RESUMO
We report the nucleotide sequence of the human chromosomal gene which encodes the interleukin-1 beta protein (IL-1 beta). The gene spans a region of 7.5 kb and the coding part is divided into seven exons. Comparison with the homologous mouse gene reveals that the structural organization is conserved through evolution. In addition to this, human and murine IL-1 beta genes show extensive sequence homology within the intervening sequences.
Assuntos
Genes , Interleucina-1/genética , Sequência de Aminoácidos , Animais , Bacteriófago lambda/genética , Sequência de Bases , Evolução Biológica , Escherichia coli/genética , Éxons , Humanos , Camundongos , Homologia de Sequência do Ácido Nucleico , Especificidade da EspécieRESUMO
A specific nuclear protein (SNP) appears in the oviduct of the lizard, Podarcis s. sicula Raf., during the recovery phase of the breeding cycle. The protein has a low molecular weight (9.9 kDa), a high electrophoretic mobility and a peculiar amino acid composition. It seems to be regulated by estradiol which, in this species, is involved in oviduct stimulation. Nuclear poly(ADPribose)transferase activity increases in the oviduct as the organ grows, and it peaks upon morphological maturation. Thereafter, as the oviduct becomes secretory, the enzyme returns to basal level. A transient increase of poly(ADPribose)transferase precedes the appearance of SNP, which suggests that the two phenomena are related.
Assuntos
Lagartos/fisiologia , Nucleoproteínas/metabolismo , Oviductos/fisiologia , Poli(ADP-Ribose) Polimerases/metabolismo , Animais , Estradiol/fisiologia , Estro , Feminino , Peso Molecular , Estações do AnoRESUMO
The activity of poly (ADP-ribose) polymerase (ADPRP) and the content of 2',5'-oligodenylates core (2',5'An; n = 2,3 and 4) were measured in homogenates of the uterus and of the liver of immature rats immediately before (time 0) or at different times after injection of estradiol-valerate. ADPRP activity increased gradually, starting 6 hours after estrogen injection, for about 4 days. Instead, the content of 2',5'An decreased by about 50% within 6 hours, and thereafter more slowly for 4 days to about 20% of starting values. Estrogen increased ADPRP activity and decreased 2',5'An concentration also in the kidney and in the cardiac muscle of the same animals, but not in the skeletal muscle, where neither of the two parameters was affected. Injection of vehicle only (sesame oil) had no effect on ADPRP activity nor on 2',5'An content of immature rat tissues.