Exploiting genotyping by sequencing to characterize the genomic structure of the American cranberry through high-density linkage mapping.

BACKGROUND: The application of genotyping by sequencing (GBS) approaches, combined with data imputation methodologies, is narrowing the genetic knowledge gap between major and understudied, minor crops. GBS is an excellent tool to characterize the genomic structure of recently domesticated (~200 years) and understudied species, such as cranberry (Vaccinium macrocarpon Ait.), by generating large numbers of markers for genomic studies such as genetic mapping. RESULTS: We identified 10842 potentially mappable single nucleotide polymorphisms (SNPs) in a cranberry pseudo-testcross population wherein 5477 SNPs and 211 short sequence repeats (SSRs) were used to construct a high density linkage map in cranberry of which a total of 4849 markers were mapped. Recombination frequency, linkage disequilibrium (LD), and segregation distortion at the genomic level in the parental and integrated linkage maps were characterized for first time in cranberry. SSR markers, used as the backbone in the map, revealed high collinearity with previously published linkage maps. The 4849 point map consisted of twelve linkage groups spanning 1112 cM, which anchored 2381 nuclear scaffolds accounting for ~13 Mb of the estimated 470 Mb cranberry genome. Bin mapping identified 592 and 672 unique bins in the parentals and a total of 1676 unique marker positions in the integrated map. Synteny analyses comparing the order of anchored cranberry scaffolds to their homologous positions in kiwifruit, grape, and coffee genomes provided initial evidence of homology between cranberry and closely related species. CONCLUSIONS: GBS data was used to rapidly saturate the cranberry genome with markers in a pseudo-testcross population. Collinearity between the present saturated genetic map and previous cranberry SSR maps suggests that the SNP locations represent accurate marker order and chromosome structure of the cranberry genome. SNPs greatly improved current marker genome coverage, which allowed for genome-wide structure investigations such as segregation distortion, recombination, linkage disequilibrium, and synteny analyses. In the future, GBS can be used to accelerate cranberry molecular breeding through QTL mapping and genome-wide association studies (GWAS).

Fragman: an R package for fragment analysis.

BACKGROUND: Determination of microsatellite lengths or other DNA fragment types is an important initial component of many genetic studies such as mutation detection, linkage and quantitative trait loci (QTL) mapping, genetic diversity, pedigree analysis, and detection of heterozygosity. A handful of commercial and freely available software programs exist for fragment analysis; however, most of them are platform dependent and lack high-throughput applicability. RESULTS: We present the R package Fragman to serve as a freely available and platform independent resource for automatic scoring of DNA fragment lengths diversity panels and biparental populations. The program analyzes DNA fragment lengths generated in Applied Biosystems® (ABI) either manually or automatically by providing panels or bins. The package contains additional tools for converting the allele calls to GenAlEx, JoinMap® and OneMap software formats mainly used for genetic diversity and generating linkage maps in plant and animal populations. Easy plotting functions and multiplexing friendly capabilities are some of the strengths of this R package. Fragment analysis using a unique set of cranberry (Vaccinium macrocarpon) genotypes based on microsatellite markers is used to highlight the capabilities of Fragman. CONCLUSION: Fragman is a valuable new tool for genetic analysis. The package produces equivalent results to other popular software for fragment analysis while possessing unique advantages and the possibility of automation for high-throughput experiments by exploiting the power of R.

Effects of Different Movement Programs on Motor Competence: A Systematic Review With Meta-Analysis.

BACKGROUND: To use the meta-analytic approach to assess the effectiveness of different types of movement programs on motor competence (MC) in participants of all ages. METHODS: Studies were retrieved by searching 13 databases and included when criteria were met. Studies were selected, and data were extracted by 2 authors. Random effects models using the standardized mean difference effect size (ES) were used to pool results. Risk of bias, heterogeneity, and inconsistency were examined. RESULTS: Thirty-six studies met the inclusion criteria. A total of 374 ESs were calculated and partitioned into 4 groups (motor intervention, free play, physical education classes, and control group). Statistically significant improvements in MC were observed for the motor intervention (ES = 1.50; 95% confidence interval [CI], 1.18 to 1.82; n = 36), as well as for free play (ES = 0.33; 95% CI, 0.09 to 0.57; n = 5), physical education classes (ES = 0.52; 95% CI, 0.08 to 0.97; n = 15), and smaller statistically significant differences in MC were observed for the control groups (ES = 0.16; 95% CI, 0.01 to 0.31; n = 6). CONCLUSIONS: All 4 groups analyzed improved MC in children, adolescents, and young adults. However, motor interventions were superior to all other groups for improving MC.

