Revealing binding mechanism of ß-casein to chrysin, apigenin, and luteolin and locating its binding pockets by molecular docking and molecular dynamics.

Revealing the interaction mechanism of proteins with bioactive molecules and the location of their binding pockets is crucial for predicting the structure-function relationship of proteins in drug discovery and design. Despite some published papers on the interaction of ß-casein with small bioactive molecules, the ambiguity of the location and constituent amino acids of ß-casein binding pockets prompted us to identify them by in silico simulation of its interaction with three polyphenols, chrysin, apigenin, and luteolin. Molecular docking revealed that the primary ß-casein binding pocket for chrysin consists of five nonpolar amino acids (Leu73, Phe77, Pro80, Ile89, and Pro196), three polar neutral amino acids (Ser137, Gln138, and Gln197), and two polar charged amino acids (Glu136, and Arg198). For ß-casein/apigenin and ß-casein/luteolin complexes, Asn83 also contributes to forming the pocket. Molecular dynamics provided more details, such as the relative contribution of determinative amino acids and the role of various forces. For example, we found that Glu210, Glu132, and Glu35 are the most destructive residues in the binding of chrysin, apigenin, and luteolin to ß-casein, respectively. Also, we observed that hydrophobic forces mainly stabilize ß-casein/chrysin and ß-casein/apigenin, and polar solvation (including hydrogen bonds) stabilizes ß-casein/luteolin, all by spontaneous processes.

Excitation-independent deep-blue emitting carbon dots with 62% emission quantum efficiency and monoexponential decay profile for high-resolution fingerprint identification.

Reaching emissive nanomaterials at short wavelengths with a high quantum efficiency (QE) is an attractive task for researchers. This is more demanding in carbon dots (CDs) with diverse applications that usually emit photons at wavelengths around 450-620 nm. In this study, deep blue-emissive doped-CDs (d-CDs) with high photoluminescence (PL) QE up to 62% and excitation-independent properties were prepared via a short-time microwave irradiation method. The prepared CDs showed simultaneous amorphous and crystalline features, with average sizes of 4.75 nm and bright emission color located at 422 nm. It was found that the presence of sulfur-related dopant levels plays a key role in emission properties in such a way that the PL signal drops significantly in the absence of N-acetyl-l-cysteine (NAC) as a dopant source. On the other hand, the trisodium citrate dihydrate (TSC) was selected as a carbon source to form the main carbon skeleton without it no emission was recorded. Monoexponential-fitted recombination trend with an average lifetime of about 10 ns also confirmed excellent PL emission properties with uniform energy levels and minimized defect-contributing recombinations. The practical use of the as-prepared N, S-doped CDs was assessed in fingerprint detection indicating a bright and clear scheme for both core and termination regions of the fingerprint. Simplicity, cost-effectiveness, high-product yield, low toxicity, along with high/stable PL quantum efficiency in deep-blue wavelengths, and demonstrated ability for fingerprint purposes, support the prospective application of these dual doped-CDs for sensing and bioimaging applications.

Luminescent, low-toxic and stable gradient-alloyed Fe:ZnSe(S)@ZnSe(S) core:shell quantum dots as a sensitive fluorescent sensor for lead ions.

In this paper, an aqueous-based approach is introduced for facile, fast, and green synthesis of gradient-alloyed Fe-doped ZnSe(S)@ZnSe(S) core:shell quantum dots (QDs) with intense and stable emission. Co-utilization of co-nucleation and growth doping strategies, along with systematic optimization of emission intensity, provide a well-controllable/general method to achieve internally doped QDs (d-dots) with intense emission. Results indicate that the alloyed ZnSe(S)@ZnSe(S) core:shell QDs have a gradient structure that consists of a Se-rich core and a S-rich shell. This gradient structure cannot only passivate the core d-dots by means of the wider band gap S-rich shell, but also minimizes the lattice mismatch between alloyed core-shell structures. Using this novel strategy and utilizing the wider band gap S-rich shell can obviously increase the cyan emission intensity and also drastically improve the emission stability against chemical and optical corrosion. Furthermore, the cytotoxicity experiments indicate that the obtained d-dots are nontoxic nanomaterials, and thus they can be considered as a promising alternative to conventional Cd-based QDs for fluorescent probes in biological fields. Finally, it is demonstrated that the present low-toxicity and gradient-alloyed core:shell d-dots can be used as sensitive chemical detectors for Pb2+ ions with excellent selectivity, small detection limit, and rapid response time.

