RESUMO
Founder females of the leaf-cutting ant species Atta sexdens experience high mortality during the founding and establishment of their colonies. The foundation site is crucial for the success of a new colony. In this study, we isolated and identified actinobacteria from fungus garden chambers of A. sexdens colony growth in soils from (1) forested areas without leafcutter ant nests and (2) open ground areas close to leafcutter ant nests. The inhibitory effect of these isolates on pathogenic fungi and the mutualistic fungus cultivated by leafcutter ants was evaluated. The 16S rRNA gene sequences were employed to identify nine selected actinobacteria species found in the soil: Streptomyces (6), Nocardia (2), and Kitasatospora (1). One Streptomyces and one Kitasatospora isolate inhibited all the tested fungi. Since there is no evidence of actinobacteria cultivation in the workers' cuticle of the Atta genus, our results corroborate the hypothesis that these workers may establish temporary adaptive symbiosis with soil microorganisms that produce antibiotic substances, living in some parts of their nest, or even inside their bodies. Pathogenic fungi are a risk factor that can be controlled by actinobacteria metabolites from soils, with minimal energy cost to the colony.
Assuntos
Actinobacteria , Formigas , Actinobacteria/genética , Animais , Fungos/genética , Humanos , RNA Ribossômico 16S/genética , Solo , SimbioseRESUMO
The insecticide thiamethoxam (TMX) is a systemic neonicotinoid widely used for pest control in several agricultural crops. TMX mimics the action of acetylcholine causing uncontrolled muscular contraction eventually leading to insect death. TMX is being found in freshwater ecosystems at concentrations of up to 225µg/L. Still, chronic toxicity data for freshwater invertebrates is limited. Therefore, the aim of this study was to evaluate the acute and chronic effects (at organismal and biochemical levels) of TMX on the freshwater insect Chironomus riparius. C. riparius life history responses were significantly affected by TMX exposure, namely with a decrease in growth and delay in emergence. Concerning the biochemical responses, after a short exposure (48h) to TMX, our results showed that low concentrations of TMX significantly reduced CAT activity and LPO levels of C. riparius. No effects were observed in AChE, GST and ETS activities. Effects in terms of survival, development rates and biochemical responses of C. riparius exposed to low concentrations of TMX observed in this study suggest potential deleterious effects of this neonicotinoid on aquatic insects inhabiting freshwaters environments near agricultural areas.
Assuntos
Chironomidae/efeitos dos fármacos , Inseticidas/toxicidade , Nitrocompostos/toxicidade , Oxazinas/toxicidade , Tiazóis/toxicidade , Poluentes Químicos da Água/toxicidade , Análise de Variância , Animais , Chironomidae/crescimento & desenvolvimento , Água Doce/análise , Insetos/efeitos dos fármacos , Invertebrados/efeitos dos fármacos , Larva/efeitos dos fármacos , Larva/crescimento & desenvolvimento , Estágios do Ciclo de Vida/efeitos dos fármacos , Neonicotinoides , TiametoxamRESUMO
Crop management practices determine weed community, which in turn may influence patterns of diversity and abundance of associated arthropods. This study aimed to evaluate whether local weed management practices influence the diversity and relative abundance of phytophagous and predatory mites, as well as mites with undefined feeding habits--of the families Oribatidae and Acaridae--in a physic nut (Jatropha curcas L.) plantation subjected to (1) within-row herbicide spraying and between-row mowing; (2) within-row herbicide spraying and no between-row mowing; (3) within-row weeding and between-row mowing; (4) within-row weeding and no between-row mowing; and (5) unmanaged (control). The herbicide used was glyphosate. Herbicide treatments resulted in higher diversity and relative abundance of predatory mites and mites with undefined feeding habit on physic nut shrubs. This was probably due to the toxic effects of the herbicide on mites or to removal of weeds. Within-row herbicide spraying combined with between-row mowing was the treatment that most contributed to this effect. Our results show that within-row weeds harbor important species of predatory mites and mites with undefined feeding habit. However, the dynamics of such mites in the system can be changed according to the weed management practice applied. Among the predatory mites of the family Phytoseiidae Amblydromalus sp. was the most abundant, whereas Brevipalpus phoenicis was the most frequent phytophagous mite and an unidentified oribatid species was the most frequent mite with undefined feeding habit.
