MAMI: a comprehensive database of mother-infant microbiome and probiotic resources.

Extensive evidence has demonstrated that the human microbiome and probiotics confer great impacts on human health, particularly during critical developmental stages such as pregnancy and infancy when microbial communities undergo remarkable changes and maturation. However, a major challenge in understanding the microbial community structure and interactions between mothers and infants lies in the current lack of comprehensive microbiome databases specifically focused on maternal and infant health. To address this gap, we have developed an extensive database called MAMI (Microbiome Atlas of Mothers and Infants) that archives data on the maternal and neonatal microbiome, as well as abundant resources on edible probiotic strains. By leveraging this resource, we can gain profound insights into the dynamics of microbial communities, contributing to lifelong wellness for both mothers and infants through precise modulation of the developing microbiota. The functionalities incorporated into MAMI provide a unique perspective on the study of the mother-infant microbiome, which not only advance microbiome-based scientific research but also enhance clinical practice. MAMI is publicly available at https://bioinfo.biols.ac.cn/mami/.

Microbial transmission, colonisation and succession: from pregnancy to infancy.

The microbiome has been proven to be associated with many diseases and has been used as a biomarker and target in disease prevention and intervention. Currently, the vital role of the microbiome in pregnant women and newborns is increasingly emphasised. In this review, we discuss the interplay of the microbiome and the corresponding immune mechanism between mothers and their offspring during the perinatal period. We aim to present a comprehensive picture of microbial transmission and potential immune imprinting before and after delivery. In addition, we discuss the possibility of in utero microbial colonisation during pregnancy, which has been highly debated in recent studies, and highlight the importance of the microbiome in infant development during the first 3 years of life. This holistic view of the role of the microbial interplay between mothers and infants will refine our current understanding of pregnancy complications as well as diseases in early life and will greatly facilitate the microbiome-based prenatal diagnosis and treatment of mother-infant-related diseases.

TGase-induced Cd tolerance by boosting polyamine, nitric oxide, cell wall composition and phytochelatin synthesis in tomato.

In highly intensive greenhouse vegetable production, soil acidification was caused by excessive fertilization, increasing cadmium (Cd) concentrations in the vegetables, which bears environmental hazards and is a negative influence on vegetables and humans. Transglutaminases (TGases), a central mediator for certain physiological effects of polyamines (PAs) in the plant kingdom, play important roles in plant development and stress response. Despite increased research on the crucial role of TGase in protecting against environmental stresses, relatively little is known about the mechanisms of Cd tolerance. In this study, we found, TGase activity and transcript level, which was upregulated by Cd, and TGase-induced Cd tolerance related to endogenous bound PAs increase and formation of nitric oxide (NO). Plant growth of tgase mutants was hypersensitive to Cd, chemical complementation by putrescine, sodium nitroprusside (SNP, nitric oxide donor) or gain of function TGase experiments restore Cd tolerance. α-diflouromethylornithine (DFMO, a selective ODC inhibitor) and 2-4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO, NO scavenger), were respectively found declined drastically endogenous bound PA and NO content in TGase overexpression plants. Likewise, we reported that TGase interacted with polyamine uptake protein 3 (Put3), and the silencing of Put3 largely reduced TGase-induced Cd tolerance and bound PAs formation. This salvage strategy depends on TGase-regulated synthesis of bound PAs and NO that is able to positively increase the concentration of thiol and phytochelatins, elevate Cd in the cell wall, as well as induce the levels of expression Cd uptake and transport genes. Collectively, these findings indicate that TGase-mediated enhanced levels of bound PA and NO acts as a vital mechanism to protect the plant from Cd-caused toxicity.

Recent Implementations of Hydrogel-Based Microbial Electrochemical Technologies (METs) in Sensing Applications.

