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Uncontrolled coal mining using non-scientific methods has presented a major threat to the quality of environment, particularly the water resources in eastern himalayan sub-region of India. Water bodies in the vicinity of mining areas are contaminated by acid mine drainage (AMD) that is released into streams and rivers. This study attempted to assess the impact of AMD, deciphering hydrogeochemical processes, seasonal fluctuations, and stable isotope features of water bodies flowing through and around coal mining areas. Self-organizing maps (SOMs) used to separate and categorize AMD, AMD-impacted and non-AMD impacted water from the different study locations for two sampling seasons revealed four clusters (C), with C1 and C2 impacted by AMD, C3 and C4 showing negligible to no impact of AMD. AMD impacted water was SO42- - Mg2+- Ca2+ hydrochemical type with sulphide oxidation and evaporation dominating water chemistry, followed by silicate weathering during both the sampling seasons. Water with negligible-to-no AMD-impact was Mg2+- Ca2+- SO42- to Ca2+ - HCO3- to mixed hydrochemical type with rock weathering and dissolution, followed by ion exchange as major factors controlling water chemistry during both the sampling seasons. Most of physicochemical parameters of C1 and C2 exceeded the prescribed limits, whereas in C3 and C4 water samples, parameters were found within the prescribed limits. Stable isotopes of hydrogen (δ2H) and oxygen (δ18O) during post-monsoon (PoM) varied between -41.04 and -29.98 , and -6.60 to -3.94 ; and during pre-monsoon (PrM) varied between -58.18 and - 33.76 and -8.60 to -5.46 . Deuterium excess (d-excess) ranged between 1.57 and 12.47 during PoM and 5.70 to 15.17 during PrM season. The stable isotopes analysis revealed that evaporation, mineral dissolution and mixing with rainwater are the key factors in study area.
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Minas de Carvão , Monitoramento Ambiental , Isótopos de Oxigênio , Estações do Ano , Índia , Isótopos de Oxigênio/análise , Monitoramento Ambiental/métodos , Poluentes Químicos da Água/análise , Deutério/análise , Rios/químicaRESUMO
Microplastics are ubiquitous, and their widespread prevalence in the ecosphere has generated concerns about their potential effects on terrestrial and aquatic organisms. However, studies pertaining to ecologically sensitive freshwater ecosystems, such as Ramsar wetlands, is scarce. Therefore, the study was conducted in Loktak, the world's only floating lake, and one of the largest wetland in the Indian Himalayan region. The wetland's degradation and pollution have resulted its inclusion in the Montreux Record, underscoring the need for studying this eco-sensitive freshwater system. This work investigated the (i) abundance, morphotype and size of microplastics in fish, and (ii) chemical composition of the microplastics consumed and accumulated in the fish of Loktak lake. Fish samples representing eight species were collected and analyzed for microplastics. Results revealed that â¼91% of the sampled fish ingested microplastics. Fragment was identified as the predominant morphotype (â¼82%). Plastic polymers including polyamide (PA), polystyrene (PS), polycarbonate (PC) and carboxymethyl cellulose (CMC) were detected. The occurrence of heavy metals - chlorine (Cl), palladium (Pd), sodium (Na), zinc (Zn), lead (Pb) and copper (Cu) suggests their adhesion on the microplastics. The occurrence of microplastics in fish indicates pollution in the lake and poses a potential health risks to humans through consumption. Therefore, implementing comprehensive management approaches is imperative to mitigate this emerging pollution and uphold the ecological integrity of the Ramsar site. Substantial information on microplastics and their potential human exposure through fish consumption, particularly in the Indian Himalayan region, remains to be assessed, underscoring the need for extensive study.
