[Research on association between atherosclerotic brain infarction and human leukocyte antigen DR gene].

OBJECTIVE: To evaluate the relationship between atherosclerotic brain infarction (ABI) and human leukocyte antigen DR (HLA-DR) gene at molecular level. METHODS: By using polymerase chain reaction sequence specific probe (PCR-SSP), gene special position of the HLA-II DR alleles were determined in 31 patients and 30 healthy individuals as controls, all of them were inhabitants of Northeastern China. RESULTS: The HLA-DR B1*0301 gene frequency in the group of ABI was obviously higher than that of control group (RR=5.6842, P<0.05). CONCLUSION: The HLA-DR B1*0301 gene may be the susceptible gene of ABI in Northeastern China, or it may associate with other genes to produce the disease.

[Relationship between polymorphisms of cystathionine beta-synthase gene and stroke].

OBJECTIVE: To determine whether the T27796C mutation in cystathionine beta-synthase (CBS) gene is associated with stroke in Chinese. METHODS: The T27796C mutation in CBS gene of 59 cases with stroke and 65 health controls were detected by polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP). RESULTS: As this investigation shown, the frequencies of two alleles of T27796C mutation were followed: C was 56.8%, T was 43.2% in cases and C was 51.5%, T was 48.5% in controls. T27796C mutation had no obvious relativity to the stroke (P>0.05). There was no significant difference in frequency of alleles of this mutation between cases and controls (CC: 35.6% vs. 24.6%; CT: 42.4% vs. 53.8%; TT: 22.0% vs. 21.5%; all P>0.05). There was not obvious difference in frequency of alleles of this mutation between hemorrhagic patients and ischemic stroke patients (all P>0.05). CONCLUSION: T27796C mutation in CBS is not obviously correlated with stroke and this mutation is not associated with categories of stroke.