Early-life exposure to the Chinese famine and risk of hyperuricaemia in adult females in Qingdao.

Two population-based cross-sectional surveys involving randomly selected Chinese adults aged 35-74 years were conducted in Qingdao, China in 2006 and 2009. Nine thousand fifty-five subjects from the two surveys were grouped into four birth groups of fetal/infant exposed (born between 1 January 1959 and 31 December 1962), childhood exposed (born between 1 January 1950 and 31 December 1958), adolescence exposed (born between 1 January 1942 and 31 December 1949) and the unexposed (born before 1941 and after 1963). Multivariate logistic regression models were used to calculate the OR and 95 % CI of hyperuricaemia in different exposed groups. Overall, famine exposure in the fetal/infant period, childhood and adolescence was not associated with adulthood hyperuricaemia (all P > 0·05). In females, childhood exposed group (OR = 1·59, 95 % CI 1·25, 2·02) and adolescence exposed group (OR = 1·74, 95 % CI 1·30, 2·33) both had higher risks to have hyperuricaemia in adult. However, this difference was not found in fetal/infant exposed group. In males, no significant relation was observed in any famine exposed group (all P > 0·05). Exposure to famine in childhood and adolescence is associated with an increased risk of hyperuricaemia for adulthood of females, but not in males. Adequate nutrition during early life appears to be beneficial to prevent hyperuricaemia of adult females.

Individual and combined relationship of serum uric acid and alanine aminotransferase on metabolic syndrome in adults in Qingdao, China.

BACKGROUND AND AIMS: Associations of alanine aminotransferase (ALT) and serum uric acid (SUA) with metabolic syndrome (MetS) remain controversial. We aimed to explore individual and combined effects of ALT and SUA on MetS in community residents. METHODS AND RESULTS: A population-based cross-sectional survey involving randomly selected Chinese adults aged 35-74 years was conducted in 2009 in Qingdao, China, and 4642 participants were included in the current study. Based on a combination of SUA and ALT levels in the tertile, subjects were grouped into Group 1-9. The individual and combined relations of SUA and ALT to MetS were analyzed by logistic regression models. The prevalence of MetS was 28.50% in males and 22.30% in females. ALT and SUA were independently associated with MetS and ORs (95% CIs) were 1.55 (1.42-1.70) and 1.92 (1.72-2.14), respectively, after adjusting for potential confounders. With the elevation of ALT and SUA levels, the risk of developing MetS increased. Compared to Group 1, ORs (95% CIs) of combined ALT and SUA for MetS were 2.21 (1.70-2.88), 4.02 (3.10-5.21), 2.19 (1.62-2.97), 2.53 (1.91-3.34), 4.69 (3.60-6.12), 1.76 (1.17-2.64), 3.65 (2.63-5.06) and 7.15 (5.41-9.46) in Group 2-9, respectively. CONCLUSIONS: ALT and SUA were both related to MetS independently. Combined elevation of ALT and SUA levels could increase the risk of MetS and its components than an elevation in SUA and ALT alone. Therefore, measures should be taken to lower SUA and ALT levels to reduce the risk of having MetS.

Clinical outcomes of Preimplantation genetic testing (PGT) application in couples with chromosomal inversion, a study in the Chinese Han population.

BACKGROUND: Chromosomal inversion was considered to have adverse effects on pregnancy outcomes through abnormal gametogenesis. The purpose of this retrospective study was to investigate whether preimplantation genetic testing (PGT) improves pregnancy outcomes for couples with chromosomal inversion. METHODS: A total of 188 cycles from 165 couples with one chromosomal inversion carrier were divided into two groups: PGT (136 cycles, 125 couples) and non-PGT (52 cycles, 50 couples). Biochemical pregnancy, clinical pregnancy, ongoing pregnancy, miscarriage and live birth rates of their first transfer cycles, as well as cumulative live birth rates of each cycle and euploidy rates, were analyzed. RESULTS: There were no statistically significant differences in the pregnancy outcomes between the two groups. The euploidy rate of pericentric inversion carriers was not higher than that of paracentric inversion carriers in PGT group (60.71% vs 50.54%, P = 0.073). Similarly, the euploid rate of male carriers was not higher than that of female carriers (61.2% vs 56.1%, P = 0.256). CONCLUSIONS: Due to limitation of retrospective study and small sample size, our current data showed that PGT cannot provide prominent benefits for inversion carriers in the Chinese Han population. Further prospective randomized controlled trials are needed to evaluate the effects of PGT.

