RESUMO
BACKGROUND: The purpose of this study was to compare The Binding Site serum free light chain assay on two analytical platforms; the Dade Behring BNII and the Olympus AU400. Reference intervals were subsequently established on each analyser. METHODS: In total, 112 serum samples routinely submitted to the laboratory for protein electrophoresis were used for the comparison study. Reference interval data was generated from 126 ostensibly healthy anonymous individuals. RESULTS: Serum free light chain results on the BNII and AU400 analysers are not directly comparable. The BNII produces results which are significantly higher than the AU400. CONCLUSIONS: Laboratories using The Binding Site serum free light chain assay are strongly advised to establish local reference intervals.