RESUMO
Striated muscle is now known to contain a third major class of filaments, additional to the thick and thin filaments. The presence of such extra filaments has seemed likely for many years, but details of their location, structure, and composition are only now becoming clear. They are composed of massively large proteins and, in contrast to thick and thin filaments, they are elastic.
Assuntos
Tecido Elástico/fisiologia , Proteínas Musculares/fisiologia , Proteínas Quinases , Sequência de Aminoácidos , Animais , Conectina , Tecido Elástico/ultraestrutura , Dados de Sequência Molecular , Proteínas Musculares/ultraestruturaRESUMO
Recent studies of the giant protein titin have shed light on its roles in muscle assembly and elasticity and include the surprising findings described here. We now know that the titin kinase domain, which has long been a puzzle, has a novel regulation mechanism. A substrate, telethonin, has been identified that is located over one micron away from the kinase domain in mature muscle. Single-molecule studies have demonstrated the fascinating process of reversible mechanical unfolding of titin. Lastly, and most surprisingly, it has been claimed that titin controls assembly and elasticity in chromosomes.
Assuntos
Proteínas Musculares/química , Proteínas Musculares/metabolismo , Músculos/fisiologia , Proteínas Quinases/química , Proteínas Quinases/metabolismo , Animais , Cromossomos/fisiologia , Conectina , Modelos Moleculares , Contração Muscular , Proteínas Musculares/ultraestrutura , Proteínas Quinases/ultraestrutura , Estrutura Secundária de Proteína , Sarcômeros/fisiologia , Sarcômeros/ultraestruturaRESUMO
The interaction of titin with myosin has been studied by binding assays and electron microscopy. Electron micrographs of the titin-myosin complex suggest a binding site near the tip of the tail of the myosin molecule. The distance from the myosin head-tail junction to titin indicates binding 20-30 nm from the myosin COOH terminus. Consistent with this, micrographs of titin-light meromyosin (LMM) show binding near the end of the LMM molecule. Plots of myosin- and LMM-attachment positions along the titin molecule show binding predominantly in the region located in the A band in situ, which is consistent with the proposal that titin regulates thick filament assembly. Estimates of the apparent dissociation constant of the titin-LMM complex were approximately 20 nM. Assays of LMM cyanogen bromide fragments also suggested a strong binding site near the COOH terminus. Proteolysis of a COOH-terminal 17.6-kD CNBr fragment isolated from whole myosin resulted in eight peptides of which only one, comprising 17 residues, bound strongly to titin. Two isoforms of this peptide were detected by protein sequencing. Similar binding data were obtained using synthetic versions of both isoforms. The peptide is located immediately COOH-terminal of the fourth "skip" residue in the myosin tail, which is consistent with the electron microscopy. Skip-4 may have a role in determining thick filament structure, by allowing abrupt bending of the myosin tail close to the titin-binding site.
Assuntos
Proteínas de Membrana/metabolismo , Proteínas Musculares/metabolismo , Miosinas/metabolismo , Proteínas Quinases/metabolismo , Sequência de Aminoácidos , Animais , Cromatografia Líquida de Alta Pressão , Conectina , Brometo de Cianogênio , Interações Medicamentosas , Cadeias J de Imunoglobulina/metabolismo , Proteínas de Membrana/ultraestrutura , Microscopia Eletrônica , Dados de Sequência Molecular , Proteínas Musculares/ultraestrutura , Subfragmentos de Miosina/metabolismo , Subfragmentos de Miosina/ultraestrutura , Miosinas/ultraestrutura , Ligação Proteica/fisiologia , Proteínas Quinases/ultraestrutura , CoelhosRESUMO
Electron microscopy of negatively stained myosin has previously revealed three discrete regions within the heads of the molecule. However, despite a probable resolution of approximately 2 nm, it is difficult to discern directly consistent details within these regions. This is due to variability in both head conformation and in staining. In this study, we applied single-particle image processing and classified heads into homogeneous groups. The improved signal-to-noise ratio after averaging these groups reveals substantially improved detail. The image averages were compared to a model simulating negative staining of the atomic structure of subfragment-1 (S1). This shows that the three head regions correspond to the motor domain and the essential and regulatory light chains. The image averages were very similar to particular views of the S1 model. They also revealed considerable flexibility between the motor and regulatory domains, despite the molecules having been prepared in the absence of nucleotide. This flexibility probably results from rotation of the regulatory domain about the motor domain, where the relative movement of the regulatory light chain is up to 12 nm, and is most clearly illustrated in animated sequences (available at http://www.leeds.ac.uk/chb/muscle/myosinhead.htm l). The sharply curved conformation of the atomic model of S1 is seen only rarely in our data, with straighter heads being more typical.