Assuntos
Biodiversidade , Glicina/análogos & derivados , Herbicidas , Ácaros , Controle de Plantas Daninhas/métodos , Animais , Comportamento Alimentar , Jatropha , Plantas Daninhas , GlifosatoRESUMO
Studies on the seasonal variation of agricultural pest species are important for the establishment of integrated pest control programs. The seasonality of pest attacks on crops is affected by biotic and abiotic factors, for example, climate and natural enemies. Besides that, characteristics of the host plant, crop management, location and the pests' bioecology also affect this seasonality. The mites Polyphagotarsonemus latus (Prostigmata: Tarsonemidae) and Tetranychus bastosi (Prostigmata: Tetranychidae) are the most important pests in the cultivation of physic nut, Jatropha curcas (Euphorbiaceae). All parts of J. curcas can be used for a wide range of purposes. In addition many researchers have studied its potential for use as neat oil, as transesterified oil (biodiesel), or as a blend with diesel. However studies about physic nut pests have been little known. The objective of this study was to assess the seasonal variation of P. latus and T. bastosi in physic nut. This study was conducted at three sites in the state of Tocantins, Brazil. We monitored climatic elements and the densities of the two mite species and of their natural enemies for a period of 2 years. Attack by P. latus occurred during rainy seasons, when the photoperiod was short and the physic nut had new leaves. In contrast, attack by T. bastosi occurred during warmer seasons with longer photoperiods and stronger winds. Populations of both mites and their natural enemies were greater in sites with greater plant diversity adjacent to the plantations. The predators found in association with P. latus and T. bastosi were Euseius concordis (Acari: Phytoseiidae), spiders, Stethorus sp. (Coleoptera: Coccinellidae) and Chrysoperla sp. (Neuroptera: Chrysopidae).
Assuntos
Agricultura , Jatropha/crescimento & desenvolvimento , Ácaros/fisiologia , Estações do Ano , Animais , Brasil , Cadeia Alimentar , Insetos/fisiologia , Dinâmica Populacional , Especificidade da Espécie , Aranhas/fisiologia , Tetranychidae/fisiologiaRESUMO
Both prey density and developmental stage of pests and natural enemies are known to influence the effectiveness of biological control. However, little is known about the interaction between prey density and population structure on predation and fecundity of generalist predatory mites. Here, we evaluated the functional response (number of prey eaten by predator in relation to prey density) of adult females and nymphs of the generalist predatory mite Euseius concordis to densities of different developmental stages of the cassava green mite Mononychellus tanajoa, as well as the fecundity of adult females of the predator. We further assessed the instantaneous rate of increase, based on fecundity and mortality, of E. concordis fed on eggs, immatures and adults of M. tanajoa. Overall, nymphs and adults of E. concordis feeding on eggs, immatures and females of M. tanajoa had a type III functional response curve suggesting that the predator increased prey consumption rate as prey density increased. Both nymphs and adult females of the predator consumed more eggs than immatures of M. tanajoa from the density of 20 items per leaf disc onwards, revealing an interaction between prey density and developmental stage in the predatory activity of E. concordis. In addition, population growth rate was higher when the predator fed on eggs and immatures in comparison with females. Altogether our results suggest that E. concordis may be a good candidate for the biological control of M. tanajoa populations. However, the efficiency of E. concordis as a biological control agent of M. tanajoa is contingent on prey density and population structure.
Assuntos
Ácaros/fisiologia , Comportamento Predatório , Tetranychidae/fisiologia , Animais , Feminino , Fertilidade , Ninfa/fisiologia , Controle Biológico de Vetores , Densidade Demográfica , Tetranychidae/crescimento & desenvolvimentoRESUMO
The starting point for generating a pest control decision-making system is a conventional sampling plan. Because the mites Polyphagotarsonemus latus and Tetranychus bastosi are among the most important pests of the physic nut (Jatropha curcas), in the present study, we aimed to establish sampling plans for these mite species on physic nut. Mite densities were monitored in 12 physic nut crops. Based on the obtained results, sampling of P. latus and T. bastosi should be performed by assessing the number of mites per cm(2) in 160 samples using a handheld 20× magnifying glass. The optimal sampling region for T. bastosi is the abaxial surface of the 4th most apical leaf on the branch of the middle third of the canopy. On the abaxial surface, T. bastosi should then be observed on the side parts of the middle portion of the leaf, near its edge. As for P. latus, the optimal sampling region is the abaxial surface of the 4th most apical leaf on the branch of the apical third of the canopy on the abaxial surface. Polyphagotarsonemus latus should then be assessed on the side parts of the leaf's petiole insertion. Each sampling procedure requires 4 h and costs US$ 7.31.
