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1.
Int J Mol Sci ; 25(5)2024 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-38474242

RESUMO

Ceramides regulate phagocytosis; however, their exact function remains poorly understood. Here, we sought (1) to develop genetically encoded fluorescent tools for imaging ceramides, and (2) to use them to examine ceramide dynamics during phagocytosis. Fourteen enhanced green fluorescent protein (EGFP) fusion constructs based on four known ceramide-binding domains were generated and screened. While most constructs localized to the nucleus or cytosol, three based on the CA3 ceramide-binding domain of kinase suppressor of ras 1 (KSR1) localized to the plasma membrane or autolysosomes. C-terminally tagged CA3 with a vector-based (C-KSR) or glycine-serine linker (C-KSR-GS) responded sensitively and similarly to ceramide depletion and accumulation using a panel of ceramide modifying drugs, whereas N-terminally tagged CA3 (N-KSR) responded differently to a subset of treatments. Lipidomic and liposome microarray analysis suggested that, instead, N-KSR may preferentially bind glucosyl-ceramide. Additionally, the three probes showed distinct dynamics during phagocytosis. Despite partial autolysosomal degradation, C-KSR and C-KSR-GS accumulated at the plasma membrane during phagocytosis, whereas N-KSR did not. Moreover, the weak recruitment of C-KSR-GS to the endoplasmic reticulum and phagosomes was enhanced through overexpression of the endoplasmic reticulum proteins stromal interaction molecule 1 (STIM1) and Sec22b, and was more salient in dendritic cells. The data suggest these novel probes can be used to analyze sphingolipid dynamics and function in living cells.


Assuntos
Ceramidas , Corantes Fluorescentes , Proteínas Quinases , Ceramidas/metabolismo , Transdução de Sinais/fisiologia , Fagocitose
2.
EMBO J ; 36(12): 1736-1754, 2017 06 14.
Artigo em Inglês | MEDLINE | ID: mdl-28495678

RESUMO

Sphingolipids are membrane lipids globally required for eukaryotic life. The sphingolipid content varies among endomembranes with pre- and post-Golgi compartments being poor and rich in sphingolipids, respectively. Due to this different sphingolipid content, pre- and post-Golgi membranes serve different cellular functions. The basis for maintaining distinct subcellular sphingolipid levels in the presence of membrane trafficking and metabolic fluxes is only partially understood. Here, we describe a homeostatic regulatory circuit that controls sphingolipid levels at the trans-Golgi network (TGN). Specifically, we show that sphingomyelin production at the TGN triggers a signalling pathway leading to PtdIns(4)P dephosphorylation. Since PtdIns(4)P is required for cholesterol and sphingolipid transport to the trans-Golgi network, PtdIns(4)P consumption interrupts this transport in response to excessive sphingomyelin production. Based on this evidence, we envisage a model where this homeostatic circuit maintains a constant lipid composition in the trans-Golgi network and post-Golgi compartments, thus counteracting fluctuations in the sphingolipid biosynthetic flow.


Assuntos
Fosfatidilinositóis/metabolismo , Esfingolipídeos/metabolismo , Rede trans-Golgi/metabolismo , Células HeLa , Homeostase , Humanos , Modelos Biológicos
3.
Metab Eng ; 37: 46-62, 2016 09.
Artigo em Inglês | MEDLINE | ID: mdl-27113440

RESUMO

We present a model-based method, designated Inverse Metabolic Control Analysis (IMCA), which can be used in conjunction with classical Metabolic Control Analysis for the analysis and design of cellular metabolism. We demonstrate the capabilities of the method by first developing a comprehensively curated kinetic model of sphingolipid biosynthesis in the yeast Saccharomyces cerevisiae. Next we apply IMCA using the model and integrating lipidomics data. The combinatorial complexity of the synthesis of sphingolipid molecules, along with the operational complexity of the participating enzymes of the pathway, presents an excellent case study for testing the capabilities of the IMCA. The exceptional agreement of the predictions of the method with genome-wide data highlights the importance and value of a comprehensive and consistent engineering approach for the development of such methods and models. Based on the analysis, we identified the class of enzymes regulating the distribution of sphingolipids among species and hydroxylation states, with the D-phospholipase SPO14 being one of the most prominent. The method and the applications presented here can be used for a broader, model-based inverse metabolic engineering approach.