Hydrogel materials have been used extensively in microbial electrochemical technology (MET) and sensor development due to their high biocompatibility and low toxicity. With an increasing demand for sensors across different sectors, it is crucial to understand the current state within the sectors of hydrogel METs and sensors. Surprisingly, a systematic review examining the application of hydrogel-based METs to sensor technologies has not yet been conducted. This review aimed to identify the current research progress surrounding the incorporation of hydrogels within METs and sensors development, with a specific focus on microbial fuel cells (MFCs) and microbial electrolysis cells (MECs). The manufacturing process/cost, operational performance, analysis accuracy and stability of typical hydrogel materials in METs and sensors were summarised and analysed. The current challenges facing the technology as well as potential direction for future research were also discussed. This review will substantially promote the understanding of hydrogel materials used in METs and benefit the development of electrochemical biosensors using hydrogel-based METs.

Application of 3D Printing Technology in Sensor Development for Water Quality Monitoring.

The development of sensors for water quality monitoring is crucial to protect water quality, aquatic biota and human health. Traditional sensor manufacturing methods have significant drawbacks, such as low fabrication freedom, limited material choice and expensive manufacturing cost. As a possible alternative method, 3D printing technologies are increasingly popular in sensor development due to their high versatility, fast fabrication/modification, powerful processing of different materials and ease of incorporation with other sensor systems. Surprisingly, a systematic review examining the application of 3D printing technology in water monitoring sensors has not yet been conducted. Here, we summarized the development history, market share and advantages/disadvantages of typical 3D printing techniques. Specifically focused on the 3D-printed sensor for water quality monitoring, we then reviewed the applications of 3D printing in the development of sensors' supporting platform, cell, sensing electrode as well as all-3D-printed sensors. The fabrication materials and processing, and the sensor's performances regarding detected parameters, response time and detection limit/sensitivity, were also compared and analyzed. Finally, the current drawbacks of 3D-printed water sensors and potential directions for future study were discussed. This review will substantially promote the understanding of 3D printing technology used in water sensor development and benefit the protection of water resources.

The efficiency of quick response code versus telephone contact for post-discharge follow-up after ophthalmic day surgery: a randomized controlled trial.

PURPOSE: To assess and compare the efficiency of quick response (QR) code versus telephone contact for post-discharge follow-up in patients receiving low-risk ophthalmic day surgery. METHODS: One hundred and sixty patients undergoing strabismus day-care surgery with general anesthesia were randomly allocated to either the intervention group using QR code (QR group) or the control group receiving telephone call (TEL group) for post-discharge follow-up. The primary outcome was the follow-up overall attendance rate on the second postoperative day. Secondary outcomes included attendance rate at the first scheduled follow-up, number of text message reminders, elapsed time and estimated cost for follow-up, omission rate of follow-up responses, and patient satisfaction. RESULTS: The overall attendance rate of follow-up was significantly higher in the QR group than that in the TEL group (97.5% vs. 87.5%, p = 0.016). As compared with the TEL group, the QR group significantly reduced the number of text message reminders with higher attendances at the first scheduled follow-up (p < 0.001, p = 0.001). Besides, the TEL group cost a median time of 258 s and a median cost of RMB 5.8 yuan to complete a follow-up consultant, but was associated with a significantly high omission rate of follow-up responses comparing to the QR group (p = 0.002). Patient satisfaction was comparable between two groups. CONCLUSION: QR code follow-up can be more efficient than traditional telephone contact in assessing the post-discharge recovery after strabismus day surgery, which provides a safe and intuitively alternative follow-up pathway for identifying issues that may necessitate further clinical care for more low-risk ophthalmic day surgeries.

Unfavourable intrauterine environment contributes to abnormal gut microbiome and metabolome in twins.

OBJECTIVE: Fetal growth restriction (FGR) is a devastating pregnancy complication that increases the risk of perinatal mortality and morbidity. This study aims to determine the combined and relative effects of genetic and intrauterine environments on neonatal microbial communities and to explore selective FGR-induced gut microbiota disruption, metabolic profile disturbances and possible outcomes. DESIGN: We profiled and compared the gut microbial colonisation of 150 pairs of twin neonates who were classified into four groups based on their chorionicity and discordance of fetal birth weight. Gut microbiota dysbiosis and faecal metabolic alterations were determined by 16S ribosomal RNA and metagenomic sequencing and metabolomics, and the long-term effects were explored by surveys of physical and neurocognitive development conducted after 2~3 years of follow-up. RESULTS: Adverse intrauterine environmental factors related to selective FGR dominate genetics in their effects of elevating bacterial diversity and altering the composition of early-life gut microbiota, and this effect is positively related to the severity of selective FGR in twins. The influence of genetic factors on gut microbes diminishes in the context of selective FGR. Gut microbiota dysbiosis in twin neonates with selective FGR and faecal metabolic alterations features decreased abundances of Enterococcus and Acinetobacter and downregulated methionine and cysteine levels. Correlation analysis indicates that the faecal cysteine level in early life is positively correlated with the physical and neurocognitive development of infants. CONCLUSION: Dysbiotic microbiota profiles and pronounced metabolic alterations are associated with selective FGR affected by adverse intrauterine environments, emphasising the possible effects of dysbiosis on long-term neurobehavioural development.