Assuntos
Jatropha/parasitologia , Controle de Pragas/métodos , Tetranychidae/crescimento & desenvolvimento , Animais , Brasil , Tomada de Decisões , Modelos Lineares , Folhas de Planta/parasitologiaRESUMO
Spontaneously growing plants are commonly considered competitors of cultivated plants. Owing to the lack of specificity of many arthropods, spontaneous plants may be attacked by the same arthropods that attack cultivated plants and they may also harbor natural enemies of organisms harmful to cultivated plants. Aceodromus convolvuli Muma (Blattisociidae) has been reported recently in relatively large numbers in Tocantins state, central Brazil, mostly on Helicteres guazumifolia Kunth (Malvaceae). Very little has been reported about the population dynamics of blattisociid mites under field conditions. The objective of this work was to study the population dynamics of A. convolvuli in Gurupi, Tocantins state, to evaluate its possible interaction with associated mites. Monthly samples were taken from leaves of the 11 most abundant and frequent spontaneous plants in a Jatropha curcas L. (Euphorbiaceae) plantation. About 96.5 % of the specimens of A. convolvuli were collected in the rainy season. The patterns of variation of the population of A. convolvuli and of predators belonging to the family Phytoseiidae were similar, but A. convolvuli was much more numerous than all phytoseiid specimens combined. Highly significant correlations were observed between A. convolvuli densities and relative humidity or diversity of spontaneous plants. When only mites on H. guazumifolia were considered, highly significant correlation was also observed between densities of A. convolvuli and of mites of the family Tetranychidae. The results suggested that A. convolvuli could be a predator of tenuipalpid and/or tetranychid mites. Studies about its biology are needed to determine its preferred food sources and potential as biological control agent.
Assuntos
Ácaros/fisiologia , Comportamento Predatório , Animais , Brasil , Ácaros/crescimento & desenvolvimento , Controle Biológico de Vetores , Plantas , Dinâmica Populacional , Estações do AnoRESUMO
Chlorpyrifos, an organophosphate insecticide widely used to control agricultural pests, poses a significant environmental threat due to its toxicity and persistence in soil and water. Our work aimed to evaluate the acute (survival) and chronic (regeneration, locomotion, and reproduction) toxicity of chlorpyrifos to the non-target freshwater planarian Girardia tigrina. The 48 h lethal concentration (LC50) of the commercial formulation, containing 480 g L-1 of chlorpyrifos, the active ingredient, was determined to be 622.8 µg a.i. L-1 for planarians. Sublethal effects were translated into a significant reduction in locomotion and delayed head regeneration (lowest observed effect concentration-LOEC = 3.88 µg a.i. L-1). Additionally, chlorpyrifos exposure did not affect planarian fecundity or fertility. Overall, this study demonstrates the potential of chlorpyrifos-based insecticides to harm natural populations of freshwater planarians at environmentally relevant concentrations. The observed toxicity emphasizes the need for stricter regulations and careful management of chlorpyrifos usage to mitigate its deleterious effects on aquatic ecosystems. By understanding the specific impacts on non-target organisms like G. tigrina, we can make more informed suggestions regarding the usage and regulation of organophosphate insecticides, ultimately promoting sustainable agricultural practices and environmental conservation.
RESUMO
Seasonal changes in climate and plant diversity are known to affect the population dynamics of both pests and natural enemies within agroecosystems. In Brazil, spontaneous plants are usually tolerated in small-scale physic nut plantations over the year, which in turn may mediate interactions between pests and natural enemies within this agroecosystem. Here, we aimed to access the influence of seasonal variation of abiotic (temperature, relative humidity and rainfall) and biotic (diversity of spontaneous plants, overall richness and density of mites) factors on the communities of phytophagous and predatory mites found in a physic nut plantation and its associated spontaneous plants. Mite sampling was monthly conducted in dicotyledonous and monocotyledonous leaves of spontaneous plants as well as in physic nut shrubs over an entire year. In the dry season there was a higher abundance of phytophagous mites (Tenuipalpidae, Tarsonemidae and Tetranychidae) on spontaneous plants than on physic nut shrubs, while predatory mites (Phytoseiidae) showed the opposite pattern. The overall density of mites on spontaneous plants increased with relative humidity and diversity of spontaneous plants. Rainfall was the variable that most influenced the density of mites inhabiting physic nut shrubs. Agroecosystems comprising spontaneous plants associated with crops harbour a rich mite community including species of different trophic levels which potentially benefit natural pest control due to increased diversity and abundance of natural enemies.
