RESUMO
BACKGROUND: The COVID-19 pandemic has significantly impacted education systems worldwide, with Brazil being one of the countries with the longest school closures. Over a million children and teenagers have been affected, leading to increased hunger and nutritional deficiencies. This study aimed to implement long-term surveillance of SARS-CoV-2 infections in public and private schools in Campo Grande, Brazil, after returning to in-person classes. METHODS: The study involved testing and genomic surveillance at 23 public and private schools in Campo Grande, Mato Grosso do Sul, Brazil, from October 18, 2021 to November 21, 2022. The participants eligible for enrollment were students aged 6-17 years and staff members from school institutions. At the time of collection, participants were asked if they had symptoms in the last two weeks. Whole-genome sequencing of SARS-CoV-2 was conducted to identify circulating variants and to compare them with those detected in the municipality. The demographic data and clinical history of the participants were described, and a logistic regression model was used to understand how the RT-qPCR results could be related to different characteristics. RESULTS: The study included 999 participants, most of whom were women. A total of 85 tests were positive, with an overall positivity rate of 3.2%. The dynamics of case frequency were consistent with those observed in the municipality during the study period. The most common symptoms reported were cough, rhinorrhea, headache, and sore throat. Symptoms were significantly associated with SARS-CoV-2 infection. Eleven lineages were identified in school community samples, with a frequency of occurrence per period similar to that found in the sequences available for the municipality. The most prevalent lineages within the sampling period were BA.2 (59.3%) and BA.5 (29.6%). CONCLUSIONS: Our findings demonstrate that schools can play a crucial role in epidemiological surveillance, helping trigger rapid responses to pathogens such as SARS-CoV-2. Long-term surveillance can be used to track outbreaks and assess the role of children and adults in transmission. It can also contribute to pandemic preparedness, enabling a rapid response to emergencies, such as COVID-19.
Assuntos
COVID-19 , SARS-CoV-2 , Instituições Acadêmicas , Humanos , COVID-19/epidemiologia , COVID-19/diagnóstico , Brasil/epidemiologia , Adolescente , Criança , Masculino , Feminino , Sequenciamento Completo do GenomaRESUMO
Introduction: With the reopening of schools during the coronavirus disease 2019 (COVID-19) pandemic, it was imperative to understand the role of students and education professionals in the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this paper, we determined the seroprevalence of the SARS-CoV-2 anti-nucleocapsid antibodies in the school community in Campo Grande, the capital and most populous city of the state of Mato Grosso do Sul (Brazil) and evaluated its association with sex, school level, and school type. Materials and methods: The survey was carried out in 20 public and private schools in the urban region of Campo Grande using the TR DPP® COVID-19 immunoglobulin M/immunoglobulin G (IgM/IgG) kit from the Immunobiological Technology Institute (Bio-Manguinhos, Rio de Janeiro, Brazil). Testing was carried out in three periods: from October to December 2021; from March to July 2022; and from August to November 2022. The participants were students aged 6-17 years enrolled in primary or secondary schools and professionals of different ages and roles. Results: During the first testing period, 162 participants were seropositive for the IgM and/or IgG anti-nucleocapsid SARS-CoV-2 antibodies, with an estimated seroprevalence of 19.6% using Bayesian multilevel regression. In the second period, 251 participants were seropositive (estimated seroprevalence, 34.6%), while in the third period, 393 participants were seroconverted (estimated seroprevalence, 56.7%). In 2022, there was an increase in the seroconversion rate compared to that in 2021. The most frequently described acute manifestations in the three periods were fever, headache, sore throat, and runny nose. In terms of the demographic profile, there was no predominance of seropositivity between the sexes, although women represented approximately 70% of the study population. There were also no differences between students and school staff. Discussion: The results made it possible to evaluate the extent of SARS-CoV-2 transmission in the school community through immunity developed against the virus, in addition to providing information about COVID-19 symptoms in children, adolescents, and adults.