Alcohol Pretreatment to Eliminate the Interference of Micro Additive Particles in the Identification of Microplastics Using Raman Spectroscopy.

Raman spectroscopy is an indispensable tool in the analysis of microplastics smaller than 20 µm. However, due to its limitation, Raman spectroscopy may be incapable of effectively distinguishing microplastics from micro additive particles. To validate this hypothesis, we characterized and compared the Raman spectra of six typical slip additives with polyethylene and found that their hit quality index values (0.93-0.96) are much higher than the accepted threshold value (0.70) used to identify microplastics. To prevent this interference, a new protocol involving an alcohol treatment step was introduced to successfully eliminate additive particles and accurately identify microplastics. Tests using the new protocol showed that three typical plastic products (polyethylene pellets, polyethylene bottle caps, and polypropylene food containers) can simultaneously release microplastic-like additive particles and microplastics regardless of the plastic type, daily-use scenario, or service duration. Micro additive particles can also adsorb onto and modify the surfaces of microplastics in a manner that may potentially increase their health risks. This study not only reveals the hidden problem associated with the substantial interference of additive particles in microplastic detection but also provides a cost-effective method to eliminate this interference and a rigorous basis to quantify the risks associated with microplastic exposure.

Microbe-Based Sensor for Long-Term Detection of Urine Glucose.

The development of a reusable and low-cost urine glucose sensor can benefit the screening and control of diabetes mellitus. This study focused on the feasibility of employing microbial fuel cells (MFC) as a selective glucose sensor for continuous monitoring of glucose levels in human urine. Using MFC technology, a novel cylinder sensor (CS) was developed. It had a quick response time (100 s), a large detection range (0.3-5 mM), and excellent accuracy. More importantly, the CS could last for up to 5 months. The selectivity of the CS was validated by both synthetic and actual diabetes-negative urine samples. It was found that the CS's selectivity could be significantly enhanced by adjusting the concentration of the culture's organic matter. The CS results were comparable to those of a commercial glucose meter (recovery ranged from 93.6% to 127.9%) when the diabetes-positive urine samples were tested. Due to the multiple advantages of high stability, low cost, and high sensitivity over urine test strips, the CS provides a novel and reliable approach for continuous monitoring of urine glucose, which will benefit diabetes assessment and control.

Potential of using synthesized nano-zeolite for ammonium and phosphate immobilization in dairy wastewater.

The studies described in the Research Communication aimed to describe the feasibility of using coal fly ash to synthesize nano-zeolite, and the ammonium and phosphate adsorption efficiencies of the nanomaterial in dairy wastewater. Chemical treatment of coal fly ash was conducted and changes observed. Samples treated with NaOH had an increased cation exchange capacity and P sorption index compared to the initial fly ash, due to particle modification from smooth surface to plate- and rod-shape crystals, referred to as nano-zeolite. Batch experiments were conducted by mixing coal fly ash and nano-zeolite with synthesized wastewater to study the effect of sorption time, pH values and dosage of nano-zeolite on ammonium and phosphate removal efficiency. The adsorption process reached equilibrium in a very short time (less than 60 min), which suggests a potential for fast immobilization of pollutants. The concentration of ammonium decreased from 118 to 35 mg/l (71% removal) while the concentration of phosphate decreased from 52 to 45 mg/l. The removal efficiency of ammonium was 36·6, 51·8 and 70·9% at pH 3, 7 and 10, respectively whilst that of phosphate increased dramatically with decreased slurry pH (92·1, 47·3 and 12·3% at pH 3, 7 and 10, respectively). Nano-zeolite could be a potential absorbent for fast immobilization of ammonium but not phosphate. Surface modification of nano-zeolite could be introduced in order to enhance the pollutants removal efficiency.