Assuntos
Miosinas/fisiologia , Miosinas/ultraestrutura , Animais , Galinhas , Processamento de Imagem Assistida por Computador , Microscopia Eletrônica/métodos , Modelos Moleculares , Subfragmentos de Miosina/classificação , Subfragmentos de Miosina/fisiologia , Subfragmentos de Miosina/ultraestrutura , Miosinas/classificação , Tamanho da Partícula , Estrutura Terciária de Proteína , Coelhos , Coloração e RotulagemRESUMO
Titin and nebulin are giant muscle proteins, both of which are approximately 1 micron long and are composed of many repeating domains. Titin domains resemble type III fibronectin and C-2 immunoglobulins. Both proteins are likely to be involved in specifying and stabilizing the highly ordered structure of muscle, probably by acting as 'protein rulers' to regulate the assembly of myosin and actin filaments precisely.
Assuntos
Proteínas Musculares/fisiologia , Músculos/química , Proteínas Quinases , Actinas/metabolismo , Conectina , Elasticidade , Modelos Moleculares , Proteínas Musculares/química , Músculos/ultraestrutura , Fosforilação , Conformação ProteicaRESUMO
Solutions of intact cardiac thin filaments were examined with transmission electron microscopy, dynamic light scattering (DLS), and particle-tracking microrheology. The filaments self-assembled in solution with a bell-shaped distribution of contour lengths that contained a population of filaments of much greater length than the in vivo sarcomere size ( approximately 1 mum) due to a one-dimensional annealing process. Dynamic semiflexible modes were found in DLS measurements at fast timescales (12.5 ns-0.0001 s). The bending modulus of the fibers is found to be in the range 4.5-16 x 10(-27) Jm and is weakly dependent on calcium concentration (with Ca2+ > or = without Ca2+). Good quantitative agreement was found for the values of the fiber diameter calculated from transmission electron microscopy and from the initial decay of DLS correlation functions: 9.9 nm and 9.7 nm with and without Ca2+, respectively. In contrast, at slower timescales and high polymer concentrations, microrheology indicates that the cardiac filaments act as short rods in solution according to the predictions of the Doi-Edwards chopsticks model (viscosity, eta approximately c(3), where c is the polymer concentration). This differs from the semiflexible behavior of long synthetic actin filaments at comparable polymer concentrations and timescales (elastic shear modulus, G' approximately c(1.4), tightly entangled) and is due to the relative ratio of the contour lengths ( approximately 30). The scaling dependence of the elastic shear modulus on the frequency (omega) for cardiac thin filaments is G' approximately omega(3/4 +/- 0.03), which is thought to arise from flexural modes of the filaments.
Assuntos
Citoesqueleto de Actina/química , Biofísica/métodos , Animais , Cálcio/química , Elasticidade , Processamento de Imagem Assistida por Computador , Luz , Microscopia Eletrônica de Transmissão , Músculo Esquelético/metabolismo , Miocárdio/metabolismo , Reologia , Espalhamento de Radiação , Suínos , ViscosidadeRESUMO
The complete sequence of the giant muscle protein titin has been determined. It provides further insight into how titin may act both as a scaffold and a spring to specify and maintain muscle structure.
