RESUMO
Growing evidence suggests that sleep could affect the immunological response after vaccination. The aim of this prospective study was to investigate possible associations between regular sleep disruption and immunity response after vaccination against coronavirus disease 2019 (COVID-19). In total, 592 healthcare workers, with no previous history of COVID-19, from eight major Greek hospitals were enrolled in this study. All subjects underwent two Pfizer-BioNTech messenger ribonucleic acid (mRNA) COVID-19 vaccine BNT162b2 inoculations with an interval of 21 days between the doses. Furthermore, a questionnaire was completed 2 days after each vaccination and clinical characteristics, demographics, sleep duration, and habits were recorded. Blood samples were collected and anti-spike immunoglobulin G antibodies were measured at 20 ± 1 days after the first dose and 21 ± 2 days after the second dose. A total of 544 subjects (30% males), with median (interquartile range [IQR]) age of 46 (38-54) years and body mass index of 24·84 (22.6-28.51) kg/m2 were eligible for the study. The median (IQR) habitual duration of sleep was 6 (6-7) h/night. In all, 283 participants (52%) had a short daytime nap. In 214 (39.3%) participants the Pittsburgh Sleep Quality Index score was >5, with a higher percentage in women (74·3%, p < 0.05). Antibody levels were associated with age (r = -0.178, p < 0.001), poor sleep quality (r = -0.094, p < 0.05), insomnia (r = -0.098, p < 0.05), and nap frequency per week (r = -0.098, p < 0.05), but after adjusting for confounders, only insomnia, gender, and age were independent determinants of antibody levels. It is important to emphasise that insomnia is associated with lower antibody levels against COVID-19 after vaccination.
Assuntos
COVID-19 , Coronavírus Relacionado à Síndrome Respiratória Aguda Grave , Distúrbios do Início e da Manutenção do Sono , Masculino , Feminino , Humanos , Pessoa de Meia-Idade , Duração do Sono , COVID-19/prevenção & controle , Vacinas contra COVID-19 , Vacina BNT162 , Estudos Prospectivos , VacinaçãoRESUMO
The present study aimed to analyse and compare the distribution of MICA (rs1051792) and NKG2D/KLRK1 (rs1154831, rs1049174, rs2255336) polymorphisms in 61 Greek and 100 Polish patients with rheumatoid arthritis in relation to the presence of the HLA-DRB1 shared epitope and clinical parameters. Genotyping of selected polymorphism was performed using real-time PCR. HLA-DRB1 shared epitope alleles segregated differently in Greek and Polish patients but in both populations were detected in over 60% of cases. The rs1051792-A variant was more common among SE-positive Polish patients (p = 0.003) while the rs1049174-G allele was more frequently observed in Greeks than in Poles (p < 0.001). Moreover, among Greek patients, the rs1051792-GG homozygotes more frequently presented with anti-CCP antibodies and rheumatoid factor (RF), while carriers of the rs1049174-G variant and rs1154831-CC homozygotes were characterized by lower disease activity scores (p < 0.05 in all cases). These results imply that, in addition to the HLA-DRB1 SE alleles, MICA and NKG2D polymorphisms may also play a role in rheumatoid arthritis.
RESUMO
BACKGROUND: Listeria monocytogenes is an opportunistic pathogen of the central nervous system commonly associated with impaired cell-mediated immunity. We hereby present a case of adult neurolisteriosis where the only immunological feature persistently present was serum IgM deficiency, suggesting that non-specific humoral immunity may also play a central role in the control of neuroinvasion by Listeria monocytogenes. CASE PRESENTATION: A 62-year-old male who had never experienced severe infections presented with headache, nuchal rigidity and confusion. Neuroimaging was normal and lumbar puncture revealed pleiocytosis (760 leukocytes/mm3) and hypoglycorrhachia (34 mg/dL). The patient was treated empirically for bacterial meningitis. Indeed, further analysis of the CSF showed infection by Listeria monocytogenes, which was accompanied by reduced serum IgM levels that persisted well beyond the period of acute bacterial infection. Levels of IgG and IgA isotypes, along with peripheral blood counts of major leukocyte subsets, were at the same time largely preserved. Intriguingly, the absence of membrane-bound IgM on B cells was essentially complete in the acute post-infection period leading to a remarkable recovery after 12 months, suggesting that mechanisms other than defective membrane expression are underlying serum deficiency. CONCLUSIONS: As far as we know, this is the first reported case of neurolisteriosis associated with IgM deficiency in an adult individual without a history of severe infections or other underlying conditions. A possible role of circulating IgM against invasive disease caused by Listeria monocytogenes, particularly in the early course of host-pathogen interaction, is discussed.
