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1.
J Sports Sci ; 35(5): 484-490, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27071002

RESUMO

This study examined whether or not activity monitor data collected as part of a typical 7-day physical activity (PA) measurement protocol can be expected to be missing at random. A total of 315 participants (9-18 years) each wore a SenseWear Armband monitor for 7 consecutive days. Participants were classified as "compliant" (86 boys and 124 girls) if they had recorded accelerometer data during 70% or more of the predefined awake time (7 AM-10 PM) on four different days; and "non-compliant" (44 boys and 51 girls) when not meeting these criteria. Linear mixed models were used to examine differences in energy expenditure (EE) levels by compliance across 10 different time periods. The results indicated that non-compliant girls were older (13.4 ± 2.9 vs. 12.2 ± 2.5) and taller (156.8 ± 10.3 vs. 152.8 ± 11.3) than their same gender compliant peers (P < .05). Comparisons of EE rates at segmented portions of the day revealed no differences between compliant and non-compliant groups (P ≥ .05). Differences in EE ranged from -0.32 kcal · kg-1 · h-1 (before school time) to 0.62 kcal · kg-1 · h-1 (physical education class) in boys and -0.39 kcal · kg-1 · h-1 (transportation from school) to 0.37 kcal · kg-1 · hour-1 (recess) in girls. The results showed that compliant and non-compliant individuals differed in a few demographic characteristics but exhibited similar activity patterns. This suggests that data were considered to be missing at random, but additional work is needed to confirm this observation in a representative sample of children using other types of activity monitors and protocols.


Assuntos
Acelerometria/normas , Exercício Físico , Comportamentos Relacionados com a Saúde , Monitorização Fisiológica/normas , Adolescente , Criança , Metabolismo Energético , Feminino , Humanos , Masculino
2.
J Am Coll Health ; : 1-8, 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36595662

RESUMO

Objective: Health coaching (HC) may promote healthy behaviors in incoming college students. Participants: 187 undergraduate students (76% female; age 18.6 ± 1.6) were recruited to participate in a peer HC program. Methods: Participants received four HC sessions in eight-weeks to work toward self-selected goals of physical activity (PA), diet, or stress management. PA was assessed using the International Physical Activity Questionnaire Short-Form, diet with the Eating Habits Confidence Survey, and stress with the Perceived Stress Scale. Results: Repeated measures ANOVAs investigated lifestyle behavior changes in 130 participants. Significant effects of time were reported, with a 39.6% gain in PA, 9.9% increase in dietary habit, and 16.3% decline in stress levels with moderate to large effect sizes (ηp2: 0.07-0.17; p< 0.01). Conclusion: The peer HC model promoted healthy lifestyles in college students. Additional research is needed to understand the effects of HC specifically for each goal, and student reactions to peer-led HC.

3.
Scand J Med Sci Sports ; 21(2): 310-7, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-20030783

RESUMO

The aim of this study was to examine the associations between objectively assessed physical activity (PA) and muscular fitness (MF) in adolescents, and to determine whether only resistance training exercise is associated with the increase of MF. A sample of 211 Spanish adolescents (105 girls) wore the ActiGraph monitor for 7 days. Participants also completed a fitness evaluation including three MF tests (handgrip strength, a 60-s abdominal test and a standing broad jump), a cardiorespiratory fitness assessment and anthropometric measures. A standardized MF score was computed using the three muscular tests. Adolescent participation in resistance training was self-reported. Linear regression showed that only vigorous PA was significantly (P=0.041) and positively (ß=0.133) associated with MF after adjustment by gender, age, pubertal status, BMI, and cardiorespiratory fitness. Significant differences in MF were found between youth in the lowest and upper tertiles of vigorous PA. Moreover, adolescents who were involved in resistance training had significantly higher MF scores compared with youth in low and medium tertiles of vigorous PA, but no significant differences were found when compared with non-lifters from the highest tertile of vigorous PA (P=0.356). These findings suggest that vigorous activity may be associated with indicators of MF in adolescents.