Assuntos
COVID-19 , SARS-CoV-2 , Adolescente , Adulto , Criança , Humanos , Feminino , Brasil/epidemiologia , COVID-19/epidemiologia , Teorema de Bayes , Estudos Soroepidemiológicos , Anticorpos Antivirais , Imunoglobulina G , Imunoglobulina MRESUMO
The association between paracoccidioidomycosis (PCM) and AIDS is relatively rare in contrast to the higher incidence of other systemic mycosis. The explanation may be that AIDS is still predominantly an urban disease, and the PCM is endemic in Latin American rural areas. The aim of this study was to detect the prevalence of Paracoccidioides brasiliensis infection in HIV-positive patients at an endemic area of paracoccidioidomycosis in Brazil. Skin test with purified 43 kD glycoprotein (gp43) was performed in 90 HIV/AIDS patients. The prevalence found was 12.2% and it may be even greater, considering that HIV/AIDS patients may not respond to the intradermal test, which depends on cellular immunity for its positivity.
Assuntos
Síndrome da Imunodeficiência Adquirida/complicações , Paracoccidioides/isolamento & purificação , Paracoccidioidomicose/epidemiologia , Adulto , Brasil/epidemiologia , Estudos Transversais , Doenças Endêmicas , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Paracoccidioides/genética , Paracoccidioidomicose/etiologia , Paracoccidioidomicose/microbiologia , Prevalência , Adulto JovemRESUMO
Objetivo: identificar a qualidade e confiabilidade das informações sobre a pressão arterial invasiva abordadas em vídeos do YouTube. Método: pesquisa descritiva e exploratória com vídeos do YouTube sobre pressão arterial invasiva, identificados em busca única no dia 23 de fevereiro de 2023. Os vídeos foram organizados em uma lista de reprodução e submetidos à análise utilizando a ferramenta Discern Questionnaire para a qualidade educacional do vídeo e a Journal of American Medical Association Benchmarks para a confiabilidade e qualidade da informação e realizada análise estatística descritiva simples. Resultados: elencou-se 62 vídeos publicados há dois anos, dos quais 93,55% apresentaram qualidade educacional dos vídeos ruim, 6,45% moderada, demonstrando 70,97% baixa confiabilidade das informações e 32,25% citaram informações incompatíveis com a ciência. Conclusão: os vídeos encontrados na plataforma do YouTube apresentaram lacunas relacionados ao conteúdo de pressão arterial invasiva demonstrando não ser uma fonte confiável de respaldo para aprendizado do enfermeiro.
Objective: to identify the quality and reliability of information on invasive blood pressure covered in YouTube videos. Method: descriptive and exploratory research with YouTube videos on invasive blood pressure, identified in a single search on February 23, 2023. The videos were organized into a playlist and subjected to analysis using the Discern Questionnaire tool for the educational quality of the video and the Journal of American Medical Association Benchmarks for reliability and quality of information and performed simple descriptive statistical analysis. Results: 62 videos published two years ago were listed, of which 93.55% presented poor educational quality, 6.45% moderate, 70.97% demonstrated low reliability of information and 32.25% cited information incompatible with science. Conclusion: The videos found on the You Tube platform presented gaps related to the content of invasive blood pressure, demonstrating that it is not a reliable source of support for nurses' learning.
Objetivos:identificar la calidad y confiabilidad de la información sobre presión arterial invasiva cubierta en videos de YouTube. Método: investigación descriptiva y exploratoria con videos de YouTube sobre presión arterial invasiva, identificados en una única búsqueda el 23 de febrero de 2023. Los videos fueron organizados en una lista de reproducción y sometidos a análisis mediante la herramienta Discernir Cuestionario para la calidad educativa del video y la Journal of American Medical Association Se tomaron puntos de referencia para la confiabilidad y calidad de la información y se realizó un análisis estadístico descriptivo simple. Resultados: Se enumeraron 62 videos publicados hace dos años, de los cuales el 93,55% presentó mala calidad educativa, el 6,45% moderada, el 70,97% demostró baja confiabilidad de la información y el 32,25% citó información incompatible con la ciencia. Conclusión: Los videos encontrados en la plataforma You Tube presentaron lagunas relacionadas al contenido de presión arterial invasiva, demostrando que no es una fuente confiable de apoyo para el aprendizaje de los enfermeros.