DairyWater: striving for sustainability within the dairy processing industry in the Republic of Ireland.

This Review describes the objectives and methodology of the DairyWater project as it aims to aid the Irish dairy processing industry in achieving sustainability as it expands. With the abolition of European milk quotas in March 2015, the Republic of Ireland saw a surge in milk production. The DairyWater project was established in anticipation of this expansion of the Irish dairy sector in order to develop innovative solutions for the efficient management of water consumption, wastewater treatment and the resulting energy use within the country's dairy processing industry. Therefore, the project can be divided into three main thematic areas: dairy wastewater treatment technologies and microbial analysis, water re-use and rainwater harvesting and environmental assessment. In order to ensure the project remains as relevant as possible to the industry, a project advisory board containing key industry stakeholders has been established. To date, a number of large scale studies, using data obtained directly from the Irish dairy industry, have been performed. Additionally, pilot-scale wastewater treatment (intermittently aerated sequencing batch reactor) and tertiary treatment (flow-through pulsed ultraviolet system) technologies have been demonstrated within the project. Further details on selected aspects of the project are discussed in greater detail in the subsequent cluster of research communications.

National scale assessment of total trihalomethanes in Irish drinking water.

Ireland reported the highest non-compliance with respect to total trihalomethanes (TTHMs) in drinking water across the 27 European Union Member States for the year 2010. We carried out a GIS-based investigation of the links between geographical parameters and catchment land-uses with TTHMs concentrations in Irish drinking water. A high risk catchment map was created using peat presence, rainfall (>1400 mm) and slope (<5%) and overlain with a map comprising the national dataset of routinely monitored TTHM concentrations. It appeared evident from the map that the presence of peat, rainfall and slope could be used to identify catchments at high risk to TTHM exceedances. Furthermore, statistical analyses highlighted that the presence of peat soil with agricultural land was a significant driver of TTHM exceedances for all treatment types. PARAFAC analysis from three case studies identified a fluorophore indicative of reprocessed humic natural organic matter as the dominant component following treatment at the three sites. Case studies also indicated that (1) chloroform contributed to the majority of the TTHMs in the drinking water supplies and (2) the supply networks contributed to about 30 µg L-1 of TTHMs.

Sedation effects of intranasal dexmedetomidine delivered as sprays versus drops on pediatric response to venous cannulation.

OBJECTIVES: Mucosal atomization device (MAD) was designed to increase the bioavailability of intranasal medications by facilitating absorption, the present study aimed to evaluate and compare the sedation effects of intranasal dexmedetomidine delivered as drops versus sprays on pediatric responses to intravenous cannulation. METHODS: One hundred and six pediatric patients (aged from 2 to 5years) scheduled for elective ophthalmic surgery were intranasally received a dose of 2µg/kg in 20µl/kg of dexmedetomidine for sedation to reduce response to venous cannulation. The patients were randomized into syringe group and MAD group in which dexmedetomidine was delivered as drops or sprays via syringe or MAD respectively. The primary outcome was the response to peripheral vein cannulation assessed by the FLACC scores (faces, legs, activity, cry and consolability) 30min after intranasal administration of dexmedetomidine. The secondary outcomes included acceptance for intranasal medication, sedation onset time, and needle insertion times and any adverse event at the preoperative holding area. RESULTS: The FLACC scores in MAD group were significantly decreased than that treated by drops (P=0.021). The acceptance for intranasal administration between both groups was comparable (P>0.05), the onset time and the incidences in two and more times of needle insertion did not differ significantly between syringe and MAD groups (all P>0.05). None of patients were required to clinically intervene in heart rates reduction and none suffered respiratory depression after administrations of dexmedetomidine in either group. CONCLUSION: Intranasal dexmedetomidine by sprays offers better sedation effects to reduce responses to venous cannulation than drops.

Forest clearfelling effects on dissolved oxygen and metabolism in peatland streams.