Assuntos
Atividade Motora/fisiologia , Músculo Esquelético/fisiologia , Aptidão Física/fisiologia , Treinamento Resistido , Adolescente , Índice de Massa Corporal , Feminino , Humanos , Modelos Lineares , Masculino , Força Muscular/fisiologia , Resistência Física/fisiologia , Espanha
4.
Nutr Hosp ; 24(2): 226-32, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19593496

RESUMO

BACKGROUND: It is necessary to know the levels of physical activity (PA) for a better understanding of the development of chronic diseases in youth. The aim of this study was to assess levels of total PA and time spent in different PAintensities in Spanish adolescents by accelerometer. METHODS: A sub-sample of 214 healthy Spanish adolescents (107 females), aged 13-16 years, enrolled in the AFINOS Study was selected for this study. Participants wore the ActiGraph GT1M accelerometer for 7 consecutive days. Total PA and time spent in sedentary, light, moderate, vigorous, and moderate to vigorous physical activity (MVPA) was estimated using the accelerometer. Adiposity was measured in the sample using sum of 6 skinfoldthickness, waist circumference and BMI. RESULTS: Adolescent boys were engaged in higher levels of total PA, moderate PA, vigorous PA and MVPA than adolescent girls, whereas girls were engaged in higher levels of light PA. Differences between age groups showed that the 15-16 years group did more total PA (P = 0.008) than the 13-14 years group. Adolescents with highest levels of body fat were less active and spent less time in vigorous PA and MVPA than adolescents with less body fat. Among the current sample, 71.1% of the adolescents (82.2% adolescent boys and 60.7% adolescent girls) reached the recommendation of > or = 60 min in MVPA. CONCLUSIONS: Although these findings suggest that Spanish adolescents have similar PA levels than other European adolescents, further cross-sectional and longitudinal studies must assess PA levels in free-living conditions in Spanish children and adolescents using objective methods such as accelerometers, heart rate monitors and pedometers.


Assuntos
Monitorização Ambulatorial , Atividade Motora , Adolescente , Feminino , Humanos , Masculino , Monitorização Ambulatorial/instrumentação , Espanha
5.
Nutr Hosp ; 24(6): 701-10, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-20049374

RESUMO

INTRODUCTION: The aim of this study was to evaluate the agreement between the ActiGraph accelerometer and the Bouchard diary to estimate energy expenditure (EE) in a Spanish adolescent population. METHODS: Sixty-one Spanish adolescents, aged 12-16 years, were recruited for this study. The Bouchard diary and the ActiGraph were administered for 3 consecutive days. EE estimated by the Bouchard diary was calculated using 2 different MET category values (BD-Bouchard and BD-Bratteby). EE estimated by the ActiGraph was calculated using 3 predictive equations (AC-Trost, AC-Freedson, and AC-Ekelund). Participants with complete 24 h data were also analyzed to control the possible loss of accuracy. Agreement was examined by Pearson and concordance correlations, paired t-test, and Bland-Altman method. RESULTS: Thirty-seven adolescents were included in the analyses with complete 72 h. Relationships between EE calculated by the Bouchard diary and the ActiGraph were high (ranged: r = 0.61-0.78). Concordance correlations were moderate (r c = 0.60) by BD-Bratteby and AC-Trost, and low using BD-Bratteby and AC-Ekelund (r c = 0.15). EE estimated by BD-Bratteby and AC-Trost also showed no significant differences (P> 0.05) and absolutely agree 0.0 +/- 5.0 MJ/d (95% confidence interval: +/- 0.90 MJ/d), but with wide limits of agreement (+/- 9.80 MJ/d). Relationships were higher and differences were smaller in the 72 h sample than in the 24 h sample. CONCLUSIONS: The Bouchard diary and the ActiGraph showed high relationships, moderate concordance, and large differences to estimate EE in Spanish adolescents. Advantages, disadvantages, and agreements between both instruments must be taking into consideration for health-related research.