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Monitoramento Ambiental , Peixes , Lagos , Microplásticos , Poluentes Químicos da Água , Áreas Alagadas , Animais , Microplásticos/análise , Poluentes Químicos da Água/análise , Índia , Metais Pesados/análise , Plásticos/análiseRESUMO
Global climate change has seriously threatened agriculture and connected sectors, especially in developing countries like India. The Brahmaputra Valley in Assam, Northeast India, is vulnerable to climate change due to its agrarian economy, fragile geo-ecological setting, recurrent floods and droughts, and poor socioeconomic conditions of the farmers. The climate-induced hindrances faced by the rice farming community of this region and the local adaptation practices they employ have not been adequately studied. Therefore, we carried out a survey among 635 rice farmers across four agro-climatic zones of Assam, namely the Upper Brahmaputra Valley Zone, North Bank Plain Zone, Central Brahmaputra Valley Zone, and Lower Brahmaputra Valley Zone, to understand how they perceive and respond to climatic changes. The survey revealed that all the respondents have perceived an increase in ambient temperature, and 65% of the respondents have perceived a slight change in rainfall characteristics over the years. Most farmers reported adjusting the existing farming practices and livelihood choices to adapt to the changing climate. Farming adjustments were made mainly in terms of field preparation and management of water, rice variety, nutrients, and pests. Environmental variables like rainfall, flood, drought, and pest level, and socioeconomic variables like family size, education, farming experience, training, digital media exposure, and land area were found to influence farmers' adaptation choices. The findings imply that policies to strengthen flood, drought, pest management, education, land-use planning, agricultural training, and digital media applications in agriculture are needed for effective climate change adaptation in this region.
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Agricultura , Mudança Climática , Fazendeiros , Oryza , Índia , Agricultura/métodos , Humanos , Inquéritos e Questionários , Secas , Pessoa de Meia-IdadeRESUMO
Mithun (Bos frontalis), a domestically raised herbivore, holds significant economic importance for the farming community of Northeast India. This study aimed to elucidate the genetic parameters governing Mithun body weight traits across different ages using data from the sole organized semi-intensive Mithun farm in India. Information was gathered from 110 Mithuns born over a period spanning from 2011 to 2022. Body weight taken at week 1 (W1), 1-month (M1), 3-months (M3), 6-months (M6), 9-months (M9), 12-months (M12), 30-months (M30) and 45-months (M45) were considered for the study. The genetic parameters estimation employed the BLUPF90 suite of programs, incorporating univariate Gibbs sampler animal model with fixed effects; season and period of birth, and sex of the animal. Variance and covariance components, including direct additive genetic effects, were estimated. Heritability estimates for the eight body weight traits ranged from 0.47 ± 0.0050 to 0.50 ± 0.0043, indicating varying genetic influence across growth stages. Results revealed that Mithun herd has a substantial genetic variability for growth traits and therefore there is ample scope to select for a better growth rate. Here, we conclude that Month 12 (M12) and Month 9 (M9) body weights exhibit higher heritability, indicating potential for genetic improvement through selective breeding.
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Peso Corporal , Animais , Masculino , Feminino , Índia , Modelos Animais , Bovinos/genética , Bovinos/crescimento & desenvolvimento , Bovinos/fisiologia , Variação GenéticaRESUMO
BACKGROUND: The cancer burden in northeast India is high, with low survival and low case detection. Despite the availability of cancer institutes in the region, existing literature remarks on the increasing travel outside the region for cancer care. However, research is sparse on identifying impediment factors to the access of state cancer institutes. OBJECTIVE: To examine the barriers to cancer care in five common cancer sites: oral, lungs, stomach, breast and cervix. METHOD: Following a descriptive multiple-embedded case study design integrating quantitative and qualitative approaches, 388 participants were selected in phase one by stratified random sampling. In phase two, by purposive sampling, 21 semi-structured interviews were conducted. RESULT: The result suggests that family decision is the central factor in cancer care access. Treatment initiation is delayed because the existing government health insurance scheme does not cover diagnostic tests. Adverse steps are taken to fund cancer treatment. Besides, opting for alternative medicines were due to fear of surgery, chemotherapy and recommendations by relatives. Arranging accommodation, transportation and infrastructure shortage was another hurdle. In contrast, the lack of awareness of the state cancer institutes was a barrier to its access. CONCLUSION: This paper identifies and describes factors that hinder access to state cancer institutes. The findings could enhance policy interventions for efficient cancer care access in the region. Integration with NGOs working at the state level for cancer services would support ease of access by providing funds for diagnostic tests, accommodation and transportation, especially for those who cannot afford it.
