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1.
Small ; : e2311755, 2024 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-38676347

RESUMO

Numerous defects exist at the buried interface between the perovskite and adjacent electron transport layers in perovskite solar cells, resulting in severe non-radiative recombination and excessive open-circuit voltage (VOC) loss. Herein, a dual defect passivation strategy utilizing guanidine sulfate (GUA2SO4) as an interface modifier is first reported. On the one hand, the SO4 2- preferentially interacts with Pb-related defects, generating water-insoluble lead oxysalts complexes. Additionally, GUA+ diffuses into the perovskite and induces the formation of low-dimensional perovskite. These reactions effectively suppress trap states at the buried interface and perovskite boundaries in printable mesoscopic perovskite solar cells (p-MPSCs), thus increasing the carrier lifetime. Meanwhile, GUA2SO4 optimizes the interface energy band alignment, thus accelerating the charge extraction and transfer at the buried interface. This synergistic effect of trap passivation and interface energy band alignment modulation is strongly demonstrated by an increase in average VOC of 70 mV and the power conversion efficiency improvement from 17.51% to 18.70%. This work provides a novel approach to efficiently improve the performance of p-MPSCs through dual-targeted defect passivation at the buried interface.

2.
Small ; 19(47): e2303399, 2023 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-37505478

RESUMO

In this study, using PM6:L8-BO as the main system and non-fullerene acceptor IDIC as the third component, a series of ternary organic solar cells (TOSCs) are fabricated. The results reveal that IDIC plays a significant role in enhancing the performance of TOSCs by optimizing the morphology of blended films and forming interpenetrating nanostructure. The improved film morphology facilitates exciton dissociation and collection in TOSCs, which causes an increase in the short-circuit current density (JSC ) and fill factor (FF). Further, by optimizing the IDIC content, the power conversion efficiency (PCE) of TOSCs reaches 18.9%. Besides, the prepared TOSCs exhibit a JSC of 27.51 mA cm-2 and FF of 76.64%, which are much higher than those of PM6:L8-BO-based organic solar cells (OSCs). Furthermore, the addition of IDIC improves the long-term stability of the OSCs. Meanwhile, TOSCs with a large effective area of 1.00 cm2 have been prepared, which exhibit a PCE of 12.4%. These findings suggest that modifying the amount of the third component can be a useful strategy to construct hight-efficiency TOSCs with practical application potential.

3.
Microb Pathog ; 183: 106291, 2023 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-37557932

RESUMO

Enterobacter cloacae is a member of the Enterobacter family, which could prevent Macrobrachium rosenbergii from growing and cause mass mortality. However, no research has focused on microRNA immunity in M. rosenbergii infected with E. cloacae. To clarify the immune response mechanisms, transcriptomic analysis was performed on the miRNAs of M. rosenbergii infected with E. cloacae YZ3 strain. Following quality screening, 10,616,712 clean reads were obtained from the control group and 12,726,421 from the infected group. Among 899 known miRNAs, 446 differentially expressed miRNAs (DEMs) were identified. Meanwhile, 59 novel miRNAs were predicted, along with 39 DEMs. Target genes of DEMs have been predicted in order to gain a deeper understanding of the immune-related functions. GO and KEGG pathway analysis revealed the biological functions and signaling pathways of target genes. The results indicated that E. cloacae significantly affected the NOD-like receptor, RIG-I-like receptor and Toll-like receptor pathways. Ten DEMs were randomly selected, and their expression level was verified by Quantitative Real-time PCR technology. Overall, this study highlights the influential role of miRNAs in the innate immune system of M. rosenbergii, which has important implications for developing new strategies to prevent and treat related diseases in the future.


Assuntos
MicroRNAs , Palaemonidae , Animais , Transcriptoma , Palaemonidae/genética , Enterobacter cloacae/genética , Perfilação da Expressão Gênica , Imunidade , MicroRNAs/genética , MicroRNAs/metabolismo
4.
Microb Pathog ; 174: 105921, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36470347

