Effect of allelopathy on plant performance: a meta-analysis.

Allelopathy (i.e. chemical interactions between plants) is known to affect individual performance, community structure and plant invasions. Yet, a quantitative synthesis is lacking. Here, we performed a meta-analysis of 384 studies that measured allelopathic effects of one species (allelopathy plant) on another species or itself (test plant). Overall, allelopathy reduced plant performance by 25%, but the variation in allelopathy was high. The type of method affected the allelopathic effect: compared to leachates, allelopathy was more negative when residues of allelopathy plants were applied, and less negative when soil conditioned by allelopathy plants was applied. The negative effects of allelopathy diminished with study duration, and increased with concentrations of leachates or residues. Although allelopathy was not significantly related to lifespan, life form or domestication of the interacting plants, it became more negative with increasing phylogenetic distance. Moreover, native plants suffered more from leachates of naturalised alien plants than from leachates of other native plants. Our synthesis reveals that allelopathy could contribute to success of alien plants. The negative relationship between phylogenetic distance and allelopathy indicates that allelopathy might contribute to coexistence of closely related species (i.e. convergence) or dominance of single species.

Global exchange and accumulation of non-native plants.

All around the globe, humans have greatly altered the abiotic and biotic environment with ever-increasing speed. One defining feature of the Anthropocene epoch is the erosion of biogeographical barriers by human-mediated dispersal of species into new regions, where they can naturalize and cause ecological, economic and social damage. So far, no comprehensive analysis of the global accumulation and exchange of alien plant species between continents has been performed, primarily because of a lack of data. Here we bridge this knowledge gap by using a unique global database on the occurrences of naturalized alien plant species in 481 mainland and 362 island regions. In total, 13,168 plant species, corresponding to 3.9% of the extant global vascular flora, or approximately the size of the native European flora, have become naturalized somewhere on the globe as a result of human activity. North America has accumulated the largest number of naturalized species, whereas the Pacific Islands show the fastest increase in species numbers with respect to their land area. Continents in the Northern Hemisphere have been the major donors of naturalized alien species to all other continents. Our results quantify for the first time the extent of plant naturalizations worldwide, and illustrate the urgent need for globally integrated efforts to control, manage and understand the spread of alien species.

A dedicated eight-channel receive RF coil array for monkey brain MRI at 9.4 T.

The neuroimaging of nonhuman primates (NHPs) realised with magnetic resonance imaging (MRI) plays an important role in understanding brain structures and functions, as well as neurodegenerative diseases and pathological disorders. Theoretically, an ultrahigh field MRI (≥7 T) is capable of providing a higher signal-to-noise ratio (SNR) for better resolution; however, the lack of appropriate radiofrequency (RF) coils for 9.4 T monkey MRI undermines the benefits provided by a higher field strength. In particular, the standard volume birdcage coil at 9.4 T generates typical destructive interferences in the periphery of the brain, which reduces the SNR in the neuroscience-focused cortex region. Also, the standard birdcage coil is not capable of performing parallel imaging. Consequently, extended scan durations may cause unnecessary damage due to overlong anaesthesia. In this work, assisted by numerical simulations, an eight-channel receive RF coil array was specially designed and manufactured for imaging NHPs at 9.4 T. The structure and geometry of the proposed receive array was optimised with numerical simulations, so that the SNR enhancement region was particularly focused on monkey brain. Validated with rhesus monkey and cynomolgus monkey brain images acquired from a 9.4 T MRI scanner, the proposed receive array outperformed standard birdcage coil with higher SNR, mean diffusivity and fractional anisotropy values, as well as providing better capability for parallel imaging.

A numerical and experimental study of RF shimming in the presence of hip prostheses using adaptive SAR at 3 T.

