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The present study was conducted to assess rumen bacteria in lactating cows with different milk protein yield, aiming to understand the role of rumen bacteria in this trait. Cows with high milk protein yield (high milk yield and high milk protein content, HH; n = 20) and low milk protein yield (low milk yield and low milk protein content, LL; n = 20) were selected from 374 mid-lactation Holstein dairy cows fed a high-grain diet. Measurement of the rumen fermentation products showed that the concentrations of ruminal total volatile fatty acids, propionate, butyrate, and valerate and the proportion of isobutyrate were higher in the HH cows than in the LL cows. Amplicon sequencing analysis of the rumen bacterial community revealed that the richness (Chao 1 index) of rumen microbiota was higher in the LL cows than in the HH cows. Among the 10 predominant bacterial phyla (relative abundance being >0.10%, present in >60% of animals within each group), the relative abundance of Proteobacteria was 1.36-fold higher in the HH cows than in the LL cows. At the genus level, the relative abundance of Succinivibrio was significantly higher and that of Clostridium tended to be higher in the LL cows than in the HH cows. Sharpea was 2.28-fold enriched in the HH cows compared with the LL cows. Different relationships between the relative abundances of rumen microbial taxa and volatile fatty acid concentrations were observed in the HH and the LL animals, respectively. Succinivibrio and Prevotella were positively correlated with acetate, propionate, and valerate in the LL cows, whereas Sharpea was positively correlated with propionate and valerate concentrations in the HH cows. Collectively, our results revealed that rumen bacterial richness and the relative abundances of several bacterial taxa significantly differed between dairy cows with high and low milk protein yields, suggesting the potential roles of rumen microbiota contributing to milk protein yield in dairy cows.
Assuntos
Bactérias/metabolismo , Proteínas do Leite/análise , Leite/química , Rúmen/microbiologia , Animais , Butiratos/análise , Bovinos , Indústria de Laticínios , Dieta/veterinária , Ácidos Graxos Voláteis/análise , Feminino , Fermentação , Lactação , Prevotella/metabolismo , Propionatos/metabolismo , Rúmen/metabolismo , Valeratos/metabolismoRESUMO
Objective: To explore the effects of pedicled flap combined with membrane induction technique in repairing foot and ankle wounds in diabetic patients. Methods: A retrospective observational study was conducted. From March 2019 to July 2021, 12 patients with diabetic foot and ankle wounds who met the inclusion criteria were admitted to Wuxi Ninth People's Hospital, including 7 males and 5 females, aged 20 to 92 years. The wound area before debridement was 4.0 cm×2.5 cm to 16.0 cm×12.5 cm. The patients underwent debridement+antibiotic cement tamponade in stage â ; according to the wound site, peroneal artery perforator flap or posterior tibial artery perforator flap was chosen to repair the wound in stage â ¡, with the area of the resected flap ranging from 4.5 cm×3.0 cm to 18.5 cm×14.0 cm. The donor site was directly closed in 4 patients or covered by full-thickness inguinal skin graft in 8 patients. After the operation of stage â ¡, the survival of flap and skin graft, the scar in donor and recipient sites of flap, the appearance of flap, and the function of ankle joint of affected extremity were followed up. The recovery of foot and ankle function was evaluated and rated by the American Orthopaedic Foot and Ankle Society (AOFAS) Ankle-Hindfoot Scoring System at the last follow-up. Results: During the follow-up of 4 to 15 months after the operation of stage â ¡, both the flap and skin graft survived, without obvious infection recurrence. Linear scars were left in donor and recipient sites of flap, with good appearance in flap. The function of ankle joint in the affected extremity was nearly normal. At the last follow-up, the AOFAS scores of patients were 79 to 93, with excellent in 8 cases and good in 4 cases. Conclusions: The pedicled flap combined with membrane induction technique for repairing foot and ankle wounds in diabetic patients has the advantage of simple operation, preserved ankle joint function, and less postoperative infection recurrence, which is worth popularizing in clinical practice.
