Development of a monitoring system for disassembled towers with internal suspension poles.

The traditional construction monitoring methods of suspended pole-mounted decomposed towers are mostly manual monitoring. The monitoring personnel has multiple blind spots, and the possibility of misjudgment based on personal experience is relatively large. It is difficult to ensure the construction safety of the suspended pole decomposing tower. For this reason, combined with the current power Internet of Things technology, this paper develops an intelligent monitoring system for suspended pole-mounted decomposing towers. According to the construction technology and its safety requirements of inner suspension derrick for transmission tower erection in sections, this system is classified into intellisense layer, wireless transport layer and information integration layer. According to the physical characteristics of the seven major risk points of the inner suspension pole group tower, the intellisense layer developed corresponding sensing equipment to obtain risk information. In the wireless transport layer, the ZigBee and 4G communication technologies are selected to interconnect self-constituted LAN and 4G wide area networks, to complete on-site data interaction and long-distance transmission. In the information integration layer, the force of cable, the inclination and height of derrick, and the distance between derrick and tower are determined. The system has been verified by the 500 kV delivery project of Fujian Zhouning Pumped Storage Power Station. The average error of critical monitoring point data is 4.14%, and the average data transmission delays in the far and near fields of the system are 18 ms and 176 ms.

Three-dimensional porous composite scaffolds for in vitro marrow microenvironment simulation to screen leukemia drug.

The traditional 2D culture medium used for simulating the in vitro microenvironment for leukemia cells usually leads to 95% of the drug test results being different to the subsequent clinical results. Unlike this 2D culture, 3D scaffolds are more similar to the bone marrow microenvironment so can better simulate the drug effect on leukemia cells, which can benefit the preliminary screening of drugs for clinical use. For this purpose, the freeze-drying method was proposed for the fabrication of 3D scaffolds of graphene oxide/silk fibroin/carboxymethyl chitosan (GO/SF/CMCS). Experimental results show that these 3D scaffolds exhibit a better swelling ratio because of the embedding of GO. The improved hydrophilicity of the scaffolds brings about promoted adhesion and proliferation of leukemia cells. In contrast to the traditional 2D culture, leukemia cells in this 3D culture show stronger drug resistance, which is consistent with the previously reported clinical results. It implies that these 3D GO/SF/CMCS scaffolds can simulate well the in vivo bone marrow microenvironment, making it a promising platform for preliminary drug screening for clinical use.

[Effect of Polyvinyl Alcohol/Graphene Oxide Aerogel on the Biological Characteristics of Acute Leukemia Cell Lines].

OBJECTIVE: To investigate the effects of polyvinyl alcohol (PVA) + graphene oxide (GO, weight content 1 wt%) aerogel three-dimensional (3D) scaffolds culture system on the proliferation, phenotype and drug resistance of ALL cell line Jurkat and AML cell line HL-60. METHODS: Jurkat cells and HL-60 cells were seeded in PVA+GO aerogel scaffolds for culture, and the structure of cells were observed by the scanning electron microscopy. Cell proliferation activity was measured by Cell Counting Kit-8 (CCK-8), cell phenotypes were analyzed by flow cytometry after fluorescent staining, then were compared with 2D cultured cells. Ara-C was used in drug resistance experiment, and CCK8 was used to detected cell proliferation activity. RESULTS: The proliferation activity of Jurkat cells grown in aerogel scaffolds was higher than that by 2D cultured in long-term culture. However, in HL-60 cells, the proliferation activity on 3D scaffold only at the 8th to 20th day was higher than that on the traditional 2D culture. Expression of CD4 in Jurkat cells increased after culture for 30 days, but the cell phenotypes in the 3D aerogel scaffolds were similar to 2D cultured cells. Phenotype of HL-60 cells was certainly changed after culture for 30 days, the cells can be divided into CD13+CD14-CD45+HLA-DR+,CD13-CD14--CD45+HLA-DR+ and CD13-CD14-CD45+HLA-DR- groups, and a new CD13+CD14-CD45-HLA-DR+ group of cells appeared in the cells cultured in 3D scaffolds, but not in 2D cultured cells. Drug resistance experiments showed that Jurkat cells in aerogel scaffolds have stronger drug resistance than those in 2D culture. CONCLUSION: PVA+GO (1 wt%) aerogel scaffolds can improve the proliferation and drug resistance of leukemia cells, and the phenotypes were the same as those in 2D culture, which can be used for cell amplification and biology characteristics studies and drug experiments. However, cell phenotypes should be analyzed before culture, and the effects of phenotypes changes on drug resistance should be eliminated.