Assuntos
Análise do Fluxo Metabólico/métodos , Metaboloma/fisiologia , Modelos Biológicos , Fosfolipase D/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/metabolismo , Esfingolipídeos/metabolismo , Simulação por Computador , Perfilação da Expressão Gênica/métodos , Cinética , Engenharia Metabólica/métodos , Redes e Vias Metabólicas/fisiologia , Fosfolipase D/genética , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/genética , Esfingolipídeos/genética
4.
PLoS Genet ; 7(10): e1002332, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22028670

RESUMO

Using small molecule probes to understand gene function is an attractive approach that allows functional characterization of genes that are dispensable in standard laboratory conditions and provides insight into the mode of action of these compounds. Using chemogenomic assays we previously identified yeast Crg1, an uncharacterized SAM-dependent methyltransferase, as a novel interactor of the protein phosphatase inhibitor cantharidin. In this study we used a combinatorial approach that exploits contemporary high-throughput techniques available in Saccharomyces cerevisiae combined with rigorous biological follow-up to characterize the interaction of Crg1 with cantharidin. Biochemical analysis of this enzyme followed by a systematic analysis of the interactome and lipidome of CRG1 mutants revealed that Crg1, a stress-responsive SAM-dependent methyltransferase, methylates cantharidin in vitro. Chemogenomic assays uncovered that lipid-related processes are essential for cantharidin resistance in cells sensitized by deletion of the CRG1 gene. Lipidome-wide analysis of mutants further showed that cantharidin induces alterations in glycerophospholipid and sphingolipid abundance in a Crg1-dependent manner. We propose that Crg1 is a small molecule methyltransferase important for maintaining lipid homeostasis in response to drug perturbation. This approach demonstrates the value of combining chemical genomics with other systems-based methods for characterizing proteins and elucidating previously unknown mechanisms of action of small molecule inhibitors.


Assuntos
Anticarcinógenos/metabolismo , Cantaridina/metabolismo , Metabolismo dos Lipídeos/genética , Metiltransferases/genética , Metiltransferases/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/enzimologia , Actinas/metabolismo , Animais , Anticarcinógenos/farmacologia , Cantaridina/análogos & derivados , Cantaridina/farmacologia , Parede Celular/genética , Parede Celular/metabolismo , Besouros/química , Citoesqueleto/metabolismo , Glicerofosfolipídeos/metabolismo , Homeostase/genética , Redes e Vias Metabólicas , Metilação , Mutagênese Sítio-Dirigida , Fosfoproteínas Fosfatases/antagonistas & inibidores , Fosfoproteínas Fosfatases/genética , Fosfoproteínas Fosfatases/metabolismo , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/genética , Esfingolipídeos/metabolismo , Estresse Fisiológico/genética , Biologia de Sistemas/métodos
5.
Artigo em Inglês | MEDLINE | ID: mdl-39094252

RESUMO

Sphingolipids are a major lipid species found in all eukaryotes. Among structurally complex and diversified lipids, sphingoid bases have been heavily linked to various metabolic diseases. However, most current LC-MS-based methods lack the sensitivity to detect low-abundant sphingoid bases. The 6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatization reagent, which efficiently forms covalent bonds with amino groups, has been widely used for amino acid detection. Nevertheless, the commonly used reverse-phase HPLC method for amino acid analysis is not suitable for amphipathic sphingolipids. To address this issue, we report a robust reverse-phase HPLC-MS/MS method capable of separating and detecting hydrophilic amino acids and sphingoid bases in a single run with high sensitivity. This method is also inclusive of other amino metabolites with an expandable target list. We tested this method under various conditions and samples, demonstrating its high reproducibility and sensitivity. Using this approach, we systematically analyzed human serum samples from healthy individuals, dyslipidemia, and type II diabetes mellitus (T2DM) patients, respectively. Two sphingolipids and five amino acids were identified with significant differences between the control and T2DM groups, highlighting the potential of this method in clinical studies.