Assuntos
Actigrafia/métodos , Metabolismo Energético , Prontuários Médicos , Monitorização Ambulatorial/métodos , Actigrafia/instrumentação , Adolescente , Viés , Criança , Feminino , Atividades Humanas , Humanos , Masculino , Monitorização Ambulatorial/instrumentação , Atividade Motora , Valores de Referência , Reprodutibilidade dos Testes , Espanha
6.
Obes Sci Pract ; 5(3): 220-230, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31275595

RESUMO

OBJECTIVE: Integration of behavioural risk assessment into well-child visits is recommended by clinical guidelines, but its feasibility and impact is unknown. METHODS: A quasi-experimental study evaluated the feasibility and effectiveness of risk assessment on body mass index (BMI) at 1-year follow-up. Children with assessments (intervention) were compared with those who did not complete assessments (non-respondent) and those who received standard care (non-exposed). RESULTS: Analyses included 10,647 children aged 2-9 years (2,724 intervention, 3,324 non-respondent and 4,599 non-exposed). Forty-five per cent of parents completed the assessments. Intervention and non-respondent groups differed in change in BMI z-score at 1 year by -0.05 (confidence interval [CI]: -0.08, -0.02; P = 0.0013); no difference was observed with non-exposed children. The intervention group had a smaller increase in BMI z-score (0.07 ± 0.63) than non-respondent group (0.13 ± 0.63). For children with normal weight at baseline, intervention versus non-respondent groups differed in BMI z-score change by -0.06 (CI: -0.10, -0.02; P = 0.0025). However, children with overweight at baseline in the intervention versus the non-exposed group differed in BMI z-score change (0.07 [CI: 0.02, 0.14]; P = 0.016). When analysed by age, results were similar for 2- to 5-year-olds, but no differences were found for 6- to 9-year-olds. CONCLUSION: Automating risk assessment in paediatric care is feasible and effective in promoting healthy weight among preschool but not older children.

7.
Int J Obes (Lond) ; 32(4): 613-8, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18209737

RESUMO

OBJECTIVE: The purpose of this study was to examine the combined influence of physical activity (PA) and television viewing (TV) on the risk of overweight in US youth ages 14-18 years. RESEARCH DESIGN AND METHODS: Cross-sectional data from a nationally representative sample of approximately 13,600 US high school students participating in the 2001 Centers for Disease Control and Prevention Youth Risk Behavior Survey (YRBS) were examined. Participants were cross-tabulated into nine PA-TV groups according to the level of moderate (MPA) or vigorous PA (VPA) (low: < or = 2 days per week; moderate: 3-5 days per week; high: 6-7 days per week) and TV (low: < or = 1 h per day; moderate: 2-3 h per day; high: > or = 4 h per day). The referent group was the low TV/high PA group. The body mass index was used to determine overweight and obesity based on the International Obesity Task Force reference values. Logistic regression was used to examine the influence of TV and PA on the odds of overweight in boys and girls, while controlling for age and ethnicity. RESULTS: Boys and girls watching low levels of TV did not have increased odds of overweight regardless of PA level with the exception of girls with low TV/low VPA (odds ratio (OR)=1.48). Girls who watched moderate and high levels of TV had increased odds of overweight at any level of MPA or VPA (OR 1.24-3.11). In girls, the odds of overweight increased in a graded manner across PA levels for both the moderate and high levels of TV. Girls with high TV/low VPA had the highest odds of overweight (OR=3.11). In general, most of the associations were stronger in girls compared to boys. CONCLUSION: The results highlight the importance of considering both PA and TV as risk factors for overweight in adolescents.


Assuntos
Comportamento do Adolescente/fisiologia , Atividade Motora/fisiologia , Sobrepeso/fisiopatologia , Televisão/estatística & dados numéricos , Adolescente , Índice de Massa Corporal , Métodos Epidemiológicos , Exercício Físico/fisiologia , Feminino , Humanos , Masculino , Obesidade/epidemiologia , Obesidade/fisiopatologia , Sobrepeso/epidemiologia , Fatores Sexuais , Estados Unidos/epidemiologia
8.
Eur J Clin Nutr ; 69(7): 850-5, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25351651