Expanding the theory of symptom modeling in patents with psychogenic nonepileptic seizures.

Symptom modeling has been used to explain the development of psychogenic nonepileptic seizures (PNES) in predisposed patients, but has been understood only in the context of individuals who have both PNES and epileptic seizures. Our aim in the study described here was to learn whether this concept applies to patients with PNES who do not have epileptic seizures, that is, to determine whether the prevalence of having personally witnessed a seizure prior to the occurrence of their event differs in patients with PNES and those with epileptic seizures. We interviewed 27 patients with PNES and 35 patients with epileptic seizures. A significantly larger number of patients with PNES (66%) admitted to having witnessed a seizure prior to their event compared with patients with epileptic seizures (11%) (P<0.001). This suggests that symptom modeling plays a key role in the development of PNES, even among individuals without a coexisting history of epilepsy.

Colecistectomía laparoscópica ambulatoria, una realidad actual / Ambulatory laparoscopic cholecystectomy, a current reality / Colecistectomia Laparoscópica Ambulatorial, uma realidade atual

La cirugía ambulatoria ha ido evolucionando y abarcando cada día procedimientos quirúrgicos más complejos, incluso la colecistectomía laparoscópica, actualmente considerada como la cirugía de elección para el tratamiento de la patología biliar, se puede realizar de manera ambulatoria gracias a los avances en técnicas quirúrgicas y anestésicas que actuales. Objetivo. Determinar la viabilidad y aceptación del procedimiento dentro de la practica ambulatoria mediante una revisión de la literatura actual sobre los protocolos de colecistectomía laparoscópica ambulatoria existentes. Metodología. Se realizó una revisión sistemática de la literatura, basado en la declaración PRISMA para ajustar la información obtenida en las diferentes bases científicas disponibles utilizando los descriptores relacionados con colecistectomía laparoscópica ambulatoria y cirugía del día enfocándose en aquellos registros sobre el tema, su conceptualización y puesta en marcha. De esta manera se identificaron 118 registros en las diferentes bases de datos consultadas. Tras la aplicación de criterios de inclusión se separaron 56 artículos de los cuales finalmente se utilizaron 11 registros para la revisión. Conclusión. La colecistectomía laparoscópica es segura y eficaz, siempre y cuando se realice la estatificación correcta del paciente a beneficiarse. No hay diferencia significativa entre los diferentes anestésicos utilizados durante el procedimiento, el anestésico subdérmico no mejora el tiempo de recuperación por dolor postoperatorio. La valoración protocolizada del postquirúrgico es vital para el alta hospitalaria.

Ambulatory surgery has been evolving and encompassing more complex surgical procedures every day. Even laparoscopic cholecystectomy, currently considered the surgery of choice for the treatment of biliary pathology, can be performed on an ambulatory basis thanks to advances in surgical and anesthetic techniques. Objective. To determine the feasibility and acceptance of the procedure in outpatient practice by reviewing the current literature on existing outpatient laparoscopic cholecystectomy protocols. Methodology. A systematic review of the literature was carried out, based on the PRISMA statement to adjust the information obtained in the different scientific bases available using the descriptors related to ambulatory laparoscopic cholecystectomy and day surgery focusing on those records on the subject, its conceptualization and implementation. In this way, 118 records were identified in the different databases consulted. After the application of inclusion criteria, 56 articles were separated from which 11 records were finally used for the review. Conclusion. Laparoscopic cholecystectomy is safe and effective, as long as the correct staging of the patient to be benefited is performed. There is no significant difference between the different anesthetics used during the procedure, the subdermal anesthetic does not improve postoperative pain recovery time. Protocolized postoperative assessment is vital for hospital discharge.