Assuntos
Citoesqueleto/fisiologia , Proteínas Musculares/fisiologia , Proteínas Quinases/fisiologia , Animais , Conectina , Elasticidade , Humanos , Músculo Esquelético/fisiologiaRESUMO
The effect of temperature on the length of the tail of the myosin molecule has been studied by negative staining of molecules immobilized on carbon substrates at different temperatures. In buffers containing chloride as the principal anion, tail length was approximately constant up to 25 degrees C. Above this temperature, it shortened linearly with increasing temperature up to 42 degrees C, the highest temperature studied in this solvent. The amount of shortening per degree C was about 1.2 nm. A similar amount of shortening per degree C was seen in acetate-containing buffers up to 50 degrees C, but in this case it did not begin until the temperature exceeded about 40 degrees C. A large fraction of the observed shortening was localized in a region that lies roughly between the two positions in the tail where proteolysis results in production of short or long subfragment-2. Frequently, the tail had a different appearance in this region from elsewhere and could sometimes be seen to split into two strands that were separate but coiled around one another.
Assuntos
Miosinas , Temperatura , Substâncias Macromoleculares , Microscopia Eletrônica , Subfragmentos de MiosinaRESUMO
Muscle elasticity derives directly from titin extensibility, which stems from three distinct types of spring-like behaviour of the I-band portion of the molecule. With progressively greater forces and sarcomere lengths, the molecule straightens and then unfolds, first in the PEVK-region and then in individual immunoglobulin domains. Here, we report quantitative analysis of flexibility and extensibility in isolated titin molecules visualized by electron microscopy. Conformations displayed by molecules dried on a substrate vary from a random coil to rod-like, demonstrating highly flexible and easily deformable tertiary structure. The particular conformation observed depends on the "wettability" of the substrate during specimen preparation: higher wettability favours coiled conformations, whereas lower wettability results in more extended molecules. Extension is shown to occur during liquid dewetting. Statistical methods of conformational analysis applied to a population of coiled molecules gave an average persistence length 13.5(+/-4.5) nm. The close correspondence of this value to an earlier one from light-scattering studies confirms that conformations observed by microscopy closely reflected the equilibrium conformation in solution. Analysis of hydrodynamic forces exerted during dewetting also indicates that the force causing straightening of the molecules and extension of the PEVK-region is in the picoNewton range, whereas unfolding of the immunoglobulin and fibronectin domains may require forces about tenfold higher. The microscope data directly illustrate the relationship between titin conformation and the magnitude of applied force. They also suggest the presence of torsional stiffness in the molecule, which may affect considerations of elasticity.
Assuntos
Proteínas Musculares/química , Proteínas Musculares/ultraestrutura , Proteínas Quinases/química , Proteínas Quinases/ultraestrutura , Animais , Conectina , Elasticidade , Fibronectinas/química , Imunoglobulinas/química , Microscopia Eletrônica/métodos , Proteínas Musculares/metabolismo , Músculo Esquelético , Maleabilidade , Proteínas Quinases/metabolismo , Estrutura Terciária de Proteína , Coelhos , Termodinâmica , Água/metabolismoRESUMO
The heads of myosin molecules from the striated adductor muscle of scallop have been studied by electron microscopy after negative staining. In common with vertebrate skeletal muscle myosin visualized by this method, the scallop myosin heads were pear-shaped and often showed pronounced curvature. Staining suggestive of two or, more frequently, three domains could often be observed. Removal of regulatory light chains (R-LCs) resulted in a reduction in the length of the heads of about 2.6 nm, with no significant change in maximum width. In desensitized preparations a majority of heads displayed anticlockwise curvature, whereas intact heads were usually seen curved clockwise. Analysis of the head curvature in both intact and desensitized molecules was consistent with an ability of each head to rotate about its long axis. Desensitization resulted in an increased incidence of heads showing two domains. It seems likely that the reduction in length upon removal of the R-LC is due to the two small domains located in the neck region of the head collapsing into one.
