RESUMO
Exposure of human cells to genotoxic agents induces various signaling pathways involved in the execution of stress- and DNA-damage responses. Inappropriate functioning of the DNA-damage response to ionizing radiation (IR) is associated with the human diseases ataxia-telangiectasia (A-T) and Nijmegen Breakage syndrome (NBS). Here, we show that IR efficiently induces Jun/ATF transcription factor activity in normal human diploid fibroblasts, but not in fibroblasts derived from A-T and NBS patients. IR was found to enhance the expression of c-Jun and, in particular, ATF3, but, in contrast to various other stress stimuli, did not induce the expression of c-Fos. Using specific inhibitors, we found that the ATM- and Nibrin1-dependent activation of ATF3 does neither require p53 nor reactive oxygen species, but is dependent on the p38 and JNK MAPkinases. Via these kinases, IR activates ATF-2, one of the transcription factors acting on the atf3 promoter. The activation of ATF-2 by IR resembles ATF-2 activation by certain growth factors, since IR mainly induced the second step of ATF-2 phosphorylation via the stress-inducible MAPkinases, phosphorylation of Thr69. As IR does not enhance ATF-2 phosphorylation in ATM and Nibrin1-deficient cells, both ATF-2 and ATF3 seem to play an important role in the protective response of human cells to IR.
Assuntos
Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/metabolismo , Sistema de Sinalização das MAP Quinases , Radiação Ionizante , Transdução de Sinais , Fatores de Transcrição/metabolismo , Fator 2 Ativador da Transcrição , Fator 3 Ativador da Transcrição , Proteínas Mutadas de Ataxia Telangiectasia , Northern Blotting , Western Blotting , Proteínas de Ciclo Celular/metabolismo , Células Cultivadas , Dano ao DNA , Proteínas de Ligação a DNA , Fibroblastos/metabolismo , Fibroblastos/efeitos da radiação , Humanos , Proteínas Quinases JNK Ativadas por Mitógeno , Cinética , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Proteínas Nucleares/metabolismo , Estresse Oxidativo , Fosforilação , Regiões Promotoras Genéticas , Proteínas Serina-Treonina Quinases/metabolismo , Fatores de Tempo , Fatores de Transcrição/genética , Transcrição Gênica , Proteína Supressora de Tumor p53/metabolismo , Proteínas Supressoras de Tumor , Proteínas Quinases p38 Ativadas por MitógenoRESUMO
Recombination can result in genetic instability, and thus constitutes an important factor in the carcinogenic conversion of mammalian cells. Here we describe the occurrence of UV-stimulated recombination called enhanced recombination (EREC), measured with the use of Herpes Simplex Viruses type 1 mutants. In normal diploid human cells, EREC is induced by UV-C, mitomycin C and ENU, but not by X-ray or MMS. The kinetics of induction of EREC is similar to that of other SOS-like responses such as enhanced reactivation (ER) and enhanced mutagenesis (EM). In contrast to the latter responses, EREC is induced to higher levels and persists for longer periods in DNA repair deficient fibroblasts derived from xeroderma pigmentosum (XP), Cockayne syndrome (CS) and Trichothiodystrophy (TTD) patients. This observation indicates that EREC is a distinct SOS-like response. Apparently, the presence of unrepaired DNA lesions in the host genome is a strongly inducing signal for EREC. On the other hand, in cells derived from patients suffering from Bloom, Werner or Rothmund-Thomson syndrome (RTS) the EREC response is absent. These data indicate that determining EREC is a useful assay to investigate diploid human fibroblasts for abnormalities in UV-stimulated recombination.