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Diabetes Mellitus , Pé Diabético , Retalho Perfurante , Procedimentos de Cirurgia Plástica , Lesões dos Tecidos Moles , Masculino , Feminino , Humanos , Tornozelo/cirurgia , Articulação do Tornozelo/cirurgia , Lesões dos Tecidos Moles/cirurgia , Transplante de Pele , Extremidade Inferior , Retalho Perfurante/irrigação sanguínea , Cicatriz/cirurgia , Pé Diabético/cirurgia , Resultado do TratamentoRESUMO
Objective: To explore the clinical efficacy of antibiotic bone cement combined with vacuum sealing drainage (VSD) in treating diabetes mellitus complicated with necrotizing fasciitis. Methods: The retrospective observational study approach was used. From January 2020 to March 2022, 12 patients with type 2 diabetes complicated with necrotizing fasciitis who met the inclusion criteria were admitted to Wuxi Ninth People's Hospital, including 7 males and 5 females, aged 27 to 76 years. The initial diagnosis of lesions was in the lower limbs. After admission, bedside incision and drainage were performed timely, and a sample of wound exudate was collected for microbial cultivation. At the same time, the comprehensive supportive treatment was performed. At stage â , debridement was performed, and the skin and soft tissue defect area was 40 cm×15 cm to 80 cm×25 cm after debridement. The dead space was filled with bone cement containing gentamicin and vancomycin and VSD was performed. After there was no obvious infection on the wound, the antibiotic bone cement was removed and wound repair surgery was performed at stage â ¡. The times of debridement, amputation, infection control, wound treatment method and wound healing at stage â ¡, total hospitalization day, and recurrence of necrotizing fasciitis during follow-up after the stage â ¡ surgery. At the last follow-up, the walking function of patients was evaluated according to the scoring standards of American Orthopedic Foot and Ankle Association (AOFAS). Results: Eleven patients had wound infection control with one debridement surgery and did not undergo amputation surgery; one patient had significant foot gangrene, and the infection was controlled after one debridement and amputation of the gangrenous limb. Blood routine and infection indicators gradually returned to normal within 7 days after surgery. At stage â ¡, the wounds in 4 patients were sutured directly, the wounds in 6 patients were repaired with full-thickness inguinal skin graft, while the wounds in 2 patients were repaired with pedicled or tongue-shaped flaps at the wound edge. The wounds healed well after surgery, with no ulceration. The total hospitalization day of patients was 20 to 45 days. Follow-up for 3 to 24 months after stage â ¡ surgery showed no recurrence of necrotizing fasciitis in any patient. At the last follow-up, the walking function was evaluated as excellent in 10 cases and good in 2 cases according to the AOFAS scoring standard. Conclusions: Antibiotic bone cement combined with VSD used in treating type 2 diabetes complicated with necrotizing fasciitis can effectively control infection and reduce the times of debridement, with good wound healing and walking function after surgery.
Assuntos
Diabetes Mellitus Tipo 2 , Fasciite Necrosante , Tratamento de Ferimentos com Pressão Negativa , Lesões dos Tecidos Moles , Feminino , Humanos , Masculino , Antibacterianos/uso terapêutico , Cimentos Ósseos/uso terapêutico , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/cirurgia , Drenagem , Fasciite Necrosante/cirurgia , Extremidade Inferior , Tratamento de Ferimentos com Pressão Negativa/métodos , Transplante de Pele , Lesões dos Tecidos Moles/cirurgia , Resultado do Tratamento , Adulto , Pessoa de Meia-Idade , IdosoRESUMO
Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, "Long non-coding RNA CASC15 promotes nasopharyngeal carcinoma cell proliferation and metastasis by downregulating miR-101-3p, by M.-Y. Xue, H.-X. Cao, published in Eur Rev Med Pharmacol Sci 2019; 23 (20): 8897-8904-DOI: 10.26355/eurrev_201910_19285-PMID: 31696476" has been withdrawn. The Publisher apologizes for any inconvenience this may cause. https://www.europeanreview.org/article/19285.
