Ubiquitination of phosphatidylethanolamine in organellar membranes.

The covalent conjugation of ubiquitin family proteins is a widespread post-translational protein modification. In the ubiquitin family, the ATG8 subfamily is exceptional because it is conjugated mainly to phospholipids. However, it remains unknown whether other ubiquitin family proteins are also conjugated to phospholipids. Here, we report that ubiquitin is conjugated to phospholipids, mainly phosphatidylethanolamine (PE), in yeast and mammalian cells. Ubiquitinated PE (Ub-PE) accumulates at endosomes and the vacuole (or lysosomes), and its level increases during starvation. Ub-PE is also found in baculoviruses. In yeast, PE ubiquitination is catalyzed by the canonical ubiquitin system enzymes Uba1 (E1), Ubc4/5 (E2), and Tul1 (E3) and is reversed by Doa4. Liposomes containing Ub-PE recruit the ESCRT components Vps27-Hse1 and Vps23 in vitro. Ubiquitin-like NEDD8 and ISG15 are also conjugated to phospholipids. These findings suggest that the conjugation to membrane phospholipids is not specific to ATG8 but is a general feature of the ubiquitin family.

Msp1 Clears Mistargeted Proteins by Facilitating Their Transfer from Mitochondria to the ER.

Normal mitochondrial functions rely on optimized composition of their resident proteins, and proteins mistargeted to mitochondria need to be efficiently removed. Msp1, an AAA-ATPase in the mitochondrial outer membrane (OM), facilitates degradation of tail-anchored (TA) proteins mistargeted to the OM, yet how Msp1 cooperates with other factors to conduct this process was unclear. Here, we show that Msp1 recognizes substrate TA proteins and facilitates their transfer to the endoplasmic reticulum (ER). Doa10 in the ER membrane then ubiquitinates them with Ubc6 and Ubc7. Ubiquitinated substrates are extracted from the ER membrane by another AAA-ATPase in the cytosol, Cdc48, with Ufd1 and Npl4 for proteasomal degradation in the cytosol. Thus, Msp1 functions as an extractase that mediates clearance of mistargeted TA proteins by facilitating their transfer to the ER for protein quality control.

The Dfm1 Derlin Is Required for ERAD Retrotranslocation of Integral Membrane Proteins.

Endoplasmic reticulum (ER)-associated degradation (ERAD) removes misfolded proteins from the ER membrane and lumen by the ubiquitin-proteasome pathway. Retrotranslocation of ubiquitinated substrates to the cytosol is a universal feature of ERAD that requires the Cdc48 AAA-ATPase. Despite intense efforts, the mechanism of ER exit, particularly for integral membrane (ERAD-M) substrates, has remained unclear. Using a self-ubiquitinating substrate (SUS), which undergoes normal retrotranslocation independently of known ERAD factors, and the new SPOCK (single plate orf compendium kit) micro-library to query all yeast genes, we found the rhomboid derlin Dfm1 was required for retrotranslocation of both HRD and DOA ERAD pathway integral membrane substrates. Dfm1 recruited Cdc48 to the ER membrane with its unique SHP motifs, and it catalyzed substrate extraction through its conserved rhomboid motifs. Surprisingly, dfm1Δ can undergo rapid suppression, restoring wild-type ERAD-M. This unexpected suppression explained earlier studies ruling out Dfm1, and it revealed an ancillary ERAD-M retrotranslocation pathway requiring Hrd1.

Lipid biosynthesis perturbation impairs endoplasmic reticulum-associated degradation.