Peatlands cover ∼3% of the world's landmass and large expanses have been altered significantly as a consequence of land use change. Forestry activities are a key pressure on these catchments increasing suspended sediment and nutrient export to receiving waters. The aim of this study was to investigate stream dissolved oxygen (DO) and metabolic activity response following clearfelling of a 39-year-old lodgepole pine and Sitka spruce forestry in an upland peat catchment. Significant effects of clearfelling on water temperature, flows, DO and stream metabolic (photosynthesis, respiration) rates were revealed. Stream temperature and discharge significantly increased in the study stream following clearfelling. Instream ecosystem respiration increased significantly following clearfelling, indicating an increase in the net consumption of organic carbon.

Ptaquiloside in Irish Bracken Ferns and Receiving Waters, with Implications for Land Managers.

Ptaquiloside, along with other natural phytotoxins, is receiving increased attention from scientists and land use managers. There is an urgent need to increase empirical evidence to understand the scale of phytotoxin mobilisation and potential to enter into the environment. In this study the risk of ptaquiloside to drinking water was assessed by quantifying ptaquiloside in the receiving waters at three drinking water abstraction sites across Ireland and in bracken fronds surrounding the abstraction sites. We also investigated the impact of different management regimes (spraying, cutting and rolling) on ptaquiloside concentrations at plot-scale in six locations in Northern Ireland, UK. Ptaquiloside concentrations were determined using recent advances in the use of LC-MS for the detection and quantification of ptaquiloside. The results indicate that ptaquiloside is present in bracken stands surrounding drinking water abstractions in Ireland, and ptaquiloside concentrations were also observed in the receiving waters. Furthermore, spraying was found to be the most effective bracken management regime observed in terms of reducing ptaquiloside load. Increased awareness is vital on the implications of managing land with extensive bracken stands.

Utilisation of potato processing wastewater for microbial lipids and γ-linolenic acid production by oleaginous fungi.

BACKGROUND: Microbial lipids are considered as the starting material for production of second-generation biofuels and their polyunsaturated fatty acids are rich sources of neutraceuticals. Exploring cheap feedstock for producing microbial lipids is necessary. The present study examined the potential of microbial lipids and γ-linolenic acid (GLA) production by two oleaginous fungi, Aspergillus flavus I16-3 and Mucor rouxii, with potato processing wastewater as a low-cost or no-cost nutrient source. RESULTS: Biochemistry and physiology of two oleaginous fungi, A. flavus I16-3 and M. rouxii, on lipid accumulation showed the two fungi grew well and efficiently utilised the starch in wastewater. On average (P < 0.05), 2.8 and 3.6 g L(-1) of lipids were produced by A. flavus I16-3 and M. rouxii, respectively, with maximum GLA yields of 60 and 100 mg L(-1) . Addition of nutrients to raw wastewater significantly improved (P < 0.05) the lipid and GLA yields; 3.5 and 4.2 g L(-1) of lipids, and 100 and 140 mg L(-1) of GLA were produced by A. flavus I16-3 and M. rouxii, respectively. In addition, the wastewater was efficiently treated, with soluble chemical oxygen demand, total soluble nitrogen and total soluble phosphorus removals up to 60% and 90%, 100% and 98%, and 92% and 81% by A. flavus I16-3 and M. rouxii, respectively. CONCLUSION: This study demonstrated an alternative approach to valorise potato processing wastewater to produce microbial lipids and GLA (nutraceuticals).

Microbiome in Female Reproductive Health: Implications for Fertility and Assisted Reproductive Technologies.

The microbiome plays a critical role in the process of conception and the outcomes of pregnancy. Disruptions in microbiome homeostasis in women of reproductive age can lead to various pregnancy complications, which significantly impact maternal and fetal health. Recent studies have associated the microbiome in the female reproductive tract (FRT) with assisted reproductive technology (ART) outcomes, and restoring microbiome balance has been shown to improve fertility in infertile couples. This review provides an overview of the role of the microbiome in female reproductive health, including its implications for pregnancy outcomes and ARTs. Additionally, recent advances in the use of microbial biomarkers as indicators of pregnancy disorders are summarized. A comprehensive understanding of the characteristics of the microbiome before and during pregnancy and its impact on reproductive health will greatly promote maternal and fetal health. Such knowledge can also contribute to the development of ARTs and microbiome-based interventions.

