Clinical factors associated with composition of lung microbiota and important taxa predicting clinical prognosis in patients with severe community-acquired pneumonia.

Few studies have described the key features and prognostic roles of lung microbiota in patients with severe community-acquired pneumonia (SCAP). We prospectively enrolled consecutive SCAP patients admitted to ICU. Bronchoscopy was performed at bedside within 48 h of ICU admission, and 16S rRNA gene sequencing was applied to the collected bronchoalveolar lavage fluid. The primary outcome was clinical improvements defined as a decrease of 2 categories and above on a 7-category ordinal scale within 14 days following bronchoscopy. Sixty-seven patients were included. Multivariable permutational multivariate analysis of variance found that positive bacteria lab test results had the strongest independent association with lung microbiota (R2 = 0.033; P = 0.018), followed by acute kidney injury (AKI; R2 = 0.032; P = 0.011) and plasma MIP-1ß level (R2 = 0.027; P = 0.044). Random forest identified that the families Prevotellaceae, Moraxellaceae, and Staphylococcaceae were the biomarkers related to the positive bacteria lab test results. Multivariable Cox regression showed that the increase in α-diversity and the abundance of the families Prevotellaceae and Actinomycetaceae were associated with clinical improvements. The positive bacteria lab test results, AKI, and plasma MIP-1ß level were associated with patients' lung microbiota composition on ICU admission. The families Prevotellaceae and Actinomycetaceae on admission predicted clinical improvements.

High ratio of measles-specific IgG/IgM associated with nodular pneumonia in vaccinated individuals.

BACKGROUND: Vaccinated individuals infected with measles can develop nodular pneumonia. These cases can be misdiagnosed due to the absence of specific IgM and typical symptoms. An effective diagnostic tool is needed. METHODS: During March 2016, adult inpatients in Yucheng People's Hospital were enrolled prospectively and included in the study. Patients were included if samples were obtained ≤14 days from the onset of fever. Measles virus was detected by RT-PCR of the oropharyngeal swab sample. Chest computed tomography scans and medical records were obtained. Oropharyngeal swabs and blood samples were collected for IgM and IgG testing, RT-PCR, and gene sequencing. RESULTS: Sixteen patients were enrolled. Ten were found to have nodular pneumonia and were defined as the nodular group. The remaining six patients were defined as the control group. Measles-specific IgG titers in the nodular group were high (3618.3-5000mIU/ml), while IgM titers were low (<25mIU/ml); IgG titers in the control group were low (241.4-2560.3mIU/ml), while IgM titers were high (137-5000mIU/ml). No obvious viral mutation was detected in the nodular group. CONCLUSIONS: Measles-associated nodular pneumonia was only evident in those patients with an IgG/IgM ratio >20. In measles outbreaks, the IgG/IgM ratio may be useful to identify nodular pneumonia.