Assuntos
Humanos , Masculino , Feminino , Rede Social , Educação não ProfissionalizanteRESUMO
Abstract Objective: To evaluate nurses' knowledge about the definitions of Sepsis-3 and updates to the Surviving Sepsis Campaign. Methods: This descriptive study was carried out from July to August 2018, with 30 nurses from four wards of a large university hospital. For data collection, we created, structured, and validated a questionnaire composed of socio-demographic/occupational data and knowledge test. Results: Only 16.6% of the professionals received in-service training on the subject. There was no implementation of sepsis protocols in the institution, although 96.6% of the participants considered their implementation necessary. Professionals aged ≥35 years old had a higher level of knowledge about the new definition of sepsis (p=0.042). The knowledge about volume resuscitation (p=0.001) and use of vasopressors (p=0.025) was greater in those with ≥10.5 years of experience in the profession. Nurses from the clinical units presented a higher level of knowledge about the organic dysfunctions caused by sepsis (p=0.025). Conclusion and implications for the practice: Nurses do not have satisfactory knowledge for the proper identification, treatment, and clinical management of sepsis. There is a need for greater professional, institutional, and political incentives to implement a permanent education and the sepsis protocol.
Resumen Objetivo: Evaluar el conocimiento de los enfermeros que actúan en enfermerías sobre las definiciones de Sepsis-3 y las actualizaciones de Surviving Sepsis Campaign. Métodos: Estudio descriptivo realizado de julio a agosto de 2018 con 30 enfermeros de cuatro enfermerías de un hospital universitario de gran porte. Para la recolección de datos, creamos, estructuramos y validamos una encuesta compuesta de datos sociodemográficos/ocupacionales y tests de conocimiento. Resultados: Solo el 16,6% de los profesionales recibieron capacitación en servicio sobre la temática. En la institución no había protocolo de sepsis implantado, aunque el 96,6% de los participantes consideraron su implantación necesaria. Los profesionales con edad ≥35 años presentaron mayor nivel de conocimiento acerca de la nueva definición de sepsis (p=0,042). El conocimiento sobre la resucitación volémica (p=0,001) y el uso de vasopresores (p=0,025) fue mayor en aquellos con tiempo ≥10,5 años de ejercicio en la profesión. Los enfermeros de las unidades clínicas presentaron un mayor nivel de conocimiento de las disfunciones orgánicas causadas por la sepsis (p=0,025). Conclusión e implicaciones para la práctica: Los enfermeros no presentan un conocimiento satisfactorio para identificar, tratar y administrar clinicamente la sepsis de forma adecuada. Existe la necesidad de mayores incentivos profesionales, institucionales y políticos, con miras a implementar una educación permanente y un protocolo de sepsis.
Resumo Objetivo: Avaliar o conhecimento dos enfermeiros que atuam em enfermarias sobre as definições do Sepsis-3 e atualizações da Surviving Sepsis Campaign. Métodos: Estudo descritivo realizado de julho a agosto de 2018 com 30 enfermeiros de quatro enfermarias de um hospital universitário de grande porte. Para coleta de dados, criamos, estruturamos e validamos um questionário composto por dados sociodemográficos/ocupacionais e teste de conhecimento. Resultados: Apenas 16,6% dos profissionais receberam treinamento em serviço sobre o tema. Na instituição não havia protocolo de sepse implantado, embora 96,6% dos participantes tenham considerado sua implantação necessária. Profissionais com idade ≥35 anos apresentaram maior nível de conhecimento acerca da nova definição de sepse (p=0,042). O conhecimento sobre ressuscitação volêmica (p=0,001) e uso de vasopressores (p=0,025) foi maior naqueles com tempo ≥10,5 anos de exercício na profissão. Enfermeiros das unidades clínicas apresentaram maior nível de conhecimento das disfunções orgânicas causada pela sepse (p=0,025). Conclusão e implicações para a prática: Os enfermeiros não apresentam conhecimento satisfatório para identificação, tratamento e gerenciamento clínico da sepse de forma adequada. Existe a necessidade de maiores incentivos profissionais, institucionais e políticos, com vistas às implementações da educação permanente e do protocolo de sepse.