A chromosome-level genome assembly of the beet armyworm Spodoptera exigua.

BACKGROUND: The beet armyworm Spodoptera exigua is a polyphagous caterpillar that causes serious damage to many species of crops and vegetables. To gain insight into how this polyphagous insect differs from less harmful oligophagous species, we generated a chromosome-level assembly and compared it to closely related species with the same or different feeding habits. RESULTS: Based on Illumina and Pacific Biosciences data and Hi-C technology, 425.6 Mb of genome sequences were anchored and oriented into 31 linkage groups, with an N50 length of 14.8 Mb. A total of 24,649 gene models were predicted, of which 97.4% were identified in the genome assembly. Chemosensory genes are vital for locating food: of the four main families, odorant-binding proteins, chemosensory proteins and olfactory receptors showed little difference, whereas gustatory receptors are greatly expanded in S. exigua. Examination of other polyphagous insects confirmed this difference from oligophagous congeners and further identified the bitter receptor subfamily as being particularly affected. CONCLUSION: Our high-quality genome sequence for beet armyworm identified a key expansion of the bitter gustatory receptor subfamily in this and other pests that differs crucially from more benign relatives and offers insight into the biology and possible future means of control for these economically important insects.

The Expression of P35 Plays a Key Role in the Difference in Apoptosis Induced by AcMNPV Infection in Different Spodoptera exigua Cell Lines.

Baculovirus infection induces apoptosis in host cells, and apoptosis significantly affects virus production. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) can regulate apoptosis, but the regulatory mechanism is unclear. Here, we found that AcMNPV infection induced different apoptosis responses in different Spodoptera exigua cell lines. In the early stages of viral infection (1-6 h), Se-1 cells underwent severe apoptosis, while Se-3 cells underwent very slight apoptosis. In the late stages of viral infection (12-72 h), Se-1 cells continued to undergo apoptosis and formed a large number of apoptotic bodies, while the apoptosis of Se-3 cells was inhibited and no apoptotic bodies were formed. To determine the reasons for the apoptosis differences in the two cell lines, we measured the expression of the six S. exigua cysteine-dependent aspartate specific protease genes (SeCaspase-1 to -6) and the three AcMNPV antiapoptotic protein genes (iap1, iap2 and p35) during viral infection. We found that SeCaspase-1 to -6 were all activated in Se-1 cells and inhibited in Se-3 cells, whereas iap1, iap2 and p35 were all inhibited in Se-1 cells and normally expressed in Se-3 cells. And p35 was expressed earlier than iap1 and iap2 in Se-3 cells. Otherwise, Se-1 and Se-3 cells would all be apoptotic when infected with the recombinant p35 knockout AcMNPV, whereas only Se-1 cells were apoptotic, but Se-3 cells were not apoptotic when infected with the recombinant p35 repair AcMNPV. Combined with the fact that the expression of P35 protein is inhibited in Se-1 cells but normally expressed in Se-3 cells during the infection of recombinant p35 repair AcMNPV, we proposed that the different expression of P35 is an important reason for the apoptosis differences between the two cell lines. We also found that some genes associated with apoptosis can probably regulate the expression of P35. However, the major upstream regulators of P35 and their mechanisms are still unclear and will be studied in the future.

Performance and retention mechanisms of corn silk to atmospheric heavy metal lead.

Atmospheric heavy metals are seriously harmful, and porous materials have unique advantages in the control of air pollutants. However, the direct use of plant porous materials to purify atmospheric heavy metals are rare. So agricultural waste corn silk with porous structures was selected to analyze the retention capability and mechanism to the atmospheric lead. The results show that the corn silk can effectively retain atmospheric lead in natural growing state or fixed experimental conditions. The analysis of a total of 765 corn silk samples from 17 different regions in Shandong province, China, shows that atmospheric lead is the main source of lead in the corn silk, and corn silk can be used for biological monitoring of atmospheric lead to some extent. Based on the analysis with different techniques including scanning electron microscope (SEM), energy spectrum dispersive X-ray spectrometer (EDS), Fourier infrared spectrometer (FTIR) and Zeta potential, the effective retention of lead by corn silk is due to a variety of mechanisms, including physical adsorption, electrostatic adsorption, complexation, chelation and ion exchange. So agricultural waste corn silk has great potential in the application of biosorption or preparation of porous materials in purification of atmospheric heavy metals.

