Steroidal glycoalkaloids contribute to anthracnose resistance in Solanum lycopersicum.

Anthracnose is a widespread plant disease caused by various species of the fungal pathogen Colletotrichum. In solanaceous plants such as tomato (Solanum lycopersicum), Colletotrichum infections exhibit a quiescent, asymptomatic state in developing fruit, followed by a transition to necrotrophic infections in ripe fruit. Through analysis of fruit tissue extracts of 95L368, a tomato breeding line that yields fruit with enhanced anthracnose resistance, we identified a role for steroidal glycoalkaloids (SGAs) in anthracnose resistance. The SGA α-tomatine and several of its derivatives accumulated at higher levels, in comparison with fruit of the susceptible tomato cultivar US28, and 95L368 fruit extracts displayed fungistatic activity against Colletotrichum. Correspondingly, ripe and unripe 95L368 fruit displayed enhanced expression of glycoalkaloid metabolic enzyme (GAME) genes, which encode key enzymes in SGA biosynthesis. Metabolomics analysis incorporating recombinant inbred lines generated from 95L368 and US28 yielded strong positive correlations between anthracnose resistance and accumulation of α-tomatine and several derivatives. Lastly, transient silencing of expression of the GAME genes GAME31 and GAME5 in anthracnose-susceptible tomato fruit yielded enhancements to anthracnose resistance. Together, our data support a role for SGAs in anthracnose defense in tomato, with a distinct SGA metabolomic profile conferring resistance to virulent Colletotrichum infections in ripe fruit.

Educational Case Studies: Creating a Digital Twin of the Production Line in TIA Portal, Unity, and Game4Automation Framework.

In today's industry, the fourth industrial revolution is underway, characterized by the integration of advanced technologies such as artificial intelligence, the Internet of Things, and big data. One of the key pillars of this revolution is the technology of digital twin, which is rapidly gaining importance in various industries. However, the concept of digital twins is often misunderstood or misused as a buzzword, leading to confusion in its definition and applications. This observation inspired the authors of this paper to create their own demonstration applications that allow the control of both the real and virtual systems through automatic two-way communication and mutual influence in context of digital twins. The paper aims to demonstrate the use of digital twin technology aimed at discrete manufacturing events in two case studies. In order to create the digital twins for these case studies, the authors used technologies as Unity, Game4Automation, Siemens TIA portal, and Fishertechnik models. The first case study involves the creation of a digital twin for a production line model, while the second case study involves the virtual extension of a warehouse stacker using a digital twin. These case studies will form the basis for the creation of pilot courses for Industry 4.0 education and can be further modified for the development of Industry 4.0 educational materials and technical practice. In conclusion, selected technologies are affordable, which makes the presented methodologies and educational studies accessible to a wide range of researchers and solution developers tackling the issue of digital twins, with a focus on discrete manufacturing events.