Combined effect of salt and drought on boron toxicity in Puccinellia tenuiflora.

Boron toxicity is a worldwide problem, usually accompanied by salt (NaCl) and drought. The combined stresses may induce complex toxicity to the plant. The aim of the present study was to investigate how the combined stresses of salt and drought affect B toxicity in plants. Puccinellia tenuiflora seedlings were planted in vermiculite. A three (B) × three (salt) × three (drought) factorial experiment (for a total of 27 treatments) was conducted. After a 30-day cultivation, plants were harvested to determine dry weight and the concentrations of B, Na+, K+, Ca2+, and Mg2+. Plant growth was inhibited by B toxicity, which was alleviated by salt and drought. B stress enhanced B uptake and transport of the plant, which was inhibited by salt and drought. B stress had a little effect on K+ and Na+ concentration and caused Ca2+ and Mg2+ accumulation in the plant. Salt addition increased Na+ concentration and inhibited Ca2+ and Mg2+ accumulation. Drought addition inhibited Na+ accumulation and enhanced Ca2+ and Mg2+ accumulation. The combined stresses of salt and drought had a greater alleviation on the inhibition of dry weight caused by B than individual salt and drought. Besides, the combined stresses of salt and drought also enhanced B uptake and inhibited B transport. The results indicate that salt, drought, and the combined stresses of salt and drought all can alleviate B toxicity in P. tenuiflora, the main mechanism of which is the restriction of B and Na+ uptake caused by salt and drought. The combined stresses of salt and drought have a greater effect on B toxicity than individual salt and drought.

Myriophyllum elatinoides: A potential candidate for the phytoremediation of water with low level boron contamination.

Phytoremediation is considered to be a cost-effective strategy for removing boron (B) from polluted water. In this study, Myriophyllum elatinoides, a widespread submerged or floating macrophyte, was found to survive in 40 mg B/L. Time-dependent kinetics show that the shoot exhibits a much longer period of B uptake and a much higher maximal tissue B concentration than the root. High values of the bioconcentration factor (BCF) and translocation factor (TF) indicate that M. elatinoides is a potential hyperaccumulator of B. Transmission electron micrographs show that excess B damages the cells of M. elatinoides, and the major target organelles are the chloroplast (leaf), mitochondria (stem and root), and nucleolus (root). Energy dispersive spectroscopy (EDS) shows that B is mainly deposited in the cytoplasm and on the surface of the chloroplast of the leaf cell. In the stem and root cells, B is mainly deposited on the mitochondrial membrane and in the vacuoles, respectively. This study indicates that the mechanisms of B toxicity, tolerance, and accumulation in M. elatinoides are involved in the cellular localization of B. Future work should focus on the evaluation of the physiological and genetic mechanisms involved in B tolerance and accumulation in M. elatinoides under different conditions.