RESUMO

Red sea bream iridovirus (RSIV) belongs to the family Iridoviridae, genus Megalocytivirus, which could widely infect marine fish, causing diseases and huge economic losses. Now it has been reported that RSIV was also detected in diseased mandarin fish. Transmission electron microscopy and immunohistochemistry showed that spleen was the main target organ in mandarin fish infected with RSIV. To investigate the immune response mechanism of mandarin fish to RSIV infection, transcriptomics of RSIV-infected mandarin fish was analyzed. A total of 53,040 unigenes were obtained, and there were 21,576 and 17,904 unigenes had significant hit the Nr and SwissProt databases, respectively. In RSIV-infected and non-infected spleen tissues, there were 309 differentially expressed genes (DEGs), including 100 up-regulated genes and 209 down-regulated genes. Gene Ontology database (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analysis were performed to reveal the function information and give a better understanding of the signal transduction pathways of DEGs. Further analysis of the cytokine-cytokine receptor interactions pathway exhibited that the expression of cytokines was widely activated after viral infection. In addition, ten DEGs were randomly selected and verified by quantitative real-time PCR, which revealed a similar expression tendency as the high-throughput sequencing data. These findings present valuable information that will benefit for better understanding of RSIV infection in mandarin fish.


Assuntos
Infecções por Vírus de DNA , Doenças dos Peixes , Iridoviridae , Iridovirus , Dourada , Viroses , Animais , Iridovirus/genética , Transcriptoma , Iridoviridae/genética , Infecções por Vírus de DNA/veterinária
5.
Fish Shellfish Immunol ; 135: 108692, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36924912

RESUMO

Non-O1 Vibrio cholerae, a member of the Vibrio family, could cause gastrointestinal infection of Macrobrachium rosenbergii and result in significant economic losses. However, few studies on microRNA immunity related to non-O1 V. cholerae infection of M. rosenbergii. The aim of this study was to elucidate the mechanism of miRNA in the potential immune response of M. rosenbergii. to non-O1 V. cholerae MSVC-GY01 infection by transcriptome sequencing. Following quality screening, the control group received 10, 616, 712 clean reads, whereas the infected group received 9,727,616. The miRNA sequences in the two samples are extremely consistent and have a length of roughly 23 nt. In all, 871 known miRNAs were discovered, with 279 differentially expressed miRNAs (DEMs). Meanwhile, 62 novel miRNAs were predicted, including 43 DEMs. In order to understand the immune-related biological functions of DEMs, target genes were predicted. Pathway function annotation analysis showed that non-O1 V. cholerae affected the NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, and Toll-like receptor signaling pathway, suggesting that miRNAs in the hepatopancreas play a key role in immune responses to pattern recognition receptors. Twelve DEMs were randomly selected for Quantitative Real-time PCR (qRT-PCR). Overall, the expression trends of qRT-PCR were consistent with the sequencing results. These findings corroborate the immunomodulatory function of miRNA in M. rosenbergii against non-O1 V. cholerae infection and provide guidance for the prevention and treatment of related illnesses.


Assuntos
Cólera , MicroRNAs , Palaemonidae , Vibrio cholerae não O1 , Animais , Transcriptoma , MicroRNAs/genética , MicroRNAs/metabolismo
6.
Fish Shellfish Immunol ; 135: 108641, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36858328

RESUMO

The largemouth bass virus (LMBV) isolate of Santee-Cooper ranavirus showed evidence of widespread infection in adult fish, but disease presentation caused by different viral strains exhibited considerable difference. In this study, a highly pathogenic LMBV-like resembling Santee-Cooper ranavirus was isolated and identified from juvenile largemouth bass. The pathogenicity and dynamic distribution of LMBV-like strain, histopathological analysis and host immune response of juvenile largemouth bass infected with LMBV-like were investigated. The results show that LMBV-like was highly pathogenic to juvenile fish, and the infected fish showed typical signs of acute haemorrhages and visceral enlargement. LMBV-like positive cells were found in the liver, spleen, kidney, gills, and intestinal tissue, and the virus content in spleen was the highest. Histopathological analysis showed different pathological changes in major tissues of diseased fish, mostly manifested as infiltration of inflammatory cell and histiocyte necrosis. In addition, humoral immune factors such as superoxide dismutase (SOD), catalase (CAT) and acid phosphatase (ACP) were used as serum indicators to evaluate the immune response of juvenile fish after infection. Quantitative real-time PCR (qRT-PCR) was used to evaluate the expression patterns of immune-related genes (CD40, IFN-γ, IgM, IL-1ß, IL-8, IL-12a, Mxd3, TGF-ß, and TNFα) in liver, spleen, and head kidney tissues. The results showed that immunological activity of the juvenile largemouth bass was significantly enhanced by LMBV-like infection. This research comprehensively systematically revealed the pathogenic characteristics of LMBV-like separated from juvenile largemouth bass and properties of the host's immune response caused by the virus infection, which providing a basis for further exploring the interaction between the virus and the host, and prevention and treatment of disease caused by Santee-Cooper ranavirus.


