RESUMO
The objective of the study described in this article was to characterize the antimicrobial resistance profiles among E. coli strains isolated from cohabitant pets and humans, evaluating the concurrent colonization of pets, owners, and home surfaces by bacteria carrying the same antimicrobial-resistant genes. The authors also intended to assess whether household surfaces and objects could contribute to the within-household antimicrobial-resistant gene diffusion between human and animal cohabitants. A total of 124 E. coli strains were isolated displaying 24 different phenotypic patterns with a remarkable percentage of multiresistant ones. The same resistance patterns were isolated from the dog's urine, mouth, the laundry floor, the refrigerator door, and the dog's food bowl. Some other multiresistant phenotypes, as long as resistant genes, were found repeatedly in different inhabitants and surfaces of the house. Direct, close contact between all the cohabitants and the touch of contaminated household surfaces and objects could be an explanation for these observations.
Assuntos
Farmacorresistência Bacteriana/genética , Resistência a Múltiplos Medicamentos/genética , Contaminação de Equipamentos , Infecções por Escherichia coli/transmissão , Escherichia coli/efeitos dos fármacos , Animais de Estimação/microbiologia , Animais , Gatos/microbiologia , Pré-Escolar , Cães/microbiologia , Escherichia coli/genética , Infecções por Escherichia coli/veterinária , Feminino , Genótipo , Humanos , Masculino , Testes de Sensibilidade Microbiana , Fenótipo , Portugal , Recidiva , Infecções Urinárias/transmissão , Infecções Urinárias/veterináriaRESUMO
Recent studies of canine malignant mammary tumours suggest that reduction of E-cadherin and/or beta-catenin correlates with invasive behaviour and lymph node metastasis. The aims of this study were to examine the interrelationships between the expression of E-cadherin and beta-catenin, and the relationship between the expression of E-cadherin and/or beta-catenin and the mode of growth and metastatic capacity of canine malignant mammary tumours. 90 spontaneous malignant tumours and local and regional lymph nodes were studied. A significant relationship was evidenced between membranous expression of E-cadherin and beta-catenin (p=0.0027), but not between E-cadherin and cytoplasmic beta-catenin. Only E-cadherin as a separate factor was significantly related to tumour invasion (p=0.0072) and lymph node metastasis (p=0.0001). Neither membranous nor cytoplasmic beta-catenin expression was significantly related to either of these phenomena.
Assuntos
Caderinas/análise , Neoplasias Mamárias Animais/patologia , beta Catenina/análise , Animais , Caderinas/metabolismo , Cães , Regulação para Baixo , Feminino , Linfonodos/patologia , Metástase Linfática , Neoplasias Mamárias Animais/química , Invasividade Neoplásica , beta Catenina/metabolismoRESUMO
The relationship between MIB-1 labelling indices (LI), as detected by immunohistochemical methods, and other clinico-pathological characteristics was studied in a series of 77 malignant mammary tumours surgically removed from 47 female dogs. The immunostaining was assessed on the basis of the estimated percentage of positive cells in the areas of highest labelling. Multivariate logistic regression demonstrated no influence of breed, age, previous pregnancies, previous progestin administration, histological type or location of the tumour on MIB-1 LI. MIB-1 LI was significantly related to the size of the tumour, necrosis, invasive growth and histological grade, but not with ulceration, lymph node metastasis, skin fixation or E-cadherin expression. The significant relationship between MIB-1 LI and other known factors of poor prognosis suggests that a high LI may have prognostic value in canine malignant mammary tumours.