RESUMO
Three new compounds, namely massonside C (1), massonianoside F (2), and 3, 8-dimethyl- herbacetin-7-O-ß-D-glucopyranoside (3), together with five known compounds (4-8), were isolated from the fresh needles of Pinus massoniana. Their structures were established by 1D, 2D NMR, HRMS and comparison with the literature data. The absolute configuration of 1 was confirmed by a combination of X-ray single crystal analysis. All isolated compounds were evaluated for the protective effect of human umbilical vein endothelial cells against oxidative damage.
Assuntos
Diterpenos , Lignanas , Pinus , Células Endoteliais , Flavonoides , Humanos , Estrutura Molecular , Folhas de Planta , Raios XRESUMO
Based on variation of Pinus massoniana families, heritablility and correlation analysis, the contents of shikimic acid and procyanidine (heritability 0.90, 0.70), dry weight of single branch (heritability 0.60) and and leaf length (heritability 0.46) were screened out as quality, yield and harvest cost traits of Folium Pini, respectively. For the different medicinal application of Folium Pini, varied methods were chosen to estimate weight and construct index equation. Weight adjustment based.on equal emphasis were used as economic weight determining method to select the best families, and the index (accuracy 0. 936 4 and heritability 0. 881 6) obtained was a little better than that obtained by equal emphasis, and much better than that by restricted index. The superior families selected with adjustment weight and equal emphasis were No. 46, 43 and 28. Partial regression were used as economic weight determining method to select the best families,and the index obtained had the highest accuracy (0.941 5) , index heritability (0. 889 9) and the genetic gain of shikimic acid content. The superior families selected with this method were No. 46, 27 and 47. No. 46 was the best families with maximal economic benefit. Our study indicated that suitable method for estimate weight and construct index equation can be applied for better accuracy of superior families selection of P. massoniana.
Assuntos
Medicamentos de Ervas Chinesas/análise , Pinus/química , Plantas Medicinais/química , Cruzamento , Pinus/classificação , Pinus/genética , Pinus/crescimento & desenvolvimento , Folhas de Planta/química , Plantas Medicinais/classificação , Plantas Medicinais/genética , Plantas Medicinais/crescimento & desenvolvimentoRESUMO
OBJECTIVE: To study the factors that affect the quality of Rhei Radix et Rhizoma, including those from different regions, of different specifications and different growth patterns. METHODS: Detect the contents of extract, total ash, acid-insoluble ash and active ingredients of Rhei Radix et Rhizoma from 18 different sources. The results were compared by SPSS software and DTOPSIS. RESULTS: The quality of different sources was studied from the contents of extract, total ash and content of five active ingredients. The first-class of Rhei Radix et Rhizoma was from Songpan, Sichuan, and the second-class was from Changdu, Xizang. The wild Rhei Radix et Rhizoma was superior in the contents of extract and total ash compared with the cultivated one. In terms of ash content, "shuigen" was inferior to the integration and the selection. CONCLUSION: DTOPSIS method can be effectively applied to source screening and quality control of commercial medicinal materials of Rheum palmatum.
Assuntos
Medicamentos de Ervas Chinesas/normas , Controle de Qualidade , Rheum/química , Raízes de Plantas/química , Rizoma/químicaRESUMO
Isolariciresinol-9'-O-α-L-arabinofuranoside (MWS19) isolated from Pinus massoniana Lamb. Fresh pine needles is the major ingredient of the Songling Xuemaikang capsule therapy used for hypertension. The present study aimed to investigate the effects and underlying mechanisms of MWS19 on hydrogen peroxide (H2O2)induced apoptosis in human umbilical vein endothelial cells (HUVECs). To investigate the effect of MWS19 on apoptosis in HUVECs, an oxidative stressinduced apoptosis model was established in HUVECs using H2O2, and the present study performed Hoechst 33258 staining and a Cell Counting Kit8 (CCK8) assay. Furthermore, western blot analysis was also performed to investigate the underlying mechanism of the effects of MWS19 on the model. The results demonstrated that MWS19 reversed the effects of H2O2 on cell apoptosis at a concentration range of 15.6250 µg/ml, with dosedependent increases in cell growth. Hoechst staining indicated that 500 µM H2O2 induced HUVEC apoptosis, and MWS19 markedly protected HUVECs against apoptosis at 31.3, 62.5 and 125 µg/ml. Furthermore, the protein expression of phosphatidylinositol 3kinase (PI3K), phosphorylatedAkt and Bcl2associated agonist of cell death (Bad) were increased, and reduced caspase3 activation was observed, following treatment with MWS19 in H2O2treated HUVECs. Additionally, the PI3K inhibitor wortmannin attenuated PI3K/Akt/Bad signaling induced by MWS19 treatment and neutralized the effect of MWS19 on the growth of HUVECs. In conclusion, the results of the present study indicate that MWS19 may protect against H2O2induced HUVEC apoptosis via the PI3K/Akt/Bad signaling pathway. MWS19 may serve an important role in the prevention of oxidative damage in vascular endothelial cells in hypertension patients.
Assuntos
Apoptose/efeitos dos fármacos , Peróxido de Hidrogênio/farmacologia , Fosfatidilinositol 3-Quinase/metabolismo , Extratos Vegetais/farmacologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais/efeitos dos fármacos , Proteína de Morte Celular Associada a bcl/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Células Endoteliais da Veia Umbilical Humana , HumanosRESUMO
Two new sesquiterpene glycosides, namely massonside A (1) and massonside B (2), were isolated from the n-Bu extract of the fresh needles of Pinus massoniana Lamb. Their structures were established by 1D, 2D nuclear magnetic resonance and high-resolution mass spectrometry. Their biological activities were profiled by the anti-HBV and anti-HCV assays.