Assuntos
Ácaros/fisiologia , Controle Biológico de Vetores , Animais , Biodiversidade , Brasil , Cadeia Alimentar , Umidade , Modelos Lineares , Dinâmica Populacional , Comportamento Predatório , Chuva , Temperatura , Fatores de TempoRESUMO
The cassava green mite, Mononychellus tanajoa, is a key pest of cassava, Manihot esculenta Crantz (Euphorbiaceae), and it may be kept in check by naturally occurring predatory mites of the family Phytoseiidae. In addition to predatory mites, abiotic factors may also contribute to regulate pest mite populations in the field. Here, we evaluated the population densities of both M. tanajoa and the generalist predatory mite Euseius ho DeLeon (Acari: Phytoseiidae) over the cultivation cycle (11 months) of cassava in four study sites located around the city of Miranda do Norte, Maranhão, Brazil. The abiotic variables rainfall, temperature and relative humidity were also recorded throughout the cultivation cycle of cassava. We determined the relative importance of biotic (density of E. ho) and abiotic (rainfall, temperature and relative humidity) factors to the density of M. tanajoa. The density of M. tanajoa increased whereas the density of E. ho remained constant throughout time. A hierarchical partitioning analysis revealed that most of the variance for the density of M. tanajoa was explained by rainfall and relative humidity followed by E. ho density and temperature. We conclude that abiotic factors, especially rainfall, were the main mechanisms driving M. tanajoa densities.
Assuntos
Tetranychidae/fisiologia , Animais , Brasil , Umidade , Manihot , Controle Biológico de Vetores , Densidade Demográfica , Dinâmica Populacional , Chuva , TemperaturaRESUMO
Freshwater benthic environments are among the major sinks of microplastics (MPs, < 5 mm) sourced on inland anthropogenic activities. The ecotoxicological effects of MPs on benthic macroinvertebrates have been assessed preferably in collectors, shredders, and filter-feeders, but resulting in insufficient knowledge on the potential trophic transfer and its effects on macroinvertebrates with predator behaviour such as planarians. This work evaluated the behavioural (feeding, locomotion), physiological (regeneration) and biochemical responses (aerobic metabolism, energy reserves, oxidative damage) of the planarian Girardia tigrina after consuming contaminated live prey Chironomus riparius larvae previously exposed to microplastics of polyurethane (PU-MPs; 7-9 µm in size; 375 mg PU-MPs/kg). After the feeding period (3 h), planarians consumed 20 % more contaminated prey than uncontaminated prey, probably related to increased curling/uncurling movements of larvae (that might be more appellative to planarians). Histological analysis revealed planarians' limited intake of PU-MPs, mainly detected near the pharynx. The consumption of contaminated prey (and intake of PU-MPs) did not result in oxidative damage but slightly increased the aerobic metabolism and energy reserves which show that the consumption of more prey was sufficient to cope with the potential adverse effects of internalized MPs. Moreover, no effects were observed in the locomotion of planarians in good agreement with the hypothesis of sufficient energy acquired by the exposed planarians. Despite the previous, it seems that the energy acquired was not allocated for planarians' regeneration since a significant delay in the regeneration of the auricles was observed for planarians feeding on contaminated prey. Therefore, further studies should be performed considering the potential long-term effects (i.e., reproduction/fitness) and the effects of MPs that might result from continuous feeding on contaminated prey, representing a more realistic exposure scenario.