The relationship between lipid homeostasis and protein homeostasis (proteostasis) is complex and remains incompletely understood. We conducted a screen for genes required for efficient degradation of Deg1-Sec62, a model aberrant translocon-associated substrate of the endoplasmic reticulum (ER) ubiquitin ligase Hrd1, in Saccharomyces cerevisiae. This screen revealed that INO4 is required for efficient Deg1-Sec62 degradation. INO4 encodes one subunit of the Ino2/Ino4 heterodimeric transcription factor, which regulates expression of genes required for lipid biosynthesis. Deg1-Sec62 degradation was also impaired by mutation of genes encoding several enzymes mediating phospholipid and sterol biosynthesis. The degradation defect in ino4Δ yeast was rescued by supplementation with metabolites whose synthesis and uptake are mediated by Ino2/Ino4 targets. Stabilization of a panel of substrates of the Hrd1 and Doa10 ER ubiquitin ligases by INO4 deletion indicates ER protein quality control is generally sensitive to perturbed lipid homeostasis. Loss of INO4 sensitized yeast to proteotoxic stress, suggesting a broad requirement for lipid homeostasis in maintaining proteostasis. A better understanding of the dynamic relationship between lipid homeostasis and proteostasis may lead to improved understanding and treatment of several human diseases associated with altered lipid biosynthesis.

Ectopic RING activity at the ER membrane differentially impacts ERAD protein quality control pathways.

Endoplasmic reticulum-associated degradation (ERAD) is a protein quality control pathway that ensures misfolded proteins are removed from the ER and destroyed. In ERAD, membrane and luminal substrates are ubiquitylated by ER-resident RING-type E3 ubiquitin ligases, retrotranslocated into the cytosol, and degraded by the proteasome. Overexpression of ERAD factors is frequently used in yeast and mammalian cells to study this process. Here, we analyze the impact of ERAD E3 overexpression on substrate turnover in yeast, where there are three ERAD E3 complexes (Doa10, Hrd1, and Asi1-3). Elevated Doa10 or Hrd1 (but not Asi1) RING activity at the ER membrane resulting from protein overexpression inhibits the degradation of specific Doa10 substrates. The ERAD E2 ubiquitin-conjugating enzyme Ubc6 becomes limiting under these conditions, and UBC6 overexpression restores Ubc6-mediated ERAD. Using a subset of the dominant-negative mutants, which contain the Doa10 RING domain but lack the E2-binding region, we show that they induce degradation of membrane tail-anchored Ubc6 independently of endogenous Doa10 and the other ERAD E3 complexes. This remains true even if the cells lack the Dfm1 rhomboid pseudoprotease, which is also a proposed retrotranslocon. Hence, rogue RING activity at the ER membrane elicits a highly specific off-pathway defect in the Doa10 pathway, and the data point to an additional ERAD E3-independent retrotranslocation mechanism.

Improving the Accuracy of Direction of Arrival Estimation with Multiple Signal Inputs Using Deep Learning.

In this paper, an innovative cyclic noise reduction method and an improved CAPON algorithm (also the called minimum variance distortionless response (MVDR) algorithm) are proposed to improve the accuracy and reliability of DOA (direction of arrival) estimation. By processing the eigenvalues obtained from the covariance matrix of the received signal, the signal-to-noise ratio (SNR) can be increased by up to 5 dB by the cyclic noise reduction method, which will improve the DOA estimation accuracy. The improved CAPON algorithm has a convolution neural network (CNN) structure, whose input is the processed covariance matrix of the received signal, and the CAPON spectral value is used as the training label to obtain the estimated spatial spectrum. It retains the advantages of the CAPON algorithm, which can achieve blind source estimation, and simulations show that the proposed algorithm exhibits a better performance than the traditional algorithm in conditions of various SNRs and snapshot numbers. The simulation results show that, based on a certain SNR, the root mean square error (RMSE) of the improved CAPON algorithm can be reduced from 0.86° to 0.8° compared to traditional algorithms, and the angle estimation error can be decreased by up to about 0.3°. With the help of the cyclic noise reduction method, the angle estimation error decreases from 0.04° to 0.02°.

Off-Grid Underwater Acoustic Source Direction-of-Arrival Estimation Method Based on Iterative Empirical Mode Decomposition Interval Threshold.