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Acessibilidade aos Serviços de Saúde , Neoplasias , Feminino , Humanos , Meios de Transporte , Viagem , Índia , Pesquisa Qualitativa , Neoplasias/terapiaRESUMO
INTRODUCTION: Black rice (Oryza sativa L.), which is rich in polyphenols and flavonoids, is indigenous to Northeast India, specifically Manipur, and traditionally consumed for its protective effects on human health. Due to its economic value, it is crucial to evaluate the quality of different black rice varieties to authenticate their therapeutic and nutritional properties. OBJECTIVE: We aimed to evaluate the quality of pre- and post-marketed black rice samples by a validated high-performance thin layer chromatography method and determine variations of total phenolics and total flavonoids with antioxidant potential. MATERIAL AND METHODS: The ferulic acid, gallic acid, quercetin, and caffeic acid contents of three black rice varieties-Poireiton, Amubi, and Sempak-along with two marketed samples of Amubi from Manipur, India, were quantified based on standards. Antioxidant potential was measured by the 2,2-diphenyl-1-picryl-hydrazyl hydrate free radical scavenging assay. RESULTS: The highest and lowest relative biomarker contents were found in hydroalcoholic extracts of Amubi [caffeic acid (1.43% w/w), ferulic acid (1.15% w/w), quercetin (0.6% w/w), and gallic acid (0.39% w/w)] and the marketed sample Var. Amubi from Kakching District, respectively. Pearson's correlation coefficient of antioxidant potential with phenolic and flavonoid content showed a moderate to strong correlation for all samples. CONCLUSION: This validated, rapid, accurate standardization method for black rice varieties will be beneficial for the quality evaluation of black rice and its derived products. It will also be helpful to authenticate the nutritional benefits for the consumers.
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Antioxidantes , Oryza , Humanos , Antioxidantes/análise , Quercetina , Oryza/química , Extratos Vegetais/química , Índia , Flavonoides/análise , Fenóis/análise , Ácido GálicoRESUMO
INTRODUCTION: Ginger constitutes the rhizome part of the plant Zingiber officinale from the Zingiberaceae family. A large number of ginger varieties with high sensorial and functional quality are found in Northeast India. Hence, phytopharmacological screening of different ginger varieties is essential that will serve as a guideline in applied research to develop high-end products and improve economical margins. OBJECTIVE: To determine the variation in total phenolics content (TPC), total flavonoids content (TFC), and antioxidant activities and correlate that with 6-gingerol contents of different ginger varieties collected from Northeast India using Pearson's correlation analysis. MATERIALS AND METHODS: The TPC and TFC values were determined using standard methods. Antioxidant activities were measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical scavenging assays, while reversed-phase high-performance liquid chromatography (RP-HPLC) analysis was utilised for quantitative determination of 6-gingerol content. RESULTS: The result revealed that ginger variety 6 (GV6) contains the highest 6-gingerol content and TPC value showing maximum antioxidant activity, followed by GV5, GV4, GV9, GV3, GV2, GV8, GV1, and GV7. The findings also suggested that the antioxidant activity has much better correlations with TPC as compared with TFC values. Pearson's correlation analysis showed a significant correlation between 6-gingerol contents and TPC values. CONCLUSION: This work underlines the importance of ginger varieties from Northeast India as a source of natural antioxidants with health benefits.