Assuntos
Planárias , Poluentes Químicos da Água , Animais , Microplásticos , Plásticos , Planárias/fisiologia , Poluentes Químicos da Água/toxicidade , Água DoceRESUMO
BACKGROUND: The significance of morphological responses of hosts on susceptibility against gall-inducing insects is relatively unknown, especially in planted forests. Here, we investigate the temporal morphological responses (gall development) induced by the invasive gall wasp Leptocybe invasa and the subsequent insect development in two Eucalyptus clones. RESULTS: Our results identified a novel stage of gall development, not previously reported, termed here 'brownish ring'. In both hosts similar gall development stages were observed. Although L. invasa oviposited in both clones, comparison of external morphological traits of galls over time revealed a differential response in the number of galls between clones. Comparison of the developmental time of each gall and insect stage between clones suggests that plant defense mechanisms against L. invasa are activated shortly after oviposition by the wasp, yet before gall formation. CONCLUSION: Gall number is an important parameter that should be used to measure host susceptibility among Eucalyptus clones. To the best of our knowledge, this is the first study showing differential morphological responses induced by a galling insect, even before gall formation, revealing differences in susceptibility between different plant hosts. These findings provide insight into the use of early stages of gall formation by L. invasa to prevent invasion and establishment of this pest.
Assuntos
Eucalyptus , Vespas , Animais , Células Clonais , Feminino , Interações Hospedeiro-Parasita , Oviposição , Tumores de PlantaRESUMO
Microbial insecticides are being used as ecologically-friendly alternatives to traditional insecticides. However, their effects have been poorly investigated on non-target freshwater species, with exception of a few insect species. Moreover, combined effects of microbial insecticides with other environmental stressors, such as salinity, have never been investigated. Thus, our goal was to assess the effects of Bac-Control® (based in Bacillus thuringiensis - Btk) and Boveril® (based in Beauveria bassiana - Bb) with increasing salinities (NaCl) on freshwater planarian Girardia tigrina. It has been reported that increased salinity levels affect freshwater organisms compromising their survival by triggering adaptation processes to cope with osmotic stress. Our results showed delayed regeneration, decreased locomotion and feeding on planarians exposed to NaCl, whereas their sexual reproduction was not affected. Both microbial insecticides impaired feeding, locomotor activity, regeneration, and sexual reproduction of planarians. Planarians exposed to microbial insecticides compromised their progeny. Therefore, microbial insecticides might not be ecologically friendly alternatives to chemical insecticides. Interestingly, harmful effects of microbial insecticides with increasing salinities showed an inadequate response of planarians to cope with induction of their immune response and osmoregulation.
Assuntos
Planárias , Poluentes Químicos da Água , Animais , Agentes de Controle Biológico , Água Doce , Reprodução , Cloreto de Sódio , Poluentes Químicos da Água/toxicidadeRESUMO
Frankliniella schultzei (Trybom) is a serious pest of melon crops and is commonly found in the main producing areas of melon in Brazil (North and Northeast regions). This pest causes significant losses, damaging plants through feeding and tospovirus vectoring. Thus, the proper management of F. schultzei is crucial to prevent economic losses, and knowledge of the within-field distribution patterns of F. schultzei can be used to improve this pest management. This study aimed to determine the within-field distribution (through semivariogram modeling and kriging interpolation) and the factors associated with F. schultzei abundance in open-field yellow melon crops. We surveyed four yellow melon fields located in Formoso do Araguaia (Tocantins state, North Brazil) for thrips abundance in various crop stages (vegetative, flowering, and fruiting) in 2015 and 2016. Twelve models were fitted and it was determined that F. schultzei counts were strongly aggregated. The median spatial dependence was 4.79 m (range 3.55 to 8.02 m). The surface maps generated by kriging depicted an edge effect in fields 3 and 4. In addition, correlation analyses indicated that air temperature and presence of surrounding cucurbits are positively associated with F. schultzei abundance in yellow melon fields. Altogether, these insights can be combined for spatially based pest management, especially when the conditions (cucurbits in the surroundings and warmer periods) are favorable to F. schultzei.