Assuntos
Bass , Infecções por Vírus de DNA , Doenças dos Peixes , Ranavirus , Animais , Virulência , Infecções por Vírus de DNA/veterinária
7.
ACS Appl Mater Interfaces ; 16(8): 10170-10179, 2024 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-38359458

RESUMO

Low bandgap organic semiconductors have been widely employed to broaden the light response range to utilize much more photons in the inverted perovskite solar cells (PSCs). However, the serious charge recombination at the heterointerface contact between perovskite and organic semiconductors usually leads to large energy loss and limits the device performance. In this work, a titanium chelate, bis(2,4-pentanedionato) titanium(IV) oxide (C10H14O5Ti), was directly used as an interlayer between the perovskite layer and organic bulk heterojunction layer for the first time. Impressively, it was found that C10H14O5Ti can not only increase the surface potential of perovskite films but also show a positive passivation effect toward the perovskite film surface. Drawing from the above function, a smoother perovskite active layer with a higher work function was realized upon the use of C10H14O5Ti. As a result, the C10H14O5Ti-modified integrated devices show lower interfacial loss and obtain the best power conversion efficiency (PCE) of up to 20.91% with a high voltage of 1.15 V. The research offers a promising strategy to minimize the interfacial loss for the preparation of high-performance perovskite solar cells.

8.
ChemSusChem ; : e202301741, 2024 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-38511510

RESUMO

The large open circuit voltage (VOC) loss is currently one of the main obstacles to achieving efficient organic solar cells (OSCs). In this study, the ternary OSCs comprising PM6:BTP-eC9:IT-4F demonstrate a superior efficiency of 18.2 %. Notably, the utilization of the medium bandgap acceptor IT-4F as the third component results in an exceptionally low nonradiative recombination energy loss of 0.28 V. The desirable energy level cascade is formed among PM6, BTP-eC9, and IT-4F due to the low-lying HOMO and LUMO energy levels of IT-4F. More importantly, the VOC of PM6:BTP-eC9:IT-4F OSCs can reach as high as 0.86 V, which is higher than both binary OSCs without sacrificing JSC and FF. Besides, this strategy proved that IT-4F can not only broaden the absorption range but also work as a morphology modifier in PM6:BTP-eC9:IT-4F OSCs, and there also exists efficient energy transfer between BTP-eC9 and IT-4F. This result provides a promising way to suppress the nonradiative recombination energy loss and realize higher VOC than the two binary OSCs in ternary OSCs to obtain high power conversion efficiencies.

9.
ACS Appl Mater Interfaces ; 16(6): 7265-7274, 2024 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-38318768

RESUMO

Carbon-based printable mesoscopic perovskite solar cells (MPSCs) have promising commercial development due to the use of easily scalable printing processes and low-cost carbon material electrodes. Simplifying the preparation process of MPSCs will undoubtedly contribute to their practical application. Here, we demonstrate that efficient and stable MPSCs can be prepared at room temperature without annealing by using low boiling point 2-methoxyethanol (2-ME) and strongly coordinated N-methyl-2-pyrrolidone (NMP) as a novel mixed solvent under the synergistic effect of ammonium chloride (NH4Cl). The results show that the 2-ME/NMP mixed solvent can generate an optimized coordination environment so that uniform nucleation and crystallization of perovskites in mesopores can be achieved at room temperature without annealing by forming uniform small-sized colloids in the precursor solution. Moreover, our work for the first time introduces NH4Cl as a crystallization modulator during a room-temperature annealing-free process, effectively regulating the crystallization behavior of perovskite in mesopores and obtaining high-quality perovskites. Finally, MPSCs prepared synergistically by a room-temperature annealing-free process based on a low boiling point 2-ME/NMP mixed solvent and NH4Cl modulator achieved a champion power conversion efficiency of 17.34% while demonstrating excellent long-term air stability for over half a year. This work provides a new approach to simplifying the preparation process of MPSCs and preparing efficient and stable MPSCs through a room-temperature annealing-free process.

