A Dynamic Multi-Scale Convolution Model for Face Recognition Using Event-Related Potentials.

With the development of data mining technology, the analysis of event-related potential (ERP) data has evolved from statistical analysis of time-domain features to data-driven techniques based on supervised and unsupervised learning. However, there are still many challenges in understanding the relationship between ERP components and the representation of familiar and unfamiliar faces. To address this, this paper proposes a model based on Dynamic Multi-Scale Convolution for group recognition of familiar and unfamiliar faces. This approach uses generated weight masks for cross-subject familiar/unfamiliar face recognition using a multi-scale model. The model employs a variable-length filter generator to dynamically determine the optimal filter length for time-series samples, thereby capturing features at different time scales. Comparative experiments are conducted to evaluate the model's performance against SOTA models. The results demonstrate that our model achieves impressive outcomes, with a balanced accuracy rate of 93.20% and an F1 score of 88.54%, outperforming the methods used for comparison. The ERP data extracted from different time regions in the model can also provide data-driven technical support for research based on the representation of different ERP components.

Retracted: Role of Paeonol in an Astrocyte Model of Parkinson Disease.

The authors wish to retract the article. In this article, they found that astrocytes that were pretreated with paeonol significantly rescued MPP+-induced cell viability reduction, and inhibited up-regulation of cell apoptosis, caspase-1 activity, COX2, iNOS, and Bax/Bcl-2 ratio, as well as p-JNK and p-ERK. These findings suggest that paeonol is a neuroprotective agent suitable for use in treatment of PD. However, in subsequent research, they examined the protein levels of p-JNK/p-ERK/p-P38 in different groups. Results showed that in the MPP+ groups, not all these protein levels were higher than those in the control group, because of the flawed data presentations. They also used western blot analysis to assess protein levels of Bax and Bcl-2 in astrocytes. Compared with the control group, Bax protein level was increased, while Bcl-2 protein level was decreased after treatment with MPP+, and these changes were not reversed by paeonol. Based on the above, they ascertained that there must have been some serious mistake in their experiment. As a result, all authors agreed to retract this article. Reference: Maosheng Ye, Yuxin Yi, Shixing Wu, Yong Zhou, Dongji. Role of Paeonol in an Astrocyte Model of Parkinson Disease. Med Sci Monit, 2017; 23: 4740-4748. DOI: 10.12659/MSM.906716.

Role of Paeonol in an Astrocyte Model of Parkinson's Disease.

BACKGROUND Parkinson's disease (PD) is characterized by a progressive degeneration of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNc). Inflammation and neural degeneration are implicated in the pathogenesis of PD. Paeonol has been verified to attenuate inflammation. MATERIAL AND METHODS 1-methyl-4-phenylpyridnium ion (MPP+, 100 µM) was used to induce the cell model of PD in primary cultured astrocytes. Astrocyte cell viability and apoptosis were determined by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay and flow cytometry (FCM), respectively. Protein levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthases (iNOS) in culture medium were tested by enzyme-linked immunosorbent (ELISA) assay. Protein levels of casapse-1, COX2, iNOS, B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax), Bcl-2, and phosphorylated Jun N-terminal kinase (p-JNK)/phosphorylated extracellular signal-regulated kinase (p-ERK)/p-P38 were examined by Western blot. RESULTS Pretreatment with paeonol remarkably rescued MPP+-induced cell viability reduction, up-regulation of cell apoptosis, caspase-1 activity, COX-2, iNOS, and Bax/Bcl-2 ratio in primary astrocytes. Furthermore, paeonol repressed MPP+ -induced elevation of p-JNK/p-ERK in primary cultured astrocytes. CONCLUSIONS The present study found that paeonol protected cells from apoptosis by repressing the activation of the JNK/ERK related signalling pathway induced by MPP+ in astrocytes. We propose that paeonol is a neuroprotective agent for the treatment of PD patients, with great promise in the future.

Nondestructive In Situ Measurement Method for Kernel Moisture Content in Corn Ear.