Assuntos
Moluscos/ultraestrutura , Músculos/ultraestrutura , Subfragmentos de Miosina , Miosinas , Animais , Microscopia EletrônicaRESUMO
"Molecular combing" induced by a receding meniscus has been shown to extend individual titin molecules. Electron microscopy reveals that both ends of the molecule tend to attach to a mica substrate, probably due to their local positive charges. This leaves the remainder of the molecule free to be straightened and extended by forces of up to approximately 800 pN. A small region in the I-band part of the molecule, which probably corresponds to sequence high in P, E, V and K residues, is the most compliant and appears to extend by an unfolding of the polypeptide chain. Other parts of the molecule are also capable of extension. These mechanical extensions in titin are probably reversible.
Assuntos
Proteínas Musculares/química , Conformação Proteica , Proteínas Quinases/química , Silicatos de Alumínio , Sequência de Aminoácidos , Fenômenos Químicos , Físico-Química , Conectina , Elasticidade , Microscopia Eletrônica , Proteínas Musculares/ultraestrutura , Dobramento de Proteína , Proteínas Quinases/ultraestrutura , Tensão SuperficialRESUMO
It is shown that an interaction exists between AMP deaminase (EC 3.5.4.6) and myofibrils that is sufficiently strong (Kd congruent to 10(-10) M) for more than 99% of the binding sites for the enzyme to be filled in vivo. The binding is not strong enough, however, to stop removal of the enzyme during the extensive washing normally used in the preparation of myofibrils. Fluorescent antibodies to the enzyme label myofibrils close to the junction of the A- and I-bands. The invariance of the position of the antibody stripes at this site, over a range of sarcomere lengths, indicates that the enzyme is attached to the A-band. The intensity of the fluorescence declines in parallel with dissociation of the enzyme. In this muscle, the number of AMP deaminase binding sites per thick filament is approximately six, suggesting that the enzyme is located at a single axial position in each half A-band. Electron microscopy of negatively stained, antibody-labelled myofibrils reveals the distance between the AMP deaminase sites at opposite ends of an A-band to be 1.69(+/- 0.02 micron). Since the length of the A-band is 1.57 micron, the binding site for the enzyme must be significantly beyond where thick filaments have previously been thought to end.
Assuntos
AMP Desaminase/metabolismo , Miofibrilas/enzimologia , Nucleotídeo Desaminases/metabolismo , Animais , Sítios de Ligação , Imunofluorescência , Microscopia Eletrônica , Miofibrilas/ultraestrutura , CoelhosRESUMO
Rabbit psoas muscle filaments, isolated in relaxing buffer from non-glycerinated muscle, have been applied to hydrophilic carbon films and stained with uranyl acetate. Electron micrographs were obtained under low-dose conditions to minimize specimen damage. Surrounding the filament backbone, except in the bare zone, is a fringe of clearly identifiable myosin heads. Frequently, both heads of individual myosin molecules are seen, and sometimes a section of the tail can be seen connecting the heads to the backbone. About half the expected number of heads can be counted, and they are uniformly distributed along the filament. The majority of heads appear curved. The remainder could be curved heads viewed from another aspect. Three times as many heads curve in a clockwise sense than in an anticlockwise sense, suggesting a preferential binding of one side of the head to the carbon film. The two heads of myosin molecules exhibit all the possible combinations of clockwise, anticlockwise and straight heads, and analysis of their relative frequencies suggests that the heads rotate freely and independently. The heads also adopt a wide range of angles of attachment to the tail. The lengths of heads cover a range of 14 to 26 nm, with a peak at 19 nm. The average maximum width is 6.5 nm. Both measurements are in excellent agreement with values for shadowed molecules. Since our data are from heads adsorbed to the film in relaxing conditions and the shadowed molecules were free of nucleotide, gross shape changes are not likely to be produced by nucleotide binding. The length of the link between the heads and the backbone was found to vary between 10 nm and 52 nm, with a broad peak at about 25 nm. Thus, the hinge point detected in the tail of isolated molecules was not usually the point from which the crossbridges swung out from the filament surface. The angle made by the link to the filament axis was between 20 degrees and 80 degrees, with a broad maximum around 45 degrees. These lengths and angles concur with our observation of an average limit of the crossbridges from the filament surface of 30 nm. This is sufficient to enable heads in the myofibril lattice to reach out beyond the nearest thin filament and should allow considerable flexibility for stereospecific binding to actin in active muscle.