(1) Atmospheric lead is the main source of lead in the corn silk based on the analysis of a total of 765 corn silk samples from 17 different regions in Shandong province, China.(2) The pore diameter in the corn silk is 1.62­3.51 µm, suitable for lead retention, and the functional groups ­OH, ­COOH and ­O­ of the corn silk play a vital role in the lead retention process.(3) The potential value of agricultural waste corn silk was firstly revealed in the purification of atmospheric heavy metals.

Foliar uptake and transport of atmospheric trace metals bounded on particulate matters in epiphytic Tillandsia brachycaulos.

Epiphytic Tillandsia species are uniquely suitable for the study of foliar uptake of atmospheric trace metals (ATM) because these plants can only rely on their leaves for this purpose. Therefore, we analyzed the uptake and transport of different metals (Fe, Al, Zn, Mn, Ba, Ti, Cu, Ni, Cr, Sn, Pb, Co, As, and Se) bounded on atmospheric particulate matters (APM) in Tillandsia brachycaulos Schltdl. The results showed that the metal contents inside leaves significantly (p < .05) increased after APM exposure. There was a significant (p < .05) positive correlation between the content of 14 trace metals accumulated on the leaf surface and inside the leaf, which indicated that APM is the main source of ATM uptake. The subcellular analysis showed that the Pb, Cu, Ni, Zn, and Cr absorbed by T. brachycaulos were stored primarily in the cell walls and organelles. After the removal of foliar trichomes of T. brachycaulos, the metal contents on the leaf surface decreased, whereas the contents of most metals inside the leaf increased. This is an evidence that foliar trichomes serve a protective function by intercepting ATM onto the leaf surface.Novelty statementsThere was a significant positive correlation between the contents of 14 trace metals accumulated on the leaf surface and in the leaf of T. brachycaulos, which indicated that atmospheric particulate matters are the main source of trace metals in the leaves.After the removal of foliar trichomes of T. brachycaulos, the trace metal contents on the leaf surface decreased, whereas the contents of most trace metals inside the leaf increased. This is an evidence that foliar trichomes serve a protective function by intercepting atmospheric trace metals onto the leaf surface.

Identification and genomic sequence analysis of a new Spodoptera exigua multiple nucleopolyhedrovirus, SeMNPV-QD, isolated from Qingdao, China.

A new beet armyworm (Spodoptera exigua) multiple nucleopolyhedrovirus SeMNPV-QD was isolated from dead larvae in the field in Qingdao, Shandong, China. The virus has a polyhedron size of 1.39 ±â€¯0.28 µm, and typical virions contain one to seven nucleocapsids per envelope. SeMNPV-QD only infects the larvae of S. exigua; it does not infect larvae of S. litura, Agrotis ipsilon, A. segetum, Bombyx mori, Hyphantria cunea, or Stilpnotia salicis, and thus, has higher host specificity. SeMNPV-QD has a circular double-stranded DNA genome of 128,525 bp with a GC content of 37.41% and 127 putative open reading frames (ORF), each of which encodes more than 50 amino acids. These were identified and annotated in the SeMNPV-QD genome, accounting for 87.53% of the whole genome. Phylogenetic analysis of 38 core genes of the baculovirus confirmed that SeMNPV-QD is a Group II Alphabaculovirus and is most closely related to SeMNPV American isolate (SeMNPV-US1), S. litura nucleopolyhedrovirus II (SpliNPV-II), S. frugiperda multiple nucleopolyhedrovirus (SfMNPV), A. segetum nucleopolyhedrovirus (AgseNPV), A. segetum nucleopolyhedrovirus B (AgseNPV-B) and A. ipsilon multiple nucleopolyhedrovirus (AgipNPV). The pairwise distance of the nucleotide sequences of lef-8, lef-9, polh and concatenated lef-8/lef-9/polh fragments between SeMNPV-QD and several sister viruses mentioned above were all above 0.05 substitutions/site. SeMNPV-QD has 123 ORFs similar to those of SeMNPV-US1, and the genomic similarity was 45.8%. Compared to SeMNPV-US1, SeMNPV-QD has four additional ORFs such as two baculovirus repeat ORF (bro) genes, bro-1, bro-2, orf39 and orf95, but lacks 17 ORFs that have no effect on viral transcription and replication. The above results indicate that SeMNPV-QD is a new species of S. exigua multiple nucleopolyhedrovirus.