RESUMO

BACKGROUND/OBJECTIVES: Objective and subjective measurement instruments have been used to estimate energy expenditure (EE) as alternatives to the doubly labeled water (DLW) methodology, but their relative validity for older adults remains uncertain. The purpose of this study was to validate an objective monitor (SenseWear Mini Armband) and a self-report instrument (7-Day Physical Activity Recall, 7D-PAR) relative to the DLW under free-living conditions in older adults. SUBJECTS/METHODS: Twenty-nine older adults (60-78 years) each wore the Mini for 14 consecutive days and completed two 7D-PARs after each week. For each measurement method, activity EE (AEE) was calculated as total EE (TEE)­measured resting metabolic rate (RMR)­diet induced thermogenesis (10% of TEE). TEE and AEE from the Mini and 7D-PAR were each compared with values from the DLW. RESULTS: Equivalence testing indicated that estimates of TEE from the Mini and the 7D-PAR were statistically equivalent to those measured with DLW; however, differences were evident for estimates of AEE. The Mini had smaller mean absolute percent error for TEE (8.0%) and AEE (28.4%) compared with the 7D-PAR (13.8 and 84.5%, respectively) and less systematic bias in the estimates. CONCLUSIONS: The Mini and 7D-PAR provided reasonably valid estimates of TEE but large errors in estimating AEE. The Mini and 7D-PAR have the potential to accurately estimate TEE for older adults.


Assuntos
Envelhecimento , Metabolismo Energético , Atividade Motora , Actigrafia , Idoso , Metabolismo Basal , Estudos de Coortes , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Reprodutibilidade dos Testes , Autorrelato , Termogênese
9.
Physiol Meas ; 36(5): 1037-46, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25903066

RESUMO

Assessing physical activity (PA) is a challenging task and many different approaches have been proposed. Direct observation (DO) techniques can objectively code both the behavior and the context in which it occurred, however, they have significant limitations such as the cost and burden associated with collecting and processing data. Therefore, this study evaluated the utility of an automated video analysis system (CAM) designed to record and discriminate the intensity of PA using a subject tracking methodology. The relative utility of the CAM system and DO were compared with criterion data from an objective accelerometry-based device (Actigraph GT3X+). Eight 10 year old children (three girls and five boys) wore the GT3X+ during a standard basketball session. PA was analyzed by two observers using the SOPLAY instrument and by the CAM system. The GT3X+ and the CAM were both set up to collect data at 30 Hz while the DO was performed every two minutes, with 10 s of observation for each gender. The GT3X+ was processed using cut points by Evanson and the outcome measure was the percentage of time spent in different intensities of PA. The CAM data were processed similarly using the same speed thresholds as were used in establishing the Evenson cut-off points (light: <2 mph; walking: 2-4 mph; very active: >4 mph). Similar outcomes were computed from the SOPLAY default analyses. A chi-square test was used to test differences in the percentage of time at the three intensity zones (light, walking and very active). The Yates' correction was used to prevent overestimation of statistical significance for small data. When compared with GT3X+, the CAM had better results than the SOPLAY. The chi-square test yielded the following pairwise comparisons: CAM versus GT3x+ was χ(2) (5) = 24.18, p < .001; SOPLAY2 versus GT3x+ was χ(2) (5) = 144.44, p < .001; SOPLAY1 versus GT3x+ was χ(2) (5) = 119.55, p < .001. The differences were smaller between CAM and GT3x+, suggesting that the video tracking system provided better agreement than DO. The small sample size precludes a definitive evaluation but the results show that the CAM video system may have promise for automated coding of physical activity behavior.