10.
ACS Appl Mater Interfaces ; 15(21): 25774-25782, 2023 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-37193670

RESUMO

Additive strategies play a critical role in improving the performance of organic solar cells (OSCs). There are only a few reports on the application of solid additives for OSCs, which leaves a large space for further improvement of solid additives and further study on the relationship between material structure and property. PM6:BTP-eC9-based organic solar cells (OSCs) were prepared by using a small molecule BTA3 as a solid additive, and a high energy conversion efficiency of 18.65% is achieved. BTA3 has good compatibility with the acceptor component (BTP-eC9) and optimizes the morphology of the thin films. Moreover, the introduction of a small amount of BTA3 (5 wt %) effectively promotes exciton dissociation and charge transfer and suppresses charge recombination, and the relationship between the BTA3 content and the device parameter is deeply revealed. The use of BTA3 in the active layers is an attractive and effective strategy for high-performance OSCs.

11.
ACS Appl Mater Interfaces ; 14(7): 9161-9171, 2022 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-35142503

RESUMO

Carbon-based mesoscopic perovskite solar cells (MPSCs) are becoming one of the most competitive photovoltaic technologies owing to their lower manufacturing cost and excellent stability. In this work, methylammonium acetate (MAAc), an ionic liquid additive, is added into methylammonium lead triiodide (MAPbI3) perovskite and is used to fabricate high-performance MPSCs. Systematic and detailed studies have shown that the MAAc interacts with PbI2 preferentially to form a MAPbI3-x(Ac)x intermediate phase that can effectively control the crystallization kinetics of MAPbI3 in the triple-mesoscopic layer. MAPbI3 films with an appropriate amount of MAAc exhibit higher crystallinity, lower defect density, and dense pore filling, which effectively reduce carrier non-radiative recombination loss in MPSCs. As a result, a champion power conversion efficiency (PCE) of 13.54% is obtained based on the optimized MAAc-engineered MPSCs. The PCE is 24% higher than 10.90% of the control devices. Moreover, unencapsulated MAAc-engineered MPSCs retain 90% of their initial PCE after being stored in the dark for 50 days under ambient atmosphere, which demonstrates much better air stability than control devices. This work provides an effective strategy for developing efficient and stable carbon-based MPSCs with an eco-friendly ionic liquid additive.

12.
ACS Appl Mater Interfaces ; 13(38): 45435-45445, 2021 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-34542284

RESUMO

The emerging carbon-based mesoscopic perovskite solar cells (MPSCs) are known as one of the most promising candidates for photovoltaic applications thanks to their screen-printing process and excellent stability. Unfortunately, they usually suffer from serious defects because it is challenging to realize sufficient mesopore filling of the perovskite precursor solution throughout the triple-mesoporous scaffold. Herein, a bifunctional additive, biuret, endowed with both carbonyl and amino groups, was designed to realize a convenient fabrication approach for controllable crystallization of the precursor solution. Owing to the strong coordination ability with perovskite components, the incorporation of biuret can not only regulate crystallization kinetics allowing for the growth of high-quality perovskite crystals but also associate with uncoordinated ions for defect passivation to enhance the overall photovoltaic performance of MPSCs. A champion power conversion efficiency (PCE) of 13.42% with an enhanced short-circuit current density of 19.49 mA cm-2 and a much higher open-circuit voltage of 0.96 V was achieved for the device doped with 3 mol % biuret, which is 26% higher than that of the control device (10.66%). Moreover, the unencapsulated devices with biuret incorporation demonstrated superior stability, maintaining over 90% of the original PCE after 50 days of storage under ambient conditions. This work helps exploit bifunctional additive strategies for simultaneous defect passivation and crystallization control toward high-efficiency and long-term stability of carbon-based MPSCs.

