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1.
Anal Biochem ; 608: 113854, 2020 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-32745543

RESUMO

With the rapid development of human's exploitation of nature and animal husbandry, zoonoses have become a major public health problem worldwide. It is necessary to establish a rapid, specific and sensitive detection method to screen several zoonotic pathogenic bacteria simultaneously. In this study, phage display technology was used to screen specific peptide of three common zoonotic pathogens, E. coli O157:H7, L. monocytogenes and B. melitensis 16 M. Then, three peptide were obtained, named E2, L4 and B4, which can identify the three pathogens respectively. Three peptide modified with biotin were synthesized and were coupled to streptavidin magnetic beads (MBs) to form peptide-MBs, which enriched the above three pathogens from the samples. Three quantum dot (QD) probes were constructed by coupling three polyclonal antibodies to different fluorescent QD surfaces (QD540, QD580 and QD630). The simultaneous detection method based on peptide-MBs and QDs multicolor fluorescent labeling was established and could detect E. coli O157:H7, L. monocytogenes and B. melitensis 16 M simultaneously. The detection method took about 100 min with the detection limits of 103, 102 and 102 CFU/mL, respectively. The detection method could be also well utilized in real samples.


Assuntos
Bactérias/patogenicidade , Técnicas de Química Analítica/métodos , Colorimetria/métodos , Separação Imunomagnética/métodos , Biblioteca de Peptídeos , Peptídeos/química , Pontos Quânticos , Animais , Biotina/química , Brassica/microbiologia , Brucella melitensis/química , Contagem de Colônia Microbiana , Escherichia coli O157/química , Fluorimunoensaio/métodos , Contaminação de Alimentos/análise , Limite de Detecção , Listeria monocytogenes/química , Espectrometria de Fluorescência/métodos , Estreptavidina/química
2.
Int J Mol Sci ; 21(19)2020 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-32992627

RESUMO

InP QDs have shown a great potential as cadmium-free QDs alternatives in biomedical applications. It is essential to understand the biological fate and toxicity of InP QDs. In this study, we investigated the in vivo renal toxicity of InP/ZnS QDs terminated with different functional groups-hydroxyl (hQDs), amino (aQDs) and carboxyl (cQDs). After a single intravenous injection into BALB/c mice, blood biochemistry, QDs distribution, histopathology, inflammatory response, oxidative stress and apoptosis genes were evaluated at different predetermined times. The results showed fluorescent signals from QDs could be detected in kidneys during the observation period. No obvious changes were observed in histopathological detection or biochemistry parameters. Inflammatory response and oxidative stress were found in the renal tissues of mice exposed to the three kinds of QDs. A significant increase of KIM-1 expression was observed in hQDs and aQDs groups, suggesting hQDs and aQDs could cause renal involvement. Apoptosis-related genes (Bax, Caspase 3, 7 and 9) were up-regulated in hQDs and aQDs groups. The above results suggested InP/ZnS QDs with different surface chemical properties would cause different biological behaviors and molecular actions in vivo. The surface chemical properties of QDs should be fully considered in the design of InP/ZnS QDs for biomedical applications.


Assuntos
Índio/química , Índio/toxicidade , Rim/efeitos dos fármacos , Fosfinas/química , Fosfinas/toxicidade , Pontos Quânticos/química , Pontos Quânticos/toxicidade , Animais , Apoptose/efeitos dos fármacos , Apoptose/genética , Dióxido de Carbono/química , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Radical Hidroxila/química , Índio/administração & dosagem , Índio/farmacocinética , Inflamação/induzido quimicamente , Injeções Intravenosas , Rim/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Estresse Oxidativo/efeitos dos fármacos , Fosfinas/administração & dosagem , Fosfinas/farmacocinética , Pontos Quânticos/administração & dosagem , Sulfetos/administração & dosagem , Sulfetos/química , Sulfetos/farmacocinética , Sulfetos/toxicidade , Propriedades de Superfície , Distribuição Tecidual , Compostos de Zinco/administração & dosagem , Compostos de Zinco/química , Compostos de Zinco/farmacocinética , Compostos de Zinco/toxicidade
3.
Sci Rep ; 14(1): 3075, 2024 02 06.
Artigo em Inglês | MEDLINE | ID: mdl-38321064