Assuntos
Citoesqueleto/ultraestrutura , Músculos/ultraestrutura , Miosinas , Animais , Citoesqueleto/análise , Substâncias Macromoleculares , Microscopia Eletrônica , Músculos/análise , CoelhosRESUMO
The protein titin has been localized by electron microscopy of myofibrils labelled with monoclonal antibodies. The data are consistent with individual titin molecules extending from near the M-line to beyond the ends of thick filaments, a distance of approximately 1 micron. In the A-band, titin appears to be bound to thick filaments, probably to the outside of the filament shaft. Molecules of titin in this configuration provided an obvious mechanism by which the length of thick filaments could be regulated accurately.
Assuntos
Proteínas Musculares/metabolismo , Músculos/metabolismo , Miofibrilas/ultraestrutura , Proteínas Quinases , Animais , Conectina , Camundongos , Microscopia Eletrônica , Músculos/ultraestruturaRESUMO
A reproducible method has been developed for the negative staining of myosin molecules. The dimensions of stained molecules are in close agreement with those obtained by metal shadowing. Sharp bends in the tail, indicative of hinge regions, were observed at two positions 44 nm and 76 nm from the head-tail junction. The tail was often ill-defined at the position of the first (44 nm) bend. The bend positions may be sites of proteolytic cleavage that result in the production of long and short myosin subfragment S2. About half the molecules exhibited bending to various degrees at one or both of these positions, but cases where the tail folded back on itself in a 180 degrees bend were comparatively rare (approximately equal to 10%). However, in the absence of EGTA, a large fraction of the molecules (approximately equal to 80%) exhibited 180 degrees bends. A small region, approximately 20 nm long, at the tip of the tail often appears to be significantly different from the rest. The heads are about 19 nm long and roughly pear-shaped. Although sometimes straight, more often they show a pronounced curvature. Both senses of curvature were observed, but those curved in a clockwise manner were the most common, indicating preferential binding of one side of the head to the carbon substrate. An analysis of the different combinations of head shapes in individual molecules indicates that each head can rotate independently around its long axis. No preferred angle of orientation between the two heads in a molecule, or between either head and the tail could be found. Substructure has been observed within the heads.
Assuntos
Miosinas , Animais , Ácido Egtázico , Microscopia Eletrônica/métodos , Músculos/análise , Coelhos , Coloração e RotulagemRESUMO
A procedure has been developed for the extraction and purification of the massive myofibrillar protein titin without exposing it to denaturing conditions. The form of the molecule that has been isolated is soluble at high ionic strength and alkaline pH, but precipitates in low salt or at pH values below 7. Sedimentation velocity experiments indicate that titin is a highly asymmetric molecule with a sedimentation coefficient of 13.4 S. This asymmetry is confirmed by electron microscopy of rotary-shadowed specimens, which shows string-like structures of diameter 40 A and lengths up to 8000 A. Significant differences were observed depending on whether the electron microscope specimens were prepared by spraying or by layering of the titin onto a mica substrate; we tentatively attribute these differences to elasticity in the titin, revealed by the high shearing forces that accompany spraying. In accord with this, the circular dichroism spectrum of titin indicates that its secondary structure is largely random coil, a conformation characteristic of elastic proteins such as elastin. Negative staining of titin again shows long string-like structures, but these can now be seen to have an appearance similar to a string of beads, where the spacing between successive beads is about 40 A. Very similar beaded strings have been observed also associated with negatively stained separated native thick filaments; these are found running alongside the cross-bridge regions and in coils near the filament ends. Since the periodicity of the strings is similar to that of end-filaments, recently identified structures at the tips of thick filaments, it is likely that end-filaments are formed from titin. Titin comprises approximately 9% of the myofibrillar mass, which means that it is the third most abundant protein in muscle. The possible role of titin in forming elastic filaments within myofibrils is discussed.