Serumfree culture of the suspension cell line QB-Tn9-4s of the cabbage looper, Trichoplusia ni, is highly productive for virus replication and recombinant protein expression.

Serumfree cultures of insect cells play an important role in the fields of protein engineering, medicine, and biology. In this paper, the suspension cell line QB-Tn9-4s of Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae) was successfully adapted to serumfree Sf-900 III medium and passaged for 52 generations. The adapted QB-Tn9-4s cells grew faster. Their population doubling time shortened from 27.4 hr in serum-containing medium to 24.1 hr, and their maximal density increased by 1.83-fold, reaching 3.50 ×10(6) cells/mL in serumfree culture in T-flasks. The cells readily adapted to spinner culture, with maximum cell density of 4.40 × 10(6) cells/mL in a spinner flask. Although the infection rate of Autographa californica multiple nucleopolyhedrovirus and production of occlusion bodies (OBs) of the adapted QB-Tn9-4s cells were 91.0% and 85.4 OBs/cell, respectively, similar to those of QB-Tn9-4s cells cultured in serum-containing medium and control BTI-Tn5B1-4 cells, their budded virus titer was 4.97 ×10(7) TCID50/mL, significantly higher than those of the latter two. In addition, the expression levels of ß-galactosidase at six days postinfection and secreted alkaline phosphatase at seven days postinfection in the adapted QB-Tn9-4s cells reached 2.98 ± 0.15×10(4) IU/mL and 3.34 ± 0.13 IU/mL, respectively, significantly higher than those of QB-Tn9-4s and control BTI-Tn5B1-4 cultured in serum-containing media. The above findings establish a foundation for industrial production of virus and recombinant proteins in QB-Tn9-4s serumfree culture.

Efficacy of Spleen-and-Stomach-Tonifying, Yin-Fire-Purging, and Yang-Raising Decoction Derived from the Trimethylamine N-Oxide Metabolic Pathway of Intestinal Microbiota on Macrovascular Lesions Caused by Type 2 Diabetes Mellitus.

Objective: We aimed to analyze the mechanisms underlying spleen-and-stomach-tonifying, yin-fire-purging, and yang-raising decoction derived from the trimethylamine N-oxide (TMAO) metabolic pathway of intestinal microbiota in the treatment of macrovascular lesions caused by type 2 diabetes mellitus (T2DM). Methods: Hartley-guinea pigs were randomly divided into 3 groups-the blank, model, and intervention groups. The T2DM combined with atherosclerosis guinea pig models were established in the model and intervention groups. After successful modeling, spleen-and-stomach-tonifying, yin-fire-purging, and yang-raising decoction were administered intragastrically to the intervention group, while the same volume of normal saline was administered via gavage to the blank and model groups. After 6 weeks of continuous gavage, guinea pigs were sacrificed in all groups, the colon contents were obtained, and the diversity and structural differences of intestinal microbiota were analyzed via bioinformatics. Serum was collected to detect differences in lipids, TMAO, oxidative stress, and inflammation markers between groups. Results: Compared to the blank group, the species diversity of the intestinal microbiota in the model and intervention groups was significantly reduced. Based on the results of Analysis of Similarities and Multiple Response Permutation Procedure, the microbiota structure of the intervention group was closer to that of the blank group. After modeling, the blood lipid levels of guinea pigs increased significantly, and drug intervention significantly reduced the levels of TC, TG, and LDL-C (P < 0.05). TMAO expression was significantly increased after modeling (P < 0.05), while drug intervention reduced TMAO expression (P < 0.05). Compared to the model group, drug intervention significantly increased the concentrations of SOD while decreasing the concentrations of MDA, ICAM-1, VCAM-1, IL-6, and hs-CRP. Conclusion: Spleen-and-stomach-tonifying, yin-fire-purging, and yang-raising decoction can reduce the risk of macrovascular lesions in T2DM, and its mechanism may be associated with its ability to regulate the TMAO metabolic pathway of intestinal microbiota.

Effects of different cellular and subcellular characteristics on the atmospheric Pb uptake, distribution and morphology in Tillandsia usneoides leaves.