Facile, one-pot and scalable synthesis of highly emissive aqueous-based Ag,Ni:ZnCdS/ZnS core/shell quantum dots with high chemical and optical stability.

We report here on a one-pot, mild and low cost aqueous-based synthetic route for the preparation of colloidally stable and highly luminescent dual-doped Ag,Ni:ZnCdS/ZnS core/shell quantum dots (QDs). The pure dopant emission of the Ni-doped core/shell QDs was found to be highly affected by the presence of a second dopant ion (Ag+). Results showed that the PL emission intensity increases while its peak position experiences an obvious blue shift with an increase in the content of Ag+ ions. Regarding the optical observations, we provide a simple scheme for absorption-recombination processes of the carriers through impurity centers. To obtain optimum conditions with a better emission characteristic, we also study the effect of different reaction parameters, such as refluxing temperature, the pH of the core and shell solution, molar ratio of the dopant ions (Ni:(Zn+Cd) and Ag:(Zn+Cd)), and concentration of the core and shell precursors. Nonetheless, the most effective parameter is the presence of the ZnS shell in a suitable amount to eliminate surface trap states and enhance their emission intensity. It can also improve the bio-compatibility of the prepared QDs by restricting the Cd2+ toxic ions inside the core of the QDs. The present suggested route also revealed the remarkable optical and chemical stability of the colloidal QDs which establishes them as a decent kind of nano-scale structure for light emitting applications, especially in biological technologies. The suggested process also has the potential to be scaled-up while maintaining the emission characteristics and structural quality necessary for industrial applications in optoelectronic devices.

Preparation of Highly Biocompatible ZnSe Quantum Dots Using a New Source of Acetyl Cysteine as Capping Agent.

In this paper, we describe a facile method for preparation of ZnSe quantum dots (QDs) using an inexpensive and biocompatible source of acetyl cysteine in aqueous media. The structural properties of the ZnSe QDs have been characterized using XRD, FT-IR, and TEM techniques. The optical properties of the as-prepared QDs were found to be size-dependent, due to the strong confinement regime at relatively low refluxing time. Effect of solution pH and refluxing temperature on absorption and emission characteristics of the ZnSe QDs was studied. The empirical effective mass approximation also reveals that, both solution pH and refluxing temperature parameters would effect on ZnSe QDs growth, and increase their size. However, the influence of the solution pH was found to be more prominent. Water-solubility, high emission intensity and sub-10 nm nanocrystals size are the most essential features that suggest our synthesized aqueous-based ZnSe QDs (with a very cost-effective and biocompatible capping agent) can be utilized for biological intentions.

A new catalytic oxidation method for sensitive quantification of bromate in flours and bottled water using AgNPs.

In this paper, a simple and sensitive spectrophotometric method for the determination of nanomolar level of bromate, based on the catalytic effect of silver nanoparticles on the oxidation of acid red 14 by potassium bromate, is described. The reaction rate was monitored spectrophotometrically by measuring the decrease in absorbance of acid red 14 at 516 nm. The detection limit of the method was 8 ng/mL, and the linear range was between 15 and 130 ng/mL. The effects of acidity, concentration of reactants and reaction time, and external ions were also discussed. The optimum reaction conditions were fixed, and some kinetic parameters determined. The relative standard deviation for the determination of bromate at the concentration of 50 ng/mL was calculated to be 0.996 % (n = 10). The method has been successfully applied to the determination of bromate in flours and bottled waters.

Comparison of Thyroid Gland Sonography Index with Serum Antithyroid Peroxidase, Antithyroglobulin, and Thyroid Function Tests in Patients with Hashimoto Thyroiditis.