PURPOSE: Parallel transmission techniques in MRI have the potential to improve the image quality near metal implants at 3 T. However, current testing of implants only evaluates the risk of radiofrequency (RF) heating in phantoms in circularly polarized mode. We investigate the influence of changing the transmission settings in a 2-channel body coil on the peak temperature near 2 CoCrMo hip prostheses, using adaptive specific absorption rate (SAR) as an estimate of RF heating. METHODS: Adaptive SAR is a SAR averaging method that is optimized to correlate with thermal simulations and limit the temperature to 39°C near hip implants. The simulated peak temperature was compared when using whole-body SAR, SAR10g , and adaptive SAR as a constraint for the maximum allowed input power. Adaptive SAR was used as a fast estimate of temperature to evaluate the trade-off between good image quality and low heating near the hip implants. Electromagnetic simulations were validated by simulating and measuring B1 maps and electric fields in a phantom at 3 T. RESULTS: Simulations and measurements showed excellent agreement. Limiting whole-body SAR to 2 W/kg and SAR10g to 10 W/kg resulted in temperatures up to 49.3°C and 40.7°C near the hip implants after 30 minutes of RF exposure, respectively. Predictions based on adaptive SAR limited the temperature to 39°C, and allowed to improve the B1 field distribution while preventing peak temperatures near the hip implants. CONCLUSION: Significant RF heating can occur at 3 T near hip implants when parallel transmission is used. Adaptive SAR can be integrated in RF shimming algorithms to improve the uniformity and reduce heating.

An open 8-channel parallel transmission coil for static and dynamic 7T MRI of the knee and ankle joints at multiple postures.

PURPOSE: We present the initial in vivo imaging results of an open architecture eight-channel parallel transmission (pTx) transceive radiofrequency (RF) coil array that was designed and constructed for static and dynamic 7T MRI of the knee and ankle joints. METHODS: The pTx coil has a U-shaped dual-row configuration (200 mm overall length longitudinally) that allows static and dynamic imaging of the knee and ankle joints at various postures and during active movements. This coil structure, in combination with B1 shimming, allows flexible configuration of B1 transmit profiles, with good homogeneity over 120-mm regions of interest. This coil enabled high-resolution gradient echo (e.g., 3D dual-echo steady state [DESS] and 3D multiecho data image combination [MEDIC]) and turbo spin echo (TSE) imaging (e.g., with proton density weighting [PDw], PDw with fat saturation, and T1 and T2 weightings) with local RF energy absorption rates well below regulatory limits. RESULTS: High-resolution 2D and 3D image series (e.g., 0.3 mm in-plane resolution for TSE, 0.47 mm isotropic for DESS and MEDIC) were obtained from the knee and ankle joints with excellent tissue contrast. Dynamic imaging during continuous knee and ankle flexion-extension cycles were successfully acquired. CONCLUSION: The new open pTx coil array provides versatility for high-quality static and dynamic MRI of the knee and ankle joints at 7T. Magn Reson Med 79:1804-1816, 2018. © 2017 International Society for Magnetic Resonance in Medicine.

Radial magnetic resonance imaging (MRI) using a rotating radiofrequency (RF) coil at 9.4 T.

The rotating radiofrequency coil (RRFC) has been developed recently as an alternative approach to multi-channel phased-array coils. The single-element RRFC avoids inter-channel coupling and allows a larger coil element with better B1 field penetration when compared with an array counterpart. However, dedicated image reconstruction algorithms require accurate estimation of temporally varying coil sensitivities to remove artefacts caused by coil rotation. Various methods have been developed to estimate unknown sensitivity profiles from a few experimentally measured sensitivity maps, but these methods become problematic when the RRFC is used as a transceiver coil. In this work, a novel and practical radial encoding method is introduced for the RRFC to facilitate image reconstruction without the measurement or estimation of rotation-dependent sensitivity profiles. Theoretical analyses suggest that the rotation-dependent sensitivities of the RRFC can be used to create a uniform profile with careful choice of sampling positions and imaging parameters. To test this new imaging method, dedicated electronics were designed and built to control the RRFC speed and hence positions in synchrony with imaging parameters. High-quality phantom and animal images acquired on a 9.4 T pre-clinical scanner demonstrate the feasibility and potential of this new RRFC method.

Niche dynamics of alien species do not differ among sexual and apomictic flowering plants.

Biological invasions can be associated with shifts of the species' climatic niches but the incidence of such shifts is under debate. The reproductive system might be a key factor controlling such shifts because it influences a species' evolutionary flexibility. However, the link between reproductive systems and niche dynamics in plant invasions has been little studied so far. We compiled global occurrence data sets of 13 congeneric sexual and apomictic species pairs, and used principal components analysis (PCA) and kernel smoothers to compare changes in climatic niche optima, breadths and unfilling/expansion between native and alien ranges. Niche change metrics were compared between sexual and apomictic species. All 26 species showed changes in niche optima and/or breadth and 14 species significantly expanded their climatic niches. However, we found no effect of the reproductive system on niche dynamics. Instead, species with narrower native niches showed higher rates of niche expansion in the alien ranges. Our results suggest that niche shifts are frequent in plant invasions but evolutionary potential may not be of major importance for such shifts. Niche dynamics rather appear to be driven by changes of the realized niche without adaptive change of the fundamental climatic niche.