Assuntos
Aminoácidos , Esfingolipídeos , Espectrometria de Massas em Tandem , Espectrometria de Massas em Tandem/métodos , Humanos , Esfingolipídeos/sangue , Esfingolipídeos/química , Reprodutibilidade dos Testes , Aminoácidos/sangue , Aminoácidos/química , Cromatografia Líquida de Alta Pressão/métodos , Carbamatos/sangue , Carbamatos/química , Aminoquinolinas/química , Aminoquinolinas/sangue , Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/metabolismo , Limite de Detecção , Modelos Lineares , Sensibilidade e Especificidade , Espectrometria de Massa com Cromatografia Líquida
6.
Development ; 137(21): 3675-85, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-20940226

RESUMO

The high sterol concentration in eukaryotic cell membranes is thought to influence membrane properties such as permeability, fluidity and microdomain formation. Drosophila cannot synthesize sterols, but do require them for development. Does this simply reflect a requirement for sterols in steroid hormone biosynthesis, or is bulk membrane sterol also essential in Drosophila? If the latter is true, how do they survive fluctuations in sterol availability and maintain membrane homeostasis? Here, we show that Drosophila require both bulk membrane sterol and steroid hormones in order to complete adult development. When sterol availability is restricted, Drosophila larvae modulate their growth to maintain membrane sterol levels within tight limits. When dietary sterol drops below a minimal threshold, larvae arrest growth and development in a reversible manner. Strikingly, membrane sterol levels in arrested larvae are dramatically reduced (dropping sixfold on average) in most tissues except the nervous system. Thus, sterols are dispensable for maintaining the basic membrane biophysical properties required for cell viability; these functions can be performed by non-sterol lipids when sterols are unavailable. However, bulk membrane sterol is likely to have essential functions in specific tissues during development. In tissues in which sterol levels drop, the overall level of sphingolipids increases and the proportion of different sphingolipid variants is altered. These changes allow survival, but not growth, when membrane sterol levels are low. This relationship between sterols and sphingolipids could be an ancient and conserved principle of membrane homeostasis.


Assuntos
Drosophila/crescimento & desenvolvimento , Drosophila/metabolismo , Esteróis/metabolismo , Animais , Animais Geneticamente Modificados , Membrana Celular/metabolismo , Sobrevivência Celular , Células Cultivadas , Drosophila/embriologia , Drosophila/fisiologia , Embrião não Mamífero , Hormônios/metabolismo , Larva/crescimento & desenvolvimento , Larva/metabolismo , Modelos Biológicos , Esfingolipídeos/metabolismo , Esteroides/metabolismo , Sobrevida/fisiologia
7.
Nat Cell Biol ; 25(8): 1157-1172, 2023 08.
Artigo em Inglês | MEDLINE | ID: mdl-37400497

RESUMO

Lipid mobilization through fatty acid ß-oxidation is a central process essential for energy production during nutrient shortage. In yeast, this catabolic process starts in the peroxisome from where ß-oxidation products enter mitochondria and fuel the tricarboxylic acid cycle. Little is known about the physical and metabolic cooperation between these organelles. Here we found that expression of fatty acid transporters and of the rate-limiting enzyme involved in ß-oxidation is decreased in cells expressing a hyperactive mutant of the small GTPase Arf1, leading to an accumulation of fatty acids in lipid droplets. Consequently, mitochondria became fragmented and ATP synthesis decreased. Genetic and pharmacological depletion of fatty acids phenocopied the arf1 mutant mitochondrial phenotype. Although ß-oxidation occurs in both mitochondria and peroxisomes in mammals, Arf1's role in fatty acid metabolism is conserved. Together, our results indicate that Arf1 integrates metabolism into energy production by regulating fatty acid storage and utilization, and presumably organelle contact sites.