BACKGROUND: Ultrasound examination of the thyroid has emerged as a useful diagnostic and prognostic tool, along with measuring serum titers of anti-thyroid peroxidase (TPO), anti-thyroglobulin (Tg), and thyroid hormones, in patients with Hashimoto's thyroiditis. So, we aimed at considering correlations of ultrasonographic, antibodies, and thyroid hormone levels. MATERIALS AND METHODS: A total of 149 patients (118 females, 31 males; aged 18-60 years; mean age: 38.60 ± 8.03 years) who were diagnosed with Hashimoto's thyroiditis were enrolled in the study. The blood sample was taken to measure serum titers of free T3 (FT3) and T4 (FT4), TSH, anti-TPO, and anti-Tg antibody titers. The thyroid sonography of each patient was classified into one of the five grades by real-time ultrasound (US) based on echogenicity, thyroid size, and thyroid pattern. We evaluated whether there was a correlation between thyroid characteristics observed via ultrasound and serum levels of thyroid hormones, anti-TPO antibodies, and anti-Tg antibodies. RESULTS: Nodular structures were detected in 54 (36.2%) patients (38 micro-nodular and 16 macro-nodular). Echogenicity was recorded as isoechoic in 15(10.07%) and hypoechoic in 119 (79.87%) subjects. Euthyroid subjects had significantly thicker isthmus than overt and subclinical hypothyroid patients (P=0.018). Mean serum TSH, anti-Tg, and anti-TPO antibody titers showed a significant increase in patients with macro-nodules compared to those with micro-nodules and individuals without nodules (P0.05). The thickness of the isthmus had a significant negative correlation with FT4 (P=0.046; r=0.11) and FT3 (P=0.017; r=0.15), respectively. Thyroid autoantibodies had positive significant correlations with different parameters of thyroid volume (P0.05). CONCLUSIONS: Thyroid US findings, in addition to serum anti-Tg and anti-TPO antibody titers, might be correlated with the severity and extent of Hashimoto's thyroiditis, but further evaluations are needed.

Colloidal synthesis of tunably luminescent AgInS-based/ZnS core/shell quantum dots as biocompatible nano-probe for high-contrast fluorescence bioimaging.

Tremendous demands for simultaneous imaging of biological entities, along with the drawback of photobleaching in fluorescent dyes, have encouraged scientists to apply novel and non-toxic colloidal quantum dots (QDs) in biomedical researches. Herein, a novel aqueous-phase approach for the preparation of multicomponent In-based QDs is reported. Absorption and photoluminescence emission spectra of the as-prepared QDs were tuned by alteration of QDs' composition as Zn-Ag-In-S/ZnS, Ag-In-S/ZnS and Cu-Ag-In-S/ZnS core/shell QDs. In order to reach reproducibly intense and tunable light-emissive colloidal QDs with green, amber, and red color, various optimization steps were carefully performed. The structural characterizations such as EDX, ICP-AES, XRD, TEM and FT-IR measurements were also carried out to demonstrate the success of the present method to prepare extremely quantum-confined QDs capped with functional groups. Then, to ensure their promising biomedical applications, the generated intracellular reactive oxygen species (ROS) by QDs were quantitatively and qualitatively measured in dark conditions and under 405 nm laser irradiation. Our results verified an enhancement in the generation of reactive oxygen species (ROS) and cytotoxic effects in the presence of laser irradiation while their muted toxic effects in dark conditions confirmed biocompatible properties of un-excited In-based QDs. Moreover, bioimaging analysis revealed strong merits of the suggested synthetic route to achieve ideal fluorescent QDs as bright/multi-color optical nano-probes in imaging and transporting pumps in the cell membrane. This further emphasized the potential ability of the present AgInS-based/ZnS QDs in obtaining required results as theranostic agents for simultaneous treatment and imaging of cancer. The harmonized advantages in simplicity and effectiveness of synthesis procedure, excellent structural/optical properties enriched with confirmed biomedical merits in high contrast imaging and potential treatment highlight the present work.

AgNPs/QDs@GQDs nanocomposites developed as an ultrasensitive impedimetric aptasensor for ractopamine detection.

Developing easy-to-use and miniaturized sensors for in-field monitoring of targets which is related to human health is necessary. Ractopamine (RAC) is a feed additive with serious side effects that is forbidden in many countries. This study reports the fabrication of an impedimetric aptasensor for ultrasensitive and selective detection of the RAC in human biological fluids. Accordingly, an efficient nanocomposites was synthesized by a beneficial combination of graphene quantum dots (GQDs), quantum dots (QDs) and silver nanoparticles (AgNPs) for modifying a glassy carbon electrode (GCE). This nanocomposite is promising to present a synergistic effect in the increase of the active surface area of the modified electrode to more load the biocapture of the target. Next, the RAC-binding aptamer (Apt) was attached to the AgNPs/QDs@GQDs/GCE surface and a sensitive layer for the RAC detection was embedded. A RAC-Apt complex was formed upon adding the RAC and the changes of the electrochemical behavior were studied by some electrochemical techniques such as electrochemical impedance spectroscopy (EIS). Under optimal conditions, the charge transfer resistance (Rct) value was increased linearly with increasing of the RAC concentrations in the range of 1 fM to 901.4 nM. Limit of detection (LOD) was calculated to be 330 aM which is superior by other reported electrochemical methods in the RAC sensing. The applicability of the aptasensor was tested in human urine and blood serum as the real samples and satisfactory results of specificity were achieved. It seems that the proposed strategy not only provides a new ultrasensitive strategy for RAC detection but also expands the application of the sensing interface to develop other aptasensors by changing the Apt sequence.