Assuntos
Hospedeiro Imunocomprometido , Imunoglobulina M/deficiência , Meningite por Listeria/imunologia , Humanos , Síndromes de Imunodeficiência/complicações , Masculino , Pessoa de Meia-IdadeRESUMO
Anti-Ro52 autoantibody (autoAb), highly prevalent in Sjogren's syndrome (SjS) and systemic lupus erythematosus (SLE), is also frequent in systemic sclerosis (SSc). Viral agents, such as human cytomegalovirus (HCMV), have been considered as a trigger for SSc and SSc-associated autoAbs. To seek for antigen-specific anti-HCMV associations with anti-Ro52, we assessed the dominant anti-HCMV ab responses in anti-Ro52 antibody (ab)-positive and -negative patients with SSc and compared them with those in SLE and SjS. 116 Anti-HCMV ab(+) sera were analyzed, including 70 from anti-Ro52(+) patients (29 SSc, 23 SLE and 18 SjS) and 46 from anti-Ro52(-) patients (29 with SSc, 9 with SLE and 8 with SjS) as negative controls. Abs against specific HCMV pp130/UL57, pp65/UL83, pp55/UL55, pp52/UL44, p38 and pp28/UL99 antigens were tested by immunoblotting. Anti-Ro52(+) SSc patients reacted more frequently against pp52/UL44 and p38 compared to anti-Ro52(-) [(13/29, 44.8%; 95% CI 26.7-62.9% vs. 1/29, 3.4%; 95% CI 0-10%, p < 0.001, and 9/29, 31.0%; 95% CI 14.2-47.8% vs. 2/29, 6.9%; 95% CI 0-16.1%, p = 0.041, respectively]. No such differences were noted between anti-Ro52(+) and anti-Ro52(-) SLE or SjS patients. Also, antibody titres against HCMV pp65/UL83, pp52/UL44 and p38 antigens were higher in anti-Ro52(+) than anti-Ro52(-) SSc patients (p < 0.01). Ab responses against specific HCMV antigens differ among anti-Ro52 ab-positive and -negative patients with SSc (as well as between SSc and SLE or SjS), but whether these differences are epiphenomenal remains to be seen.
Assuntos
Autoanticorpos/sangue , Escleroderma Sistêmico/imunologia , Biomarcadores/sangue , Estudos de Casos e Controles , Feminino , Humanos , Lúpus Eritematoso Sistêmico/sangue , Lúpus Eritematoso Sistêmico/imunologia , Masculino , Ribonucleoproteínas/sangue , Ribonucleoproteínas/imunologia , Escleroderma Sistêmico/sangue , Síndrome de Sjogren/sangue , Síndrome de Sjogren/imunologiaRESUMO
AIM: This study assessed the presence of specific antibodies for coeliac disease in outpatients suffering from eating disorders before and after nutritional intervention. We also evaluated whether those patients should undergo regular screening for coeliac disease. METHODS: The sample consisted of 154 patients with a mean age of 16.7 years - ranging from one to 19 years of age - suffering from eating disorders. Serology screening for coeliac disease and total immunoglobulin A (IgA) levels was evaluated in the 154 children before the nutritional intervention and in 104 patients after the intervention. The patients consumed an adequate amount of gluten in both phases. RESULTS: Postintervention evaluation revealed that 92 patients (88.5%) achieved a normal body weight, while the remaining 12 (11.5%) became obese. Postprandial abdominal discomfort and pain were resolved. The serology tests were negative in all patients, before and after intervention. None displayed IgA deficiency. CONCLUSION: To the best of our knowledge, this was the first prospective study where patients underwent a screening serology for coeliac disease before and after nutritional intervention. No indication of the coexistence of eating disorders and coeliac disease was documented, and the patients in our study were unlikely to require regular screening for coeliac disease.
Assuntos
Doença Celíaca/complicações , Doença Celíaca/diagnóstico , Transtornos da Alimentação e da Ingestão de Alimentos/complicações , Transtornos da Alimentação e da Ingestão de Alimentos/terapia , Adolescente , Índice de Massa Corporal , Doença Celíaca/sangue , Criança , Pré-Escolar , Transtornos da Alimentação e da Ingestão de Alimentos/sangue , Feminino , Humanos , Imunoglobulina A/sangue , Lactente , Masculino , Estudos Prospectivos , Aumento de Peso , Adulto JovemRESUMO
Characterisation of the novel HLA-C*14:02:01:31 allele in a 21-year-old Greek bone marrow donor.
Assuntos
Alelos , Éxons , Antígenos HLA-C , Sequenciamento de Nucleotídeos em Larga Escala , Teste de Histocompatibilidade , Doadores de Tecidos , Humanos , Antígenos HLA-C/genética , Adulto Jovem , Transplante de Medula Óssea , Medula Óssea , Sequência de Bases , Polimorfismo de Nucleotídeo Único , CódonRESUMO
HLA-C*01:02:01:70 differs from the HLA-C*01:02:01:01 allele by one nucleotide substitution in the intron 4.
Assuntos
Genes MHC Classe I , Antígenos HLA-C , Humanos , Antígenos HLA-C/genética , Grécia , Alelos , ÍntronsRESUMO
HLA-B*51:01:01:109 differs from HLA-B*51:01:01:01 by one nucleotide substitution in intron 5.
Assuntos
Antígenos HLA-B , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Alelos , Grécia , Antígenos HLA-B/genéticaRESUMO
HLA-A*02:01:01:243 differs from the HLA-A*02:01:01:01 allele by one nucleotide substitution in the 5'UTR.