Assuntos
Mitocôndrias , Peroxissomos , Animais , Mitocôndrias/metabolismo , Peroxissomos/metabolismo , Ácidos Graxos/metabolismo , Oxirredução , Metabolismo dos Lipídeos/genética , Homeostase , Mamíferos/metabolismo
8.
J Lipid Res ; 53(3): 412-420, 2012 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22210926

RESUMO

Sphingolipids are not only important components of membranes but also have functions in protein trafficking and intracellular signaling. The LCB1 gene encodes a subunit of the serine palmitoyltransferase, which is responsible for the first step of sphingolipid synthesis. Here, we show that activation of the unfolded protein response (UPR) can restore normal ceramide levels and viability in yeast cells with a conditional defect in LCB1. Dependence on UPR was demonstrated by showing the HAC1-dependence of the suppression. A similar induction of ceramides by UPR seems to take place in mammalian cells. In rat pancreatic INS-1E cells, UPR activation induces the transcription of the CerS6 gene, which encodes a ceramide synthase. This correlates with the specific accumulation of ceramide with a C16 fatty acyl chain upon UPR activation. Therefore, our study reveals a novel connection between UPR induction and ceramide synthesis that seems to be conserved between yeast and mammalian cells.


Assuntos
Ceramidas/metabolismo , Insulinoma/metabolismo , Saccharomyces cerevisiae/metabolismo , Resposta a Proteínas não Dobradas/fisiologia , Animais , Linhagem Celular Tumoral , Estresse do Retículo Endoplasmático/genética , Estresse do Retículo Endoplasmático/fisiologia , Oxirredutases/genética , Oxirredutases/metabolismo , Ratos , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/metabolismo , Espectrometria de Massas por Ionização por Electrospray , Esfingomielinas/metabolismo , Resposta a Proteínas não Dobradas/genética , Proteínas de Transporte Vesicular/genética , Proteínas de Transporte Vesicular/metabolismo
9.
J Biol Chem ; 286(13): 11401-14, 2011 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-21303904

RESUMO

Although the yeast Saccharomyces cerevisiae has only one sphingolipid class with a head group based on phosphoinositol, the yeast Pichia pastoris as well as many other fungi have a second class, glucosylceramide, which has a glucose head group. These two sphingolipid classes are in addition distinguished by a characteristic structure of their ceramide backbones. Here, we investigate the mechanisms controlling substrate entry into the glucosylceramide branch of the pathway. By a combination of enzymatic in vitro studies and lipid analysis of genetically engineered yeast strains, we show that the ceramide synthase Bar1p occupies a key branching point in sphingolipid biosynthesis in P. pastoris. By preferring dihydroxy sphingoid bases and C(16)/C(18) acyl-coenzyme A as substrates, Bar1p produces a structurally well defined group of ceramide species, which is the exclusive precursor for glucosylceramide biosynthesis. Correlating with the absence of glucosylceramide in this yeast, a gene encoding Bar1p is missing in S. cerevisiae. We could not successfully investigate the second ceramide synthase in P. pastoris that is orthologous to S. cerevisiae Lag1p/Lac1p. By analyzing the ceramide and glucosylceramide species in a collection of P. pastoris knock-out strains in which individual genes encoding enzymes involved in glucosylceramide biosynthesis were systematically deleted, we show that the ceramide species produced by Bar1p have to be modified by two additional enzymes, sphingolipid Δ4-desaturase and fatty acid α-hydroxylase, before the final addition of the glucose head group by the glucosylceramide synthase. Together, this set of four enzymes specifically defines the pathway leading to glucosylceramide biosynthesis.


Assuntos
Proteínas Fúngicas/metabolismo , Genes Fúngicos/fisiologia , Oxirredutases/metabolismo , Pichia/metabolismo , Esfingolipídeos/biossíntese , Proteínas Fúngicas/genética , Oxirredutases/genética , Pichia/genética , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Especificidade da Espécie , Esfingolipídeos/genética
10.
Metab Eng ; 13(5): 555-69, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21741494

RESUMO

Sterols are major lipids in eukaryotes and differ in their specific structure between species. Both cholesterol and ergosterol can form liquid ordered domains in artificial membranes. We reasoned that substituting the main sterol ergosterol by cholesterol in yeast should permit domain formation and discriminate between physical and sterol structure-dependent functions. Using a cholesterol-producing yeast strain, we show that solute transporters for tryptophan and arginine are functional, whereas the export of weak organic acids via Pdr12p, a multi-drug resistance family member, is not. The latter reveals a sterol function that is probably dependent upon a precise sterol structure. We present a series of novel yeast strains with different sterol compositions as valuable tools to characterize sterol function and use them to refine the sterol requirements for Pdr12p. These strains will also be improved hosts for heterologous expression of sterol-dependent proteins and safe sources to obtain pure cholesterol and other sterols.