To solve the problem that the hydrophone arrays are disturbed by ocean noise when collecting signals in shallow seas, resulting in reduced accuracy and resolution of target orientation estimation, a direction-of-arrival (DOA) estimation algorithm based on iterative EMD interval thresholding (EMD-IIT) and off-grid sparse Bayesian learning is proposed. Firstly, the noisy signal acquired by the hydrophone array is denoised by the EMD-IIT algorithm. Secondly, the singular value decomposition is performed on the denoised signal, and then an off-grid sparse reconstruction model is established. Finally, the maximum a posteriori probability of the target signal is obtained by the Bayesian learning algorithm, and the DOA estimate of the target is derived to achieve the orientation estimation of the target. Simulation analysis and sea trial data results show that the algorithm achieves a resolution probability of 100% at an azimuthal separation of 8° between adjacent signal sources. At a low signal-to-noise ratio of -9 dB, the resolution probability reaches 100%. Compared with the conventional MUSIC-like and OGSBI-SVD algorithms, this algorithm can effectively eliminate noise interference and provides better performance in terms of localization accuracy, algorithm runtime, and algorithm robustness.

A Reduced Complexity Acoustic-Based 3D DoA Estimation with Zero Cyclic Sum.

Accurate direction of arrival (DoA) estimation is paramount in various fields, from surveillance and security to spatial audio processing. This work introduces an innovative approach that refines the DoA estimation process and demonstrates its applicability in diverse and critical domains. We propose a two-stage method that capitalizes on the often-overlooked secondary peaks of the cross-correlation function by introducing a reduced complexity DoA estimation method. In the first stage, a low complexity cost function based on the zero cyclic sum (ZCS) condition is used to allow for an exhaustive search of all combinations of time delays between pairs of microphones, including primary peak and secondary peaks of each cross-correlation. For the second stage, only a subset of the time delay combinations with the lowest ZCS cost function need to be tested using a least-squares (LS) solution, which requires more computational effort. To showcase the versatility and effectiveness of our method, we apply it to the challenging acoustic-based drone DoA estimation scenario using an array of four microphones. Through rigorous experimentation with simulated and actual data, our research underscores the potential of our proposed DoA estimation method as an alternative for handling complex acoustic scenarios. The ZCS method demonstrates an accuracy of 89.4%±2.7%, whereas the ZCS with the LS method exhibits a notably higher accuracy of 94.0%±3.1%, showcasing the superior performance of the latter.

Computationally Efficient Direction Finding for Conformal MIMO Radar.

The use of conformal arrays offers a significant advancement in Multiple-Input-Multiple-Output (MIMO) radar, enabling the placement of antennas on irregular surfaces. For joint Direction-of-Departure (DOD) and Direction-of-Arrival (DOA) estimation in conformal-array MIMO radar, the current spectrum-searching methods are computationally too expensive, while the existing rotation-invariant method may suffer from phase ambiguity caused by the non-Nyquist spacing of the sensors. In this paper, an improved rotationally invariant technique is proposed. The core function of the proposed algorithm is to estimate the phase differences between the adjacent sensors; then, it eliminates phase ambiguity via the previous estimated standard phase difference. Thereafter, DODs and DOAs are obtained via Least Squares (LS) fitting. The proposed method provides closed-form estimates for joint DOD and DOA estimation, which is much more efficient than the existing spectrum-searching techniques. Numerical simulations show that the proposed algorithm can accurately determine 2D DODs and DOAs of targets, only requiring approximately 1% of the running time required by existing spectrum-searching approaches.

Enhanced Coprime Array Structure and DOA Estimation Algorithm for Coherent Sources.

This paper presents a new enhanced coprime array for direction of arrival (DOA) estimation. Coprime arrays are capable of estimating the DOA using coprime properties and outperforming uniform linear arrays. However, the associated algorithms are not directly applicable for estimating the DOA of coherent sources. To overcome this limitation, we propose an enhanced coprime array in this paper. By increasing the number of array sensors in the coprime array, it is feasible to enlarge the aperture of the array and these additional array sensors can be utilized to achieve spatial smoothing, thus enabling estimation of the DOA for coherent sources. Additionally, applying the spatial smoothing technique to the signal subspace, instead of the conventional spatial smoothing method, can further improve the ability to reduce noise interference and enhance the overall estimation result. Finally, DOA estimation is accomplished using the MUSIC algorithm. The simulation results demonstrate improved performance compared to traditional algorithms, confirming its feasibility.