13.
PLoS One ; 14(7): e0220005, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31329618

RESUMO

In present work, a highly sensitive biosensor with high selectivity for glucose monitoring is developed based on novel nano-composites of nitrogen doped graphene quantum dots (N-GQDs) and a novel bimetallic Cu/Ni core-shell nanoparticles (CSNPs) (Cu@Ni CSNPs/N-GQDs NCs). With the tuned electronic properties, N-GQDs helped bimetallic core-shell structure nanomaterials from aggregation, and separate the charges generated at the interface. This novel nano-composites also have the good electrical conductivity of N-GQDs, catalyst property of Cu/Ni bimetallic nano composite, Cu@Ni core-shell structure and the synergistic effect of the interaction between bimetallic nano composite and N-GQDs. While modified the electrode with this novel nano-composites, the sensor' linear range is 0.09 ~ 1 mM, and the limit of detection (LOD) is 1.5 µM (S/N = 3) with a high sensitivity of 660 µA mM-1 cm-2, and rapid response time (3 s). Its' LOD is more than 74 times lower than the traditional Cu@Ni CSNPs modified working electrode. It also has higher sensitivity and wider linear range. This indicates the great potential of applying this kind of nano composites in electrode modification.


Assuntos
Glucose/análise , Grafite/química , Nanopartículas Metálicas/química , Nanocompostos/química , Técnicas Biossensoriais/métodos , Técnicas Biossensoriais/normas , Cobre/química , Níquel/química , Nitrogênio/química , Sensibilidade e Especificidade
14.
Biochim Biophys Acta ; 1556(2-3): 273-7, 2002 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-12460686

RESUMO

We synthesized a kind of gold nanoparticle protected by a synthetic lipid (didodecyldimethylammonium bromide, DDAB). With the help of these gold nanoparticles, hemoglobin can exhibit a direct electron transfer (DET) reaction. The formal potential locates at -169 mV vs. Ag/AgCl. Spectral data indicated the hemoglobin on the electrode was not denatured. The lipid-protected gold nanoparticles were very stable (for at least 8 months). Their average diameter is 6.42 nm. It is the first time to use monolayer-protected nanoparticles to realize the direct electrochemistry of protein.


Assuntos
Carbono/química , Eletrodos , Transporte de Elétrons , Ouro/química , Hemoglobinas/química , Lipídeos/química , Eletroquímica , Hemina/química , Lipídeos/síntese química , Tamanho da Partícula , Análise Espectral/métodos
15.
Artigo em Inglês | MEDLINE | ID: mdl-15683801

RESUMO

Electrochemical redox processes of bovine heart cytochrome c were investigated by in situ UV-vis and CD spectroelectrochemistry at bare glassy carbon electrode (GCE) and single-wall carbon nanotubes (SWNTs) modified glassy carbon electrode (SWNTs/GCE) using a long optical path thin layer cell. The spectra obtained at GCE and SWNTs/GCE reflect electrode surface microstructure-dependent changes in protein conformation during redox transition. Potential-dependent conformational distribution curves of cytochrome c obtained by analysis of in situ circular dichroism (CD) spectra using singular value decomposition least square (SVDLS) method show that SWNTs can retain conformation of cytochrome c. Some parameters of the electrochemical reduction process, i.e. the product of electron transfer coefficient and number of electrons (alpha n = 0.3), apparent formal potential (E0' = 0.04 V), were obtained by double logarithmic analysis and standard heterogeneous electron transfer rate constant k0 was obtained by electrochemistry and double logarithmic analysis, respectively.


Assuntos
Citocromos c/química , Animais , Bovinos , Dicroísmo Circular , Eletroquímica , Eletrodos , Análise dos Mínimos Quadrados , Nanotubos de Carbono , Conformação Proteica , Espectrofotometria Ultravioleta
16.
Biosens Bioelectron ; 18(10): 1225-30, 2003 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-12835040

RESUMO

A highly catalytic activity microperoxidase-11 (MP-11) biosensor for H(2)O(2) was developed to immobilizing the heme peptide in didodecyldimethylammonium bromide (DDAB) lipid membrane. The enzyme electrode thus obtained responded to H(2)O(2) without electron mediator or promoter, at a potential of +0.10 V versus Agmid R:AgCl. A linear calibration curve is obtained over the range from 2.0 x 10(-5) to 2.4 x 10(-3) M. The biosensor responds to hydrogen peroxide in 15 s and has a detection limit of 8 x 10(-7) M (S/N=3) Providing a natural environment with lipid membrane for protein immobilization and maintenance of protein functions is a suitable option for the design of biosensors.