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Antioxidantes , Zingiber officinale , Antioxidantes/química , Flavonoides/análise , Zingiber officinale/química , Catecóis/análise , Catecóis/química , Catecóis/farmacologia , Fenóis/química , Extratos Vegetais/químicaRESUMO
The present study aimed to investigate biodegradability of oily sludge in lab scale composting and slurry bioreactor using a potential bacterial consortium isolated from petroleum-contaminated sites. The consortium used in the study consisted of bacterial genera, including Enterobacter, Bacillus, Microbacterium, Alcaligenes Pseudomonas, Ochrobactrum, Micrococcus, and Shinella which were obtained after rigorous screening using different hydrocarbons. The meticulously designed lab scale composting experiments were carried out and showed that the combination of 10% oily sludge (A1) exhibited the highest total carbon (TC) removal, which was 40.33% within 90 days. To assess the composting experiments' efficiency, the first (k1) and second (k2) order rate constants were evaluated and was found to be 0.0004-0.0067 per day and second (k2) 0.0000008-0.00005 g/kg. day respectively. To further enhance the biodegradation rate of A1 combination, a slurry bioreactor was used. The maximum total petroleum hydrocarbon (TPH) removals in a slurry bioreactor for cycle-I and -II were 48.8% and 46.5%, respectively, on the 78th and 140th days of the treatment. The results obtained in the study will be a technological platform for the development of slurry phase treatment of petroleum waste in a sustainable and eco-friendly manner.
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Compostagem , Petróleo , Poluentes do Solo , Esgotos , Poluentes do Solo/análise , Microbiologia do Solo , Biodegradação Ambiental , Bactérias/metabolismo , Hidrocarbonetos/metabolismo , Reatores BiológicosRESUMO
The Indo-Malayan Realm is a biogeographic realm that extends from the Indian Subcontinent to the islands of Southeast Asia (Malay Archipelago). Despite being megadiverse, evolutionary hypotheses explaining taxonomic diversity in this region have been rare. Here, we investigate the role of geoclimatic events such as Himalayan orogeny and monsoon intensification in the diversification of the ginger-lilies (Hedychium J.Koenig: Zingiberaceae). We first built a comprehensive, time-calibrated phylogeny of Hedychium with 75% taxonomic and geographic sampling. We found that Hedychium is a very young lineage that originated in Northern Indo-Burma, in the Late Miocene (c. 10.6 Ma). This was followed by a late Neogene and early Quaternary diversification, with multiple dispersal events to Southern Indo-Burma, Himalayas, Peninsular India, and the Malay Archipelago. The most speciose clade IV i.e., the predominantly Indo-Burmese clade also showed a higher diversification rate, suggesting its recent rapid radiation. Our divergence dating and GeoHiSSE results demonstrate that the diversification of Hedychium was shaped by both the intensifications in the Himalayan uplift as well as the Asian monsoon. Ancestral character-state reconstructions identified the occurrence of vegetative dormancy in both clades I and II, whereas the strictly epiphytic growth behavior, island dwarfism, lack of dormancy, and a distinct environmental niche were observed only in the predominantly island clade i.e., clade III. Finally, we show that the occurrence of epiphytism in clade III corresponds with submergence due to sea-level changes, suggesting it to be an adaptive trait. Our study highlights the role of recent geoclimatic events and environmental factors in the diversification of plants within the Indo-Malayan Realm and the need for collaborative work to understand biogeographic patterns within this understudied region. This study opens new perspectives for future biogeographic studies in this region and provides a framework to explain the taxonomic hyperdiversity of the Indo-Malayan Realm.