A cirurgia ambulatorial tem evoluído e engloba procedimentos cirúrgicos mais complexos a cada dia. Mesmo a colecistectomia laparoscópica, atualmente considerada a cirurgia de escolha para o tratamento da patologia biliar, pode ser realizada em regime ambulatorial graças aos avanços nas técnicas cirúrgicas e anestésicas. Objetivo. Determinar a viabilidade e aceitabilidade do procedimento na prática ambulatorial através de uma revisão da literatura atual sobre os protocolos existentes de coleistectomia laparoscópica ambulatorial. Metodologia. Foi realizada uma revisão sistemática da literatura, baseada na declaração PRISMA para ajustar as informações obtidas nas diferentes bases científicas disponíveis usando os descritores relacionados à coleistectomia laparoscópica ambulatorial e à cirurgia diurna, focalizando aqueles registros sobre o assunto, sua conceitualização e implementação. Desta forma, 118 registros foram identificados nos diferentes bancos de dados consultados. Após a aplicação dos critérios de inclusão, 56 artigos foram separados dos quais 11 registros foram finalmente utilizados para a revisão. Conclusão. A coleistectomia laparoscópica é segura e eficaz, desde que o estadiamento correto do paciente a ser beneficiado seja realizado. Não há diferença significativa entre os diferentes anestésicos utilizados durante o procedimento, a anestesia subdérmica não melhora o tempo de recuperação da dor pós-operatória. A avaliação pós-operatória protocolizada é vital para a alta hospitalar.

Práctica mental, física o combinada en el desempeño y aprendizaje motor: una respuesta meta-analítica / Mental, physical or combined practice in motor performance and learning: a meta-analytical response

Jiménez Díaz, J., Morera-Castro, M., Chaves-Castro, K., Portuguez-Molina, P., Araya-Vargas, G. y Salazar, W. (2022). Práctica mental, física o combinada en el desempeño y aprendizaje motor: una respuesta meta-analítica. PENSAR EN MOVIMIENTO: Revista de Ciencias del Ejercicio y la Salud, 20(2), 1-37. En el proceso de adquisición de destrezas motrices la práctica es un elemento fundamental por que facilita el aprendizaje; esta práctica puede ser de dos tipos: física o mental. El objetivo del presente estudio fue determinar el tamaño de efecto de la práctica física (PF), práctica mental (PM) y la práctica combinada (PC) a lo largo del proceso de adquisición y retención en el desempeño de las destrezas motrices, utilizando el enfoque meta-analítico y el análisis de posibles variables moderadoras. Se utilizó el modelo de efectos aleatorios para calcular el tamaño de efecto (TE). Se incluyeron 24 estudios y se obtuvo un total de 150 TE, separados en cuatro grupos. En la fase de adquisición se encontró que la PF (TE = 1.75; n = 36; IC = 0.96,3.55; Q = 113; I2 = 92%), la PM (TE = 0.80; n = 36; IC = 0.50,1.09; Q = 108; I 2= 74%) y la PC (TE = 1.78; n = 22; IC = 1.39,2.18; Q = 167; I2 = 79%) mejoraron su desempeño; además, el grupo control (GC) mejoró el desempeño en la adquisición (TE = 0.18; n = 22; IC = 0.02,0.35; Q = 19; I 2= 0%). Una metarregresión indicó que solo la práctica física, mental y combinada predicen significativamente el TE. En la fase de retención, ni la PF (TE = -0.97; n = 9; IC = -0.41,0.02; Q = 12; I2 = 29%), la PM (TE = 0.62; n = 6; IC = -0.19,1.45; Q = 22; I2 = 83%), la PC (TE = -0.01; n = 14; IC = -0.26,0.25; Q = 23; I2 = 46%), ni el GC (TE = -0.15; n = 5; IC = -0.75,0.44; Q = 11; I2 = 66%) presentaron un cambio significativo. Se analizaron las posibles variables moderadoras. Los resultados sugieren que los tres tipos de práctica mejoran en el desempeño en la fase de adquisición; mientras que, en la etapa de retención, favorecen a mantener lo aprendido. La PM, no reemplaza la PF; no obstante, bajo algunas condiciones, se puede complementar la PF con PM.

