Evolution of the hemagglutinin gene of H3N8 canine influenza virus in dogs.

With the widespread use of a recently developed canine influenza virus (CIV) H3N8 vaccine, continual molecular evaluation of circulating CIVs is necessary for monitoring antigenic drift. The aim of this project was to further describe the genetic evolution of CIV, as well as determine any genetic variation within potential antigenic regions that might result in antigenic drift. To this end, the hemagglutinin gene of 19 CIV isolates from dogs residing in Colorado, New York, and South Carolina humane shelters was sequenced and compared to CIV strains isolated during 2003-2012. Phylogenetic analysis suggests that CIV might be diverging into two geographically distinct lineages. Using a mixed-effects model for evolution and single likelihood ancestor counting methods, several amino acid sites were found to be undergoing selection pressure. Additionally, a total of six amino acid changes were observed in two possible antigenic sites for CIVs isolated from Colorado and New York humane shelters between 2009 and 2011. As CIV isolates might be diverging into geographically distinct lineages, further experiments are warranted to determine the extent of antigenic drift occurring within circulating CIV.

Determinants of Salary for Veterinarians Employed in the Field of Shelter Medicine in the United States.

Despite recent growth in the field of shelter medicine, there is a paucity of wage data available. Understanding determinants of salary is helpful for new graduates deciding on a career path as well as employees and employers with regard to budgeting, professional development, and negotiations. An anonymous online survey was distributed via commercial survey platform to shelter veterinarians. Salary was analyzed using multiple linear regression, Kruskall-Wallis equality-of-populations rank-test, and Conover-Iman pairwise comparison. Of the 219 unique respondents, 197 worked as shelter veterinarians, with 157 full-time and 40 part-time. Fifteen respondents worked in academia, and 7 respondents worked in shelter leadership. Full-time shelter veterinarians had a median income of $92,000 [$78,000-110,000], which is the same as the median salary reported for all veterinarians by the AVMA in 2018. Salary depended on years of shelter experience (P = .004), supervision of other veterinarians (P = .015), region (Southwest, P = .010, Mideast, P = .010, and Far West, P = .002) and size of the metropolitan area (>1 million, P = .001 and <250,000, P = .011). Part-time veterinarians had a median income of $60,000 [$44,000-84,000]. The only variable predictive of salary was hours worked, with part-time veterinarians paid a median of $48 [36-66] per hour. Academic veterinarians had a median income of $108,000 [90,000-120,000]. For academics, title (assistant professor, P <.0001 and professor, P = .001), PhD (P = .010), and master's (P= .001) predicted salary. Veterinarians working in leadership positions had a median income of $120,000 [110,000-198,000], and no variable was significant. Veterinarians employed in academia and leadership had a higher salary than veterinarians employed in the shelter. There are predictors of salary for shelter medicine veterinarians, which will help both potential employers and employees, determine reasonable salaries when budgeting, negotiating, or planning personal development.

Surveys of Salary, Benefits, and Job Responsibilities for Veterinarians Employed in the Field of Shelter Medicine in the United States Conducted in 2011 and 2018.

According to American Veterinary Medical Association statistics, veterinarians employed in the not-for-profit sector account for the smallest group by type of employment. However, this group has experienced the greatest amount of change, with an increase of 52% between 2008 and 2013 and 55% between 2013 and 2018. Despite this growth, there has been a paucity of information regarding the salary, benefits, hours, and job duties that come with a career in shelter medicine. An initial survey of shelter medicine salary, benefits, hours, and job duties was conducted in 2011, and a similar survey conducted in 2018. All responses were anonymous. Results from 2018 were compared to 2011 using 2 sample test of proportions (counts) or Mann-Whitney (Wilcoxon rank sum) test (medians). A total of 207 conforming responses were received in 2011, and 219 in 2018. Median salary for all full-time veterinarians in the field of shelter medicine was $75,000 (interquartile range [IQR], 65,000-85,000) in 2011 and $94,500 (IQR, 80,000-110,000) in 2018. Part-time shelter employed veterinarians in 2011 worked a median of 20 hours (IQR 7-28) for an hourly rate of $41 (IQR, 29-56) while they worked a median of 26 hours (IQR, 15-32) for an hourly rate of $48 (IQR, 36-66) in 2018. There was an increase in the provision of all categories of benefits and an increase in veterinarians employed in academia and leadership. Demographics were similar, with the exception of increases in the number of boarded specialists and veterinarians who had completed a residency. Veterinarians reporting that shelter medicine had been offered in their curriculum increased by 44%. Budgets for shelters were reported to be higher, while animal intake and daily count of animals in care was lower. Comparison between surveys of shelter medicine veterinarians in 2011 and 2018 showed a consistent increase in salary, benefits and resources. There were indicators that the field of shelter medicine has matured as a discipline, particularly with regard to the availability of shelter medicine education and increased number of boarded specialists.