Machine learning-assisted assessment of key meteorological and crop factors affecting historical mulch pollution in China.

Degraded mulch pollution is of a great concern for agricultural soils. Although numerous studies have examined this issue from an environmental perspective, there is a lack of research focusing on crop-specific factors such as crop type. This study aimed to explore the correlation between meteorological and crop factors and mulch contamination. The first step was to estimate the amounts of mulch-derived microplastics (MPs) and phthalic acid esters (PAEs) during the rapid expansion period (1993-2012) of mulch usage in China. Subsequently, the Elastic Net (EN) and Random Forest (RF) models were employed to process a dataset that included meteorological, crop, and estimation data. At the national level, the RF model suggested that coldness in fall was crucial for MPs generation, while vegetables acted as a key factor for PAEs release. On a regional scale, the EN results showed that crops like vegetables, cotton, and peanuts remained significantly involved in PAEs contamination. As for MPs generation, coldness prevailed over all regions. Aridity became more critical for southern regions compared to northern regions due to solar radiation. Lastly, each region possessed specific crop types that could potentially influence its MPs contamination levels and provide guidance for developing sustainable ways to manage mulch contamination.

Characteristics of zero-valent iron surface oxide films under the catalytic interface reactions by assisting ligands in nitrate-contaminated groundwater.

The surface passivation layer coating on zero-valent iron (ZVI) particles impedes the electron transfer from ZVI to nitrate. To enhance the efficiency of nitrate reduction by Fe(0), we tested the chemical process and the thickness of the iron oxide film on the surface of Fe(0) particles, utilizing Fe2+aq in aqueous solution and wheat straw as ligands. A novel principal surface catalyzing reaction was formulated as follows: [Formula: see text] . When Fe2+aq concentration increased from 0 - 200 mg·L-1, the NO3- removal rate increased from 6.95% to 82.6% respectively during 12 h and it was 48%, 72%, 79% and 94% respectively in Fe0/WS ratio of 0, 0.25, 0.5 and 1 system. Uniform surface iron oxide films formed around the Fe(0) particles within 12 h after the adding Fe2+aq or wheat straw to the Fe(0) system. The composition and thickness of these films were dependent on the quantity of added materials. X-ray diffraction (XRD) analysis revealed that surface oxide iron mainly consisted of Fe2+ or Fe3+ oxides, with Fe3O4 being predominant. The X-ray photoelectron spectroscopy (XPS) etching indicated that the addition of Fe(0)/straw at mass ratios of 1 or system with 20 mg·L-1 Fe2+aq resulted in the thinnest surface iron oxide layer. The study demonstrated that reducing the oxide layer's thickness was achieved through partial catalysis and enhanced complexation capacity. This reduction was facilitated by the introduction of Fe2+aq or wheat straw into the Fe(0) system, potentially improving proton dissociation and promoting the ligand-assisted dissolution of Fe3+ oxides.

Spatiotemporal patterns of the pregnancy microbiome and links to reproductive disorders.

The microbiome of females undergoes extensive remodeling during pregnancy, which is likely to have an impact on the health of both mothers and offspring. Nevertheless, large-scale integrated investigations characterizing microbiome dynamics across key body habitats are lacking. Here, we performed an extensive meta-analysis that compiles and analyzes microbiome profiles from  >10,000 samples across the gut, vagina, and oral cavity of pregnant women from diverse geographical regions. We have unveiled unexpected variations in the taxonomic, functional, and ecological characteristics of microbial communities throughout the course of pregnancy. The gut microbiota showed distinct trajectories between Western and non-Western populations. The vagina microbiota exhibited fluctuating transitions at the genus level across gestation, while the oral microbiota remained relatively stable. We also identified distinctive microbial signatures associated with prevalent pregnancy-related disorders, including opposite variations in the oral and gut microbiota of patients with gestational diabetes and disrupted microbial networks in preterm birth. This study establishes a comprehensive atlas of the pregnancy microbiome by integrating multidimensional datasets and offers foundational insights into the intricate interplay between microbes and host factors that underlie reproductive health.