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Nat Commun ; 12(1): 6564, 2021 11 12.
Artigo em Inglês | MEDLINE | ID: mdl-34772931

RESUMO

Hybrids between diverged lineages contain novel genetic combinations but an impaired meiosis often makes them evolutionary dead ends. Here, we explore to what extent an aborted meiosis followed by a return-to-growth (RTG) promotes recombination across a panel of 20 Saccharomyces cerevisiae and S. paradoxus diploid hybrids with different genomic structures and levels of sterility. Genome analyses of 275 clones reveal that RTG promotes recombination and generates extensive regions of loss-of-heterozygosity in sterile hybrids with either a defective meiosis or a heavily rearranged karyotype, whereas RTG recombination is reduced by high sequence divergence between parental subgenomes. The RTG recombination preferentially arises in regions with low local heterozygosity and near meiotic recombination hotspots. The loss-of-heterozygosity has a profound impact on sexual and asexual fitness, and enables genetic mapping of phenotypic differences in sterile lineages where linkage analysis would fail. We propose that RTG gives sterile yeast hybrids access to a natural route for genome recombination and adaptation.


Assuntos
Diploide , Hibridização Genética , Infertilidade/genética , Meiose , Saccharomyces cerevisiae/genética , Mapeamento Cromossômico , Evolução Molecular , Genoma Fúngico , Recombinação Homóloga , Fenótipo , Proteínas de Saccharomyces cerevisiae/metabolismo
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