Impact of the Timing of Spay-Neuter Related to Transport on Disease Rates in Relocated Dogs.

Companion animal relocation programs are an important method to address geographic and resource disparities in pet overpopulation through transport from areas with high homeless pet populations to areas with high adopter demand. Despite mitigation by following best practices, a potential risk of animal relocation is increased disease incidence related to infectious disease spread and the effects of stress during transport. Surgical sterilization may compound disease risk due to the impact of surgical stress on disease susceptibility and the potential for disease exposure from other patients. Our study aimed to provide information about disease and surgical complication incidence as relates to the timing of surgical sterilization in relocated dogs. A population of 431 dogs relocated to a shelter in Washington State was monitored for disease while at the destination shelter and immediately post-adoption. No increased disease incidence was identified for dogs altered within two weeks of transport at the destination shelter compared with those altered within two weeks prior to transport at the source shelter. Because of disparities addressed by relocation programs, surgical sterilization of relocated companion animals is typically best performed at the destination shelter. Our study indicates that disease incidence is not increased by spay-neuter at the destination shelter.

Prevalence of selected infectious organisms and comparison of two anatomic sampling sites in shelter cats with upper respiratory tract disease.

In order to describe the isolation rates of potential pathogens and to compare anatomic sampling site suitability, nasal and pharyngeal swabs were taken from cats with acute clinical upper respiratory disease in a humane society. DNA of feline herpesvirus-1 was amplified from 51 of 52 cats sampled, Mycoplasma species were cultured or detected by PCR in samples from 34 of 42 cats sampled for both culture and PCR, and Bordetella bronchiseptica was isolated from three of 59 cats sampled for aerobic culture. A single swab was positive for calicivirus and no swabs were positive for Chlamydophila felis. Mycoplasma, Pasteurella and Moraxella species were all isolated from at least one cat in which no primary pathogen was identified. With the exception of B. bronchiseptica, which was detected in nasal swabs only, recovery rates for all suspect primary pathogens were comparable between sampling sites.

Evaluation of pradofloxacin for the treatment of feline rhinitis.

Forty humane society cats with suspected bacterial upper respiratory infections (URIs) were studied in order to compare amoxycillin and pradofloxacin for treatment of rhinitis and describe common pathogens. Nasal discharges were collected prior to random placement into one of three treatment groups. Cats failing to initially respond were crossed to the alternate drug. Drug toxicity was not noted. The organisms most frequently isolated or amplified pre-treatment were feline herpesvirus-1 (75%), Mycoplasma species (62.5%), Bordetella species (47.5%), Staphylococcus species (12.5%) and Streptococcus species (10.0%). No differences in clinical scores between groups over time were noted. Overall response rates for amoxycillin at 22 mg/kg, q12 h for seven doses (10/15 cats; 67%), pradofloxacin at 5mg/kg, q24 h for seven doses (11/13 cats; 85%), and pradofloxacin at 10mg/kg, q24 h for seven doses (11/12 cats; 92%) were not statistically significant. Results suggest that pradofloxacin can be a safe, efficacious therapy for some cats with suspected bacterial URI.

Efficacy of amoxycillin and azithromycin for the empirical treatment of shelter cats with suspected bacterial upper respiratory infections.

Thirty-one cats showing clinical signs of upper respiratory tract disease with a presumed bacterial component based on clinical signs were administered either amoxycillin or azithromycin to determine which drug protocol was optimal for empirical use. A clinical score was determined and nasal and pharyngeal swabs were collected for bacterial culture, virus isolation and polymerase chain reaction prior to the start of therapy. Cats failing to respond to the initial antibiotic were then administered the other drug. There were no differences in clinical scores between the two groups at the start of therapy. Eleven of 31 cats improved after administration of the first antibiotic, 16 cats were switched to the alternate antibiotic, and four cats were removed from the study for additional supportive treatments. Eight of 27 cats failed to respond to either antibiotic. The chi2 test for outcomes revealed no differences in response to therapy for either antimicrobial.

A lifesaving model: teaching advanced procedures on shelter animals in a tertiary care facility.

