor-beta.

PROBLEM: Semen is a rich source of transforming growth factor-beta (TGF-beta) and it has been proposed that this molecule promotes embryonic survival by modifying immune responses to promote tolerance toward paternal antigens and by inducing release of cytokines that promote embryonic development. The role of TGF-beta was tested using pigs by evaluating whether its addition to washed sperm improves conceptus survival and fetal growth. METHODS OF STUDY: At estrus, gilts were artificially inseminated twice at 12-hr intervals with 100 mL of either washed semen resuspended in a commercial semen extender supplemented with 2 mg/mL of gelatin or washed semen in the same extender containing 65 ng/mL of TGF-beta1. Three boars were used as semen donors. At day 80 (+/-4 days) of gestation, gilts were sacrificed and reproductive tracts harvested. RESULTS: Treatment had no effect (P > 0.10) on total or live fetuses per litter, implantation rate, fetal survival or percentage of corpora lutea resulting in live fetuses at day 80. Insemination with TGF-beta1 also did not affect total or average fetal weight or total placental weight. There was a tendency (P = 0.09) for average placental weight of live fetuses to be lower for pregnancies established in gilts treated with TGF-beta1. Also, placental efficiency (mass of fetus/mass of placenta) was greater (P < 0.05) for pregnancies established in gilts treated with TGF-beta1. The high fertility in control gilts (80% implantation rate and 11.5 live fetuses per litter) is indicative that soluble seminal factors are not necessary for the establishment of pregnancy. CONCLUSIONS: Within the ranges tested, concentration of TGF-beta in the fluid phase of the inseminate is not an important determinant of conceptus survival or fetal and placental growth to day 80 of gestation in the pig.

Ionic reaction of halogens with terminal alkenes: the effect of electron-withdrawing fluorine substituents on the bonding of halonium ions.

Ionic reactions of terminal alkenes with chlorine (Cl(2)), bromine (Br(2)), and iodine monochloride (ICl) are sensitive to the alkyl substituents, and the positions and number of vinyl fluorine atoms. These perturbations influence the symmetry of the halonium ion intermediates, which can be determined by the distribution of the Markovnikov to anti-Markovnikov products. A vinyl fluorine on the number-2 carbon favors an unsymmetrical intermediate with greater charge on the number-2 carbon unless the alkyl group is electron withdrawing. A vinyl fluorine on the terminal number-1 carbon favors positive charge development on that carbon unless a resonance stabilizing group is on the number-2 carbon. The symmetry of halonium ions with vinyl fluorines on both carbons-1 and -2 depends primarily on the characteristics of the alkyl substituent. Intermediates range from open-ions with the positive charge on carbon-2, to various bridged species, to open-ions on the terminal carbon.