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Zingiber officinale , Zingiberaceae , Sudeste Asiático , Evolução Biológica , Filogenia , Filogeografia , Zingiberaceae/genéticaRESUMO
BACKGROUND: Malaria continues to be a major public health problem in the Northeastern part of India despite the implementation of vector control measures and changes in drug policies. To develop successful vaccines against malaria, it is important to assess the diversity of vaccine candidate antigens in field isolates. This study was done to assess the diversity of Plasmodium falciparum AMA-1 vaccine candidate antigen in a malaria-endemic region of Tripura in Northeast India and compare it with previously reported global isolates with a view to assess the feasibility of developing a universal vaccine based on this antigen. METHODS: Patients with fever and malaria-like illness were screened for malaria and P. falciparum positive cases were recruited for the current study. The diversity of PfAMA-1 vaccine candidate antigen was evaluated by nested PCR and RFLP. A selected number of samples were sequenced using the Sanger technique. RESULTS: Among 56 P. falciparum positive isolates, Pfama-1 was successfully amplified in 75% (n = 42) isolates. Allele frequencies of PfAMA-1 antigen were 16.6% (n = 7) for 3D7 allele and 33.3% (n = 14) in both K1 and HB3 alleles. DNA sequencing revealed 13 haplotypes in the Pfama-1 gene including three unique haplotypes not reported earlier. No unique amino-acid substitutions were found. Global analysis with 2761 sequences revealed 435 haplotypes with a very complex network composition and few clusters. Nucleotide diversity for Tripura (0.02582 ± 0.00160) showed concordance with South-East Asian isolates while recombination parameter (Rm = 8) was lower than previous reports from India. Population genetic structure showed moderate differentiation. CONCLUSIONS: Besides documenting all previously reported allelic forms of the vaccine candidate PfAMA-1 antigen of P. falciparum, new haplotypes not reported earlier, were found in Tripura. Neutrality tests indicate that the Pfama-1 population in Tripura is under balancing selection. This is consistent with global patterns. However, the high haplotype diversity observed in the global Pfama-1 network analysis indicates that designing a universal vaccine based on this antigen may be difficult. This information adds to the existing database of genetic diversity of field isolates of P. falciparum and may be helpful in the development of more effective vaccines against the parasite.
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Antígenos de Protozoários/genética , Malária Falciparum , Plasmodium falciparum , Proteínas de Protozoários/genética , Variação Genética , Haplótipos , Humanos , Índia , Malária Falciparum/epidemiologia , Malária Falciparum/parasitologia , Proteínas de Membrana , Plasmodium falciparum/genética , Polimorfismo de Fragmento de Restrição , Desenvolvimento de VacinasRESUMO
Background: India is a land of diverse food culture and habits and has a plethora of ethnic fermented foods to boast of. Ranging from the north east to the western part of the country and from northern Indian states to the southern tip of the country, fermented ethnic foods have become part of everyday meals and have been able to bridge nutritional gaps and improve general immunity of the people. Most ethnic fermented foods have a rich content of protein, vitamins, fibres and minerals. Aim: Our review attempts to bring forth and summarise the most popular, immune boosting fermented foods of various regions in India, the role microbes' play in their making and how they have impacted the nutritional aspects and immunity of people in various regions of the country. It also highlights the lack of clinical findings in proving the effectiveness of most fermented foods. Methods: Pubmed central and Google scholar were extensively searched from inception to July 2021 for study concepts and topic related keywords. Results: Ethnic fermented food of various Indian regions has shown a definitive role in improving health and immunity. Conclusion: The diverse ethnic fermented foods maybe be meat based, cereal based, pulses based and even vegetable based. Their health benefits and immune boosting abilities are still to be explored to its maximum potential. Often the role of microbes in these fermented foods have also been underplayed and left unexplored.
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Alimentos Fermentados , Bebidas , Fermentação , Humanos , Estado Nutricional , VerdurasRESUMO
BACKGROUND: A detailed analysis of household and individual level Plasmodium infection patterns in two low-endemic districts of Meghalaya was undertaken to better understand the epidemiology of malaria in northeast India. METHODS: Socio-demographic and behavioural information from residents (aged 1-69 years) of households were collected through pre-tested, questionnaire conducted in 2018 and 2019. Blood samples collected from participants were tested for Plasmodium falciparum and/or Plasmodium vivax infection using rapid diagnostic test, microscopy and PCR. Plasma samples from a subset of participants were analysed for antibodies against thirteen P. falciparum and four P. vivax antigens. Associations between household and individual level risk factors, and Plasmodium infections were evaluated using multilevel logistic regression models. RESULTS: A total of 2753 individuals from 827 households were enrolled in 2018, and 834 individuals from 222 households were enrolled in 2019. Of them, 33 (1.2%) were positive by PCR for P. falciparum in 2018 and none were positive for P. vivax. In 2019, no PCR-positive individuals were detected. All, but one, infections were asymptomatic; all 33 infections were sub-microscopic. Reported history of malaria in the past 12 months (OR = 8.84) and history of travel in the past 14 days (OR = 10.06) were significantly associated with Plasmodium infection. A significant trend of increased seropositivity with age was noted for all 17 antigens. Although adults (≥ 18 years) consistently had the highest seropositivity rates, a sizeable proportion of under-five children were also found to be seropositive. Almost all individuals (99.4%) reported sleeping under an insecticide-treated bed-net, and household indoor residual spray coverage in the 12 months preceding the survey was low (23%). Most participants correctly identified common signs and symptoms of malaria, i.e., fever (96.4%), headache (71.2%), chills (83.2%) and body-ache (61.8%). Almost all participants (94.3%) used government-provided services for treatment of malaria. CONCLUSION: This study explored the epidemiology of malaria in two communities in Meghalaya, India, in the context of declining transmission. The presence of widespread asymptomatic infections and seropositivity among under-five children suggest that low-level Plasmodium transmission persists in this region. Implications of the study findings for malaria elimination efforts in low-transmission settings are discussed.