It is estimated that there are over 5 million homeless animals in the United States. While the veterinary profession continues to evolve in advanced specialty disciplines, animal shelters in every community lack resources for basic care. Concurrently, veterinary students, interns, and residents have less opportunity for practical primary and secondary veterinary care experiences in tertiary-care institutions that focus on specialty training. The two main goals of this project were (1) to provide practical medical and animal-welfare experiences to veterinary students, interns, and residents, under faculty supervision, and (2) to care for animals with medical problems beyond a typical shelter's technical capabilities and budget. Over a two-year period, 22 animals from one humane society were treated at Colorado State University Veterinary Medical Center. Initial funding for medical expenses was provided by PetSmart Charities. All 22 animals were successfully treated and subsequently adopted. The results suggest that collaboration between a tertiary-care facility and a humane shelter can be used successfully to teach advanced procedures and to save homeless animals. The project demonstrated that linking a veterinary teaching hospital's resources to a humane shelter's needs did not financially affect either institution. It is hoped that such a program might be used as a model and be perpetuated in other communities.

Evaluation of a Community's Risk for Canine Parvovirus and Distemper Using Antibody Testing and GIS Mapping of Animal Shelter Intakes.

This cross-sectional study aimed to identify where dogs with negative antibody tests to canine parvovirus (CPV) and canine distemper virus (CDV) originated when entering a community shelter, using a commercially available ELISA antibody test and Geographic Information Systems mapping. Of 2745 canines entering during a three-month period, 1056 test results were obtained. Dogs or puppies weighing over 2 lb were eligible if they could be humanely, nonchemically restrained for phlebotomy. Age and minor health issues weren't exclusions. Dogs were excluded if trained personnel were concerned health would be compromised by phlebotomy. Blood samples were collected within 24 hours of entry. Four hundred and twenty-seven (40%) dogs had positive antibody test results for both viruses, 422 (40%) were positive for CPV, 37 (4%) were positive for CDV, and 170 (16%) were negative for both. Mapping revealed geographic patterns for dogs with negative antibody tests. This shelter admitted dogs with negative CPV and/or CDV antibody tests from defined community areas. Targeting vaccination efforts in communities to areas where dogs with negative antibody tests originate could be an effective wellness strategy.

A survey of North American shelter practices relating to feline upper respiratory management.

An internet-based survey was conducted to determine common strategies for control of feline upper respiratory infections (URI) in animal shelters. Two hundred and fifty-eight North American shelters responded, representing a spectrum of 57% private non-profit, 27% municipal and 16% combined private non-profit-municipal shelters. All but nine shelters reported having a regular relationship with a veterinarian, 53% had full-time veterinarians and 62% indicated full-time (non-veterinarian) medical staff. However, in 35% of facilities, non-medical shelter management staff determined what medication an individual cat could receive, with 5% of facilities making that decision without indicating the involvement of a veterinarian or technician. Ninety-one percent of shelters had an isolation area for clinically ill cats. The most commonly used antimicrobial was doxycycline (52%), followed by amoxicillin/clavulanic acid (33%). Shelters are using a wide range of prevention measures and therapeutics, leaving room for studying URI in different settings to improve understanding of optimal protocols.

Evaluation of virus isolation, one-step real-time reverse transcription polymerase chain reaction assay, and two rapid influenza diagnostic tests for detecting canine Influenza A virus H3N8 shedding in dogs.

Sustained transmission of canine Influenza A virus (CIV) H3N8 among U.S. dogs underscores the threat influenza continues to pose to canine health. Because rapid and accurate detection of infection is critical to the diagnosis and control of CIV, the 2 main objectives of the current study were to estimate and compare the sensitivities of CIV testing methods on canine swab samples and to evaluate the performance of Flu Detect™ (Synbiotics Corp., Kansas City, MO) for detecting CIV nasal shedding in high-risk shelter dogs. To address the first objective, nasal and pharyngeal swab samples were collected from 124 shelter and household dogs seen by Colorado State University Veterinary Teaching Hospital clinicians for canine infectious respiratory disease between April 2006 and March 2007 and tested for CIV shedding using virus isolation, the rapid influenza diagnostic test Directigen Flu A+B™ (BD Diagnostic Systems, Sparks, MD), and real-time reverse transcription polymerase chain reaction (RT-PCR). For the second objective, 1,372 dogs with unknown respiratory health status were sampled from 6 U.S. shelters from December 2009 to November 2010. Samples were tested for presence of CIV using real-time RT-PCR and Flu Detect. Using a stochastic latent class modeling approach, the median sensitivities of virus isolation, rapid influenza diagnostic test, and real-time RT-PCR were 72%, 65%, and 95%, respectively. The Flu Detect test performed poorly for detecting CIV nasal shedding compared to real-time RT-PCR. In conclusion, the real-time RT-PCR has the highest sensitivity for detecting virus nasal shedding and can be used as a rapid diagnostic test for CIV.