RESUMO
A fatal human case of Duvenhage virus (DUVV) infection in a Dutch traveller who had returned from Kenya was reported in 2007. She exhibited classical symptoms of rabies encephalitis with distinct pathological findings. In the present study we describe the isolation and characterization of DUVV in vitro and its passage in BALB/c mice. The virus proved to be neuroinvasive in both juvenile and adult mice, resulting in about 50% lethality upon peripheral infection. Clinical signs in infected mice were those of classical rabies. However, the distribution of viral antigen expression in the brain differed from that of classical rabies virus infection and neither inclusion bodies nor neuronal necrosis were observed. This is the first study to describe the in vitro and in vivo isolation and characterization of DUVV.
Assuntos
Encefalite Viral/virologia , Lyssavirus/isolamento & purificação , Lyssavirus/patogenicidade , Infecções por Rhabdoviridae/virologia , Animais , Antígenos Virais/imunologia , Sequência de Bases , Encéfalo/patologia , Encéfalo/virologia , Linhagem Celular Tumoral , Cricetinae , Encefalite Viral/diagnóstico , Encefalite Viral/imunologia , Feminino , Humanos , Lyssavirus/classificação , Lyssavirus/genética , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , RNA Viral/genética , Infecções por Rhabdoviridae/diagnóstico , Infecções por Rhabdoviridae/imunologia , Análise de Sequência de RNA , Inoculações Seriadas , ViagemRESUMO
A different clinical picture and therapeutic response were observed when data from Leishmania major-infected Dutch military personnel stationed in southern (N = 8) and northern (N = 169) Afghanistan were analyzed. Clinical presentation of cutaneous leishmaniasis in personnel in the south was milder and seemed to respond better to antileishmanial treatment; molecular analyses of parasite isolates seem to indicate that these differences may be genetic.
Assuntos
Leishmania major/patogenicidade , Leishmaniose Cutânea/parasitologia , Afeganistão , Genótipo , HumanosRESUMO
Cutaneous leishmaniasis in Surinam is generally caused by infection by Leishmania guyanensis. We report three cases of infection with Leishmania (Viannia) naiffi, a Leishmania species not described from Surinam before. Treatment with pentamidine proved to be effective.