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Malária Falciparum/epidemiologia , Malária Vivax/epidemiologia , Infecções Assintomáticas/epidemiologia , Estudos Transversais , Características da Família , Feminino , Humanos , Incidência , Índia/epidemiologia , Malária Falciparum/prevenção & controle , Malária Vivax/prevenção & controle , Masculino , Plasmodium falciparum/isolamento & purificação , Plasmodium vivax/isolamento & purificação , Fatores de RiscoRESUMO
In the Three-year Report of the Population-Based Cancer Registries: 2012-2014 published by the Indian Council of Medical Research (ICMR 2016) [11], the incidence of nasopharyngeal carcinoma (NPC) in Nagaland has been ranked as the highest in the country and the second-highest globally next to China. With the paucity of literature and very few studies done on the high incidence of NPC among the Nagas in Nagaland, the present study was undertaken to understand and examine the experiences and perceptions of patients diagnosed with NPC and efforts were made to draw on their experiences with cancer care treatment through the thematic analysis of the ten in-depth interviews conducted. The patient's experiences with anxiety, fear, and distress due to NPC, its impact on the reconstruction of self, the coping mechanisms they develop in the form of resilience, personal attributes of hope and optimism, and the impact of religion and prayers in helping them deal with their disease are discussed in the paper.
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Neoplasias Nasofaríngeas , China/epidemiologia , Humanos , Incidência , Narração , Carcinoma Nasofaríngeo/terapia , Neoplasias Nasofaríngeas/epidemiologia , Neoplasias Nasofaríngeas/terapiaRESUMO
The year 2020 has been plagued with COVID-19 and many sectors such as the manufacturing and services are affected, with the educational sector being one of them. Even though a probable way through online learning is found to continue academic activities, the result and the process may not be successful. This study intends to identify the areas of educational disruption due to the COVID-19 situation. For this purpose, a structured questionnaire is used to collect data from students of various part of the Northeast states of India. The findings from the study revealed that there are many reasons that causes educational disruption in the life of students of north-eastern states of India. Students from the north-eastern states of India have been facing poor network, which leads to poor communication between the teachers and students. Continuous lockdown also causes mental stress to the students. As the tension rises due to the on-going pandemic, insecurity regarding the future plans of students also rises. Owing to financial constraints, students are not able to meet the necessary requirements for effective online learning.