Assuntos
Atividade Motora , Gravação em Vídeo/métodos , Acelerometria , Criança , Feminino , Humanos , Masculino
10.
J Sports Med Phys Fitness ; 55(10): 1252-63, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25303074

RESUMO

AIM: The purpose of this study was to test utility of different fitness field tests to predict self-reported low-back pain (LBP) in youth. METHODS: A sample of 376 school aged (4th through 10th grade students) first completed a survey instrument designed to assess past events (and degree) of LBP. On subsequent days, participants completed a battery of different muscular fitness tests including the current FITNESSGRAM trunk extension test. Receiver operator characteristic curves (ROC) were used to test the utility of single and combined scores from various tests to predict self-reported LBP. RESULTS: The results show that the trunk extension test used in FITNESSGRAM has poor utility for predicting self-reported LBP. In girls, the sit-and-reach, the static curl-up test, and a composite score using static and dynamic curl-up scores had the highest Area Under the Curve (AUC=0.80, 0.71, and 0.79, respectively). Sensitivity (Se) values ranged from 75.0-85.7 while Specificity (Sp) ranged from 59.1-81.4. In boys, the dynamic curl-up test alone was the best predictor of LBP in high-school boys (AUC=0.75, Sensitivity=75.0 and Specificity=74.1). CONCLUSION: The reasonable Sensitivity values from the ROC analyses indicate that individual and aggregate indicators of musculoskeletal fitness can potentially identify girls that had LBP in the past. However, the low values for Specificity indicates that girls with "No LBP" can be misclassified as being at risk for LBP. The current FITNESSGRAM trunk extension test was not able to discriminate between students with and without LBP therefore, the utility of this test for fitness assessment in youth should be revisited.


Assuntos
Teste de Esforço/métodos , Dor Lombar/diagnóstico , Tronco/fisiologia , Adolescente , Criança , Teste de Esforço/normas , Feminino , Humanos , Masculino , Aptidão Física , Curva ROC , Reprodutibilidade dos Testes , Autorrelato , Inquéritos e Questionários
11.
FEBS Lett ; 257(1): 68-70, 1989 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-2509246

RESUMO

Astrocyte cultures prelabelled with [3H]inositol were exposed to arachidonic acid (AA) in the presence and absence of various agonists. AA alone evoked a dose-dependent increase in the accumulation of inositol phosphates (IP), an effect not secondary to eicosanoid synthesis and release but which was abolished by EGTA. Separation of the IP revealed that AA stimulated increases in inositol tris-, bis- and monophosphates. IP formation evoked by carbachol or norepinephrine was additive with AA, whereas IP formation by platelet activating factor (PAF) or ATP was non-additive with AA. These results suggest that AA released upon stimulation of astrocytes or other cells in the CNS could initiate and/or amplify intercellular signalling.


Assuntos
Ácidos Araquidônicos/farmacologia , Astrócitos/metabolismo , Fosfatos de Inositol/metabolismo , Animais , Animais Recém-Nascidos , Ácido Araquidônico , Astrócitos/efeitos dos fármacos , Carbacol/farmacologia , Células Cultivadas , Córtex Cerebral/metabolismo , Hidrólise , Inositol/metabolismo , Cinética , Norepinefrina/farmacologia , Fator de Ativação de Plaquetas/farmacologia , Ratos
12.
J Appl Physiol (1985) ; 70(4): 1463-8, 1991 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-2055822

RESUMO

It has been previously observed that the thermic effect of a glucose load is potentiated by prior exercise. To determine whether this phenomenon is observed when different carbohydrates are used and to ascertain the role of insulin, the thermic effects of fructose and glucose were compared during control (rest) and postexercise trials. Six male subjects ingested 100 g fructose or glucose at rest or after recovery from 45 min of treadmill exercise at 70% of maximal O2 consumption. Measurements of O2 consumption, respiratory exchange ratio, and plasma concentrations of glucose, insulin, glycerol, and lactate were measured for 3 h postingestion. Although glucose and fructose increased net energy expenditure by 44 and 51 kcal, respectively, over baseline during control trials, exercise increased the thermic effect of both carbohydrate challenges an additional 20-25 kcal (P less than 0.05). Glucose ingestion was associated with large (P less than 0.05) increases in plasma insulin concentration during control and exercise trials, in contrast to fructose ingestion. Because fructose, which is primarily metabolized by liver, and glucose elicited a similar postexercise potentiation of thermogenesis, the results indicate that the thermogenic phenomenon is not limited to skeletal muscle. These results also demonstrate that carbohydrate-induced postexercise thermogenesis is not related to an incremental increase in plasma insulin concentration.