Research and Implementation of Indoor Positioning Algorithm Based on Bluetooth 5.1 AOA and AOD.

With the addition of Bluetooth AOA/AOD direction-finding capabilities in the Bluetooth 5.1 protocol and the introduction of antenna array technology into the Bluetooth platform to further enhance positioning accuracy, Bluetooth has gradually become a research hotspot in the field of indoor positioning due to its standard protocol specifications, rich application ecosystem, and outstanding advantages such as low power consumption and low cost compared to other indoor positioning technologies. However, current indoor positioning based on Bluetooth AOA/AOD suffers from overly simplistic core algorithm implementations. When facing different application scenarios, the standalone AOA or AOD algorithms exhibit weak applicability, and they also encounter challenges such as poor positioning accuracy, insufficient real-time performance, and significant effects of multipath propagation. These existing problems and deficiencies render Bluetooth lacking an efficient implementation solution for indoor positioning. Therefore, this paper proposes a study on Bluetooth AOA and AOD indoor positioning algorithms. Through an analysis of the principles of Bluetooth's newly added direction-finding functionality and combined with research on array signal DOA estimation algorithms, the paper ultimately integrates the least squares algorithm to optimize positioning errors in terms of accuracy and incorporates an anti-multipath interference algorithm to address the impacts of multipath effects in different scenarios. Experimental testing demonstrates that the indoor positioning algorithms applicable to Bluetooth AOA and AOD can effectively mitigate accuracy errors and overcome multipath effects, exhibiting strong applicability and significant improvements in real-time performance.

Low-Complexity 2D-DOD and 2D-DOA Estimation in Bistatic MIMO Radar Systems: A Reduced-Dimension MUSIC Algorithm Approach.

This paper presents a new technique for estimating the two-dimensional direction of departure (2D-DOD) and direction of arrival (2D-DOA) in bistatic uniform planar array Multiple-Input Multiple-Output (MIMO) radar systems. The method is based on the reduced-dimension (RD) MUSIC algorithm, aiming to achieve improved precision and computational efficiency. Primarily, this pioneering approach efficiently transforms the four-dimensional (4D) estimation problem into two-dimensional (2D) searches, thus reducing the computational complexity typically associated with conventional MUSIC algorithms. Then, exploits the spatial diversity of array response vectors to construct a 4D spatial spectrum function, which is crucial in resolving the complex angular parameters of multiple simultaneous targets. Finally, the objective is to simplify the spatial spectrum to a 2D search within a 4D measurement space to achieve an optimal balance between efficiency and accuracy. Simulation results validate the effectiveness of our proposed algorithm compared to several existing approaches, demonstrating its robustness in accurately estimating 2D-DOD and 2D-DOA across various scenarios. The proposed technique shows significant computational savings and high-resolution estimations and maintains high precision, setting a new benchmark for future explorations in the field.

Distance- and Angle-Based Hybrid Localization Integrated in the IEEE 802.15.4 TSCH Communication Protocol.

Accurate localization of devices within Internet of Things (IoT) networks is driven by the emergence of novel applications that require context awareness to improve operational efficiency, resource management, automation, and safety in industry and smart cities. With the Integrated Localization and Communication (ILAC) functionality, IoT devices can simultaneously exchange data and determine their position in space, resulting in maximized resource utilization with reduced deployment and operational costs. Localization capability in challenging scenarios, including harsh environments with complex geometry and obstacles, can be provided with robust, reliable, and energy-efficient communication protocols able to combat impairments caused by interference and multipath, such as the IEEE 802.15.4 Time-Slotted Channel Hopping (TSCH) protocol. This paper presents an enhancement of the TSCH protocol that integrates localization functionality along with communication, improving the protocol's operational capabilities and setting a baseline for monitoring, automation, and interaction within IoT setups in physical environments. A novel approach is proposed to incorporate a hybrid localization by integrating Direction of Arrival (DoA) estimation and Multi-Carrier Phase Difference (MCPD) ranging methods for providing DoA and distance estimates with each transmitted packet. With the proposed enhancement, a single node can determine the location of its neighboring nodes without significantly affecting the reliability of communication and the efficiency of the network. The feasibility and effectiveness of the proposed approach are validated in a real scenario in an office building using low-cost proprietary devices, and the software incorporating the solution is provided. The experimental evaluation results show that a node positioned in the center of the room successfully estimates both the DoA and the distance to each neighboring node. The proposed hybrid localization algorithm demonstrates an accuracy of a few tens of centimeters in a two-dimensional space.