Lead (Pb) is a widespread highly toxic and persistent environmental pollutant. Plant leaves play a key role in accumulating atmospheric Pb, but its distribution in different cells and subcellular structures and the factors affecting it have been little studied. Here, Tillandsia usneoides, an indicator plant for atmospheric heavy metals, was treated with an aerosol generation device to analyze Pb contents in different cells (three types of cells in leaf surface scales, epidermal cells, mesophyll cells, vascular bundle cells), subcellular structures (cell wall, cell membrane, vacuoles, and organelles) and cell wall components (pectin, hemicellulose 1 and 2, and cellulose). Results show the different cells of T. usneoides leaves play distinct roles in the process of Pb retention. The outermost wing cells are structures that capture external pollutants, while mesophyll cells, as the aggregation site after material transport, ring cells, disc cells, epidermal cells, and vascular cells are material transporters. Pb was only detected in the cell wall and pectin, indicating the cell wall was the dominant subcellular structure for Pb retention, while pectin was the main component affecting Pb retention. FTIR analysis of cell wall components indicated the esterified carboxyl (CO) functional group in pectin may function in absorbing Pb. Pb entered leaf cells mainly in the form of low toxicity and activity to enhance its resistance.

Retention properties and mechanism of agricultural waste maize whisker on atmospheric mercury.

Mercury (Hg) is a global pollutant transmitted mainly through the atmosphere, posing a serious threat to biological survival and human health. Porous materials, with high specific surface area, high porosity, and high adsorption, are particularly suitable for the purification of atmospheric Hg mixtures. However, plant porous materials are rarely directly used for atmospheric Hg purification. In this study, the properties and mechanism of maize whisker in removing atmospheric Hg were analyzed. The results show that the Hg content in the whiskers increases significantly as the initial Hg concentration increases, and 79.38% Hg can be removed by 0.2 g maize whiskers after 1 h exposure when the initial Hg concentration is 0.1 µg m-3, indicating that maize whiskers can accumulate atmospheric Hg rapidly and effectively. The hole diameter of the maize whisker is between 0.83 and 3.06 µm, which is suitable for the adsorption of small substances. Correlation analysis shows that maize whiskers have a significant correlation between atmospheric Hg retention and its specific surface area, pore size, medium pore ratio, and micropore ratio, suggesting that the maize whisker hole feature has a significant influence on its ability to retain atmospheric Hg. Compared with the energy profiles before and after Hg treatment, the peak of Mg decreased after Hg adsorption. Fourier infrared spectrometer analysis suggests that functional groups such as -OH, -COOH, and -O- are involved in the adsorption process. The change in pH value shows an obvious effect on the overall change in zeta potential in the adsorption process. Therefore, a variety of mechanisms, including physical adsorption, electrostatic adsorption, complexation, chelation, and ion exchange, are involved in Hg retention with the maize whisker. This study reveals the important potential value of agricultural waste maize whiskers in the purification of atmospheric heavy metal Hg.

Hormesis in the heavy metal accumulator plant Tillandsia ionantha under Cd exposure: Frequency and function of different biomarkers.

Cadmium is one of the most biotoxic substances among all heavy metals, but an increasing number of studies indicate that low-dose Cd can induce hormesis in some plants. However, the frequency of hormesis in various biomarkers (molecular, resistance, and damage markers) and their associated function in hormesis-generation are poorly understood. In this study, the heavy metal accumulator plant Tillandsia ionantha Planch. was exposed to 5 mM CdCl2 with 6 different time periods. The trends of 18 biomarkers after Cd exposure were detected. The percentage for all non-monophasic responses based on dose-response modeling was higher (50 %), in which seven (38.89 %) biomarkers showed hormesis, indicating that hormesis effect can commonly occur in this plant. However, the occurrence frequency of hormesis in different types of biomarkers was different. Six Cd resistance genes, glutathione (GSH) among 6 resistance markers, and 0 damage markers showed hormesis. Factor analysis further showed that the 6 Cd resistance genes and GSH were positively intercorrelated in the first principal component. Therefore, heavy metal resistance genes and GSH may play an important role in the generation of hormesis. Our experiment shows that time-dependent non-monophasic responses, including hormesis, are activated by considerably high concentrations of Cd, presenting a strategy to cope with and potentially reduce the anticipated damage as the dose of stress increases over time.

Antiviral function of peptidoglycan recognition protein in Spodoptera exigua (Lepidoptera: Noctuidae).