Assuntos
Colesterol/biossíntese , Colesterol/genética , Ergosterol , Saccharomyces cerevisiae/crescimento & desenvolvimento , Saccharomyces cerevisiae/metabolismo , Transportadores de Cassetes de Ligação de ATP/genética , Transportadores de Cassetes de Ligação de ATP/metabolismo , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/metabolismo , Triptofano/genética , Triptofano/metabolismo
11.
PLoS One ; 16(8): e0256184, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34388214

RESUMO

In eukaryotic cells, a subset of cell surface proteins is attached by the glycolipid glycosylphosphatidylinositol (GPI) to the external leaflet of the plasma membrane where they play important roles as enzymes, receptors, or adhesion molecules. Here we present a protocol for purification and mass spectrometry analysis of the lipid moiety of individual GPI-anchored proteins (GPI-APs) in yeast. The method involves the expression of a specific GPI-AP tagged with GFP, solubilization, immunoprecipitation, separation by electrophoresis, blotting onto PVDF, release and extraction of the GPI-lipid moiety and analysis by mass spectrometry. By using this protocol, we could determine the precise GPI-lipid structure of the GPI-AP Gas1-GFP in a modified yeast strain. This protocol can be used to identify the lipid composition of the GPI anchor of distinct GPI-APs from yeast to mammals and can be adapted to determine other types of protein lipidation.


Assuntos
Ceramidas/isolamento & purificação , Proteínas Ligadas por GPI/química , Glicosilfosfatidilinositóis/química , Glicoproteínas de Membrana/química , Proteínas de Saccharomyces cerevisiae/química , Saccharomyces cerevisiae/química , Membrana Celular/química , Membrana Celular/metabolismo , Ceramidas/química , Proteínas Ligadas por GPI/metabolismo , Genes Reporter , Glicosilfosfatidilinositóis/metabolismo , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Espectrometria de Massas , Glicoproteínas de Membrana/metabolismo , Saccharomyces cerevisiae/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo
12.
Elife ; 92020 09 28.
Artigo em Inglês | MEDLINE | ID: mdl-32985977

RESUMO

Recognition of conserved microbial molecules activates immune responses in plants, a process termed pattern-triggered immunity (PTI). Similarly, insect eggs trigger defenses that impede egg development or attract predators, but information on the nature of egg-associated elicitors is scarce. We performed an unbiased bioactivity-guided fractionation of eggs of the butterfly Pieris brassicae. Nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry of active fractions led to the identification of phosphatidylcholines (PCs). PCs are released from insect eggs, and they induce salicylic acid and H2O2 accumulation, defense gene expression and cell death in Arabidopsis, all of which constitute a hallmark of PTI. Active PCs contain primarily C16 to C18-fatty acyl chains with various levels of desaturation, suggesting a relatively broad ligand specificity of cell-surface receptor(s). The finding of PCs as egg-associated molecular patterns (EAMPs) illustrates the acute ability of plants to detect conserved immunogenic patterns from their enemies, even from seemingly passive structures such as eggs.


Assuntos
Arabidopsis , Borboletas/metabolismo , Óvulo/química , Fosfatidilcolinas , Animais , Arabidopsis/efeitos dos fármacos , Arabidopsis/imunologia , Larva/química , Fosfatidilcolinas/imunologia , Fosfatidilcolinas/metabolismo , Fosfatidilcolinas/farmacologia
13.
Curr Biol ; 28(12): 1950-1958.e6, 2018 06 18.
Artigo em Inglês | MEDLINE | ID: mdl-29887313

RESUMO

Coat protein complex II (COPII) proteins form vesicles from the endoplasmic reticulum to export cargo molecules to the Golgi apparatus. Among the many proteins involved in this process, Sec12 is a key regulator, functioning as the guanosine diphosphate (GDP) exchange factor for Sar1p, the small guanosine triphosphatase (GTPase) that initiates COPII assembly. Here we show that overexpression of phospholipase B3 in the thermosensitive sec12-4 mutant partially restores growth and protein transport at non-permissive temperatures. Lipidomics analyses of these cells show a higher content of lysophosphatidylinositol (lysoPI), consistent with the lipid specificity of PLB3. Furthermore, we show that lysoPI is specifically enriched in COPII vesicles isolated from in vitro budding assays. As these results suggested that lysophospholipids could facilitate budding under conditions of defective COPII coat dynamics, we reconstituted COPII binding onto giant liposomes with purified proteins and showed that lysoPI decreases membrane rigidity and enhances COPII recruitment to liposomes. Our results support a mechanical facilitation of COPII budding by lysophospholipids.