Assuntos
Temperatura Corporal/efeitos dos fármacos , Exercício Físico/fisiologia , Frutose/farmacologia , Glucose/farmacologia , Adolescente , Adulto , Glicemia/metabolismo , Temperatura Corporal/fisiologia , Metabolismo Energético/efeitos dos fármacos , Metabolismo Energético/fisiologia , Frutose/sangue , Glicerol/sangue , Humanos , Insulina/sangue , Lactatos/sangue , Ácido Láctico , Masculino , Consumo de Oxigênio
13.
Am J Prev Med ; 15(4): 298-315, 1998 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-9838974

RESUMO

INTRODUCTION: Physical inactivity has been identified as an important public health concern for youth. School and community settings can be important infrastructures for promoting physical activity (PA). This paper reviews studies of physical activity in school and community settings among preschool through college-aged persons to determine characteristics and effects of interventions. Studies in progress are included. METHODS: Studies from 1980 to 1997 testing physical activity interventions in schools and community settings were identified by computerized search methods and reference lists of published reviews. Studies needed to have used a quantitative assessment of PA, used a comparison or control group, included participants who were preschool through college age, and be conducted in the United States or foreign school or community settings. Significance of effects was examined overall and for various types of interventions. RESULTS: Twenty-two school-based studies were reviewed, 14 completed and 8 in progress. Three studies were in countries other than the United States. The 8 studies in progress were all in the United States. Only 7 community studies were reviewed, all in the United States. Four studies were in progress. Several community studies involved a high percentage of African-American or Hispanic youth and their families. Studies showing the best results used randomized designs, valid and reliable measurements, and more extensive interventions. Some follow-up results showed PA was sustained after interventions ended. CONCLUSIONS: The collection of school and community studies is limited for several age groups with none below third grade and only three at college age. There are few community studies. The most is known about upper-elementary-age-students, including the first multicenter randomized trial to report significant results for increasing moderate to vigorous physical activity (MVPA) in physical education (PE) and increase vigorous PA outside of school. A number of older study designs were weak and assessments less than optimal, but studies in progress are stronger. Special attention is needed for girls, middle schools, and community settings for all youth. More objective assessments are needed for measuring PA outside of school and in younger children, since they cannot provide reliable self-report.


Assuntos
Exercício Físico , Promoção da Saúde , Educação Física e Treinamento , Aptidão Física , Adolescente , Adulto , Negro ou Afro-Americano , Fatores Etários , Criança , Estudos de Coortes , Currículo , Feminino , Hispânico ou Latino , Humanos , Atividades de Lazer , Masculino , Saúde Pública , Distribuição Aleatória , Pesquisa , Estudos de Amostragem , Instituições Acadêmicas , Estados Unidos
14.
Eur J Pharmacol ; 188(6): 399-401, 1990 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-2164941

RESUMO

In primary astrocyte cultures, picomolar concentrations of platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine, PAF) evoked the formation of inositol phosphates (InsP), including inositol trisphosphate. This effect was not observed with the biologically inert lyso-PAF, nor in cells pretreated with phorbol myristate acetate to downregulate receptors. PAF at concentrations greater than or equal to 10(-9) M did not elevate InsP, suggesting some form of uncoupling of the receptor from phospholipase C. The responsiveness of astrocytes to PAF is further evidence for the role of these cells in the central nervous system response to trauma.


Assuntos
Astrócitos/metabolismo , Fosfatidilinositóis/metabolismo , Fator de Ativação de Plaquetas/farmacologia , Animais , Animais Recém-Nascidos/metabolismo , Astrócitos/efeitos dos fármacos , Córtex Cerebral/citologia , Córtex Cerebral/efeitos dos fármacos , Córtex Cerebral/metabolismo , Hidrólise , Inosina Trifosfato/biossíntese , Fosfatos de Inositol/metabolismo , Ratos
15.
Neurosci Lett ; 109(1-2): 152-6, 1990 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-2156191