Global trade will accelerate plant invasions in emerging economies under climate change.

Trade plays a key role in the spread of alien species and has arguably contributed to the recent enormous acceleration of biological invasions, thus homogenizing biotas worldwide. Combining data on 60-year trends of bilateral trade, as well as on biodiversity and climate, we modeled the global spread of plant species among 147 countries. The model results were compared with a recently compiled unique global data set on numbers of naturalized alien vascular plant species representing the most comprehensive collection of naturalized plant distributions currently available. The model identifies major source regions, introduction routes, and hot spots of plant invasions that agree well with observed naturalized plant numbers. In contrast to common knowledge, we show that the 'imperialist dogma,' stating that Europe has been a net exporter of naturalized plants since colonial times, does not hold for the past 60 years, when more naturalized plants were being imported to than exported from Europe. Our results highlight that the current distribution of naturalized plants is best predicted by socioeconomic activities 20 years ago. We took advantage of the observed time lag and used trade developments until recent times to predict naturalized plant trajectories for the next two decades. This shows that particularly strong increases in naturalized plant numbers are expected in the next 20 years for emerging economies in megadiverse regions. The interaction with predicted future climate change will increase invasions in northern temperate countries and reduce them in tropical and (sub)tropical regions, yet not by enough to cancel out the trade-related increase.

Short wind pulses consistently change the morphology of roots, but not of shoots, across young plants of different growth forms.

Wind is an environmental stimulus that stresses plants of all growth forms at all life-stages by influencing the development, architecture, and morphology of roots and shoots. However, comparative studies are scarce and no study directly investigated whether shoot and root morphological traits of trees, grasses and forbs differ in their response to short wind pulses of different wind intensity. In this study, we found that across species, wind stress by short wind pulses of increasing intensity consistently changed root morphology, but did not affect shoot morphological traits, except plant height in four species. Wind effects in roots were generally weak in tree species but consistent across growth forms. Furthermore, plant height of species was correlated with changes in specific root length and average diameter.Our results indicate that short-pulse wind treatments affect root morphology more than shoot morphology across growth forms. They further suggest that wind stress possibly promotes root anchorage in young plants and that these effects might depend on plant height.

An orthogonal-based decoupling method for MRI phased array coil design.

A new 2 T 3-element orthogonal knee coil array based on the three-dimensional orthogonality principle was designed, constructed and used in a series of pilot magnetic resonance imaging (MRI) studies on a standardized phantom, and human and pig knees. The coil elements within this new coil array are positioned orthogonal to one another allowing problematic mutual coupling effects to be minimized without the use of any passive mutual decoupling schemes. The proposed method is appropriate for the design of transmit, receive and/or transceive radiofrequency (RF) coil arrays for applications in animal/human MRI and spectroscopic studies. Experimental results demonstrated that the 3-element orthogonal knee coil array could be angled arbitrarily, including at 90°, relative to the main static magnetic field (B(0) ) whilst maintaining normal operation with minimal loss of efficiency and functionality. Initial trials with a pig knee specimen further showed that the greatest signal intensity in the patellar ligament (parallel collagen fibres) was observed when the orthogonal knee coil array and the pig knee specimen were angled at ~55° to B(0) , which may have potential uses in magic angle MR applications.

Integrated Multi-Modal Antenna With Coupled Radiating Structures (I-MARS) for 7T pTx Body MRI.