DOA Estimation for Coherent Sources Based on Uniformly Distributed Two Concentric Rings Array.

The direction estimation of the coherent source in a uniform circular array is an essential part of the signal processing area of the array, but the traditional uniform circular array algorithm has a low localization accuracy and a poor localization effect on the coherent source. To solve this problem, this paper proposes a two-dimensional direction of arrival (DOA) estimation for the coherent source in broadband. Firstly, the central frequency of the coherent sound source is estimated using the frequency estimation method of the delayed data, and a real-valued beamformer is constructed using the concept of the multiloop phase mode. Then, the cost function in the beam space is obtained. Finally, the cost function is searched in two dimensions to locate the sound source. In this paper, we simulate the DOA of the sound source at different frequencies and signal-to-noise ratios and analyze the resolution of the circular array. The simulation results show that the proposed algorithm can estimate the direction of arrival with high precision and achieve the desired results.

Efficient Space-Time Signal Processing Scheme of Frequency Synchronization and Positioning for Sensor Networks.

The orthogonal frequency division multiple access (OFDMA) technique has been widely employed in sensor networks as the data modulation scheme. This study presents a one-dimensional (1D) space-time signal processing scheme for the joint estimation of direction of arrival (DOA) and carrier frequency offsets (CFOs) in OFDMA uplink systems. The proposed approach, initiated by a one-dimensional ESPRIT algorithm, involves estimating the DOAs of the received signal to identify subscriber positions. Spatial beamformers are then used to suppress multiple access interference and separate each subscriber's signal from the received signal. The outputs of the spatial beamformer are decimated to estimate the CFO of each subscriber. Compared with conventional two-dimensional parameter estimation algorithms, the proposed one-dimensional algorithm has a higher estimation accuracy and significantly lower computational complexity.

Coherent DOA Estimation Algorithm with Co-Prime Arrays for Low SNR Signals.

The Direction of Arrival (DOA) estimation of coherent signals in co-prime arrays has become a popular research topic. However, traditional spatial smoothing and subspace algorithms fail to perform well under low signal-to-noise ratio (SNR) and small snapshots. To address this issue, we have introduced an Enhanced Spatial Smoothing (ESS) algorithm that utilizes a space-time correlation matrix for de-noising and decoherence. Finally, an Estimating Signal Parameter via Rotational Invariance Techniques (ESPRIT) algorithm is used for DOA estimation. In comparison to other decoherence methods, when the SNR is -8 dB and the number of snapshots is 150, the mean square error (MSE) of the proposed algorithm approaches the Cramér-Rao bound (CRB), the probability of resolution (PoR) can reach over 88%, and, when the angular resolution is greater than 4°, the estimation accuracy can reach over 90%.

Non-Circular Signal DOA Estimation with Nested Array via Off-Grid Sparse Bayesian Learning.

For the traditional uniform linear array (ULA) direction of arrival (DOA) estimation method with a limited array aperture, a non-circular signal off-grid sparse Bayesian DOA estimation method based on nested arrays is proposed. Firstly, the extended matrix of the received data is constructed by taking advantage of the fact that the statistical properties of non-circular signals are not rotationally invariant. Secondly, we use the difference and sum co-arrays for the nested array technique, thus increasing the array aperture and improving the estimation accuracy. Finally, we take the noise as part of the interest signal and iteratively update the grid points using the sparse Bayesian learning (SBL) method to eliminate the modeling errors caused by off-grid gaps. The simulation results show that the proposed algorithm can improve the accuracy of DOA estimation compared with the existing algorithms.