An electrochemical tyrosinamide aptasensor using a glassy carbon electrode modified by N-acetyl-l-cysteine-capped Ag-In-S QDs.

This paper reports an aptamer-based green approach for the electrochemical evaluation of tyrosinamide (Tyr-NH2). In this regard, at the first step, an aqueous synthetic strategy for preparing N-acetyl-l-cysteine (NAC)-capped Ag-In-S (AIS) quantum dots (QDs) with bright yellow/orange emission was developed. The conjugation of AIS QDs to NAC-biomolecules provides opportunities for using them as luminescent contrast agents for living cell tracking and labeling or sensing studies. In the next step, the design stage of the aptasensor, the glassy carbon electrode (GCE) was modified with the AIS QDs and then the Tyr-NH2 special aptamer, which has an amine group at its end, interacts with silver and indium ions at the surface of the AIS QDs and through the formation of covalent bonding of AgN and InN, attaches to the GCE surface modified with the AIS QDs. In this approach, for the first time, NAC-capped AIS QDs have been used to modify the electrode surface in the aptamer-based electrochemical sensor. The response changes of the [Fe(CN)6]4-/3- as redox probe, during the modification of GCE surface, the fabrication and assessment of proposed aptasensing, using the cyclic voltammetry, differential pulse voltammetry and electrochemical impedance spectroscopy were recorded. The designed aptasensor for the Tyr-NH2 evaluation showed good linearity from 0.01 to 2.81 nM and 2.81-10.81 nM, and low detection limit of 3.34 pM. The obtained results of the stability, reproducibility and selectivity investigations implying that the reported aptasensor as the first aptamer-based electrochemical assay for Tyr-NH2, can be reliable for the determination of Tyr-NH2 in serum samples.

Synthesis and optimization of emission characteristics of water-dispersible ag-in-s quantum dots and their bactericidal activity.

Developing novel aqueous-soluble quantum dots (QDs) can create new opportunities for better biological utilization. In the present work, novel, high emissive and biocompatible N-acetyl-L-cysteine-capped Ag-In-S QDs (as an I-III-VI structure) were prepared in a facile and straightforward way. The dominance of the strong confinement regime was observed due to the very small size of nanoparticles, which was smaller than their excitonic Bohr radius. To prepare reproducible Ag-In-S QDs, their emission characteristics were improved by optimizing the experimental variables which resulted in the enhancement of their emission quantum yield to near 32% at 615 nm. The absorption and emission results support the contribution of band edge-independent radiative recombination pathways for charge carriers in the prepared Ag-In-S QDs. The possible mechanisms for such donor-acceptor recombination were also discussed. To explore the antibacterial ability of the Ag-In-S QDs, their bactericidal activity was evaluated against different types of Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Escherichia coli and Salmonella enterica) bacteria. Precise measurements confirmed a remarkable bactericidal activity of Ag-In-S QDs against the different pathogenic bacteria even at low concentration of QDs (15 µg/mL). It was found that the QDs are more effective on Gram-negative bacteria. While the preparation method was simple and cost-effective, the as-synthesized QDs were highly emissive and stable with significant antibacterial activity. This demonstrates the great potential of present Ag-In-S QDs for future hygienic and medical purposes.

Aqueous-based synthesis of Cd-free and highly emissive Fe-doped ZnSe(S)/ZnSe(S) core/shell quantum dots with antibacterial activity.