RESUMO

Non-alcoholic fatty liver disease (NAFLD) is one of the most common liver diseases worldwide and there is a huge unmet need to find safer and more effective drugs. Vitamin K has been found to regulate lipid metabolism in the liver. However, the effects of vitamin K2 on NAFLD is unclear. This study aims to evaluate the preventive and therapeutic effects of vitamin K2 in the process of fatty liver formation and to explore molecular mechanisms the associated with lipid metabolism. A non-alcoholic fatty liver model was established by high-fat diet administration for three months. Vitamin K2 significantly reduced the body weight, abdominal circumference and body fat percentage of NAFLD mice. Vitamin K2 also showed histological benefits in reducing hepatic steatosis. NAFLD mice induced by high-fat diet showed increased HMGR while vitamin K2 intervention could reverse the pathological lterations. Adiponectin (APN) is an endogenous bioactive polypeptide or protein secreted by adipocytes. We detected APN, SOD, AlaDH and other indicators that may affect the state of high-fat diet mice, but the experimental results showed that the above indicators did not change significantly. It is worth noting that the effect of vitamin K2 supplementation on the lipid-lowering effect of uc OC in vivo needs to be further explored. This study first reported the protective effect of vitamin K2 on high-fat diet-induced NAFLD in mice. The protective effect of vitamin K2 may be related to the improvement of lipid metabolism disorder in NAFLD.


Assuntos
Hepatopatia Gordurosa não Alcoólica , Camundongos , Animais , Hepatopatia Gordurosa não Alcoólica/patologia , Vitamina K 2/metabolismo , Dieta Hiperlipídica , Fígado/metabolismo , Metabolismo dos Lipídeos , Adiponectina/metabolismo , Camundongos Endogâmicos C57BL
4.
Heliyon ; 10(6): e26940, 2024 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-38509943

RESUMO

Diabetic wound infections caused by the multiplication of infectious pathogens and their antibiotic resistance. Wound infection evident by bacterial colonization and other factors, such as the virulence and host immune factors. In this context, we need discover appropriate treatment and effective antibiotics for wound infection control. Considering this, we synthesized catechin-loaded polyvinyl alcohol/Chitosan (PVA/CS) based nanofiber for multifunctional wound healing. The physicochemical and biological properties of fabricated nanofiber, were systematically evaluated by various spectroscopy and microscopy techniques. The CA@PVA/CS nanofiber exhibited a high level of antibacterial and antioxidant effects. The nanofibers showed effective control in gram-positive and negative wound infectious bacterial multiplication at the lowest concentration. Based on the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability study CA@PVA/CS nanofiber shows excellent biocompatibility against L929 cells. In wound, scratch assay results revealed that the CA@PVA/CS treated group shows enhanced cell migration and cell proliferation within 48 h. The synthesis of antioxidant, antibacterial, and biocompatible nanofiber exposes their potential for effective wound healing. Current research hypothesized catechin loaded PVA/CS nanofiber could be a multifunctional and low-cost material for diabetic wound care application. Fabricated nanofiber would be improved skin tissue regeneration and public health hygiene.

5.
Front Comput Neurosci ; 17: 1284966, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37927547

RESUMO

This paper investigates the containment control of multiple unmanned surface vessels with nonlinear dynamics. To solve the leader-follower synchronization problem in a containment control system, a hierarchical control framework with a topology reconfiguration mechanism is proposed, and the process of containment control is converted into the tracking of a reference signal for each vessel on its respective target heading by means of the light-of-sight (LOS) guidance. In a control system, the neural networks (NNs) are adopted to consider the uncertainty. In the follower layer, a connectivity controller with a topology reconfiguration mechanism is embedded, to change the converging positions of followers so as to avoid collision within the system, and to maintain the system connectivity when the communication equality is poor. The effectiveness of the hierarchical control framework proposed in this paper is valid by simulation.

6.
Int J Mol Med ; 47(3)2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33448308

RESUMO

Vitamin K­dependent proteins (VKDPs) are a group of proteins that need vitamin K to conduct carboxylation. Thus far, scholars have identified a total of 17 VKDPs in the human body. In this review, we summarize three important emerging VKDPs: Growth arrest­specific protein 6 (Gas 6), Gla­rich protein (GRP) and periostin in terms of their functions in physiological and pathological conditions. As examples, carboxylated Gas 6 and GRP effectively protect blood vessels from calcification, Gas 6 protects from acute kidney injury and is involved in chronic kidney disease, GRP contributes to bone homeostasis and delays the progression of osteoarthritis, and periostin is involved in all phases of fracture healing and assists myocardial regeneration in the early stages of myocardial infarction. However, periostin participates in the progression of cardiac fibrosis, idiopathic pulmonary fibrosis and airway remodeling of asthma. In addition, we discuss the relationship between vitamin K, VKDPs and cancer, and particularly the carboxylation state of VKDPs in cancer.