Assuntos
Cucurbitaceae , Tisanópteros , Tospovirus , Animais , Brasil , Controle de PragasRESUMO
Paraquat (PQ) is still used in several countries worldwide as an herbicide for weed control in agricultural production, ponds, reservoirs and irrigation canals. Thus, PQ is frequently found in surface water systems and is potentially toxic to aquatic organisms, since it can cause mitochondrial dysfunction altering in the redox state of cells. This study aimed to investigate the chronic effects of PQ to Chironomus riparius and Girardia tigrina, and compare their physiological strategies to cope with environmental stress. The mean emergence time was the most sensitive endpoint for Chironomids, with the lowest observed effect concentrations (LOEC) being 0.02 for males and 0.1 mg PQ L-1 for females. Moreover, PQ reduced the body weight of male and female imagoes, with LOECs of 0.5 and 2.5 mg PQ L-1, respectively. Paraquat also decreased the respiration rate (LOEC = 2.5 mg PQ L-1) and total glutathione (tGSH) content (LOEC = 0.5 mg PQ L-1). Thus, the aerobic production of energy was not affected and allowed chironomids to cope with oxidative stress induced by PQ, but with consequent physiological costs in terms of development rates and weight of adults. In planarians, PQ decreased the locomotion and feeding activity, and delayed photoreceptor regeneration (LOECs = 2.5 mg PQ L-1 for all endpoints). Despite increased aerobic energy production (LOEC = 0.5 mg PQ L-1), planarians were not able to cope with oxidative stress induced by the highest PQ concentrations, since lipid peroxidation levels were significantly increased (LOEC = 2.5 mg PQ L-1) concomitantly with a significant decrease of tGSH (LOEC = 2.5 mg PQ L-1). These results showed that planarians were unable to cope with oxidative stress induced by PQ with consequent impairments of behavior and regeneration despite an increased aerobic energy production.
Assuntos
Chironomidae , Herbicidas , Planárias , Animais , Feminino , Masculino , Estresse Oxidativo , ParaquatRESUMO
BACKGROUND: Decision-making systems are essential parts of integrated pest management programs. The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is a major pest of many crops, including watermelon (Citrullus lanatus), the second most consumed fruit worldwide. However, there are still no studies on decision-making systems for B. tabaci on this crop. Thus, we aim to determine a decision-making system to control B. tabaci biotype B on watermelon crops. RESULTS: The highest densities of B. tabaci biotype B reduced the yield of watermelon crops by up to 56%. Watermelon plants were more susceptible to the pest at the vegetative stage than at the reproductive stage. The economic injury levels of B. tabaci biotype B when watermelon prices were low, average, and high were respectively, 0.52, 0.21 and 0.13 adults leaf-1 at the vegetative stage, and 1.69, 0.69 and 0.44 adults leaf-1 at the reproductive stage. The sequential plans reached the same decisions as the conventional plan, and reduced the time and cost of sampling by up to 92.68%. CONCLUSION: The decision-making systems determined in this study enable an expedited and proper decision to be made for controlling B. tabaci, biotype B and can be used in different price situations for watermelon and in crops at different phenological stages. © 2018 Society of Chemical Industry.
Assuntos
Citrullus , Técnicas de Apoio para a Decisão , Hemípteros , Controle de Insetos/métodos , Animais , Brasil , Citrullus/crescimento & desenvolvimentoRESUMO
The ecological and economic contributions of pollinator bees to agricultural production have been threatened by the inappropriate and excessive use of pesticides. These pesticides are often applied in areas with ecological peculiarities (e.g., the Neotropical savannah-like region termed as Cerrado) that were not considered during the product development. Here, we conducted field experiments with melon (i.e., Cucumis melo L.) plants cultivated under Brazilian Cerrado conditions and evaluated the impacts of botanical (i.e., neem-based insecticide) and synthetic (i.e., the pyrethroid insecticide deltamethrin and the fungicides thiophanate-methyl and chlorothalonil) pesticides on the flower visitation rates of naturally occurring pollinator bees. Our results revealed that both honey bees (i.e., Apis mellifera L.) and non-Apis bees visited melon flowers and the intensity of bee visitation was moderately correlated with yield parameters (e.g., number of marketable fruits and fruit yield). Pesticide treatments differentially affected bee species. For instance, Plebeia sp. bees were not affected by any pesticide treatment, whereas both A. mellifera and Halictus sp. bees showed reduced visitation intensity after the application of deltamethrin or neem-based insecticides. Fungicide treatment alone did not influence the bee's visitation intensity. Deltamethrin-treated melon fields produced significantly lighter marketable fruits, and the melon yield was significantly lower in melon fields treated with the neem-based insecticide. Thus, our findings with such pollinator bees reinforce the idea that field applications of botanical pesticides may represent as risky as the applications of synthetic compounds, indicating that these alternative products should be submitted to risk assessments comparable to those required for synthetic products.