Assuntos
Vesículas Revestidas pelo Complexo de Proteína do Envoltório/metabolismo , Lisofosfolipídeos/metabolismo , Saccharomyces cerevisiae/metabolismo , Lisofosfolipídeos/genética , Microssomos/metabolismo
14.
J Cell Biol ; 217(3): 959-974, 2018 03 05.
Artigo em Inglês | MEDLINE | ID: mdl-29279306

RESUMO

The endoplasmic reticulum (ER)-mitochondrial encounter structure (ERMES) physically links the membranes of the ER and mitochondria in yeast. Although the ER and mitochondria cooperate to synthesize glycerophospholipids, whether ERMES directly facilitates the lipid exchange between the two organelles remains controversial. Here, we compared the x-ray structures of an ERMES subunit Mdm12 from Kluyveromyces lactis with that of Mdm12 from Saccharomyces cerevisiae and found that both Mdm12 proteins possess a hydrophobic pocket for phospholipid binding. However in vitro lipid transfer assays showed that Mdm12 alone or an Mmm1 (another ERMES subunit) fusion protein exhibited only a weak lipid transfer activity between liposomes. In contrast, Mdm12 in a complex with Mmm1 mediated efficient lipid transfer between liposomes. Mutations in Mmm1 or Mdm12 impaired the lipid transfer activities of the Mdm12-Mmm1 complex and furthermore caused defective phosphatidylserine transport from the ER to mitochondrial membranes via ERMES in vitro. Therefore, the Mmm1-Mdm12 complex functions as a minimal unit that mediates lipid transfer between membranes.


Assuntos
Retículo Endoplasmático/metabolismo , Kluyveromyces/metabolismo , Proteínas de Membrana/metabolismo , Membranas Mitocondriais/metabolismo , Proteínas Mitocondriais/metabolismo , Complexos Multiproteicos/metabolismo , Fosfolipídeos/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/metabolismo , Transporte Biológico Ativo/fisiologia , Retículo Endoplasmático/genética , Kluyveromyces/genética , Proteínas de Membrana/genética , Proteínas Mitocondriais/genética , Complexos Multiproteicos/genética , Fosfolipídeos/genética , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/genética , Relação Estrutura-Atividade
15.
Cancer Cell ; 32(6): 807-823.e12, 2017 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-29232555

RESUMO

Dysregulated mammalian target of rapamycin (mTOR) promotes cancer, but underlying mechanisms are poorly understood. We describe an mTOR-driven mouse model that displays hepatosteatosis progressing to hepatocellular carcinoma (HCC). Longitudinal proteomic, lipidomics, and metabolomic analyses revealed that hepatic mTORC2 promotes de novo fatty acid and lipid synthesis, leading to steatosis and tumor development. In particular, mTORC2 stimulated sphingolipid (glucosylceramide) and glycerophospholipid (cardiolipin) synthesis. Inhibition of fatty acid or sphingolipid synthesis prevented tumor development, indicating a causal effect in tumorigenesis. Increased levels of cardiolipin were associated with tubular mitochondria and enhanced oxidative phosphorylation. Furthermore, increased lipogenesis correlated with elevated mTORC2 activity and HCC in human patients. Thus, mTORC2 promotes cancer via formation of lipids essential for growth and energy production.