The effective insertion of intentional impurities in direct aqueous preparation of doped QDs still needs a chemical route with well-designed strategy. The present work reports a facile, one-pot, and aqueous-based method for green synthesis of Fe-doped ZnSe(S)/ZnSe(S) core/shell QDs with improved emission intensity. In the proposed strategy, by using a sulfur rich ZnSe(S) shell, we can provide a wider band gap shell with low structural defects in the interface between core and shell. Utilization of combined co-nucleation and growth doping strategies along with increasing the shell refluxing time all are the chemo-physical tactics which led to high intensity dopant-related emission. The antibacterial activity of the as-prepared doped core/shell QDs was investigated using agar disk diffusion method. The results show, these QDs have a significant antibacterial activity against different pathogenic bacteria comparing with the conventional antibiotics. The facility of suggested aqueous route for reaching a dopant emission, the bio-compatibility and considerable antibacterial characteristics of present QDs, nominate them as good candidates in further biological applications.

The Effect of Ondansetron and Dexamethasone on Nausea and Vomiting under Spinal Anesthesia.

BACKGROUND: During abdominal surgery under regional anesthesia, nausea may happen due to several contributing factors. This study compared the effects of ondansetron and dexamethasone on nausea and vomiting under spinal anesthesia. METHODS: One hundred and twenty patients of 15 to 35 years old with ASA class I and II were enrolled. Before administering either ondansetron or dexamethasone, blood pressure and heart rate of the patients were recorded. The patients received 70 mg of 5% lidocaine for spinal anesthesia. Patients who received 6 mg of ondansetron were considered as group A, while group B received 8 mg of dexamethasone. The level of nausea and vomiting, blood pressure, heart rate and respiratory rate of each patient was measured at 1, 5, 10, 15 and 30 minutes after spinal anesthesia and during recovery (every 5 minutes). RESULTS: There was a significant difference between nausea and vomiting between the two groups after spinal anesthesia within the first and fifth minutes. There was no significant difference between nausea and vomiting between the two groups within 10, 15 and 30 minutes and during recovery at 5, 10, 15 and 30 minutes. CONCLUSION: Dexamethasone and ondansetron were shown to equally reduce the incidence of nausea and vomiting under spinal anesthesia and can be recommended as a good choice for prevention of nausea and vomiting during surgeries.

A green ultrasonic-assisted liquid-liquid microextraction based on deep eutectic solvent for the HPLC-UV determination of ferulic, caffeic and cinnamic acid from olive, almond, sesame and cinnamon oil.

A simple, inexpensive and sensitive ultrasonic-assisted liquid-liquid microextraction method based on deep eutectic solvent (UALLME-DES) was used for the extraction of three phenolic acids (ferulic, caffeic and cinnamic) from vegetable oils. In a typical experiment, deep eutectic solvent as green extraction solvent was added to n-hexane (as a typical oil medium) containing target analytes. Subsequently, the extraction was accelerated by sonication. After the extraction, phase separation (DES rich phase/n-hexane phase) was performed by centrifugation. DES rich phase (lower phase) was withdrawn by a micro-syringe and submitted to isocratic reverse-phase HPLC with UV detection. Under optimum conditions obtained by response surface methodology (RSM) and desirability function (DF), the method has good linear calibration ranges (between 1.30 and 1000 µg L(-1)), coefficients of determination (r(2)>0.9949) and low limits of detection (between 0.39 and 0.63 µg L(-1)). This procedure was successfully applied to the determination of target analytes in olive, almond, sesame and cinnamon oil samples. The relative mean recoveries ranged from 94.7% to 104.6%.

Comparison of the Analgesic Effect of Paracetamol and Magnesium Sulfate during Surgeries.

BACKGROUND: New drugs are increasingly used to induce analgesia during surgeries. This study compared the analgesic effects of paracetamol and magnesium sulfate. METHODS: Sixty patients with American Society of Anesthesiologists (ASA) class I or II patients who were candidates for surgery of the lower limbs were randomly divided into three equal groups who were age and gender matched. Group 1 received paracetamol, and group 2, the magnesium sulfate during surgery and group 3 as the control. Pain intensities were measured and recorded using the Visual Analog Scale before surgery, in the recovery room, and 6, 12, and 18 hours after surgery. RESULTS: Pain intensities (7.10, 5.80, and 4.10) were higher in the control group; 6, 12, and 18 hours after surgery compared to the paracetamol (6.45, 4.15, 2.50) and the magnesium groups (7.25, 4.55, and 2.05), but the difference was not statistically significant. CONCLUSION: Paracetamol and magnesium sulfate were shown to have postoperative analgesic effects and reduce the quantity of narcotic use after surgery.

Digit-Length Ratios (2D:4D) as a Phenotypic Indicator of in Utero Androgen Exposure is Not Prognostic for Androgenic Alopecia: a Descriptive-Analytic Study of 1200 Iranian Men.

