Extracellular Vesicle Refractive Index Derivation Utilizing Orthogonal Characterization.

The analysis of small particles, including extracellular vesicles and viruses, is contingent on their ability to scatter sufficient light to be detected. These detection methods include flow cytometry, nanoparticle tracking analysis, and single particle reflective image sensing. To standardize measurements and enable orthogonal comparisons between platforms, a quantifiable limit of detection is required. The main parameters that dictate the amount of light scattered by particles include size, morphology, and refractive index. To date, there has been a lack of accessible techniques for measuring the refractive index of nanoparticles at a single-particle level. Here, we demonstrate two methods of deriving a small particle refractive index using orthogonal measurements with commercially available platforms. These methods can be applied at either a single-particle or population level, enabling the integration of diameter and scattering cross section values to derive the refractive index using Mie theory.

Bioanalytical and non-targeted mass spectrometric screening for contaminants of emerging concern in Southern California bight sediments.

Assessing the impact of chemical contaminants on aquatic ecosystem health remains challenging due to complex exposure scenarios and the myriad of impact metrics to consider. To expand the breadth of compounds monitored and evaluate the potential hazard of environmental mixtures, cell-based bioassays (estrogen receptor alpha (ERα) and aryl hydrocarbon receptor (AhR)) and non-targeted chemical analyses with high resolution mass spectrometry (NTA-HRMS) were used to assess the quality of ∼70 marine sediment samples collected from 5 distinct coastal and offshore habitats of the Southern California Bight. AhR responses (<0.12-4.5 ng TCDD/g dry weight) were more frequently detectable and more variable than for ERα (<0.1-0.5 ng E2/g dry weight). The range of AhR and ERα responses increased by habitat as follows: Channel Islands < Mid-shelf < Marinas < Ports < Estuaries. The narrow range and magnitude of ERα screening response suggested limited potential for estrogenic impacts across sediments from all 5 habitats. The AhR response was positively correlated with total PAH and PCB concentrations and corresponded with a chemical score index representing the severity of metal and organic contamination. NTA-HRMS fingerprints generated in positive electrospray ionization mode were clearly distinguishable among coastal vs. offshore samples, with the greatest chemical complexity (n = 982 features detected) observed in estuarine sediment from a highly urbanized watershed (Los Angeles River). The concordance and complementary nature of bioscreening and NTA-HRMS results indicates their utility as holistic proxies for sediment quality, and when analyzed in conjunction with routine targeted chemical monitoring, show promise in identifying unexpected contaminants and novel toxicants.

Application of an Effects-Based Monitoring Strategy to Assess the Impact of Contaminants on Fish Health in an Urbanized Watershed.

Effects-based monitoring frameworks that combine the use of analytical chemistry with in vitro cell bioassays, as well as in vivo whole organism tests offer an integrative approach to broadly screen for chemical contaminants and link their presence with adverse effects on aquatic organisms. California (USA) is currently evaluating the use of such a framework to assess the impact of contaminants of emerging concern (CECs) on biota in urbanized rivers and other waterbodies. In the present study, the occurrence and effects of contaminants found in the Los Angeles River (Los Angeles County, CA, USA) were examined using analytical chemistry and in vitro and in vivo bioassays. Male fathead minnows were deployed in field-based exposure units and exposed to river water for 21 d. The 2 field sites (above Bull Creek [BLC] and below Glendale Water Reclamation Plant [GWR]) were selected based on their unique characteristics and different contaminant discharge sources. In addition, 2 control units (filtered city water and estrone-spiked water) were added to the experimental design. Chemical analyses revealed differences in abundance of CECs between the 2 field sites and the controls, with GWR having the highest number and concentrations of CECs and metals. Cell bioassays screening for estrogenic, glucocorticoid, progestin, and dioxin-like activities were near or below detection limits in all river water samples, indicating a low potential for endocrine-related toxicity and tissue damage. Cell bioassay results were corroborated by the in vivo analyses. Field-exposed fish exhibited no changes in plasma hormones (e.g., estradiol), vitellogenin, or gonad maturation, but gene biomarkers of chemical exposure (cytochrome p450 1A and metallothionein) were significantly elevated, confirming exposure of the fish to complex chemical mixtures. The results demonstrate the value of a tiered monitoring approach to assess the sublethal effects of chemical mixtures on aquatic life. Environ Toxicol Chem 2021;40:402-412. © 2020 SETAC.

Synthesis of ecotoxicological studies on cyanotoxins in freshwater habitats - Evaluating the basis for developing thresholds protective of aquatic life in the United States.

In recent decades, cyanobacteria harmful algal blooms (cyanoHABs) have increased in magnitude, frequency, and duration in freshwater ecosystems. CyanoHABs can impact water quality by the production of potent toxins known as cyanotoxins. Environmental exposure to cyanotoxins has been associated with severe illnesses in humans, domestic animals, and wildlife. However, the effects of sustained exposure to cyanotoxins on aquatic life are poorly understood. In this study, over 150 peer-reviewed articles were critically evaluated to better understand the ecotoxicity of 5 cyanotoxin classes (microcystins, cylindrospermopsin, anatoxin-a, saxitoxins, nodularin) on fish, amphibians, aquatic invertebrates, and birds exclusively feeding in freshwater habitats. The systemic review demonstrated that microcystins, and more specifically microcystin-LR, were the most studied cyanotoxins. Ecotoxicological investigations were typically conducted using a fish or aquatic invertebrate model, with mortality, bioaccumulation, and biochemical responses as the most frequently measured endpoints. After excluding the studies that did not meet our acceptability criteria, remaining studies were examined to identify the no-observed and lowest observed effect concentrations (NOEC and LOEC) for microcystins; the limited amount of data for other cyanotoxins did not allow for analysis. The published ecotoxicity data suggests that the U.S. EPA recreational water quality criteria for microcystin (8 µg/L) may be protective of acute toxicity in aquatic organisms but does not appear to protect against chronic toxicity. Individual U.S. states have developed more stringent recreational health-based thresholds, such as 0.8 µg/L in California. Comparisons of this threshold to the chronic NOEC and LOEC data indicate that more restrictive microcystins thresholds may be required to be protective of aquatic life. Additional research is needed to evaluate the sublethal effects of a wider array of microcystin congeners and other cyanotoxins on organisms relevant to U.S. watersheds to better support nationwide thresholds protective of aquatic life.