Assuntos
Carcinogênese/metabolismo , Carcinoma Hepatocelular/metabolismo , Fígado Gorduroso/metabolismo , Lipogênese/fisiologia , Neoplasias Hepáticas/metabolismo , Alvo Mecanístico do Complexo 2 de Rapamicina/metabolismo , Animais , Carcinoma Hepatocelular/etiologia , Transformação Celular Neoplásica/metabolismo , Fígado Gorduroso/complicações , Humanos , Lipídeos/biossíntese , Neoplasias Hepáticas/etiologia , Camundongos , Camundongos Knockout
16.
Mol Biol Cell ; 25(20): 3234-46, 2014 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-25143408

RESUMO

The regulatory pathways required to maintain eukaryotic lipid homeostasis are largely unknown. We developed a systematic approach to uncover new players in the regulation of lipid homeostasis. Through an unbiased mass spectrometry-based lipidomic screening, we quantified hundreds of lipid species, including glycerophospholipids, sphingolipids, and sterols, from a collection of 129 mutants in protein kinase and phosphatase genes of Saccharomyces cerevisiae. Our approach successfully identified known kinases involved in lipid homeostasis and uncovered new ones. By clustering analysis, we found connections between nutrient-sensing pathways and regulation of glycerophospholipids. Deletion of members of glucose- and nitrogen-sensing pathways showed reciprocal changes in glycerophospholipid acyl chain lengths. We also found several new candidates for the regulation of sphingolipid homeostasis, including a connection between inositol pyrophosphate metabolism and complex sphingolipid homeostasis through transcriptional regulation of AUR1 and SUR1. This robust, systematic lipidomic approach constitutes a rich, new source of biological information and can be used to identify novel gene associations and function.


Assuntos
Glicerofosfolipídeos/metabolismo , Metabolismo dos Lipídeos , Monoéster Fosfórico Hidrolases/genética , Proteínas Quinases/genética , Proteínas de Saccharomyces cerevisiae/genética , Esfingolipídeos/metabolismo , Esteróis/metabolismo , Glicerofosfolipídeos/análise , Homeostase , Lipídeos/análise , Espectrometria de Massas , Mutação , Saccharomyces cerevisiae/genética , Esfingolipídeos/análise , Esteróis/análise
17.
PLoS One ; 8(12): e85519, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24392018

RESUMO

The endoplasmic reticulum (ER) is a large, multifunctional and essential organelle. Despite intense research, the function of more than a third of ER proteins remains unknown even in the well-studied model organism Saccharomyces cerevisiae. One such protein is Spf1, which is a highly conserved, ER localized, putative P-type ATPase. Deletion of SPF1 causes a wide variety of phenotypes including severe ER stress suggesting that this protein is essential for the normal function of the ER. The closest homologue of Spf1 is the vacuolar P-type ATPase Ypk9 that influences Mn(2+) homeostasis. However in vitro reconstitution assays with Spf1 have not yielded insight into its transport specificity. Here we took an in vivo approach to detect the direct and indirect effects of deleting SPF1. We found a specific reduction in the luminal concentration of Mn(2+) in ∆spf1 cells and an increase following it's overexpression. In agreement with the observed loss of luminal Mn(2+) we could observe concurrent reduction in many Mn(2+)-related process in the ER lumen. Conversely, cytosolic Mn(2+)-dependent processes were increased. Together, these data support a role for Spf1p in Mn(2+) transport in the cell. We also demonstrate that the human sequence homologue, ATP13A1, is a functionally conserved orthologue. Since ATP13A1 is highly expressed in developing neuronal tissues and in the brain, this should help in the study of Mn(2+)-dependent neurological disorders.


Assuntos
Transportadores de Cassetes de Ligação de ATP/metabolismo , Retículo Endoplasmático/metabolismo , Manganês/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/citologia , Saccharomyces cerevisiae/metabolismo , Transportadores de Cassetes de Ligação de ATP/genética , Adenosina Trifosfatases/metabolismo , Transporte Biológico , Proteínas de Ciclo Celular/metabolismo , Células HeLa , Homeostase , Humanos , Microssomos/metabolismo , Mutação , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/genética , Homologia de Sequência de Aminoácidos
18.
Nat Cell Biol ; 14(5): 542-7, 2012 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-22504275

RESUMO

The plasma membrane delimits the cell, and its integrity is essential for cell survival. Lipids and proteins form domains of distinct composition within the plasma membrane. How changes in plasma membrane composition are perceived, and how the abundance of lipids in the plasma membrane is regulated to balance changing needs remains largely unknown. Here, we show that the Slm1/2 paralogues and the target of rapamycin kinase complex 2 (TORC2) play a central role in this regulation. Membrane stress, induced by either inhibition of sphingolipid metabolism or by mechanically stretching the plasma membrane, redistributes Slm proteins between distinct plasma membrane domains. This increases Slm protein association with and activation of TORC2, which is restricted to the domain known as the membrane compartment containing TORC2 (MCT; ref. ). As TORC2 regulates sphingolipid metabolism, our discoveries reveal a homeostasis mechanism in which TORC2 responds to plasma membrane stress to mediate compensatory changes in cellular lipid synthesis and hence modulates the composition of the plasma membrane. The components of this pathway and their involvement in signalling after membrane stretch are evolutionarily conserved.