Moisture content is an important factor in corn breeding and cultivation. A corn breed with low moisture at harvest is beneficial for mechanical operations, reduces drying and storage costs after harvesting and, thus, reduces energy consumption. Nondestructive measurement of kernel moisture in an intact corn ear allows us to select corn varieties with seeds that have high dehydration speeds in the mature period. We designed a sensor using a ring electrode pair for nondestructive measurement of the kernel moisture in a corn ear based on a high-frequency detection circuit. Through experiments using the effective scope of the electrodes' electric field, we confirmed that the moisture in the corn cob has little effect on corn kernel moisture measurement. Before the sensor was applied in practice, we investigated temperature and conductivity effects on the output impedance. Results showed that the temperature was linearly related to the output impedance (both real and imaginary parts) of the measurement electrodes and the detection circuit's output voltage. However, the conductivity has a non-monotonic dependence on the output impedance (both real and imaginary parts) of the measurement electrodes and the output voltage of the high-frequency detection circuit. Therefore, we reduced the effect of conductivity on the measurement results through measurement frequency selection. Corn moisture measurement results showed a quadric regression between corn ear moisture and the imaginary part of the output impedance, and there is also a quadric regression between corn kernel moisture and the high-frequency detection circuit output voltage at 100 MHz. In this study, two corn breeds were measured using our sensor and gave R² values for the quadric regression equation of 0.7853 and 0.8496.

Perceptually Inspired C0-Continuity Haptic Shape Display with Trichamber Soft Actuators.

Shape display devices composed of actuation pixels enable dynamic rendering of surface morphological features, which have important roles in virtual reality and metaverse applications. The traditional pin-array solution produces sidestep-like structures between neighboring pins and normally relies on high-density pins to obtain curved surfaces. It remains a challenge to achieve continuous curved surfaces using a small number of actuated units. To address the challenge, we resort to the concept of surface continuity in computational geometry and develop a C0-continuity shape display device with trichamber fiber-reinforced soft actuators. Each trichamber unit produces three-dimensional (3D) deformation consisting of elongation, pitch, and yaw rotation, thus ensuring rendered surface continuity using low-resolution actuation units. Inspired by human tactile discrimination threshold on height and angle gradients between adjacent units, we proposed the mathematical criteria of C0-continuity shape display and compared the maximal number of distinguishable shapes using the proposed device in comparison with typical pin-array. We then established a shape control model considering the nonlinearity of soft materials to characterize and control the soft device to display C0-continuity shapes. Experimental results showed that the proposed device with nine trichamber units could render typical sets of distinguishable C0-continuity shape sequence changes. We envision that the concept of C0-continuity shape display with 3D deformation capability could improve the fidelity of the rendered shapes in many metaverse scenarios such as touching human organs in medical palpation simulations.

Temperature-Induced Structure Transformation from Co0.85Se to Orthorhombic Phase CoSe2 Realizing Enhanced Hydrogen Evolution Catalysis.

Transition-metal chalcogenides (TMC) have been widely studied as active electrocatalysts toward the hydrogen evolution reaction due to their suitable d-electron configuration and relatively high electrical conductivity. Herein, we develop a feasible method to synthesize an orthorhombic phase of CoSe2 (o-CoSe2) from the regeneration of Co0.85Se, where the temperature plays a key role in controlling the structure transformation. To the best of our knowledge, this is the first report about this synthetic route for o-CoSe2. The resulting o-CoSe2 catalysts exhibit enhanced hydrogen evolution reaction performance with an overpotential of 220 mV to reach 10 mA cm-2 in 1.0 M KOH. Density functional theory calculations further reveal that the change in the Gibbs free energy of hydrogen, water adsorption energy, and the downshifted d-band center make o-CoSe2 more suitable for accelerating the HER process.

The relationship between TEM8 and early diagnosis and prognosis of lung cancer.