High-Precision DOA Estimation Based on Synthetic Aperture and Sparse Reconstruction.

The direction-of-arrival (DOA) estimation is predominantly influenced by the antenna's aperture size. However, space constraints on flight platforms often necessitate the use of antennas with smaller apertures and fewer array elements. This inevitably imposes limitations on the DOA estimation's resolution and degrees of freedom. To address these precision constraints, we introduce an accurate DOA estimation method based on spatial synthetic aperture model. This method adopts a two-stage strategy to ensure both efficiency and precision in DOA estimation. Initially, the orthogonal matching pursuit (OMP) reconstruction algorithm processes the original aperture data, providing a rough estimate of target angles that guides the aircraft's flight direction. Subsequently, the early estimations merge with the aircraft's motion space samples, forming equivalent spatially synthesized array samples. The refined angle estimation then employs the OMP-RELAX algorithm. Moreover, with the off-grid issue in mind, we devise an estimation method integrating Bayesian parameter estimation with dictionary sequence refinement. The proposed technique harnesses the spatial synthetic aperture for pinpoint estimation, effectively addressing the challenges of atomic orthogonality and angular off-grid on estimation accuracy. Importantly, the efficiency of deploying sparse reconstruction for angle estimation is bolstered by our phased strategy, eliminating the necessity for fine grid analysis across the entire observation scene. Moreover, the poor estimation accuracy caused by coherent source targets and angular-flickering targets is improved by sparse reconstruction. Through simulation and experiment, we affirm the proposed method's efficacy in angle estimation. The results indicate that target angle estimation errors are limited to within 1°. Furthermore, we assess the impact of variables such as target state, heading angle, spatial sampling points, and target distance on the estimation accuracy of our method, showcasing its resilience and adaptability.

Underwater Source Counting with Local-Confidence-Level-Enhanced Density Clustering.

Source counting is the key procedure of autonomous detection for underwater unmanned platforms. A source counting method with local-confidence-level-enhanced density clustering using a single acoustic vector sensor (AVS) is proposed in this paper. The short-time Fourier transforms (STFT) of the sound pressure and vibration velocity measured by the AVS are first calculated, and a data set is established with the direction of arrivals (DOAs) estimated from all of the time-frequency points. Then, the density clustering algorithm is used to classify the DOAs in the data set, with which the number of the clusters and the cluster centers are obtained as the source number and the DOA estimations, respectively. In particular, the local confidence level is adopted to weigh the density of each DOA data point to highlight samples with the dominant sources and downplay those without, so that the differences in densities for the cluster centers and sidelobes are increased. Therefore, the performance of the density clustering algorithm is improved, leading to an improved source counting accuracy. Experimental results reveal that the enhanced source counting method achieves a better source counting performance than that of basic density clustering.

EM and SAGE Algorithms for DOA Estimation in the Presence of Unknown Uniform Noise.

The existing expectation maximization (EM) and space-alternating generalized EM (SAGE) algorithms are only applied to direction of arrival (DOA) estimation in known noise. In this paper, the two algorithms are designed for DOA estimation in unknown uniform noise. Both the deterministic and random signal models are considered. In addition, a new modified EM (MEM) algorithm applicable to the noise assumption is also proposed. Next, these EM-type algorithms are improved to ensure the stability when the powers of sources are not equal. After being improved, simulation results illustrate that the EM algorithm has similar convergence with the MEM algorithm, the SAGE algorithm outperforms the EM and MEM algorithms for the deterministic signal model, and the SAGE algorithm cannot always outperform the EM and MEM algorithms for the random signal model. Furthermore, simulation results show that processing the same snapshots from the random signal model, the SAGE algorithm for the deterministic signal model can require the fewest computations.