RESUMO

In response to ATP, astrocytes accumulate inositol phosphates and release arachidonic acid (AA) from phospholipid stores, events which may be linked through intracellular calcium mobilization and activation of phospholipase A2. When cells were exposed to ATP in the presence of serotonin there was a dose-dependent inhibition of AA release, an effect which was reversed by methysergide. However, the accumulation of inositol phosphates due to ATP was elevated in the presence of serotonin, and this effect was again reversed by methysergide. The mechanism by which serotonin inhibits ATP-induced arachidonate mobilization does not, therefore, involve the purinergic receptor or its coupling to inositol phospholipid pools. The hydrolysis of polyphosphoinositides by serotonin and subsequent generation of inositol trisphosphate may deplete a specific intracellular calcium store normally available to ATP, the mobilization of which stimulates phospholipase A2 and thus AA release.


Assuntos
Trifosfato de Adenosina/farmacologia , Ácidos Araquidônicos/metabolismo , Astrócitos/metabolismo , Córtex Cerebral/metabolismo , Fosfatidilinositóis/metabolismo , Serotonina/farmacologia , Animais , Ácido Araquidônico , Astrócitos/citologia , Astrócitos/efeitos dos fármacos , Células Cultivadas , Córtex Cerebral/citologia , Ratos
16.
Neurosci Lett ; 138(2): 275-8, 1992 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-1608538

RESUMO

The gene for the epidermal growth factor (EGF) receptor is amplified in a variety of neoplastic tissues, including malignant gliomas. To reveal whether increased sensitivity to EGF has significance for the supply of metabolic substrate to tumor cells, the rate of glucose transport was determined in cells exposed to EGF for up to six hours. In the epidermoid carcinoma line A431, and in primary cultures from 7/12 human glioma biopsies, EGF (10 ng/ml) induced an increase (two-fold) in glucose transport. This effect was transient and independent of protein synthesis.


Assuntos
Astrocitoma/metabolismo , Fator de Crescimento Epidérmico/farmacologia , Glucose/farmacocinética , Transporte Biológico/efeitos dos fármacos , Cicloeximida/farmacologia , Desoxiglucose/farmacocinética , Humanos , Fatores de Tempo , Células Tumorais Cultivadas , Regulação para Cima
17.
Med Sci Sports Exerc ; 32(9 Suppl): S481-8, 2000 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-10993418

RESUMO

PURPOSE: The Digi-Walker step counter is a promising and cost-effective tool to measure physical activity under free-living conditions. Two specific studies were conducted to evaluate the number of steps required to meet current physical activity guidelines. METHODS: Thirty-one adults (17 men, 14 women) served as participants. In study 1, we determined the number of steps to complete a mile under two different conditions and three paces. In study 2, we conducted a field trial to examine the relationship between daily step counts and other indices of physical activity. Participants in this study wore a Digi-Walker for 2 consecutive weeks and completed the 7-d physical activity recall (PAR) after each week. RESULTS: In study 1, there were no differences in step counts by site, but steps were inversely related to pace, with values ranging from 1330 to 1996. Individual step counts at a specific pace were negatively correlated with height, weight, leg length, and stride length and were positively correlated with body fatness. In study 2, participants had average daily step counts of 11,603 when structured vigorous activity was included and 8265 when only light and moderate activity were measured. Modest correlations were found between step counts and estimated energy expenditure. Similar correlations were observed when step counts were related to minutes of activity per day and minutes of sitting per day. CONCLUSIONS: Pedometers provide a useful indicator of daily step counts but variability in activity patterns make it difficult to establish step count guidelines that correspond with other public health guidelines.


Assuntos
Atividades Cotidianas , Aptidão Física , Caminhada , Adulto , Análise Custo-Benefício , Teste de Esforço/instrumentação , Feminino , Marcha , Humanos , Masculino , Sensibilidade e Especificidade
18.
Med Sci Sports Exerc ; 32(9 Suppl): S489-97, 2000 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-10993419