BACKGROUND: To explore the TEM8 expression in patients with lung cancer and its relationship with clinical pathology and prognosis, and to analyze the diagnostic value of TEM8. METHODS: A total of 204 patients with lung cancer diagnosed and treated in Zhongmeng Hospital Zhalantun and the First Affiliated Hospital of Jinzhou Medical University from March 2013 to February 2016 were enrolled in the study group, and 203 healthy subjects in the control group. qRT-PCR technique was applied to detect the TEM8 expression. Combined with clinical information, the diagnostic value of TEM8 for lung cancer and the correlation of clinical characteristics of TEM8 were analyzed. The 3-year survival curves of patients with low and high TEM8 expressions were compared. RESULTS: The expression in the study group was significantly higher than that in the control group (P<0.05). When the cut-off value was 1.125, the sensitivity, specificity and AUC of TEM8 in the diagnosis of lung cancer were 50.00%, 98.00% and 0.726 respectively. The TEM8 expression also differs when in smoking, lymphatic metastasis, TNM stage, differentiation degree and pleural invasion classification (P<0.050). 132 patients were included in the survival group and 72 patients were included in the death group. There was a difference between the two groups in the effect of TEM8 on the prognosis (P<0.001). CONCLUSIONS: TEM8 showed high expression in the study group. TEM8 had good diagnostic efficacy and was expected to be an excellent indicator for early clinical diagnosis and prognosis of lung cancer.

Plant electrical signals: A multidisciplinary challenge.

Plant electrical signals, an early event in the plant-stimulus interaction, rapidly transmit information generated by the stimulus to other organs, and even the whole plant, to promote the corresponding response and trigger a regulatory cascade. In recent years, many promising state-of-the-art technologies applicable to study plant electrophysiology have emerged. Research focused on expression of genes associated with electrical signals has also proliferated. We propose that it is appropriate for plant electrical signals to be considered in the form of a "plant electrophysiological phenotype". This review synthesizes research on plant electrical signals from a novel, interdisciplinary perspective, which is needed to improve the efficient aggregation and use of plant electrical signal data and to expedite interpretation of plant electrical signals.

[Measurement of pork tenderness by using steady spatially-resolved spectroscopy].

Tenderness is one of the main sensory and eating qualities of meat. Conventional measurement of tenderness is a time-consuming and invasive method. Using steady spatially-resolved spectroscopy, a multi-channel visible and near infrared spectroscopy instrument was established to obtain the reduced scattering coefficient mu's. of porcine longissimus muscle samples. After spectra collection, each fresh meat sample was divided into two parts, one was tested by means of C-LM4 tenderness instrument, and the other was measured by conventional method. The results showed that reduced scattering coefficient of the samples was significantly correlated to fresh meat shear force values (R2 = 0.8349) at 700 nm. Also, there was a significant correlation between fresh meat shear force values and shear force values by conventional method (R2 = 0.7716). In conclusion, the potential of the steady spatially-resolved spectroscopy technique as a rapid and non-invasive tool to measure tenderness of pork was found.

Research on motion planning for an indoor spray arm based on an improved potential field method.

The target spraying effect of spray robots mainly depends on the control performance of the spraying arm during the processes of aiming and tracking. To further improve the robustness of the endpoint control and positioning accuracy of the spray arm, an improved potential field algorithm for the motion planning and control of the spray arm is proposed based on prophase research. The algorithm introduces a velocity potential field, visual field constraints and joint position limit constraints into the traditional artificial potential field method. The velocity potential field is used to ensure that the target state of the spraying arm is at the same velocity as the target crop (relative velocity) to achieve stable target tracking. The visual field constraints and joint position limit constraints are utilized to ensure the efficiency of the visual servo control and the movement of the spray arm. The algorithm can plan a feasible trajectory for the spraying arm in Cartesian space and image space, and use the speed controller to control the spraying arm movement along the trajectory for aiming and tracking. Simulation analysis shows that the algorithm can plan better motion trajectories than the servo controller based on image moments in previous studies. In addition, the experimental results show that the algorithm can effectively improve the robustness of targeting and tracking control for the spray robot.

A Resource for Inactivation of MicroRNAs Using Short Tandem Target Mimic Technology in Model and Crop Plants.