Assuntos
Moléculas de Adesão Celular/metabolismo , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Infarto do Miocárdio/metabolismo , Osteoartrite/metabolismo , Insuficiência Renal Crônica/metabolismo , Vitamina K/metabolismo , Animais , Humanos , Infarto do Miocárdio/patologia , Osteoartrite/patologia , Insuficiência Renal Crônica/patologia
7.
Front Cardiovasc Med ; 8: 753313, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34859069

RESUMO

With the gradual prolongation of the overall survival of cancer patients, the cardiovascular toxicity associated with oncology drug therapy and radiotherapy has attracted increasing attention. At present, the main methods to identify early cancer treatment-related cardiac dysfunction (CTRCD) include imaging examination and blood biomarkers. In this review, we will summarize the research progress of subclinical CTRCD-related blood biomarkers in detail. At present, common tumor therapies that cause CTRCD include: (1) Chemotherapy-The CTRCD induced by chemotherapy drugs represented by anthracycline showed a dose-dependent characteristic and most of the myocardial damage is irreversible. (2) Targeted therapy-Cardiovascular injury caused by molecular-targeted therapy drugs such as trastuzumab can be partially or completely alleviated via timely intervention. (3) Immunotherapy-Patients developed severe left ventricular dysfunction who received immune checkpoint inhibitors have been reported. (4) Radiotherapy-CTRCD induced by radiotherapy has been shown to be significantly associated with cardiac radiation dose and radiation volume. Numerous reports have shown that elevated troponin and B-type natriuretic peptide after cancer treatment are significantly associated with heart failure and asymptomatic left ventricular dysfunction. In recent years, a few emerging subclinical CTRCD potential biomarkers have attracted attention. C-reactive protein and ST2 have been shown to be associated with CTRCD after chemotherapy and radiation. Galectin-3, myeloperoxidas, placental growth factor, growth differentiation factor 15 and microRNAs have potential value in predicting CTRCD. In this review, we will summarize CTRCD caused by various tumor therapies from the perspective of cardio-oncology, and focus on the latest research progress of subclinical CTRCD biomarkers.

8.
Technol Cancer Res Treat ; 19: 1533033820965574, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33084530

RESUMO

BACKGROUND: Tissue differentiation-inducing non-protein coding RNA (TINCR) has been shown to play a crucial role in pathogenesis of various types of human cancer including breast cancer (BC). The purpose of this study was to determine the potential prognostic value of serum lncRNA TINCR in BC. METHODS: Quantitative reverse transcription PCR (qRT-PCR) was performed to detect serum lncRNA TINCR levels in 72 triple-negative BC (TNBC) patients, 105 non-TNBC patients, 60 benign breast disease patients and 86 healthy subjects. RESULTS: The results showed that serum lncRNA TINCR level was significantly increased in BC, especially in TNBC. High circulating lncRNA TINCR was significantly correlated with worse clinicopathological features and clinical outcome of TNBC. Multivariate analysis revealed that serum lncRNA TINCR was an independent prognostic factor for overall survival of TNBC. However, little association was found between serum lncRNA TINCR and the prognosis of non-TNBC. CONCLUSIONS: Taken together, our findings demonstrate that serum lncRNA TINCR might be a useful novel and non-invasive biomarker for the prognosis prediction of TNBC.


Assuntos
Biomarcadores Tumorais/sangue , Prognóstico , RNA Longo não Codificante/sangue , Neoplasias de Mama Triplo Negativas/sangue , Linhagem Celular Tumoral , Intervalo Livre de Doença , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Pessoa de Meia-Idade , Neoplasias de Mama Triplo Negativas/diagnóstico , Neoplasias de Mama Triplo Negativas/patologia
9.
Front Pharmacol ; 10: 1194, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31680969

RESUMO

Recently, RNA interfering (RNAi) has become a promising approach for cancer therapy. However, the application of RNAi for clinics is still hindered due to the lack of safe and efficient carriers. In this study, a pH-responsive micelle based on polycaprolactone-block-poly 2-(dimethylamino)ethyl methacrylate (PCL-PDEM) cationic copolymer was developed to carry short interfering RNA (siRNA) for silencing interleukin 8 (IL-8) gene in hepatoma cancer cells. The transfection efficiency of the PCL-PDEM-siRNA/quantum dots (QDs) nanoplex has reached about 70%, and the expression level of IL-8 decreased about 63%. Furthermore, the codelivery of QDs and siRNA has been realized, which is beneficial to visualize the process of siRNA delivery. No considerable cytotoxicity from the nanoparticles has been observed, indicating that our responsive cationic micelle is potential in clinical trial for hepatoma cancer therapy.

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