Assessing the Impact of Stem Cell-based Therapy on Periodontal Health: A Meta-analysis of Clinical Studies.

OBJECTIVE: While clinical trials exploring stem cells for regenerating periodontal tissues have demonstrated positive results, there is a limited availability of systematic literature reviews on this subject. To gain a more comprehensive understanding of stem cell interventions in periodontal regeneration, this meta-analysis is undertaken to assess the beneficial effects of stem cells in human periodontal regeneration. METHODS: "PubMed," "Cochrane Library," "Web of Science," "Embase," "Wanfang," and "CNKI," were used to extract clinical studies related to the utilization of stem cells in repairing periodontal tissue defects. This search included studies published up until October 5, 2023. The inclusion criteria required the studies to compare the efficacy of stem cell-based therapy with stem cell-free therapy for regenerating periodontal tissues. Meta-analysis was conducted using Review Manager software (version 5.4). RESULTS: This meta-analysis synthesized findings from 15 selected studies investigating the impact of stem cell interventions on periodontal tissue regeneration. The "stem cell" group displayed a substantial reduction in clinical attachment level (CAL) compared to the "control" group within 3 to 12 months post-surgery. However, no significant differences in CAL gain were found between groups. Probing pocket depth (PPD) significantly decreased in the "stem cell" group compared to the "control" group, particularly for follow-up periods exceeding 6 months, and dental stem cell treatment exhibited notable improvements. Conversely, no significant differences were observed in PPD reduction. Gingival recession (GR) significantly decreased in the "stem cell" group compared to the "control" group at 3 to 12 months post-surgery. No significant differences were observed in GR reduction between groups. No significant differences were identified in cementoenamel junction-bone distance reduction, infrabony defect reduction, or bone mineral density increase between the two groups. Furthermore, no significant changes were observed in the gingival index, plaque index, or width of keratinized gingiva. CONCLUSION: In conclusion, while stem cell-based therapy offers promising prospects for periodontal defect treatment, there are notable limitations in the current body of research. Larger, multicenter, double-blind RCTs with robust methodologies are needed to provide more reliable evidence for stem cell-based intervention in periodontitis.

Excessive Lipid Peroxidation in Uterine Epithelium Causes Implantation Failure and Pregnancy Loss.

The uterine epithelium undergoes a dramatic spatiotemporal transformation to enter a receptive state, involving a complex interaction between ovarian hormones and signals from stromal and epithelial cells. Redox homeostasis is critical for cellular physiological steady state; emerging evidence reveals that excessive lipid peroxides derail redox homeostasis, causing various diseases. However, the role of redox homeostasis in early pregnancy remains largely unknown. It is found that uterine deletion of Glutathione peroxidase 4 (GPX4), a key factor in repairing oxidative damage to lipids, confers defective implantation, leading to infertility. To further pinpoint Gpx4's role in different cell types, uterine epithelial-specific Gpx4 is deleted by a lactotransferrin (Ltf)-Cre driver; the resultant females are infertile, suggesting increased lipid peroxidation levels in uterine epithelium compromises receptivity and implantation. Lipid peroxidation inhibitor administration failed to rescue implantation due to carbonylation of major receptive-related proteins underlying high lipid reactive oxygen species. Intriguingly, superimposition of Acyl-CoA synthetase long-chain family member 4 (ACSL4), an enzyme that promotes biosynthesis of phospholipid hydroperoxides, along with uterine epithelial GPX4 deletion, preserves reproductive capacity. This study reveals the pernicious impact of unbalanced redox signaling on embryo implantation and suggests the obliteration of lipid peroxides as a possible therapeutic approach to prevent implantation defects.

Combined miRNA and mRNA sequencing reveals the defensive strategies of resistant YHY15 rice against differentially virulent brown planthoppers.