Jiménez Díaz, J., Morera-Castro, M., Chaves-Castro, K., Portuguez-Molina, P., Araya-Vargas, G. y Salazar, W. (2022). Mental, physical or combined practice in motor performance and learning: a meta-analytical response. PENSAR EN MOVIMIENTO: Revista de Ciencias del Ejercicio y la Salud, 20(2), 1-37. Practice is a key element in the process of acquiring motor skills, because it facilitates learning. This practice can be of either of two types—physical or mental. The objective of the present study is to determine the extent of effect of physical practice (PF), mental practice (PM) and combined practice (PC) throughout the process of acquisition and retention in the performance of motor skills, using a meta-analytical approach and the analysis of possible moderating variables. The random effect model was used to calculate the extent of the effect (TE). A total 24 studies were included, and a total of 150 TE was obtained, separated in four groups. In the phase of acquisition, it was found that the PF (TE = 1.75; n = 36; IC = 0.96,3.55; Q = 113; I2 = 92%), the PM (TE = 0.80; n = 36; IC = 0.50,1.09; Q = 108; I2 = 74%) and the PC (TE = 1.78; n = 22; IC = 1.39,2.18; Q = 167; I2 = 79%) improved their performance; in addition, the control group (GC) improved the performance in acquisition (TE = 0.18; n = 22; IC = 0.02,0.35; Q = 19; I2 = 0%). A meta-regression indicated that only physical, mental and combined practice significantly predict the TE. In the retention phase, neither the PF (TE = -0.97; n = 9; IC = -0.41,0.02; Q = 12; I2 = 29%), the PM (TE = 0.62; n = 6; IC = -0.19,1.45; Q = 22; I2 = 83%), the PC (TE = -0.01; n = 14; IC = -0.26,0.25; Q = 23; I2 = 46%) or the GC (TE = -0.15; n = 5; IC = -0.75,0.44; Q = 11; I2 = 66%) showed a significant change. Possible moderating variables were analyzed. The results suggest that the three types of practice improve performance in the acquisition phase, while in the retention phase they help to keep what has been learned. The PM does not replace PF; however, PF can be complemented with PM under some conditions.

Jiménez Díaz, J., Morera-Castro, M., Chaves-Castro, K., Portuguez-Molina, P., Araya-Vargas, G. y Salazar, W. (2022). Prática mental, física ou combinada no desempenho e na aprendizagem motora: uma resposta metanalítica. PENSAR EN MOVIMIENTO: Revista de Ciencias del Ejercicio y la Salud, 20(2), 1-37. No processo de aquisição de destrezas motoras, a prática é um elemento fundamental porque facilita o aprendizado; esta prática pode ser de dois tipos: física ou mental. O objetivo do presente estudo foi determinar o tamanho do efeito da prática física (PF), da prática mental (PM) e da prática combinada (PC) durante todo o processo de aquisição e retenção do desempenho das destrezas motoras, usando a abordagem metanalítica e a análise de possíveis variáveis moderadoras. Foi utilizado o modelo de efeitos aleatórios para calcular o tamanho do efeito (TDE). Vinte e quatro estudos foram incluídos e obtidos um total de 150 TDEs, separados em quatro grupos. Na fase de aquisição, PF (TDE = 1,75; n = 36; IC = 0,96,3,55; Q = 113; I2= 92%), PM (TDE = 0,80; n = 36; IC = 0,50,1,09; Q = 108; I 2= 74%) e CP(TDE = 1,78; n = 22; IC = 1,39,2,18; Q = 167; I 2 = 79%) melhoraram seu desempenho; além disso, o grupo de controle (GC) melhorou seu desempenho na aquisição(TDE = 0,18; n = 22; IC = 0,02,0,35; Q = 19; I 2= 0%). Uma meta-regressão indicou que apenas a prática física, mental e combinada prediz significativamente o TDE. Na fase de retenção, nem PF (TDE = -0,97; n = 9; IC = -0,41,0,02; Q = 12; I2= 29%), PM (TDE = 0,62; n = 6; IC = -0,19,1,45; Q = 22; I2 = 83%), PC (TDE = -0,01; n = 14; IC = -0,26,0,25; Q = 23; I2 = 46%), nem o GC (TDE = -0,15; n = 5; IC = -0,75,0,44; Q = 11; I2= 66%) apresentou uma mudança significativa. Foram analisadas as possíveis variáveis moderadoras. Os resultados indicam que os três tipos de prática melhoram o desempenho na fase de aquisição, enquanto na fase de retenção, eles melhoram a retenção do que foi aprendido. A PM não substitui a PF; no entanto, sob algumas condições, a PF pode ser complementada com a PM.