Assuntos
Abelhas/efeitos dos fármacos , Comportamento Animal/efeitos dos fármacos , Cucurbitaceae/crescimento & desenvolvimento , Flores/crescimento & desenvolvimento , Inseticidas/toxicidade , Polinização , Animais , Abelhas/fisiologia , Brasil , Glicerídeos/toxicidade , Polinização/efeitos dos fármacos , Piretrinas/toxicidade , Terpenos/toxicidadeRESUMO
The fungicide cyproconazole (CPZ) inhibits the biosynthesis of ergosterol, an essential sterol component in fungal cell membrane and can also affect non-target organisms by its inhibitory effects on P450 monooxygenases. The predicted environmental concentration of CPZ is up to 49.05 µg/L and 145.89 µg/kg in surface waters and sediments, respectively, and information about CPZ toxicity towards non-target aquatic organisms is still limited. This study aimed to address the lack of ecotoxicological data for CPZ, and thus, an evaluation of the lethal and sub-lethal effects of CPZ was performed using two freshwater invertebrates (the midge Chironomus riparius and the planarian Dugesia tigrina). The estimated CPZ 48 h LC50 (95% CI) was 17.46 mg/L for C. riparius and 47.38 mg/L for D. tigrina. The emergence time (EmT50) of C. riparius was delayed by CPZ exposure from 0.76 mg/L. On the other hand, planarians showed higher tolerance to CPZ exposure. Sub-lethal effects of CPZ on planarians included reductions in locomotion (1.8 mg/L), delayed photoreceptors regeneration (from 0.45 mg/L), and feeding inhibition (5.6 mg/L). Our results confirm the moderate toxicity of CPZ towards aquatic invertebrates but sub-lethal effects observed also suggest potential chronic effects of CPZ with consequences for population dynamics.
Assuntos
Organismos Aquáticos/efeitos dos fármacos , Água Doce/química , Planárias/efeitos dos fármacos , Triazóis/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Chironomidae/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ecotoxicologia , Dose Letal MedianaRESUMO
Spatiotemporal dynamics studies of crop pests enable the determination of the colonization pattern and dispersion of these insects in the landscape. Geostatistics is an efficient tool for these studies: to determine the spatial distribution pattern of the pest in the crops and to make maps that represent this situation. Analysis of these maps across the development of plants can be used as a tool in precision agriculture programs. Watermelon, Citrullus lanatus (Thunb.) Matsum. and Nakai (Cucurbitales: Cucurbitaceae), is the second most consumed fruit in the world, and the whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is one of the most important pests of this crop. Thus, the objective of this work was to determine the spatiotemporal distribution of B. tabaci in commercial watermelon crops using geostatistics. For 2 yr, we monitored adult whitefly densities in eight watermelon crops in a tropical climate region. The location of the samples and other crops in the landscape was georeferenced. Experimental data were submitted to geostatistical analysis. The colonization of B. tabaci had two patterns. In the first, the colonization started at the outermost parts of the crop. In the second, the insects occupied the whole area of the crop since the beginning of cultivation. The maximum distance between sites of watermelon crops in which spatial dependence of B. tabaci densities was observed was 19.69 m. The adult B. tabaci densities in the eight watermelon fields were positively correlated with rainfall and relative humidity, whereas wind speed negatively affected whiteflies population.
Assuntos
Citrullus , Cucurbitaceae , Hemípteros , Agricultura , Animais , Produtos AgrícolasRESUMO
BACKGROUND: The thrips Frankliniella schultzei is an important watermelon pest. Nevertheless, economic injury levels and sampling plans for this pest have not yet been determined for this crop. Thus, the objective of the present study was to determine the economic injury levels and develop sequential sampling plans for F. schultzei in conditions of low, medium and high fruit prices. RESULTS: The attack of F. schultzei on watermelon plants at the vegetative stage reduced the crop's productivity, which did not happen at the flowering and fruiting stage. The economic injury levels were 0.09, 0.04 and 0.02 thrips leaf-1 when the watermelon price was low ($US 62.5 t-1 ), medium ($US 140.63 t-1 ) and high ($US 218.75 t-1 ) respectively. The three sequential sampling plans for F. schultzei generated for the economic injury levels resulted in similar and more rapid decisions compared with the conventional plan, especially when the pest density was high. CONCLUSIONS: The three economic injury levels and the sequential sampling plans generated in the present study can be incorporated into integrated pest management programmes for watermelon crops because these plans provide a rapid and adequate control decision for F. schultzei. © 2016 Society of Chemical Industry.