One of the main challenges in ultra-high field whole body MRI relates to the uniformity and efficiency of the radiofrequency field. Although recent advances in the design of RF coils have demonstrated that dipole antennas have a current distribution ideally suited to 7T MRI, they are limited by low isolation and poor robustness to loading changes. Multi-layered and self-decoupled loop coils have demonstrated improved RF performance in these areas at lower field MRI but have not been adapted to dipole designs. In this work, we introduce a novel type of RF antenna consisting of integrated multi-modal antenna with coupled radiating structures (I-MARS), which use layered conductors and dielectric substrates to allow dipole and transmission line modes to co-exist on the same compact dipole-shaped structure. The proposed antenna was optimally designed for 7T MRI and compared with existing dipole antennas using numerical simulations, which showed that I-MARS had similar B1 over specific absorption rate efficiency and superior isolation and stability. Subsequently, a prototype pTx coil array was built and tested in vivo on healthy volunteers at 7T. The articulated, modular construction of the I-MARS coil array allowed it to be readily conformed across multiple body regions (hip, knee, shoulder, lumbar spine and prostate), without requiring modification of the tuning and matching of the antennas. Using RF shimming, uniform and efficient excitation was successfully achieved in the acquisition of high-resolution MR images.

Altitudinal distribution patterns of the native and alien woody flora in Kashmir Himalaya, India.

BACKGROUND: Many studies have shown that alien species richness pattern follows that of native species richness patterns along environmental gradients, without taking the specific composition of the two groups into account. OBJECTIVES: To compare species richness patterns of native and alien woody plants along an altitudinal gradient in Kashmir Himalaya, India, and to analyse the specific composition, e.g. proportion of life forms. METHODS: Analysis of secondary data from published floristic inventories. The gradient (500-4800m asl) was split into 100m bands and presence/absence data for each species were obtained, for each band. RESULTS: Species richness of both native and alien species followed a hump-shaped distribution. Alien species richness dropped faster above 2000masl than the native did. The ratio of trees to shrubs decreased monotonically along the gradient in native species, but showed a peak at c. 2500masl in alien species. Alien species flowered in average earlier than native species. CONCLUSIONS: The change of species richness of native and alien species along altitude is similar, but the proportion of life forms is not. Most likely both climatic and socio-economic factors affect alien species richness and its specific composition in the Kashmir Himalaya.

Image reconstruction for the rotating RF coil using k-t bin robust principal component analysis (RPCA) method.

The recently developed rotating radiofrequency coil (RRFC) technique has been proven to be an alternative solution to phased-array coils for magnetic resonance imaging (MRI). However, most of the image reconstruction methods for the RRFC requires detailed knowledge of coil sensitivity to yield optimal results. In this work, a novel reconstruction algorithm based on Robust Principal Component Analysis (RPCA) with the k-t (k-space-time domain) sparse bin reformation method (or rotating k-t bin method) has been presented to restore images without using dedicated coil sensitivity information. The proposed algorithm recovers images by iteratively removing the artefacts in both temporal and frequency domains caused by the Fourier invariant violation from coil rotation. The data sampling scheme consists of the golden angle (GA) radial k-space and the stepping-mode coil rotation. Simulation results demonstrate the effectiveness of the proposed imaging method for the RRFC-based MR scan.

Metamaterial-Inspired Radiofrequency (RF) Shield With Reduced Specific Absorption Rate (SAR) and Improved Transmit Efficiency for UHF MRI.

To prevent the interferences between radiofrequency (RF) coils and other components in the magnetic resonance imaging (MRI) system such as gradient coils, it is essential to place an RF shield between the RF coils and gradient coils. However, the induced currents on conventional RF shields have negative influences on the RF coil performance. To reduce these influences, metamaterial absorbers (MA), a class of metamaterials exhibiting nearly unity absorption rate for the incident electromagnetic fields, can be employed for the design of a novel RF shield. However, the adoption of metamaterials in MRI systems is usually problematic because of the bulkiness of the metamaterial structure. In this work, capacitors and metallic interconnectors are used to miniaturize the MA so that the unit MA cell can operate at the Larmor frequencies of 7T and 9.4T MRI and stay compact. This MA-RF shield is used to improve the transmit efficiency of RF surface coils and reduce the specific absorption rate (SAR) in the region of interest (ROI). It is successfully demonstrated by simulations and experiments that, compared with conventional RF shield structure, the transmit efficiency can be enhanced by more than 32% and the peak SAR value can be reduced by 22% using the MA-RF shield. Moreover, it is observed that the transmit field penetration is improved when the surface coil is used with the MA-RF shield. This proof-of-concept study suggests a new practical way for the utilization of metamaterials in ultra-high field MRI applications.

A meta-analysis of trait differences between invasive and non-invasive plant species.