Assuntos
Proteínas Musculares/isolamento & purificação , Proteínas Quinases , Animais , Cromatografia DEAE-Celulose , Dicroísmo Circular , Conectina , Eletroforese em Gel de Poliacrilamida , Microscopia Eletrônica , Miofibrilas/análise , Coelhos , Solubilidade , UltracentrifugaçãoRESUMO
Titin is thought to play a major role in myofibril assembly, elasticity and stability. A single molecule spans half the sarcomere and makes interactions with both a thick filament and the Z-line. In the unit cell structure of each half sarcomere there is one thick filament with 3-fold symmetry and two thin filaments with approximately 2-fold symmetry. The minimum number of titin molecules that could satisfy both these symmetries is 12. We determined the actual number of titin molecules in a unit cell from scanning transmission electron microscopy mass measurements of end-filaments. One of these emerges from each tip of the thick filament and is thought to be the in-register aggregate of the titin molecules associated with the filament. The mass per unit length of the end-filament (17.1 kDa/nm) is consistent with six titin molecules not 12. Thus the number of titin molecules present is insufficient to satisfy both symmetries. We suggest a novel solution to this paradox in which four of the six titin molecules interact with the two thin filaments in the unit cell, while the remaining two interact with the two thin filaments that enter the unit cell from the adjacent sarcomere. This arrangement would augment mechanical stability in the sarcomere.
Assuntos
Citoesqueleto de Actina/química , Citoesqueleto de Actina/ultraestrutura , Proteínas Musculares/análise , Proteínas Musculares/ultraestrutura , Proteínas Quinases/análise , Proteínas Quinases/ultraestrutura , Sarcômeros/química , Sarcômeros/ultraestrutura , Citoesqueleto de Actina/metabolismo , Animais , Galinhas , Conectina , Camundongos , Microscopia Eletrônica de Transmissão e Varredura , Modelos Moleculares , Peso Molecular , Proteínas Musculares/metabolismo , Proteínas Quinases/metabolismo , Coelhos , Rana pipiens , Ratos , Sarcômeros/metabolismo , SuínosRESUMO
Individual molecules of the giant muscle proteins titin and nebulin span large distances in the sarcomere. Approximately one-third of the titin molecule forms elastic filaments linking the ends of thick filaments to the Z-line. The remainder of the molecule is probably bound to the thick filament where it may regulate assembly of myosin and the other thick filament proteins. This region also contains a sequence similar to catalytic domains in protein kinases. Nebulin appears to be associated with thin filaments and may regulate actin assembly.
Assuntos
Proteínas de Transporte/fisiologia , Proteínas de Membrana/fisiologia , Proteínas Musculares/fisiologia , Proteínas Quinases , ConectinaRESUMO
Partial amino acid sequence was obtained from the massive myofibrillar protein nebulin. This consists of repeating motifs of about 35 residues and super-repeats of 7 x 35 = 245 residues. The repeat-motifs are likely to be largely alpha-helical and to interact with both actin and tropomyosin in thin filaments. Nebulin from different species was found to vary in size in proportion to filament length. The data are consistent with the proposal that nebulin acts as a protein-ruler to regulate precise thin filament assembly.
Assuntos
Proteínas Musculares/fisiologia , Citoesqueleto de Actina/ultraestrutura , Sequência de Aminoácidos , Animais , Bovinos , Galinhas , DNA/genética , Humanos , Dados de Sequência Molecular , Proteínas Musculares/química , CoelhosRESUMO
Molecules of the giant protein titin are responsible for the passive elasticity and central A-band location of muscle myofibrils. The molecular mechanism of titin elasticity is not known, but the I-band region of the molecule appears capable of approximately fourfold reversible extension. Such large extensions are likely to involve unfolding of titin domains. In the present experiments, equilibrium unfolding of titin from rabbit skeletal muscles was studied in vitro by fluorescence and circular dichroism spectroscopy, after addition of guanidinium chloride. The data suggest two unfolding transitions, both of which appear cooperative. The second transition is likely to involve complete unfolding of the immunoglobulin- and fibronectin-like domains from which the molecules is composed. The free energy associated with this transition is comparable with the energy required to extend titin molecules to the maximum amount seen in situ.