Assuntos
Doenças do Cão/parasitologia , Leishmania infantum/enzimologia , Leishmaniose Visceral/veterinária , Animais , Doenças do Cão/enzimologia , Doenças do Cão/epidemiologia , Cães , Isoenzimas/classificação , Leishmaniose Visceral/enzimologia , Leishmaniose Visceral/epidemiologia , Espanha/epidemiologiaRESUMO
The partial mitochondrial cytochrome c-oxidase subunit 1 gene (cox 1) and partial mitochondrial 16S ribosomal DNA of Trichuris skrjabini (Baskakov 1924) isolated from Capra hircus have been amplified and sequenced. The analyses of multiple sequence alignments of mitochondrial 16S rDNA and cox 1 of T. skrjabini revealed high homology with those of Trichinella species. For the first time, the mitochondrial DNA gene sequences of one species of trichurid nematode have been cited.
Assuntos
DNA de Helmintos/genética , DNA Mitocondrial/genética , DNA Ribossômico/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , Trichuris/genética , Animais , DNA de Helmintos/química , DNA Mitocondrial/química , DNA Ribossômico/química , Cabras/parasitologia , Dados de Sequência Molecular , RNA Ribossômico 16S/genética , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência , Trichinella/genética , Trichuris/enzimologia , Trichuris/isolamento & purificaçãoRESUMO
In this paper, a morphological and biometrical study by optical microscopy and scanning electronic microscopy (SEM) of Trichuris suis isolated from different hosts (Sus scrofa domestica and Sus scrofa scrofa) and Trichuris trichiura isolated from chimpanzee, has been carried out. Our results demonstrate the existence of typical pericloacal papillae in both species. Biometrical parameters of T. suis and T. trichiura overlapped but males and females of T. trichiura tended to be shorter and thinner than those of T. suis. Our results suggest that T. suis and T. trichiura cannot be differentiated using standard procedures as morphological and biometrical determinations. Thus, the ITS1-5.8S-ITS2 region of the ribosomal DNA was sequenced to allow a differentiation between T. suis and T. trichiura on genetic level. The ITS1 and ITS2 sequences derived from T. trichiura eggs isolated from feces of primates (Colobus guereza kikuyensis and Nomascus gabriellae) showed clear differences to the respective sequences of T. suis derived from eggs of different porcine hosts. The 5.8S gene was similar between the two species. Sequences obtained from different populations of the same species showed no significant differences indicating that the ITS1-5.8S-ITS2 sequences reported in this study are representative for T. trichiura and T. suis, respectively. Phylogenetic relationships have been determined attending to the ITS1 and ITS2 sequences from different species of the genus Trichuris. In conclusion, T. trichiura and T. suis are considered to be closely related but genetically different species. Both species can be easily and reliably distinguished by a PCR-RFLP analysis of the ITS1 and ITS2 sequences with different restriction enzymes.
Assuntos
Tricuríase/veterinária , Trichuris/anatomia & histologia , Trichuris/classificação , Animais , Sequência de Bases , Análise por Conglomerados , DNA de Helmintos/química , DNA de Helmintos/genética , DNA Intergênico/química , DNA Intergênico/genética , Feminino , Masculino , Microscopia/métodos , Microscopia Eletrônica de Varredura/métodos , Dados de Sequência Molecular , Pan troglodytes/parasitologia , Filogenia , RNA Ribossômico 5,8S/química , RNA Ribossômico 5,8S/genética , Análise de Sequência de DNA , Homologia de Sequência , Sus scrofa/parasitologia , Tricuríase/parasitologia , Trichuris/genética , Trichuris/isolamento & purificaçãoRESUMO
Four species of the genus Vannella have been isolated and identified from samples of different freshwater habitats. The present work is an attempt to bring up to date the descriptions of V. simplex, V. platypodia, V. mira and V. miroides.
Assuntos
Amoeba/isolamento & purificação , Água Doce , Água , Amoeba/classificação , Amoeba/ultraestrutura , Animais , Espanha , Especificidade da Espécie , Piscinas , Abastecimento de ÁguaRESUMO
The complete internal transcribed spacer 1 (ITS1), 5.8S rDNA and ITS2 region of the ribosomal DNA from 11 species of rhinonyssid mites ( Tinaminyssus columbae, T. minisetosum, T. sartbaevi, T. bubulci, T. melloi, T. streptopelioides, Sternostoma fulicae, S. boydi, S. strandtmanni, S. turdi, Rhinonyssus tringae) were sequenced to assess the utility of this genomic region in resolving taxonomic questions in this group and to estimate phylogenetic relationships between species. Two different geographic locations of T. melloi and T. streptopelioides were analyzed to detect intraspecies variation. Our study shows that ribosomal sequences can help to discriminate between T. melloi and T. sartbaevi, which are morphologically very close and difficult to separate by classic methods. The resulting phylogenetic tree shows some differences from the current taxonomy of the family Rhinonyssidae. This study appeals for the revision of the taxonomic status of S. boydi and closely related species which parasitize aquatic birds and suggests the synonymy of S. boydi and S. strandtmanni, despite the different hosts of the two mites.
Assuntos
Acaridae/genética , DNA Ribossômico/genética , Acaridae/classificação , Animais , Sequência de Bases , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase , Alinhamento de Sequência , Homologia de Sequência do Ácido Nucleico , Especificidade da EspécieRESUMO
A 390 bp region of the 16S rDNA gene was sequenced from six species of rhinonyssid mites (Tinaminyssus columbae, T. minisetosum, Sternostoma turdi, S. sternahirundo, S. fulicae and Ptilonyssus euroturdi) and two subspecies (Tinaminyssus melloi melloi and Tinaminyssus melloi streptopeliae) to examine the level of sequence variation and the taxonomic levels to show utility in phylogeny estimation. Furthermore, two different geographic locations of T. m. melloi and T. m. streptopeliae were analyzed to detect variation between populations. Molecular data revealed the existence of two distinct groups in the genus Tinaminyssus parasitic on columbiform birds. These results are in agreement with those reported by some authors using morphological characters. Sternostoma turdi parasitizing aerial birds appeared to be phylogenetically separated from other species of this genus isolated from aquatic birds. Moreover, our study addresses the validity of the subspecies status of T. melloi streptopeliae. This region of the mitochondrial 16S rDNA gene is a useful marker for inferring phylogenetic relationships among closely related rhinonyssid species, but not for more distantly related taxa.