A major aim in ecology is identifying determinants of invasiveness. We performed a meta-analysis of 117 field or experimental-garden studies that measured pair-wise trait differences of a total of 125 invasive and 196 non-invasive plant species in the invasive range of the invasive species. We tested whether invasiveness is associated with performance-related traits (physiology, leaf-area allocation, shoot allocation, growth rate, size and fitness), and whether such associations depend on type of study and on biogeographical or biological factors. Overall, invasive species had significantly higher values than non-invasive species for all six trait categories. More trait differences were significant for invasive vs. native comparisons than for invasive vs. non-invasive alien comparisons. Moreover, for comparisons between invasive species and native species that themselves are invasive elsewhere, no trait differences were significant. Differences in physiology and growth rate were larger in tropical regions than in temperate regions. Trait differences did not depend on whether the invasive alien species originates from Europe, nor did they depend on the test environment. We conclude that invasive alien species had higher values for those traits related to performance than non-invasive species. This suggests that it might become possible to predict future plant invasions from species traits.

Wind intensity affects fine root morphological traits with consequences for plant-soil feedback effects.

Wind influences the development, architecture and morphology of plant roots and may modify subsequent interactions between plants and soil (plant-soil feedbacks-PSFs). However, information on wind effects on fine root morphology is scarce and the extent to which wind changes plant-soil interactions remains unclear. Therefore, we investigated the effects of two wind intensity levels by manipulating surrounding vegetation height in a grassland PSF field experiment. We grew four common plant species (two grasses and two non-leguminous forbs) with soil biota either previously conditioned by these or other species and tested the effect of wind on root:shoot ratio, fine root morphological traits as well as the outcome for PSFs. Wind intensity did not affect biomass allocation (i.e. root:shoot ratio) in any species. However, fine-root morphology of all species changed under high wind intensity. High wind intensity increased specific root length and surface area and decreased root tissue density, especially in the two grasses. Similarly, the direction of PSFs changed under high wind intensity in all four species, but differences in biomass production on the different soils between high and low wind intensity were marginal and most pronounced when comparing grasses with forbs. Because soils did not differ in plant-available nor total nutrient content, the results suggest that wind-induced changes in root morphology have the potential to influence plant-soil interactions. Linking wind-induced changes in fine-root morphology to effects on PSF improves our understanding of plant-soil interactions under changing environmental conditions.

Geometric distortion characterization and correction for the 1.0 T Australian MRI-linac system using an inverse electromagnetic method.

PURPOSE: The magnetic resonance imaging (MRI)-Linac system combines a MRI scanner and a linear accelerator (Linac) to realize real-time localization and adaptive radiotherapy for tumors. Given that the Australian MRI-Linac system has a 30-cm diameter of spherical volume (DSV) with a shimmed homogeneity of ±4.05 parts per million (ppm), a gradient nonlinearity (GNL) of <5% can only be assured within 15 cm from the system's isocenter. GNL increases from the isocenter and escalates close to and outside of the edge of the DSV. Gradient nonlinearity can cause large geometric distortions, which may provide inaccurate tumor localization and potentially degrade the radiotherapy treatment. In this study, we aimed to characterize and correct the geometric distortions both inside and outside of the DSV. METHODS: On the basis of phantom measurements, an inverse electromagnetic (EM) method was developed to reconstitute the virtual current density distribution that could generate gradient fields. The obtained virtual EM source was capable of characterizing the GNL field both inside and outside of the DSV. With the use of this GNL field information, our recently developed "GNL-encoding" reconstruction method was applied to correct the distortions implemented in the k-space domain. RESULTS: Both phantom and in vivo human images were used to validate the proposed method. The results showed that the maximal displacements within an imaging volume of 30 cm × 30 cm × 30 cm after using the fifth-order spherical harmonic (SH) method and the proposed method were 6.1 ± 0.6 mm and 1.8 ± 0.6 mm, respectively. Compared with the fifth-order SH-based method, the new solution decreased the percentage of markers (within an imaging volume of 30 cm × 30 cm × 30 cm) with ≥1.5-mm distortions from 6.3% to 1.3%, indicating substantially improved geometric accuracy. CONCLUSIONS: The experimental results indicated that the proposed method could provide substantially improved geometric accuracy for the region outside of the DSV, when comparing with the fifth-order SH-based method.

Hybrid numerical techniques for the modelling of radiofrequency coils in MRI.