Introduction: The brown planthopper (BPH) poses a significant threat to rice production in Asia. The use of resistant rice varieties has been effective in managing this pest. However, the adaptability of BPH to resistant rice varieties has led to the emergence of virulent populations, such as biotype Y BPH. YHY15 rice, which carries the BPH resistance gene Bph15, exhibits notable resistance to biotype 1 BPH but is susceptible to biotype Y BPH. Limited information exists regarding how resistant rice plants defend against BPH populations with varying levels of virulence. Methods: In this study, we integrated miRNA and mRNA expression profiling analyses to study the differential responses of YHY15 rice to both avirulent (biotype 1) and virulent (biotype Y) BPH. Results: YHY15 rice demonstrated a rapid response to biotype Y BPH infestation, with significant transcriptional changes occurring within 6 hours. The biotype Y-responsive genes were notably enriched in photosynthetic processes. Accordingly, biotype Y BPH infestation induced more intense transcriptional responses, affecting miRNA expression, defenserelated metabolic pathways, phytohormone signaling, and multiple transcription factors. Additionally, callose deposition was enhanced in biotype Y BPH-infested rice seedlings. Discussion: These findings provide comprehensive insights into the defense mechanisms of resistant rice plants against virulent BPH, and may potentially guide the development of insect-resistant rice varieties.

Developing a Pocket Park Prescription Program for Human Restoration: An Approach That Encourages Both People and the Environment.

Healing through nature has long been confirmed as an efficient way to improve human physical and psychological health in contemporary urban life. This concept evolved into the well-known Park Prescription Program. However, the psychological restoration imparted by nature was not particularly emphasized in the original Park Prescription Program; it primarily addresses the regulation of physical activities. The quality of urban parks may affect how well people pursue these prescriptions, but the program rarely includes designers among its stakeholders. This study is inspired by the Park Prescription Program, and its intent is to develop a Pocket Park Prescription Program that encourages usage by active people and proper landscape design. The inclusion of designers has been found to be extremely important for pocket parks since they are limited in size but have the advantages of high flexibility and accessibility, and their restorative potential needs be maximized with the limited resources available. Ten pocket parks with distinct functional and landscape attributes were selected in Shanghai as research sites. The Restorative Component Scale was designed into a questionnaire-based survey to measure how people perceived restorative experiences in each site. The onsite survey also incorporated questions investigating people's behaviour characteristics of using these pocket parks. Site photos were taken and analysed with semantic image segmentation to indicate the landscape compositions of each site. The relations between people perceived restorativeness and parks' using patterns and landscape characteristics were then explored with correlation analysis to provide cues on instructing how people's visiting behavior and park landscape design can be improved. The results suggest there are better restoration results when people stay longer in pocket parks, and when people visit their neighborhood pocket parks two to three times a week, these benefits are further enhanced. This study also found that when these prescribed health behaviors are uncertain, the restorative experiences perceived by people can be improved with design interventions in regard to landscape elements such as vegetation, person, decorative lamps, pavement and terrain. These interventions should be made also in consideration of specific park functions. The research outcome intends to show that designers should be included as stakeholders in the Pocket Park Prescription Program, and it is expected to guide individuals towards effectively using pocket parks for restoration. This is to ensure that both the design and the people's perspectives will be strengthened through the implementation of this program.

The effects of the natural visual-aural attributes of urban green spaces on human behavior and emotional response.

Introduction: Nature-based solutions (NBS) have been used to address a wide range of urban environmental challenges, an important aspect of which is to improve human health and well-being. However, most relevant studies focus either on what positive influences nature may have or on identifying what natural factors can have these benefits. Few have investigated the sensory composition of nature and the effects of nature in different sensory aspects on human health. Setting out from the multi-sensory perspective, this study aims to explore human behavior and emotional response from visual and aural contact with urban nature. Methods: Taking Jiangjia Art Garden in Chengdu as an example, natural attributes such as its visual (landscape) and aural (sound source) characteristics as well as people's activities (behavioral responses) were measured by on-site mapping analysis. This was done while a questionnaire-based survey was conducted to investigate people's emotional responses regarding their overall satisfaction, pleasantness, calm, and agreeableness. Results: The results indicated that nature-dominated visual landscapes such as grassland, waterscapes, and woodlands, as well as natural sounds such as bird sounds, chirp sounds, and wind sounds were found to be positively correlated to the vitality of activities and people's emotional status. Regarding behavioral responses, it was shown that grasslands and woodlands are more likely to be attractive places for recreation, and the vitality measured became extremely high when these two were paired with lakes. As for the emotional responses, people's perceived overall satisfaction, calm, and agreeableness were equally reflected in their behavioral patterns, suggesting a strong relationship with natural factors. Discussion: The research findings were visually presented in behavior and emotional maps to provide direct cues of informing the future design of high-quality urban green spaces and promoting the application of aural-visual experience in the design of urban nature areas.