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OBJECTIVE: Previous studies have shown that long intergenic non-coding RNA01551 (LINC01551) is a cancer-promoting gene. However, the role of LINC01551 in nasopharyngeal carcinoma (NPC) has not been reported. Therefore, the aim of this study was to investigate the expression characteristics of LINC01551 in NPC, and to further explore its mechanism in promoting the metastasis. PATIENTS AND METHODS: Quantitative real time-polymerase chain reaction (qRT-PCR) was used to detect the expression levels of LINC01551 in tumor tissue samples and paracancerous normal ones of 36 patients with NPC; meanwhile, the expression of LINC01551 in NPC cell lines was also verified using the qRT-PCR assay. In addition, the LINC01551 knockdown model was constructed in NPC cell lines (CNE2 and 6-10B) using lentivirus, and the influence of LINC01551 on the function of NPC cells was analyzed by cell counting kit-8 (CCK-8) and transwell invasion assays. Finally, the interaction between LINC01551 and microRNA-132-5p was examined by Luciferase reporter gene assay, while the potential mechanism was further explored by cell reverse experiments. RESULTS: The results of qRT-PCR indicated that the expression level of LINC01551 in tumor tissue specimens of these patients was remarkably higher than that in adjacent tissues, and the difference was statistically significant. Meanwhile, LINC01551 expression was also found remarkably higher in cell lines than that in normal ones. In addition, compared with blank or control group, the proliferation, invasion and metastasis ability of NPC cells in LINC01551 knockdown group (si-LINC01551) was significantly reduced. Subsequently, the result of Luciferase reporting assay demonstrated that overexpression of microRNA-132-5p attenuated the Luciferase activity of the wild-type LINC01551 vector without attenuating that of the mutant vector, further demonstrating that LINC01551 can be combined with miR-132-5p. Additionally, the result of cell reverse experiment revealed that knockdown of microRNA-132-5p could reverse the effect of LINC01551 silencing on proliferation rate and metastasis of NPC cells, thus further demonstrating the mutual regulation between LINC01551 and microRNA-132-5p. CONCLUSIONS: The above studies indicated that LINC01551 was remarkably up-regulated in NPC tissues, as well as in cell lines. In addition, studies have shown that LINC01551 may promote the metastatic ability by regulating microRNA-132-5p.
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MicroRNAs/metabolismo , Carcinoma Nasofaríngeo/metabolismo , Neoplasias Nasofaríngeas/metabolismo , RNA Longo não Codificante/metabolismo , Linhagem Celular , Proliferação de Células , Humanos , MicroRNAs/genética , Carcinoma Nasofaríngeo/patologia , Neoplasias Nasofaríngeas/patologia , RNA Longo não Codificante/genéticaRESUMO
OBJECTIVE: Nasopharyngeal carcinoma (NPC) is one of the most common malignant tumors worldwide. Recent studies have revealed that long non-coding RNAs (lncRNAs) play important roles in the progression of tumorigenesis. The aim of this study was to identify the exact role of lncRNA CASC15 in the progression of NPC. PATIENTS AND METHODS: CASC15 expression in both 54 paired NPC patients' tissue samples and cell lines was detected by Real Time-quantitative Polymerase Chain Reaction (RT-qPCR). Moreover, the function of CASC15 was identified by performing cell proliferation assay, transwell assay and wound healing assay in vitro. The underlying mechanism was explored through Luciferase assay and RT-qPCR. In addition, tumor formation and metastasis assays were conducted in vivo. RESULTS: CASC15 expression in NPC tissues was markedly higher than that of adjacent non-tumor tissues. The proliferation, migration and invasion of NPC cells were significantly inhibited after knockdown of CASC15 in vitro. Our further experiments revealed that miR-101-3p was remarkably up-regulated via knockdown of CASC15. Meanwhile, miR-101-3p was a direct target of CASC15 in NPC. Furthermore, tumor formation and metastasis of NPC were significantly inhibited via knockdown of CASC15 in nude mice. CONCLUSIONS: CASC15 enhances NPC cell proliferation and metastasis via sponging miR-101-3p in vitro and in vivo.