Multicenter Evaluation of the Clinical Performance and the Neutralizing Antibody Activity Prediction Properties of 10 High-Throughput Serological Assays Used in Clinical Laboratories.

As the coronavirus disease 2019 (COVID-19) pandemic second wave is emerging, it is of the upmost importance to screen the population immunity in order to keep track of infected individuals. Consequently, immunoassays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with high specificity and positive predictive values are needed to obtain an accurate epidemiological picture. As more data accumulate about the immune responses and the kinetics of neutralizing-antibody (nAb) production in SARS-CoV-2-infected individuals, new applications are forecast for serological assays such as nAb activity prediction in convalescent-phase plasma from recovered patients. This multicenter study, involving six hospital centers, determined the baseline clinical performances, reproducibility, and nAb level correlations of 10 commercially available immunoassays. In addition, three lateral-flow chromatography assays were evaluated, as these devices can be used in logistically challenged areas. All assays were evaluated using the same patient panels in duplicate, thus enabling accurate comparison of the tests. Seven immunoassays examined in this study were shown to have excellent specificity (98 to 100%) and good to excellent positive predictive values (82 to 100%) when used in a low (5%)-seroprevalence setting. We observed sensitivities as low as 74% and as high as 95% at ≥15 days after symptom onset. The determination of optimized cutoff values through receiver operating characteristic (ROC) curve analyses had a significant impact on the diagnostic resolution of several enzyme immunoassays by increasing the sensitivity significantly without a large trade-off in specificity. We found that spike-based immunoassays seem to be better correlates of nAb activity. Finally, the results reported here will add to the general knowledge of the interlaboratory reproducibility of clinical performance parameters of immunoassays and provide new evidence about nAb activity prediction.

Asymptotic judgment of cause in a relative validity paradigm.

We report three experiments in which we tested asymptotic and dynamic predictions of the Rescorla-Wagner (R-W) model and the asymptotic predictions of Cheng's probabilistic contrast model (PCM) concerning judgments of causality when there are two possible causal candidates. We used a paradigm in which the presence of a causal candidate that is highly correlated with an effect influences judgments of a second, moderately correlated or uncorrelated cause. In Experiment 1, which involved a moderate outcome density, judgments of a moderately positive cause were attenuated when it was paired with either a perfect positive or perfect negative cause. This attenuation was robust over a large set of trials but was greater when the strong predictor was positive. In Experiment 2, in which there was a low overall density of outcomes, judgments of a moderately correlated positive cause were elevated when this cause was paired with a perfect negative causal candidate. This elevation was also quite robust over a large set of trials. In Experiment 3, estimates of the strength of a causal candidate that was uncorrelated with the outcome were reduced when it was paired with a perfect cause. The predictions of three theoretical models of causal judgments are considered. Both the R-W model and Cheng's PCM accounted for some but not all aspects of the data. Pearce's model of stimulus generalization accounts for a greater proportion of the data.

Strategies for generating multiple instances of common and ad hoc categories.

In a free-emission procedure participants were asked to generate instances of a given category and to report, retrospectively, the strategies that they were aware of using in retrieving instances. In two studies reported here, participants generated instances for common categories (e.g. fruit) and for ad hoc categories (e.g., things people keep in their pockets) for 90 seconds and for each category described how they had proceeded in doing so. Analysis of the protocols identified three broad classes of strategy: (1) experiential, where memories of specific or generic personal experiences involving interactions with the category instances acted as cues; (2) semantic, where a consideration of abstract conceptual characteristics of a category were employed to retrieve category exemplars; (3) unmediated, where instances were effortlessly retrieved without mediating cognitions of which subjects were aware. Experiential strategies outnumbered semantic strategies (on average 4 to 1) not only for ad hoc categories but also for common categories. This pattern was noticeably reversed for ad hoc categories that subjects were unlikely to have experienced personally (e.g. things sold on the black market in Russia). Whereas more traditional accounts of semantic memory have favoured decontextualised abstract representations of category knowledge, to the extent that mode of access informs us of knowledge structures, our data suggest that category knowledge is significantly grounded in terms of everyday contexts where category instances are encountered.

[Bronchiolitis caused by respiratory syncytial virus in very young infants and transmitted maternal antibodies]. / Bronchiolites à virus respiratoire syncytial chez le tout jeune enfant et anticorps maternels transmis.

The study with an Enzyme Linked Immunosorbent Assay of the sera of 57 infants aged less than 9 weeks and developing a bronchiolitis, argues for the absence of a protective role for the maternally transmitted anti-respiratory syncytial virus (RSV) IgG and for the existence of a positive relationship between these antibodies levels - which are not neutralizing - and the severity of RSV bronchiolitis.