Individual and combined associations of alanine aminotransferase and hemoglobin with metabolic syndrome in the elderly in Qingdao, China.

Background and aims: Combined associations of alanine aminotransferase (ALT) and hemoglobin (Hb) with metabolic syndrome (MetS) have not been assessed yet. The current study investigated the independent and combined relationships between ALT, Hb, and MetS in the elderly. Methods: The 37,966 elderly participants aged 65 years and older were recruited from community centers in Qingdao, China. The sampled elderly population visited the health centers once a year where they were offered a free health checkup. Based on a combination of ALT and Hb levels categorized by tertile, participants were grouped into nine groups (Group 1-9). Logistic regression models were used to analyze the individual and combined associations of ALT and Hb with MetS. Results: ALT and Hb were both independently related to MetS in both genders. With the elevation of ALT or Hb levels, risks for MetS and its components increased. Compared to the reference group (the 1st tertiles of both ALT and Hb levels), respective odds ratio of combined ALT and Hb for MetS in Group 2-9 ranged from 1.32-3.38 and 1.14-2.31 in men and women after adjusting for age, sex, education, married status, current smoking, current drinking, physical activity, and diet habit. Conclusion: ALT and Hb were both independently related to MetS and its components. Combined ALT and Hb levels could increase risks of MetS and its components than an elevation in ALT or Hb alone.

Genome wide analysis revealed conserved domains involved in the effector discrimination of bacterial type VI secretion system.

Type VI secretion systems (T6SSs) deliver effectors into target cells. Besides structural and effector proteins, many other proteins, such as adaptors, co-effectors and accessory proteins, are involved in this process. MIX domains can assist in the delivery of T6SS effectors when encoded as a stand-alone gene or fused at the N-terminal of the effector. However, whether there are other conserved domains exhibiting similar encoding forms to MIX in T6SS remains obscure. Here, we scanned publicly available bacterial genomes and established a database which include 130,825 T6SS vgrG loci from 45,041 bacterial genomes. Based on this database, we revealed six domain families encoded within vgrG loci, which are either fused at the C-terminus of VgrG/N-terminus of T6SS toxin or encoded by an independent gene. Among them, DUF2345 was further validated and shown to be indispensable for the T6SS effector delivery and LysM was confirmed to assist the interaction between VgrG and the corresponding effector. Together, our results implied that these widely distributed domain families with similar genetic configurations may be required for the T6SS effector recruitment process.

A Study on the Characteristics of Children's Natural Activities in the Neighborhood and Their Influencing Factors: Evidence from Hangzhou, China.

In the process of urbanization, children are becoming increasingly estranged from nature. The phenomenon has received widespread attention in developed countries because of its many negative effects on children's health and pro-nature behaviors. However, the existing studies lack the exploration of this issue in developing countries, especially with regard to China. In order to understand the characteristics of neighborhood natural activities and their influencing factors among children in China, we conducted a systematic questionnaire survey of 900 children (10-12 years old from Hangzhou City, Zhejiang Province, Eastern China) about their natural activities in the neighborhood. Children were asked to provide basic information on their neighborhood natural activities situation (frequency, duration and location), nature connectedness and environmental knowledge. The results showed that the frequency of children's neighborhood natural activities in rural areas was less than that of children in urban areas, but the naturalness of the neighborhood natural activity sites of rural children was higher than that of urban children. Boys spent more time engaging in neighborhood natural activities than girls. Only children outlasted non-only children in terms of the duration of neighborhood natural activities. Regarding Influencing Factors, children's nature connectedness and environmental knowledge significantly and positively predicted the frequency and location of children's natural activities in the neighborhood, which indicates a new approach to the promotion of children's neighborhood natural activities. Overall, how to encourage children to engage in natural activities in the neighborhood is a complex issue that needs to be studied in more regions and with more samples.