The etiology of androgenic alopecia (AGA) involves several factors, including genetics, androgens, age and nutrition. Digit-length ratio of the index and ring finger (2D:4D) is an indicator of prenatal exposure to sex hormones. There is a paucity of studies that systemically review the possible positive predictive value of 2D:4D in the development of AGA. We performed a single-site, descriptive-analytical study among a racially homogeneous population. Our results revealed that no significant association was determined between right 2D:4D and AGA severity within our entire population (P=0.384, r=0.025), however a positive correlation coefficient was identified in subjects above the age of 40. Based on the receiver operating characteristic curve analysis, 2D:4D does not predict the development of AGA. AGA is truly a multifactorial disease. Further, our findings suggest that increased in utero exposure to androgens as a fetus does not predispose men to develop AGA.

An investigation on optical characteristics of nanocrystalline ZnS:Ni thin films prepared by chemical deposition method.

Nanocrystalline nickel doped ZnS (ZnS:Ni) thin films were deposited by chemical bath deposition method. The effect of Ni doping on structural, photoluminescence, and optical properties of the ZnS:Ni films was studied. Structural analysis using X-ray diffraction revealed that the films are polycrystalline in nature with a cubic structure. The fluorescence (FL) spectra of the ZnS:Ni films showed two characteristic bands, one broad band centered at 430 and another narrow band at 523 nm. UV-vis transmission data showed that the films were transparent in the visible region. Also, using these data, the absorption coefficients, the optical band gap, the extinction coefficients, the refractive index, the real and imaginary parts of the dielectric constants, and the dissipation factor were calculated.

Emulsification liquid-liquid microextraction based on deep eutectic solvent: An extraction method for the determination of benzene, toluene, ethylbenzene and seven polycyclic aromatic hydrocarbons from water samples.

In this study, for the first time, a simple, inexpensive and sensitive method named emulsification liquid-liquid microextraction based on deep eutectic solvent (ELLME-DES) was used for the extraction of benzene, toluene, ethylbenzene (BTE) and seven polycyclic aromatic hydrocarbons (PAHs) from water samples. In a typical experiment, 100µL of DES (as water-miscible extraction solvent) was added to 1.5mL of sample solution containing target analytes. A homogeneous solution was formed immediately. Injection of 100µL of THF (as emulsifier agent) into homogeneous solution provided a turbid state. After extraction, phase separation (aqueous phase/DES rich phase) was performed by centrifugation. DES rich phase was withdrawn by a micro-syringe and submitted to isocratic reverse-phase HPLC with UV detection. Under optimum conditions obtained by response surface methodology (RSM) and desirability function (DF), the calibration graphs were linear in the concentration range from 10 to 200µg/L for benzene, 10-400µg/L for toluene, 1-400µg/L for ethylbenzene, biphenyl, chrysene and fluorene, and 0.1-400µg/L for anthracene, benzo[a]pyrene, phenanthrene and pyrene. The coefficients of determination (r(2)) and limits of detection were 0.9924-0.9997 and 0.02-6.8µg/L, respectively. This procedure was successfully applied to the determination of target analytes in spiked water samples. The relative mean recoveries ranged from 93.1 to 103.3%.

Ultrasound assisted microextraction-nano material solid phase dispersion for extraction and determination of thymol and carvacrol in pharmaceutical samples: experimental design methodology.

In the present study, for the first time, a new extraction method based on "ultrasound assisted microextraction-nanomaterial solid phase dispersion (UAME-NMSPD)" was developed to preconcentrate the low quantity of thymol and carvacrol in pharmaceutical samples prior to their HPLC-UV separation/determination. The analytes were accumulated on nickel sulfide nanomaterial loaded on activated carbon (NiS-NP-AC) that with more detail identified by XRD, FESEM and UV-vis technique. Central composite design (CCD) combined with desirability function (DF) was used to search for optimum operational conditions. Working under optimum conditions specified as: 10 min ultrasonic time, pH 3, 0.011 g of adsorbent and 600 µL extraction solvent) permit achievement of high and reasonable linear range over 0.005-2.0 µg mL(-1) (r(2)>0.9993) with LOD of thymol and carvacrol as 0.23 and 0.21 µg L(-1), respectively. The relative standard deviations (RSDs) were less than 4.93% (n=3).