Assuntos
Peróxido de Hidrogênio/análise , Membranas Artificiais , Peroxidases/metabolismo , Compostos de Amônio Quaternário/metabolismo , Técnicas Biossensoriais , Eletrodos , Concentração de Íons de Hidrogênio , Lipossomos/química , Lipossomos/metabolismo , Peroxidases/química , Compostos de Amônio Quaternário/química , Espectroscopia de Infravermelho com Transformada de Fourier , Fatores de Tempo
17.
Chem Commun (Camb) ; (14): 1540-1, 2002 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-12189883

RESUMO

A novel approach of generating cathodic electrochemiluminescence of Ru(bpy)3(2+) at -0.4 V triggered by reactive oxygen species is reported for detecting alkylamines and some organic acids.

18.
Biophys Chem ; 97(1): 7-16, 2002 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-12052491

RESUMO

The conformational transition of DNA induced by the interaction between DNA and a cationic lipid vesicle, didodecyldimethylammonium bromide (DDAB), had been investigated by circular dichroism (CD) and UV spectroscopy methods. We used singular value decomposition least squares method (SVDLS) to analyze the experimental CD spectra. Although pH value influenced the conformation of DNA in solution, the results showed that upon binding to double helical DNA, positively charged liposomes induced a conformational transition of DNA molecules from the native B-form to more compact conformations. At the same time, no obvious conformational changes occurred at single-strand DNA (ssDNA). While the cationic lipid vesicles and double-strand DNA (dsDNA) were mixed at a high molar ratio of DDAB vesicles to dsDNA, the conformation of dsDNA transformed from the B-form to the C-form resulting in an increase in duplex stability (DeltaT(m)=8+/-0.4 degrees C). An increasing in T(m) was also observed while the cationic lipid vesicles interacted with ssDNA.


Assuntos
Cátions/química , DNA de Cadeia Simples/química , DNA/química , Lipídeos/química , Dicroísmo Circular , Conformação de Ácido Nucleico , Desnaturação de Ácido Nucleico , Compostos de Amônio Quaternário/química , Espectrofotometria Ultravioleta
19.
Biophys Chem ; 110(3): 203-11, 2004 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-15228956

RESUMO

pH-dependent processes of bovine heart ferricytochrome c have been investigated by electronic absorption and circular dichroism (CD) spectra at functionalized single-wall carbon nanotubes (SWNTs) modified glass carbon electrode (SWNTs/GCE) using a long optical path thin layer cell. These methods enabled the pH-dependent conformational changes arising from the heme structure change to be monitored. The spectra obtained at functionalized SWNTs/GCE reflect electrode surface microstructure-dependent changes for pH-induced protein conformation, pK(a) of alkaline transition and structural microenvironment of the ferricytochrome c heme. pH-dependent conformational distribution curves of ferricytochrome c obtained by analysis of in situ CD spectra using singular value decomposition least square (SVDLS) method show that the functionalized SWNTs can retain native conformational stability of ferricytochrome c during alkaline transition.


Assuntos
Citocromos c/química , Citocromos c/metabolismo , Ferro/metabolismo , Animais , Bovinos , Dicroísmo Circular , Eletrodos , Concentração de Íons de Hidrogênio , Estrutura Terciária de Proteína , Análise Espectral
20.
Biophys Chem ; 99(3): 271-9, 2002 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-12408941

RESUMO

Nisin is a positively charged antibacterial peptide that binds to the negatively charged membranes of gram-positive bacteria. The initial interaction of the peptide with the model membrane of negatively charged DPPG (dipalmitoylphosphatidylglycerol) was studied by cyclic voltammetry and a.c. impedance spectroscopy. Nisin could induce pores in the supported bilayer lipid membrane, thus, it led to the marker ions Fe(CN)(6)(3-/4-) crossing the lipid membrane and giving the redox reaction on the glassy carbon electrode (GCE). Experimental results suggested that the pore formation on supported bilayer lipid membrane was dependent on the concentration of nisin and it included three main concentration stages: low, middling, high concentration.


Assuntos
Antibacterianos/química , Bicamadas Lipídicas/química , Nisina/química , Algoritmos , Sequência de Aminoácidos , Detergentes/química , Impedância Elétrica , Eletroquímica , Indicadores e Reagentes , Membranas Artificiais , Dados de Sequência Molecular , Fosfatidilgliceróis/química , Porosidade
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