Peptidoglycan recognition proteins (PGRPs) are a class of molecules that play a critical role in insect immunity. Understanding the function of PGRPs is important to improve the efficiency of microbial insecticides. In this study, we investigated the role of PGRP-LB (a long type PGRP) in insect immunity against viruses using Spodoptera exigua and Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) as an insect-virus model. We cloned and identified a PGRP-LB gene from S. exigua; the gene consisted of 7 exons that encoded a polypeptide of 234 amino acids with a signal peptide and a typical amidase domain. Expression analysis revealed that the abundance of SePGRP-LB transcripts in the fat body was greater than in other tissues. Overexpression of SePGRP-LB resulted in a significant decrease of 49% in the rate of SeMNPV-infected cells. In addition, the multiplication of SeMNPV was significantly decreased: a decrease of 79% in the production of occlusion-derived virion (ODV), and a maximum decrease of 50% in the production of budded virion (BV). In contrast, silencing of SePGRP-LB expression by RNA interference resulted in a significant 1.65-fold increase in the rate of SeMNPV-infected cells, a significant 0.54-fold increase in ODV production, a maximum 1.57-fold increase in BV production, and the larval survival dropped to 21%. Our findings show that SePGRP-LB has an antiviral function against SeMNPV, and therefore this gene may provide a target for lepidopteran pest control using virus insecticides.

Potential of monitoring nuclides with the epiphyte Tillandsia usneoides: Uptake and localization of 133Cs.

Epiphytic Tillandsia plants are efficient air pollution biomonitors and traditionally used to monitor atmospheric heavy metal pollution, but rarely nuclides monitoring. Here we evaluated the potential of Tillandsia usneoides for monitoring (133)Cs and investigated if Cs was trapped by the plant external surface structures. The results showed that T. usneoides was able to survive relatively high Cs stress. With the increase of Cs solution concentration, the total of Cs in plants increased significantly, which suggests that the plants could accumulate Cs quickly and effectively. Therefore, T. usneoides has considerable potential for monitoring Cs polluted environments. In addition, scanning electron microscopy (SEM) and energy dispersive spectrometer (EDS) analysis showed that Cs was detected in each type of cells in foliar trichomes, and the ratio of Cs in the internal disc cell was higher than that in ring cell and wing cell, which indicates that the mechanism of adsorption Cs in Tillandsia has an active component.

Atmospheric Pb induced hormesis in the accumulator plant Tillandsia usneoides.

While numerous studies reported hormesis in plants exposed to heavy metals, metals were commonly added in the growth substrate (e.g. soil or solution). The potential of heavy metals in the atmosphere to induce hormesis in plants, however, remains unknown. In this study, we exposed the widely-used accumulator plant Tillandsia usneoides to 10 atmospheric Pb concentrations (0-25.6 µg·m-3) for 6 or 12 h. Three types of dose-response relationships between different response endpoints (biomarkers) and Pb concentrations were found for T. usneoides. The first was a monophasic dose response, in which the response increased linearly with increasing Pb concentrations, as seen for metallothionein (MT) content after a 6-h exposure. The second and dominating type was a biphasic-hormetic dose response, exhibited by malondialdehyde (MDA), superoxide anion radical (O2-), and superoxide dismutase (SOD) after 6 or 12 h of exposure and by glutathione (GSH) and MT content after 12 h of treatment. The third type was a triphasic dose response, as seen for leaf electric conductivity after 6 or 12 h of exposure and GSH after 6 h of exposure. This finding suggests that Pb inhibited the response of T. usneoides at very low concentrations, stimulated it at low-to-moderate concentrations, and inhibited it at higher concentrations. Our results demonstrate diverse adaptation mechanisms of plants to stress, in the framework of which alternating between up- and down-regulation of biomarkers is at play when responding to different levels of toxicants. The emergence of the triphasic dose response will further enhance the understanding of time-dependent hormesis.

Cellular and subcellular distribution and factors influencing the accumulation of atmospheric Hg in Tillandsia usneoides leaves.