A High-Accuracy Detection System: Based on Transfer Learning for Apical Lesions on Periapical Radiograph.

Apical Lesions, one of the most common oral diseases, can be effectively detected in daily dental examinations by a periapical radiograph (PA). In the current popular endodontic treatment, most dentists spend a lot of time manually marking the lesion area. In order to reduce the burden on dentists, this paper proposes a convolutional neural network (CNN)-based regional analysis model for spical lesions for periapical radiographs. In this study, the database was provided by dentists with more than three years of practical experience, meeting the criteria for clinical practical application. The contributions of this work are (1) an advanced adaptive threshold preprocessing technique for image segmentation, which can achieve an accuracy rate of more than 96%; (2) a better and more intuitive apical lesions symptom enhancement technique; and (3) a model for apical lesions detection with an accuracy as high as 96.21%. Compared with existing state-of-the-art technology, the proposed model has improved the accuracy by more than 5%. The proposed model has successfully improved the automatic diagnosis of apical lesions. With the help of automation, dentists can focus more on technical and medical diagnoses, such as treatment, tooth cleaning, or medical communication. This proposal has been certified by the Institutional Review Board (IRB) with the certification number 202002030B0.

Benefits of Indirect Contact With Nature on the Physiopsychological Well-Being of Elderly People.

OBJECTIVES: Exposure to nature or to green space has positive mental health benefits. Closing of parks and green spaces during the COVID-19 pandemic has reduced options for mental health and well-being benefits and could have a greater impact on vulnerable populations, especially the elderly. The present study, therefore, explores the physiopsychological impacts of indirect contact with nature, using forest imagery, on the brain activity and autonomic nervous systems of elderly people. STUDY DESIGN: A within-subject design experiment was used. METHODS: Thirty-four participants aged 82.9 ± 0.78 years were asked to look at bamboo and urban images for 2 min. During the visual stimulation, α relative waves were measured using electroencephalography as an indicator of brain activity. Heart rate variability and skin conductance (SC) responses were utilized as indicators of arousal. Afterward, psychological responses were evaluated using the semantic differential and the Profile of Mood States questionnaires. RESULTS: Visual stimulation with bamboo image induced a significant increase in α relative waves and parasympathetic nervous activity and a significant decrease in SC. In addition, a significant increase in perceptions of "comfortable," "relaxed," "cheerful," and "vigorous" feelings was observed. CONCLUSIONS: Indirect contact with nature enhances the physiological and psychological conditions of the elderly. Findings can be used to guide the new design, renewal, and modification of the living environments of the elderly and those who are unable to get outside.

RNA-seq-Based Screening in Coal Dust-Treated Cells Identified PHLDB2 as a Novel Lung Cancer-Related Molecular Marker.

Lung cancer is one of the most serious leading cancers with high incidence globally. Identifying molecular markers is key for disease diagnosis and treatment. Coal dust might be important triggering factors in disease development. Here, we first performed RNA-seq-based screening in coal dust treated and nontreated RAW264.7 cell lines. PHLDB2 was found to be the top differentially expressed gene. By retrieving TCGA lung cancer dataset, we observed that PHLDB2 showed upregulations in males and smoker patients. Patients with lower PHLDB2 expression survived longer than those with higher expressions. Furthermore, PHLDB2 was negatively correlated with EMT makers, and a total of 2.74% mutation rate were observed in 1,059 patients. This finding highlights the critical role of PHLDB2 in lung cancer development. PHLDB2 might be a molecular maker for disease diagnosis or treatment.

Horticultural Activity: Its Contribution to Stress Recovery and Wellbeing for Children.