Assuntos
Síndrome de Cockayne/genética , Reparo do DNA , Doenças do Cabelo/genética , Recombinação Genética , Xeroderma Pigmentoso/genética , Animais , Células Cultivadas , Cricetinae , Cricetulus , Dano ao DNA , Relação Dose-Resposta à Radiação , Fibroblastos/metabolismo , Fibroblastos/efeitos da radiação , Herpesvirus Humano 1/genética , Humanos , Cinética , Mutação , Pele/citologia , Fatores de Tempo , Raios UltravioletaRESUMO
Treatment of cells with genotoxic agents affects protein degradation in both positive and negative ways. Exposure of S. cerevisiae to the alkylating agent MMS resulted in activation of genes that are involved in ubiquitin- and 26S proteasome-dependent protein degradation. This process partially overlaps with the activation of the ER-associated protein degradation pathway. The DNA repair protein Rad23p and its mammalian homologues have been shown to inhibit degradation of specific substrates in response to DNA damage. Particularly the recently identified inhibition of degradation by mouse Rad23 protein (mHR23) of the associated nucleotide excision repair protein XPC was shown to stimulate DNA repair.Recently, it was shown that Rad23p and the mouse homologue mHR23B also associate with Png1p, a deglycosylation enzyme. Png1p-mediated deglycosylation plays a role in ER-associated protein degradation after accumulation of malfolded proteins in the endoplasmic reticulum. Thus, if stabilization of proteins that are associated with the C-terminus of Rad23p is a general phenomenon, then Rad23 might be implicated in the stimulation of ER-associated protein degradation as well. Interestingly, the recently identified HHR23-like protein Mif1 is also thought to play a role in ER-associated protein degradation. The MIF1 gene is strongly activated in response to ER-stress. Mif1 contains a ubiquitin-like domain which is most probably involved in binding to S5a, a subunit of the 19S regulatory complex of the 26S proteasome. On the basis of its localization in the ER-membrane, it is hypothesized that Mif1 could play a role in the translocation of the 26S proteasome towards the ER-membrane, thereby enhancing ER-associated protein degradation.
Assuntos
Proteínas de Bactérias , Dano ao DNA/fisiologia , Reparo do DNA , Proteínas de Ligação a DNA/metabolismo , Proteínas Fúngicas/metabolismo , Peptídeo Hidrolases/metabolismo , Complexo de Endopeptidases do Proteassoma , Proteínas Serina-Treonina Quinases , Proteínas/metabolismo , Proteínas de Saccharomyces cerevisiae , Animais , Dano ao DNA/efeitos dos fármacos , Enzimas Reparadoras do DNA , Proteínas de Ligação a DNA/genética , Proteínas Fúngicas/genética , Humanos , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/metabolismo , Metanossulfonato de Metila/farmacologia , Poliubiquitina/metabolismo , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Subtilisinas/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismoAssuntos
Genes Transgênicos Suicidas/genética , Terapia Genética/efeitos adversos , Antineoplásicos/farmacocinética , Antineoplásicos/uso terapêutico , Antineoplásicos/toxicidade , Vetores Genéticos , Humanos , Mutação , Pró-Fármacos/farmacocinética , Pró-Fármacos/toxicidade , Segurança , Replicação Viral , Eliminação de Partículas ViraisRESUMO
Adenovirus type 12 (Ad12)-transformed baby rat kidney (BRK) cells are oncogenic in syngeneic immunocompetent rats in contrast to adenovirus type 5 (Ad5)-transformed BRK cells, which are not oncogenic in these animals. A significant factor contributing to the difference in oncogenicity may be the low levels of major histocompatibility complex (MHC) class I membrane expression in Ad12-transformed BRK cells as compared with those in Ad5-transformed BRK cells, which presumably results in escape from killing by cytotoxic T lymphocytes. Here we show that, in addition to the decreased levels of expression of the MHC class I heavy chain and the peptide transporter Tap-2, the expression levels of the chaperone Tapasin and the immunoproteasome components MECL-1, PA28-alpha, and PA28-beta also are much lower in Ad12- than in Ad5-transformed BRK cells. The low expression levels of these proteins may contribute to the escape from killing by cytotoxic T lymphocytes, because the generation of optimal peptides and loading of these peptides on MHC class I require these components. Increased levels of phosphorylated signal transducer and activator of transcription-1 protein and expression of IFN regulatory factor-7 were found in Ad5- versus Ad12-transformed BRK cells. Therefore, the critical alteration leading to the plethora of differences may be an interferon (-related) effect.