Atmospheric Hg is a highly toxic heavy metal with bioaccumulative properties. However, relatively few studies have focused on the distribution of Hg in cellular and subcellular structures of plants and factors influencing its accumulation. In this study, we selected Tillandsia usneoides, which is a widely used bioindicator for Hg, to analyze the concentration of Hg in different cells (foliar trichomes, epidermal cells, mesophyll cells, and vascular bundle cells), different subcellular structures (cell wall, cell membrane, vacuoles, and organelles) and different cell wall components (pectin, hemicellulose 1, and hemicellulose 2). It was determined that Hg was present in different types of cells, but there was no significant difference, suggesting that atmospheric Hg circulates dynamically in the surface and internal structural cells of T. usneoides leaves. Subcellular analysis showed that as Hg concentration increased, more Hg accumulated in the vacuoles and cell wall through the compartmentalization mechanism. Hemicellulose had the highest content of Hg, indicating that it is the primary Hg-binding component of the cell wall. The FTIR analysis results showed that after the Hg treatment, the cell wall -OH and COO- absorption peaks changed most significantly, indicating that these functional groups play a vital role in the Hg accumulation process.

Biomarker Responses of Spanish Moss Tillandsia usneoides to Atmospheric Hg and Hormesis in This Species.

Hg is an environmental pollutant with severe biotoxicity. Epiphytic Tillandsia species, especially Spanish moss T. usneoides, are widely used as the bioindicator of Hg pollution. However, the effects of different Hg concentrations on Tillandsia have been rarely studied and the occurrence of hormesis in Tillandsia species has not been determined. In this study, T. usneoides was subjected to stress induced by 15 concentrations of gaseous Hg ranging from 0 to 1.8 µg m-3 through a misting system and then Hg content and eight common biomarkers in leaves were measured. The results showed that leaf Hg content significantly increased with Hg concentration, showing a linear relationship. However, there were no obvious mortality symptoms, indicating that T. usneoides showed strong resistance to Hg. Conversely, there were no simple linear relationships between changes in various biomarkers following Hg treatment of T. usneoides and Hg concentration. With increasing Hg concentration, malondialdehyde (MDA) content did not change significantly, superoxide anion radical content decreased gradually, superoxide dismutase (SOD) content decreased to the bottom and then bounced back, electrical conductivity increased, and glutathione (GSH) and metallothionein (MT) content increased to the peak and then dropped. The coefficient of determination of the dose-effect curves between SOD, GSH, and MT contents and Hg concentration was high, and the dose-effect relationship varied with hormesis. The present study is first to confirm hormesis induced by heavy metal pollution in Tillandsia species.

Establishment and characterization of the Bactrocera dorsalis (Diptera: Tephritidae) embryonic cell line QAU-Bd-E-2.

In this study, we successfully established a Bactrocera dorsalis (Diptera: Tephritidae) embryonic cell line, i.e., QAU-Bd-E-2, from the insect eggs. The cells have been stably passaged for more than 60 times in TNM-FH medium with 10% fetal bovine serum (FBS). QAU-Bd-E-2 cells are adherent cells. Most of the cells were round, spindle-shaped, and rod-shaped. Round cells accounted for 82.3%, with a diameter of 13.9 ± 2.6 µm; spindle-shaped cells accounted for 9.8%, with the size of 51.2 ± 11.2 µm × 10.3 ± 3.1 µm; the rod-shaped cells accounted for 7.9%, with the size of 35.2 ± 9.4 µm × 12.0 ± 2.5 µm. The mitochondrial cytochrome oxidase I subunit (CoI) gene from QAU-Bd-E-2 cells was amplified, and the 657 bp fragment had a 100% similarity with the CoI gene of B. dorsalis, suggesting that the cell line was derived from B. dorsalis. The chromosome number of QAU-Bd-E-2 cells was mostly 12, which is the same as the B. dorsalis chromosome number. The cell density of QAU-Bd-E-2 cells reached the maximum (3.4 × 106 cells/mL) at 192 h, and the population doubling time was 31.9 h. Bactrocera dorsalis cripavirus (BdCV) could replicate in QAU-Bd-E-2 cells, suggesting that this cell line could be used for in-depth study of the relationship between virus and host.

Resuspension of settled atmospheric particulate matter on plant leaves determined by wind and leaf surface characteristics.

