Fabrication of biologically inspired electrospun collagen/silk fibroin/bioactive glass composited nanofibrous to accelerate the treatment efficiency of wound repair.

A triple-layer matrix Collagen/Silk fibroin/Bioactive glass composited Nanofibrous was fabricated by linking electrospinning and freeze-drying systems, this typical three layered composite with a nanofibrous fragment as the key (top) layer, middle portion as inferior, and a spongy porous fragment as the third (bottom) deposit to develop the synergistic effect of composite materials resultant to physical and biological performances. Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy were used to assess the final material's physicochemical properties (SEM). The triple-layer matrix had a nanofibrous and porous structure, which has qualities including high porosity, swelling, and stability, which are important in soft-tissue engineering. NIH 3 T3 fibroblast and humanoid keratinocyte (HaCaT) cell lines were also used to investigate the matrix's in vitro biological and fluorescent capabilities, which showed excellent cell adherence and proliferation across the composite layers. The synergistic arrangement of nanofibrous substantial deposition onto collagenous with silk fibroin candidates has therefore proven effective in the construction of a tri-layer matrix for skin-tissue-engineering applications.

Phytochemical analysis and antibacterial activity of Gentiana macrophylla extract against bacteria isolated from burn wound infections.

The management of burn wound victims is often considered a difficult task due to frequent infections. Moreover, the treatment with conventional antibiotics is associated with the upsurge of drug resistant bacterial strains. Therefore there is pressing need to explore antimicrobials that could overcome these problems. Plant extracts have been used as antimicrobials in different systems of medicine. Gentiana species have been widely used in Chinese system of traditional medicine for the treatment of various ailments including microbial infections. The aim of the present study was therefore to evaluate the antimicrobial activity of methanolic root extract of Gentiana macrophylla against bacterial strains isolated from wound burns. Preliminary phytochemical screening revealed the phenolic and flavonoids content of the extract to be 26.70 ± 1.5 mg gallic acid equivalent (GAE)/g DW and 10.11 ± 0.8 mg quercetin equivalent/g DW respectively. The phenolics and flavonoids content of the extract positively correlated with antioxidant activity of the extract. Evaluation of antimicrobial activity against bacterial strains isolated from wound burns revealed that the G. macrophylla extract exhibited significant antibacterial against all the tested bacterial strains. The MIC values ranged between 60 and 240 µg/ml. The lowest MIC was observed for S. epidermidis and the highest MIC was observed for E. coli. The MBCs also followed the similar trend and ranged between 106 to 300 µg/ml. To identify the active constituents responsible for the bioactivity of the extract LC/MS analysis was carried out which lead to the putative identification of five compounds including chlorogenic acid, rutin, sweroside, isogentisin, and gentisin. Taken together, these results indicate G. macrophylla can prove to be an important source of antioxidants and antimicrobials and may be used for the management of wound burns.

Fabrication of homogeneously-aligned nano-fillers encapsulated silk fibroin electrospun nanofibers for improved fibroblast attachment, epithelialization, and collagen depositions: in vitro and in vivo wound healing evaluation.

Curcumin (CUR), a natural compound found in turmeric that has multiple biological functions such as antibacterial, anti-oxidant, anti-cancer, and wound healing properties due to its hydrophobicity CUR solubilization is a great challenge. In this study, the electrospinning process is used to fabricate a novel active wound dressing based on CUR loaded silk fibroin (SF)/hydroxyapatite (HAp) (SF/HAp-CUR) nanofibers in diabetic rats. The incorporation of CUR into the SF/HAp-CUR nanofibers had an obvious effect on the morphology and dimension of SF/HAp-CUR nanofibers characterized by SEM analysis. Morphological analysis revealed that the average fiber diameter of the SF/HAp, SF/HAp-CUR(1.0%), SF/HAp-CUR(3.0%), and SF/HAp-CUR(5.0%) nanofibers were calculated to be 461 ± 65 nm, 323 ± 90 nm, 412 ± 110, and 497 ± 118 nm. In addition of CUR in the SF/HAp nanofibers significantly improved the mechanical properties in terms of enhanced elongation at break and tensile strengths. The percentages of water uptake and porosity of SF/HAp-CUR nanofibers were 143.7 ± 4.05% and 92.5 ± 3.40%, respectively. The results showed that CUR presented a sustained release behavior from SF/HAp-CUR nanofibers and maintained its free radical scavenging ability. The prepared nanofibers surface interaction was confirmed by FT-IR and XRD analysis. Antibacterial tests revealed SF/HAp-CUR on day 14 improved the bacterial embarrassment of both E. coli and S. aureus by 4 to 5-fold, respectively. The cell cytotoxicity with L929 mouse fibroblasts on the SF/HAp-CUR nanofibers was very low at 7.7 ± 1.75% on day 14. In vivo wound healing showed that the treatment using SF/HAp-CUR nanofibers significantly increased the rate of wound closure (99.6 ± 0.86%) on day 21 compared with that using SF/HAp nanofibers (67.7 ± 4.25%). These results showed that the delivery of SF/HAp-CUR nanofibers can facilitate antibacterial, anti-oxidant, cytotoxicity of wound healing properties.