Assuntos
Estresse Oxidativo , Proteínas de Ligação a RNA/metabolismo , Esfingolipídeos/biossíntese , Serina-Treonina Quinases TOR/metabolismo , Membrana Celular/metabolismo , Humanos , Transporte Proteico
19.
Methods Enzymol ; 470: 369-91, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20946818

RESUMO

The systematic and quantitative analysis of the different lipid species within a cell or an organism has recently become possible and the general approach has been termed "lipidomics." Traditional methods of identification and quantification of lipid species were laborious processes and it was necessary to use a wide variety of techniques to analyse the different lipid species, especially concerning the assigning of particular acyl chain lengths, hydroxylations, and desaturations to the diverse lipid species. While it is still not possible to quantitatively analyze all lipid species in one fell swoop, great progress has been made with the intensive use of quantitative mass spectrometry approaches. It is now relatively simple to quantify most of the lipid species, including all of the major ones, in a yeast cell. Different degrees of sophistication of mass spectrometric analysis exist and the available techniques and instrumentation are evolving rapidly. Therefore, we have decided to present robust, simple methods to quantify the major yeast lipids by mass spectrometry that should be accessible to anyone who has access to a standard mass spectrometry equipment. The methods to identify and quantify yeast glycerophospholipids and sphingolipids involve electrospray ionization mass spectrometry using fragmentation to characterize the lipid species. A simplified gas chromatographic method is used to quantify the major sterols that occur in wild-type yeast cells and ergosterol biosynthesis mutants.


Assuntos
Lipídeos/análise , Espectrometria de Massas/métodos , Leveduras/química , Cromatografia Gasosa-Espectrometria de Massas , Glicerofosfolipídeos/análise , Glicerofosfolipídeos/química , Extração em Fase Sólida , Espectrometria de Massas por Ionização por Electrospray , Esfingolipídeos/análise , Esfingolipídeos/química , Espectrometria de Massas em Tandem
20.
Science ; 324(5925): 381-4, 2009 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-19372430

RESUMO

Oxygen deprivation is rapidly deleterious for most organisms. However, Caenorhabditis elegans has developed the ability to survive anoxia for at least 48 hours. Mutations in the DAF-2/DAF-16 insulin-like signaling pathway promote such survival. We describe a pathway involving the HYL-2 ceramide synthase that acts independently of DAF-2. Loss of the ceramide synthase gene hyl-2 results in increased sensitivity of C. elegans to anoxia. C. elegans has two ceramide synthases, hyl-1 and hyl-2, that participate in ceramide biogenesis and affect its ability to survive anoxic conditions. In contrast to hyl-2(lf) mutants, hyl-1(lf) mutants are more resistant to anoxia than normal animals. HYL-1 and HYL-2 have complementary specificities for fatty acyl chains. These data indicate that specific ceramides produced by HYL-2 confer resistance to anoxia.


Assuntos
Proteínas de Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/metabolismo , Caenorhabditis elegans/fisiologia , Hipóxia Celular , Ceramidas/fisiologia , Oxirredutases/genética , Oxirredutases/metabolismo , Oxigênio/fisiologia , Animais , Apoptose , Caenorhabditis elegans/citologia , Caenorhabditis elegans/genética , Ceramidas/biossíntese , Deleção de Genes , Genes de Helmintos , Mutação , Receptor de Insulina/genética , Receptor de Insulina/metabolismo , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/crescimento & desenvolvimento , Saccharomyces cerevisiae/fisiologia , Esfingomielinas/biossíntese , Esfingomielinas/fisiologia , Especificidade por Substrato , Transformação Genética , Transgenes
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