miRNA-23a has effects to improve lung injury induced by sepsis in vitro and vivo study.

AIM: The aim of this study is to explain the effects and mechanism of miRNA-23a in lung injury which were induced by sepsis in vitro and vivo. METHODS: In the vitro study, The BEAS-2B cells were divided into 4 groups: NC, MC, miRNA and miRNA + PTEN agonist groups. The cell proliferation and apoptosis of difference groups were measured by MTT and flow cytometry, the relative proteins expression of difference groups were measured by WB assay. In the vivo study, the rats were also divided into 4 groups: NC, MC, miRNA and miRNA + PTEN agonist groups. The miRNA-23a expression of difference groups were evaluated by ISH in lung tissues of rats. The cell apoptosis of difference groups were evaluated by TUNEL assay in lung tissues; the relative proteins expression of difference groups were evaluated by IHC assay. RESULTS: Compared with NC group, the cell apoptosis rate of MC groups were significantly increased in vitro and vivo studies (P ＜ 0.05, respectively). The relative proteins (PTEN, PI3K, AKT and P53) expressions of MC group were significantly differences (P ＜ 0.05, respectively) compared with those of NC groups in vitro and vivo studies. However, with miRNA-23a infection, the cell apoptosis of miRNA group were significantly suppressed compared with MC groups, and the relative proteins (PTEN, PI3K, AKT and P53) of miRNA group were also significantly differences compared with MC groups in vitro and vivo studies (P ＜ 0.05, respectively). CONCLUSION: The miRNA-23a has improved lung injury induced by sepsis via PTEN/PI3K/AKT/P53 pathway in vitro and vivo studies.

Exploring lipid markers of the quality of coix seeds with different geographical origins using supercritical fluid chromatography mass spectrometry and chemometrics.

BACKGROUND: Lipids, a group of primary metabolites, could be used as quality markers of Traditional Chinese medicine. PURPOSE: The present study was designed to develop a research method to explore lipid markers of the quality of coix seeds with different geographical origins. STUDY DESIGN: The geographical origins of coix seeds were divided into three regions based on the latitude. A central composite design (CCD test) was used to optimize the chromatographic parameters of supercritical fluid chromatography to obtain optimal lipid profile of coix seed. METHODS: An untargeted method based on ultra-performance convergence chromatography - quadrupole/time-of-flight hybrid mass spectrometry (UPC2-QTOF) was developed. Four chromatographic parameters were optimized using CCD test, and a fusion index established by Derringer function was used to evaluate. The lipid profile of 27 batches of coix seeds were acquired and processed by Progenesis QI software, and the MS/MS spectrums were obtained to identify, simultaneously. The difference lipids were explored by orthogonal partial least squares discriminant analysis (OPLS-DA). The lipids that showed differences depending on their seeds' geographical origin were selected as markers of the quality of coix seeds from the three regions. RESULTS: A Torus 2-PIC (1.7 µm, 100 mm × 3.0 mm) was selected as the optimal column of the untargeted method which the run time was only 8 minutes. From the CCD test, the interaction of chromatographic parameters between column temperature and backpressure was founded which the optimal parameters were 55 °C and 2600 psi, respectively. Thirty-two peaks in the lipid profile of coix seed were tentatively identified, of which 20 were triglyceride, and 12 were diglyceride. Nine features that could potentially be used to distinguish the coix seeds by their geographical origin were identified, most of which were diglycerides, such as OP. CONCLUSIONS: Our findings confirm that UPC2-QTOF combined with chemometrics could be used as an efficient method for exploring potential lipid markers of the quality of herbal medicine.