microRNAs (miRNAs) are endogenous small non-coding RNAs that bind to mRNAs and target them for cleavage and/or translational repression, leading to gene silencing. We previously developed short tandem target mimic (STTM) technology to deactivate endogenous miRNAs in Arabidopsis. Here, we created hundreds of STTMs that target both conserved and species-specific miRNAs in Arabidopsis, tomato, rice, and maize, providing a resource for the functional interrogation of miRNAs. We not only revealed the functions of several miRNAs in plant development, but also demonstrated that tissue-specific inactivation of a few miRNAs in rice leads to an increase in grain size without adversely affecting overall plant growth and development. RNA-seq and small RNA-seq analyses of STTM156/157 and STTM165/166 transgenic plants revealed the roles of these miRNAs in plant hormone biosynthesis and activation, secondary metabolism, and ion-channel activity-associated electrophysiology, demonstrating that STTM technology is an effective approach for studying miRNA functions. To facilitate the study and application of STTM transgenic plants and to provide a useful platform for storing and sharing of information about miRNA-regulated gene networks, we have established an online Genome Browser (https://blossom.ffr.mtu.edu/designindex2.php) to display the transcriptomic and miRNAomic changes in STTM-induced miRNA knockdown plants.

Wip1 regulates SKOV3 cell apoptosis through the p38 MAPK signaling pathway.

The aim of the present study was to explore the effect of silencing wild­type p53­induced phosphatase 1 (Wip1) on apoptosis of human ovarian cancer SKOV3 cells. SKOV3 cells cultured in vitro were divided into three groups: untreated cells, cells transfected with control small interfering RNA (siRNA) and cells transfected with siRNA targeting Wip1. Flow cytometry analysis was used to detect cell apoptosis. Western blot analysis was performed to determine expression of tumor protein 53 (p53), cleaved caspase­3, caspase­3, BCL2 associated X (Bax), BCL2 apoptosis regulator (Bcl­2), p38 mitogen­activated protein kinase (p38 MAPK) and phosphorylated (p)­p38 MAPK. Reverse transcription­quantitative polymerase chain reaction was used to detect expression of p53, Bax, Bcl­2 and caspase­3 mRNAs. Compared with control, apoptosis of SKOV3 cell was significantly increased following Wip1 siRNA silencing. Wip1 silencing also resulted in a significant increase of p53 and p­p38 MAPK expression, as well as increased cleaved caspase­3/caspase­3 and Bax/Bcl­2 protein ratios. No significant differences were observed in apoptosis and apoptosis­related protein expression in the control siRNA transfected cells. The present study demonstrated that Wip1 silencing promotes apoptosis of human ovarian cancer SKOV3 cells by activation of the p38 MAPK signaling pathways and through subsequent upregulation of p53, and cleaved caspase­3/caspase­3 and Bax/Bcl­2 protein ratios. Overall, the findings of the present study suggest that targeting Wip1 may be a potential therapeutic avenue for the treatment of human ovarian cancer in the future.

High-resolution non-contact measurement of the electrical activity of plants in situ using optical recording.

The limitations of conventional extracellular recording and intracellular recording make high-resolution multisite recording of plant bioelectrical activity in situ challenging. By combining a cooled charge-coupled device camera with a voltage-sensitive dye, we recorded the action potentials in the stem of Helianthus annuus and variation potentials at multiple sites simultaneously with high spatial resolution. The method of signal processing using coherence analysis was used to determine the synchronization of the selected signals. Our results provide direct visualization of the phloem, which is the distribution region of the electrical activities in the stem and leaf of H. annuus, and verify that the phloem is the main action potential transmission route in the stems of higher plants. Finally, the method of optical recording offers a unique opportunity to map the dynamic bioelectrical activity and provides an insight into the mechanisms of long-distance electrical signal transmission in higher plants.

Spatio-temporal mapping of variation potentials in leaves of Helianthus annuus L. seedlings in situ using multi-electrode array.

Damaging thermal stimuli trigger long-lasting variation potentials (VPs) in higher plants. Owing to limitations in conventional plant electrophysiological recording techniques, recorded signals are composed of signals originating from all of the cells that are connected to an electrode. This limitation does not enable detailed spatio-temporal distributions of transmission and electrical activities in plants to be visualised. Multi-electrode array (MEA) enables the recording and imaging of dynamic spatio-temporal electrical activities in higher plants. Here, we used an 8 × 8 MEA with a polar distance of 450 µm to measure electrical activities from numerous cells simultaneously. The mapping of the data that were recorded from the MEA revealed the transfer mode of the thermally induced VPs in the leaves of Helianthus annuus L. seedlings in situ. These results suggest that MEA can enable recordings with high spatio-temporal resolution that facilitate the determination of the bioelectrical response mode of higher plants under stress.