Atmospheric particulate matter (APM) is temporarily settled on the leaf surface of plants and will return to the air via the resuspension process under certain meteorological conditions. How leaf surface characteristics affect the resuspension of settled APM on the leaf surface has been rarely studied. Therefore, the resuspension of APM after settling on plant leaves was analyzed using four common urban greening species, including Prunus triloba, Platanus acerifolia, Lonicera maackii, and Cercis chinensis. The results show that the leaf hair density has a significantly positive correlation with the maximum particulate matter (PM) retention and natural PM retention (p < 0.05). Under the same wind speed, the proportions of the resuspended PM that settled on the leaf surfaces of the four plant species increase with the wind blowing time. During the same wind blowing time, the resuspension rate of the settled PM on leaf surfaces of P. triloba, P. acerifolia, and L. maackii increase with the wind speed. The leaf hair and stomatal density is negatively correlated to the resuspension rate of PM under the wind speed of 1 m s-1 (p < 0.05), and the stomatal density is also negatively correlated to the resuspension rate of PM under the wind speed of 5 m s-1 for 10 min or 20 min (p < 0.05). However, as the wind speed further increase, the leaf characteristics are no longer correlated to the resuspension rate of PM (p > 0.05). These results indicate that when the wind force (wind speed + wind blowing time) is small, the stomatal density and leaf hair density have a significant effect on APM resuspension. When the wind force is large, the influence of leaf surface structure on APM resuspension becomes less profound. APM resuspension is comprehensively affected by the external wind and the leaf surface characteristics, and these two factors jointly determine the fate of the PM after it settles on leaves.

Effects of Foliar Trichomes on the Accumulation of Atmospheric Particulates in Tillandsia Brachycaulos.

Foliar trichomes are specialized structures that have first contact with atmospheric particulate matter (PM), while their effects on PM accumulation are rarely investigated. In this study, epiphytic Tillandsia brachycaulos with foliar trichomes was investigated. Trichomes were removed artificially to investigate PM adsorption and resuspension. The results showed that the maximum PM (13.94±0.20 g•m-2) and natural PM content (10.65±1.10 g•m-2) on T. brachycaulos samples with foliar trichomes was significantly higher than those without foliar trichomes. After PM deposition on the leaf surface, more than 85% of PM was dispersed by wind in plants without trichomes, significantly higher than those with trichomes (30.2%). Therefore, the effects of foliar trichomes on PM were reflected not only on PM adsorption, but on PM resuspension. Foliar trichomes can decrease PM resuspension falling on the leaf surface and promote the accumulation of these particles.

Genetic and physiological effects of the natural radioactive gas radon on the epiphytic plant Tillandsia brachycaulos.

Radon (222Rn) is the most abundant natural radioactive gas in nature and triggers carcinogenesis. Few reports exist on whether radon can damage plants as it does animals. Therefore, we chose Tillandsia brachycaulos, a common indicator plant, as the material to detect the physiological and genetic changes caused by radon. With an increase in radon concentration, DNA indices (tail length, tail DNA, tail moment and Olive tail moment) from the comet assay and malondialdehyde (MDA) content increased significantly, suggesting that T. brachycaulos inevitably suffered from radiation damage. However, neither the leaf relative conductivity nor the soluble protein content changed significantly with radon fumigation, and no dose-dependent effect existed between the chlorophyll content and radon concentration, indicating that T. brachycaulos had resistance to radon stress. Foliar trichomes most likely excluded the pollutant from plants because DNA damage in T. brachycaulos with trichomes manually removed was considerably greater than that with trichomes. Moreover, the antioxidant enzyme system further reduced the damage of radon to plants because the activity of superoxide dismutase (SOD) increased significantly with the radon concentration.

Uptake of the natural radioactive gas radon by an epiphytic plant.

Radon (222Rn) is a natural radioactive gas and the major radioactive contributor to human exposure. The present effective ways to control Rn contamination are ventilation and adsorption with activated carbon. Plants are believed to be negligible in reducing airborne Rn. Here, we found epiphytic Tillandsia brachycaulos (Bromeliaceae) was effective in reducing airborne Rn via the leaves. Rn concentrations in the Rn chamber after Tillandsia plant treatments decreased more than those in the natural situation. The specialized foliar trichomes densely covering Tillandsia leaves play a major role in the uptake of Rn because the amplified rough leaf surface area facilitates deposition of Rn progeny particles and the powdery epicuticular wax layer of foliar trichomes uptakes liposoluble Rn. The results provide us a new ecological strategy for Rn contamination control, and movable epiphytic Tillandsia plants can be applied widely in Rn removal systems.