Assuntos
Antígenos HLA-A , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Alelos , Grécia , Regiões 5' não Traduzidas , Antígenos HLA-A/genéticaRESUMO
The HLA-DQB1*03:01:01:64 allele differs from HLA-DQB1*03:01:01:03 by a single nucleotide substitution in intron 2.
Assuntos
Alelos , Cadeias beta de HLA-DQ , Sequenciamento de Nucleotídeos em Larga Escala , Íntrons , Humanos , Cadeias beta de HLA-DQ/genética , Teste de Histocompatibilidade/métodos , Éxons , Polimorfismo de Nucleotídeo Único , Análise de Sequência de DNA/métodos , Sequência de BasesRESUMO
The HLA-DQB1*06:02:01:40 allele differs from HLA-DQB1*06:02:01:01 by a single nucleotide substitution in intron 2.
Assuntos
Alelos , Cadeias beta de HLA-DQ , Sequenciamento de Nucleotídeos em Larga Escala , Teste de Histocompatibilidade , Íntrons , Humanos , Cadeias beta de HLA-DQ/genética , Teste de Histocompatibilidade/métodos , Éxons , Polimorfismo de Nucleotídeo Único , Sequência de Bases , Análise de Sequência de DNA/métodosRESUMO
Characterisation of the novel HLA-C*07:01:01:141 allele in a 23-year-old Greek bone marrow donor.
Assuntos
Alelos , Éxons , Antígenos HLA-C , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Antígenos HLA-C/genética , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Adulto Jovem , Teste de Histocompatibilidade/métodos , Doadores de Tecidos , Sequência de Bases , Polimorfismo de Nucleotídeo Único , Alinhamento de Sequência , Análise de Sequência de DNA/métodosRESUMO
HLA-B*07:02:01:110 differs from the HLA-B*07:02:01:01 allele by two nucleotide substitutions in the 3'UTR.
Assuntos
Regiões 3' não Traduzidas , Alelos , Sequência de Bases , Sequenciamento de Nucleotídeos em Larga Escala , Teste de Histocompatibilidade , Humanos , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Teste de Histocompatibilidade/métodos , Antígeno HLA-B7/genética , Éxons , Análise de Sequência de DNA/métodos , Alinhamento de SequênciaRESUMO
HLA-A*32:01:01:41 differs from the HLA-A*32:01:01:01 allele by one nucleotide substitution in the intron 3.
Assuntos
Alelos , Éxons , Antígenos HLA-A , Sequenciamento de Nucleotídeos em Larga Escala , Teste de Histocompatibilidade , Íntrons , Humanos , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Antígenos HLA-A/genética , Teste de Histocompatibilidade/métodos , Sequência de Bases , Polimorfismo de Nucleotídeo Único , Análise de Sequência de DNA/métodos , Alinhamento de SequênciaRESUMO
HLA-B*38:01:01:18 differs from the HLA-B*38:01:01:01 allele by one nucleotide substitution in the 5'UTR.
Assuntos
Regiões 5' não Traduzidas , Alelos , Antígenos HLA-B , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Antígenos HLA-B/genética , Éxons , Teste de Histocompatibilidade/métodos , Sequência de Bases , Polimorfismo de Nucleotídeo Único , Alinhamento de Sequência , Análise de Sequência de DNA/métodosRESUMO
HLA-C*04:01:01:182 differs from the HLA-C*04:01:01:06 allele by one nucleotide substitution in the 5'UTR.
Assuntos
Alelos , Antígenos HLA-C , Sequenciamento de Nucleotídeos em Larga Escala , Teste de Histocompatibilidade , Doadores de Tecidos , Humanos , Antígenos HLA-C/genética , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Teste de Histocompatibilidade/métodos , Regiões 5' não Traduzidas , Éxons , Sequência de Bases , Análise de Sequência de DNA/métodos , Medula Óssea , Polimorfismo de Nucleotídeo Único , Transplante de Medula ÓsseaRESUMO
HLA-C*17:01:01:29 differs from the HLA-C*17:01:01:05 allele by one nucleotide substitution in the 3'UTR.
Assuntos
Genes MHC Classe I , Antígenos HLA-C , Humanos , Antígenos HLA-C/genética , Grécia , Alelos , Regiões 3' não TraduzidasRESUMO
HLA-C*04:01:01:174 differs from the HLA-C*04:01:01:06 allele by one nucleotide substitution in the intron 5.
Assuntos
Genes MHC Classe I , Antígenos HLA-C , Humanos , Antígenos HLA-C/genética , Grécia , Alelos , ÍntronsRESUMO
HLA-B*47:01:01:07 differs from the HLA-B*47:01:01:03 allele by one nucleotide deletion in the 3'UTR.
Assuntos
Medula Óssea , Genes MHC Classe I , Humanos , Alelos , Grécia , Antígenos HLA-B/genéticaRESUMO
HLA-B*41:02:01:11 and -C*08:266 were detected in a solid organ recipient during the HLA typing process.