Trauma esplénico: diagnostico, clasificación y tratamiento. Una revisión de la literatura actual

Resumen El bazo es el órgano que se lesiona con más frecuencia en el trauma abdominal cerrado, presentándose en 30 a 50% de los casos, principalmente por su fragilidad y localización, su tratamiento ha estado en constante cambio a lo largo del tiempo, siendo hoy en día más utilizado el manejo conservador por sobre el intervencionista, considerando sobre todo el mayor riesgo de mortalidad y las condiciones fisiológicas posteriores a la esplenectomía principalmente en niños y adolescentes. Objetivo. Realizar una revisión actual del diagnóstico, clasificación y tratamiento del trauma esplénico. Metodología . Se realizó una revisión bibliográfica incluyendo los descriptores relacionados con trauma esplénico y su tratamiento. Resultados. La identificación del trauma esplénico es de vital importancia para la supervivencia del paciente, el diagnostico se puede realizar mediante estudios de imagen eco-FAST o tomografía en dependencia del estado hemodinámico del paciente, la clasificación depende de las características anatómicas de las lesiones y orienta el tratamiento adecuado. Conclusiones. Actualmente el tratamiento conservador está recomendado para lesiones I-III; los estadios mayores (IV y V) o cualquier grado siempre y cuando exista compromiso hemodinámico implican tratamiento intervencionista o quirúrgico, no existe una diferencia significativa entre la utilización de técnica abierta vs laparoscópica.

Abstract The spleen is the organ that is most frequently injured in blunt abdominal trauma, occurring in 30 to 50% of cases, mainly due to its fragility and location, its treatment has been in constant change over time, today the conservative management is more widely used than interventionist, considering above all the greater risk of mortality and the physiological conditions after splenectomy, mainly in children and adolescents. Objective . To carry out a current review of the diagnosis, classification and treatment of splenic trauma. Methodology . A bibliographic review was carried out including the descriptors related to splenic trauma and its treatment. Results .The identification of splenic trauma is of vital importance for the survival of the patient, the diagnosis can be made by imaging studies, FAST ultrasound or tomography depending on the hemodynamic status of the patient, the classification depends on the anatomical characteristics of the lesions and guides the appropriate treatment. Conclusions . Conservative treatment is currently recommended for lesions I-III; the major stages (IV and V) or any grade whit hemodynamic compromise imply interventional or surgical treatment, there is no significant difference between the use of open versus laparoscopic technique.

Resumo O baço é o órgão mais frequentemente lesado no trauma abdominal fechado, ocorrendo em 30 a 50% dos casos, principalmente devido à sua fragilidade e localização, seu tratamento tem mudado constantemente ao longo do tempo, sendo hoje o manejo conservador mais utilizado que o intervencionista, considerando sobretudo o maior risco de mortalidade e as condições fisiológicas após a esplenectomia, principalmente em crianças e adolescentes. Objetivo . Fazer uma revisão atual do diagnóstico, classificação e tratamento do trauma esplênico. Metodologia . Foi realizada uma revisão bibliográfica incluindo os descritores relacionados ao trauma esplênico e seu tratamento. Resultados . A identificação do trauma esplênico é de vital importância para a sobrevida do paciente, o diagnóstico pode ser feito por exames de imagem echo-FAST ou tomografia dependendo do estado hemodinâmico do paciente, a classificação depende das características anatômicas das lesões e orienta o tratamento adequado. Conclusões . O tratamento conservador é atualmente recomendado para lesões I-III; os estágios principais (IV e V) ou em qualquer grau, desde que haja comprometimento hemodinâmico, implicam em tratamento intervencionista ou cirúrgico, não havendo diferença significativa entre o uso da técnica aberta e laparoscópica.