RESUMO
BACKGROUND The aim of this study was to explore the effect and mechanism of tanshinone II A on proliferation, apoptosis, and migration of human colon cancer cells. MATERIAL AND METHODS CCK-8 approach was carried out to evaluate proliferation after applying various levels of tanshinone II A to SW620 colon carcinoma cells. Flow cytometry (FC) was used to assess apoptosis. Transwell assay was performed to assess invasion in vitro, and the wound-healing assay was applied to assess migration. Western blot analysis was performed to evaluate translation of mTOR, while RT-PCR was carried out to assess transcription of VEGF. RESULTS CCK-8 assay showed that tanshinone II A inhibited SW620 proliferation in comparison to the control group subsequent to 24 h, 48 h, and 72 h (P<0.001). FC revealed that tanshinone II A promoted SW620 apoptosis (P<0.001). The cell migration test revealed that the migration index of cells receiving tanshinone II A decreased. mTOR translation as well as VEGE transcription in cells receiving tanshinone II A was noticeably prohibited compared to control group (P<0.001). CONCLUSIONS Tanshinone II A is able to inhibit proliferation and migration of human colon cancer SW620 cells and promoted cell death. Its mechanism may be by downregulation of mTOR protein and VEGF mRNA.
Assuntos
Abietanos/farmacologia , Neoplasias do Colo/metabolismo , Abietanos/metabolismo , Antineoplásicos Fitogênicos/farmacologia , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Regulação para Baixo/efeitos dos fármacos , Humanos , Transdução de Sinais/efeitos dos fármacosRESUMO
OBJECTIVE: To search for an effective method for treatment of ankylosing spondylitis (AS). METHODS: The treatment group (n = 32) were treated by acupuncture combined with cupping therapy and the control group (n = 30) by simple acupuncture for 40 days. RESULTS: The clinical remission rate was 62.5% in the treatment group and 33.3% in the control group with a significant difference between the two groups (P<0.01); the total effective rate in the treatment group was 93.8% which was better than 83.3% in the control group (P<0.01); the recurrence rate after one year was 3.3% in the treatment group and 24.0% in the control group with significant difference between the two groups (P<0.01). CONCLUSION: Acupuncture combined with cupping therapy in the therapeutic effect on ankylosing spondylitis is better than simple acupuncture, with shorter therapeutic course and lower recurrence rate.