Signal-Enhanced Detection of Multiplexed Cardiac Biomarkers by a Paper-Based Fluorogenic Immunodevice Integrated with Zinc Oxide Nanowires.

Using a single test to comprehensively evaluate multiple cardiac biomarkers for early diagnosis and prevention of acute myocardial infarction (AMI) has faced enormous challenges. Here, we have developed paper-based fluorogenic immunodevices for multiplexed detection of three cardiac biomarkers, namely, human heart-type fatty acid binding protein (FABP), cardiac troponin I (cTnI), and myoglobin, simultaneously. The detection is based on a strategy using zinc oxide nanowires (ZnO NWs) to enhance fluorescence signals (∼5-fold compared to that on pure paper). The immunodevices showed high sensitivity and selectivity for FABP, cTnI, and myoglobin with detection limits of 1.36 ng/mL, 1.00 ng/mL, and 2.38 ng/mL, respectively. Additionally, the paper-based immunoassay was rapid (∼5 min to complete the test) and portable (using a homemade chamber with a smartphone and an ultraviolet lamp). The developed devices integrated with ZnO NWs enable quantitative, sensitive, and simultaneous detection of multiple cardiac biomarkers in point-of-care settings, which provides a useful approach for monitoring AMI diseases and may be extended to other medical diagnostics and environmental assessments.

A transparent paper-based platform for multiplexed bioassays by wavelength-dependent absorbance/transmittance.

This communication describes the rational design of a transparent paper-based chemosensing platform for multi-target detection by wavelength-dependent absorbance/transmittance. The platform was successfully applied in the examination of bovine serum albumin (BSA) and cholesterol in serum with a low detection limit of 0.1 µM and 0.1 mM, respectively. With low cost and high sensitivity, the paper-based platform shows great promise for multiplexed bioassays.

Using magnetic levitation for density-based detection of cooking oils.

Adulterated cooking oils and the repeated use of frying cooking oils are harmful to human health. Current techniques for the quality control of cooking oils such as gas chromatography and high performance liquid chromatography usually require expensive facilities, and they are complicated to operate. This paper describes a simple technique that uses magnetic levitation (MagLev) to analyse cooking oils based on density. We have demonstrated the application of MagLev to detect the quality of cooking oil by simulating adulterated sesame oil using peanut oil and frying soybean oil for different times. We have also demonstrated the use of MagLev to differentiate secondhand cooking oil from certified cooking oil. MagLev provides a portable and inexpensive method for the on-site inspection of cooking oils, and it may be extended to many other applications in food safety, environmental monitoring, medical diagnosis, and so on. The advantages of high sensitivity, low cost, and convenience of operation make MagLev especially useful for in situ applications in resource-limited settings.

Paper-based fluorescent immunoassay for highly sensitive and selective detection of norfloxacin in milk at picogram level.

Norfloxacin (NOR) in milk may influence mammalian cell replication and bring about a decrease in the efficiency for treating infection in humans. However, current techniques for detecting NOR usually require expensive instruments and trained personnel. In this work, we have developed a low-cost and simple method via paper-based fluorescent immunoassay for highly sensitive and selective detection of NOR in milk at picogram level. The NOR monoclonal antibody labeled with quantum dots is used as a detection probe to recognize the corresponding NOR, which can quantitatively detect NOR on paper-based devices. The detection limits in aqueous solution and milk are 1 pg/mL and 10 pg/mL, respectively. The developed paper-based method provides a cheap, sensitive, eco-friendly, and rapid approach for quantitative detection of trace NOR in milk, which may find wide applications in food safety inspection. Noteworthy, the method is especially suitable for applications at resource-limited and on-site settings.