RESUMO

PURPOSE: Accelerometry-based activity monitors offer promise for the assessment of free-living physical activity. They provide an objective record of frequency, intensity, and duration of physical activity with minimal burden on participants. The purpose of this study was to evaluate the absolute and relative validity of three contemporary activity monitors (Computer Science and Applications, Inc. [CSA], Tritrac, and Biotrainer) under both laboratory and field conditions. METHODS: Fifty-two participants completed two 30-min choreographed routines designed to simulate a variety of lifestyle physical activities. Three different treadmill paces were completed in both routines to evaluate reliability and validity under laboratory conditions. Six different lifestyle activities were also examined to evaluate the validity of the monitors under field conditions. During each routine, the activity levels of participants were monitored with the three activity monitors as well as by indirect calorimetry systems. RESULTS: The correlations between the monitors and measured VO2 were higher for treadmill activity (mean r = 0.86) compared with lifestyle activity (mean r = 0.55). Correlations among the different monitors were high for both treadmill (r = 0.86) and lifestyle activities (r = 0.70), suggesting that the monitors provide similar information under both conditions. Under laboratory conditions, the CSA yielded accurate predictions of energy expenditure (EE), whereas the Tritrac and Biotrainer tended to overestimate the EE (101-136% of measured value). The Tritrac, however, was found to have less error in individual estimates of EE. Under field conditions, all of the monitors underestimated EE (range: 42-67% of measured value). CONCLUSION: The observed differences among the monitors were attributed primarily to differences in the accuracy of the calibration equations rather than to the monitors themselves. Further research is needed to better understand how to use these devices for field-based assessments of physical activity.


Assuntos
Atividades Cotidianas , Aptidão Física , Adulto , Teste de Esforço/instrumentação , Teste de Esforço/métodos , Feminino , Humanos , Masculino , Movimento (Física) , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
19.
Ann Behav Med ; 29 Suppl: 35-45, 2005 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15921488

RESUMO

The purpose of this study was to identify the population prevalence across the stages of change (SoC) for regular physical activity and to establish the prevalence of people at risk. With support from the National Institutes of Health, the American Heart Association, and the Robert Wood Johnson Foundation, nine Behavior Change Consortium studies with a common physical activity SoC measure agreed to collaborate and share data. The distribution pattern identified in these predominantly reactively recruited studies was Precontemplation (PC) = 5% (+/- 10), Contemplation (C) = 10% (+/- 10), Preparation (P) = 40% (+/- 10), Action = 10% (+/- 10), and Maintenance = 35% (+/- 10). With reactively recruited studies, it can be anticipated that there will be a higher percentage of the sample that is ready to change and a greater percentage of currently active people compared to random representative samples. The at-risk stage distribution (i.e., those not at criteria or PC, C, and P) was approximately 10% PC, 20% C, and 70% P in specific samples and approximately 20% PC, 10% C, and 70% P in the clinical samples. Knowing SoC heuristics can inform public health practitioners and policymakers about the population's motivation for physical activity, help track changes over time, and assist in the allocation of resources.


Assuntos
Pesquisa Comportamental/métodos , Pesquisa Comportamental/normas , Comportamentos Relacionados com a Saúde , Promoção da Saúde , Atividade Motora , Humanos
20.
Res Q Exerc Sport ; 66(3): 202-9, 1995 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-7481081

RESUMO

The purpose of this study was to evaluate the validity of the Tritrac-R3D Activity Monitor, a new instrument designed to improve assessments of physical activity. Comparisons were made with a heart rate monitor and with a Caltrac Activity Monitor. Thirty-five children (ages 9-11 years) were monitored on 3 different school days with all 3 instruments. The Tritrac was moderately correlated with the heart rate monitor (r = .58) and highly correlated with the Caltrac monitor (r = .88). By taking advantage of the minute-by-minute timing capability of the Tritrac and the heart rate monitors, it was discovered that the correlations between these instruments were highest during free play situations (lunch/recess, recess, after school) and were lower when activity was more limited (class time) or structured (physical education). The ability of the Tritrac to assess activity on a minute-by-minute basis may greatly enhance its overall utility.


Assuntos
Exercício Físico , Criança , Metabolismo Energético , Exercício Físico/fisiologia , Frequência Cardíaca , Humanos , Masculino , Monitorização Fisiológica/instrumentação , Reprodutibilidade dos Testes
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