In recent years, children's use of mobile phones has grown rapidly, which might lead to an increase in mental stress and negatively affect their health. Despite increasing evidence that horticultural activity can provide significant health benefits, few scientific evidence-based studies are currently available regarding these benefits to children's health and wellbeing in schools. Therefore, this study aims to determine the potential benefits of horticultural activity for children from both psychological and physiological perspectives. Twenty-six elementary school students (mean age, 8.12 ± 0.21 years) were asked to perform a plant-related task and a mobile game task for 5 min. During both tasks, physiological sensors were used to measure the participants' heart rate variability, skin conductance, and skin temperature. Additionally, the participants' emotional responses were assessed using semantic differential and State-Trait Anxiety Inventory tests immediately after each task. Results revealed that, compared with the mobile game task, participants' health statuses were positively correlated with the horticultural task, including a considerable decrease in skin conductance and sympathetic nervous activity, together with a marginal increase in parasympathetic nervous activity. Such responses suggested that horticultural activity increased relaxation and decreased feelings of stress. Furthermore, the horticultural activity was associated with a substantial increment in comfort, naturalness, relaxation, and cheerfulness feelings, as well as a significant reduction in depression and a reduction in total anxiety levels. Given these positive benefits, horticultural activity may provide a great contribution to children's healthy life at school, prompt psychological relaxation and minimize mental stress relative to smartphone games.

Methylation in pericytes after acute injury promotes chronic kidney disease.

The origin and fate of renal myofibroblasts is not clear after acute kidney injury (AKI). Here, we demonstrate that myofibroblasts were activated from quiescent pericytes (qPericytes) and the cell numbers increased after ischemia/reperfusion injury-induced AKI (IRI-AKI). Myofibroblasts underwent apoptosis during renal recovery but one-fifth of them survived in the recovered kidneys on day 28 after IRI-AKI and their cell numbers increased again after day 56. Microarray data showed the distinctive gene expression patterns of qPericytes, activated pericytes (aPericytes, myofibroblasts), and inactivated pericytes (iPericytes) isolated from kidneys before, on day 7, and on day 28 after IRI-AKI. Hypermethylation of the Acta2 repressor Ybx2 during IRI-AKI resulted in epigenetic modification of iPericytes to promote the transition to chronic kidney disease (CKD) and aggravated fibrogenesis induced by a second AKI induced by adenine. Mechanistically, transforming growth factor-ß1 decreased the binding of YBX2 to the promoter of Acta2 and induced Ybx2 hypermethylation, thereby increasing α-smooth muscle actin expression in aPericytes. Demethylation by 5-azacytidine recovered the microvascular stabilizing function of aPericytes, reversed the profibrotic property of iPericytes, prevented AKI-CKD transition, and attenuated fibrogenesis induced by a second adenine-AKI. In conclusion, intervention to erase hypermethylation of pericytes after AKI provides a strategy to stop the transition to CKD.

The Therapeutic Effectiveness of Delayed Fetal Spinal Cord Tissue Transplantation on Respiratory Function Following Mid-Cervical Spinal Cord Injury.

Respiratory impairment due to damage of the spinal respiratory motoneurons and interruption of the descending drives from brainstem premotor neurons to spinal respiratory motoneurons is the leading cause of morbidity and mortality following cervical spinal cord injury. The present study was designed to evaluate the therapeutic effectiveness of delayed transplantation of fetal spinal cord (FSC) tissue on respiratory function in rats with mid-cervical spinal cord injury. Embryonic day-14 rat FSC tissue was transplanted into a C4 spinal cord hemilesion cavity in adult male rats at 1 week postinjury. The histological results showed that FSC-derived grafts can survive, fill the lesion cavity, and differentiate into neurons and astrocytes at 8 weeks post-transplantation. Some FSC-derived graft neurons exhibited specific neurochemical markers of neurotransmitter (e.g., serotonin, noradrenalin, or acetylcholine). Moreover, a robust expression of glutamatergic and γ-aminobutyric acid-ergic fibers was observed within FSC-derived grafts. Retrograde tracing results indicated that there was a connection between FSC-derived grafts and host phrenic nucleus. Neurophysiological recording of the phrenic nerve demonstrated that phrenic burst amplitude ipsilateral to the lesion was significantly greater in injured animals that received FSC transplantation than in those that received buffer transplantation under high respiratory drives. These results suggest that delayed FSC transplantation may have the potential to repair the injured spinal cord and promote respiratory functional recovery after mid-cervical spinal cord injury.