Radiofrequency (RF) coils for use in MRI can have a significant effect on both the signal-to-noise-ratio of MR images and the specific absorption rate inside the biological sample. In the past, prototypes were constructed and tested to investigate the performance of the RF coils and often required several iterations to achieve an acceptable result. However, with the advancement in computational electromagnetic techniques, RF coil modelling has now become the modus operandi of coil design because it can produce accurate numerical results, thus reducing the time and effort spent in designing and prototyping RF coils. Two hybrid methods -method of moments (MoM)/finite difference time domain (FDTD) and MoM/finite element method (FEM) - for RF coil modelling are presented herein. The paper provides a brief overview of FDTD, FEM and MoM. It discusses the hybridisation of these methods and how they are integrated to form versatile techniques. The numerical results obtained from these hybrid methods are compared with experimental results from prototype coils over a range of operating frequencies. The methods are then applied to the design of a new type of phased-array coil - the rotary phased array. From these comparisons, it can be seen that the numerical methods provide a useful aid for the design and optimisation of RF coils for use in MRI.

Monopole antenna array design for 3 T and 7 T magnetic resonance imaging.

PURPOSE: Ultra-high field magnetic resonance imaging poses a number of challenges for robust radio frequency coil designs. A monopole antenna array can potentially overcome key limitations of birdcage coil designs and may provide a useful radio frequency coil for brain imaging. METHODS: Four, 8 and 12 element monopole antenna arrays were simulated using 3 T and 7T magnetic resonance imaging frequencies. For comparison, 4, 8 and 12 element birdcage coils were also simulated. Coil performance was evaluated and compared and the impact of shielding was assessed. A 4 element monopole antenna array was fabricated and bench tested. RESULTS: Comparison of the 4, 8 and 12 element designs suggest that the monopole antenna array leads to better field properties than the birdcage coil in all configurations studied: unloaded, loaded with saline and loaded using a head phantom. Improvements in field properties and homogeneity were evident at both field strengths, implying that the monopole antenna array has potential for head imaging. The monopole antenna array also appears to be more efficient than the comparable birdcage coil design. Additionally, the former is scalable via the addition of more elements whereas our results suggest that this is not the case for the latter. Bench testing results show that the monopole antenna array is well matched with the transmission line, and mutual coupling between elements is sufficiently low. CONCLUSION: We found the monopole antenna array generated a larger field intensity than the birdcage coil design, whilst also producing a more useful magnetic resonance imaging field as measured by radio frequency field homogeneity. Our study suggests that magnetic resonance imaging of the brain can likely benefit from the use of radio frequency monopole antenna arrays.

Drivers of the relative richness of naturalized and invasive plant species on Earth.

Biological invasions are a defining feature of the Anthropocene, but the factors that determine the spatially uneven distribution of alien plant species are still poorly understood. Here, we present the first global analysis of the effects of biogeographic factors, the physical environment and socio-economy on the richness of naturalized and invasive alien plants. We used generalized linear mixed-effects models and variation partitioning to disentangle the relative importance of individual factors, and, more broadly, of biogeography, physical environment and socio-economy. As measures of the magnitude of permanent anthropogenic additions to the regional species pool and of species with negative environmental impacts, we calculated the relative richness of naturalized (= RRN) and invasive (= RRI) alien plant species numbers adjusted for the number of native species in 838 terrestrial regions. Socio-economic factors (per-capita gross domestic product (GDP), population density, proportion of agricultural land) were more important in explaining RRI (~50 % of the explained variation) than RRN (~40 %). Warm-temperate and (sub)tropical regions have higher RRN than tropical or cooler regions. We found that socio-economic pressures are more relevant for invasive than for naturalized species richness. The expectation that the southern hemisphere is more invaded than the northern hemisphere was confirmed only for RRN on islands, but not for mainland regions nor for RRI. On average, islands have ~6-fold RRN, and >3-fold RRI compared to mainland regions. Eighty-two islands (=26 % of all islands) harbour more naturalized alien than native plants. Our findings challenge the widely held expectation that socio-economic pressures are more relevant for plant naturalization than for invasive plants. To meet international biodiversity targets and halt the detrimental consequences of plant invasions, it is essential to disrupt the connection between socio-economic development and plant invasions by improving pathway management, early detection and rapid response.