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BACKGROUND: Early diagnosis and treatment of malaria symptoms reduces the risk of severe complication and malaria transmission. However, delay in malaria diagnosis and treatment is a major public health problem in India. The primary aim of the study was to determine cut-off for the delay in seeking treatment of fever, and the secondary aim was to identify the factors associated with delay in malaria-endemic areas of Assam, Northeast India. METHODS: The present study analysed data from two prior cross-sectional surveys (community- and hospital-based) that was conducted to study the health-seeking behaviour of people residing in high malaria-endemic areas of Assam, Northeast India. The hospital-based survey data were used to determine optimal cut-off for the delay in reporting, and further, used to identify the factors associated with delay using community-based data. RESULTS: Mean age of fever cases was similar in both community- and hospital-based surveys (23.1 years vs 24.2 years, p = 0.229). Delay in reporting fever was significantly higher among hospital inpatients compared to community-based fever cases (3.6 ± 2.1 vs 4.0 ± 2.6 days; p = 0.006). Delay of > 2 days showed higher predictive ability (sensitivity: 96.4%, and ROC area: 67.5%) compared to other cut-off values (> 3, > 4, and > 5 days). Multivariable logistic regression analysis revealed that the adjusted odds ratio (aOR) of delay was significantly higher for people living in rural areas (1.52, 95%CI: 1.11-2.09), distance (> 5 km) to health facility (1.93, 95%CI: 1.44-2.61), engaged in agriculture work (2.58, 95%CI: 1.97-3.37), and interaction effect of adult male aged 20-40 years (1.71, 95%CI: 1.06-2.75). CONCLUSION: The delay (> 2 days) in seeking treatment was likely to be twice among those who live in rural areas and travel > 5 km to assess health care facility. The findings of the study are useful in designing effective intervention programmes for early treatment of febrile illness to control malaria.
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Diagnóstico Tardio/estatística & dados numéricos , Febre/diagnóstico , Malária/diagnóstico , Aceitação pelo Paciente de Cuidados de Saúde/estatística & dados numéricos , Tempo para o Tratamento/estatística & dados numéricos , Adolescente , Adulto , Estudos Transversais , Feminino , Instalações de Saúde/estatística & dados numéricos , Hospitais/estatística & dados numéricos , Humanos , Índia , Masculino , Pessoa de Meia-Idade , Razão de Chances , Valores de Referência , Estudos Retrospectivos , Inquéritos e Questionários , Adulto JovemRESUMO
BACKGROUND: Lactobacilli are gatekeepers of vaginal ecosystem impeding growth of pathogenic microbes and their diversity varies across populations worldwide. The present study investigated diversity of human vaginal microbiota among females of Northeast India, who are distinct in dietary habits, lifestyle, and genomic composition from rest of India. RESULTS: Altogether, 154 bacterial isolates were obtained from vaginal swab samples of 40 pregnant and 29 non-pregnant females. The samples were sequenced for 16 s rRNA gene and analysed for identification using a dual approach of homology search and maximum likelihood based clustering. Molecular identification based on 16S rRNA gene sequence confirmed the isolates belonging to 31 species. Lactobacilli constituted 37.7% of the bacterial isolates with 10 species and other Lactic Acid Bacteria (39.61%) represented another 10 species, some of which are opportunistic pathogens. The remaining of the communities are mostly dominated by species of Staphylococcus (14.28%) and rarely by Propionibacterium avidum (3.90%), Bacillus subtilis, Escherchia coli, Janthinobacterium lividum, and Kocuria kristinae (each 0.64%). Interestingly Lactobacillus mucosae and Enterococcus faecalis, which are globally uncommon vaginal microbes is found dominant among women of Northeast India. This tentatively reflects adaptability of particular Lactobacillus species, in distinct population, to better compete for receptors and nutrients in vaginal epithelium than other species. Further, intrageneric 16S rRNA gene exchange was observed among Enterococcus, Staphylococcus, and two species of Lactobacillus, and deep intraspecies divergence among L. mucosae, which pinpointed possibility of emergence of new strains with evolved functionality. Lactobacilli percentage decreased from young pregnant to aged non-pregnant women with maximum colonization in trimester II. CONCLUSION: The study highlighted importance of assessment of vaginal microbiota, Lactobacillus in particular, across different population to gain more insight on female health.
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Biodiversidade , Lactobacillus/isolamento & purificação , Vagina/microbiologia , Adolescente , Adulto , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Feminino , Humanos , Índia , Lactobacillus/classificação , Lactobacillus/genética , Microbiota , Filogenia , Gravidez , Adulto JovemRESUMO
We aimed to evaluate the significance of the RANTES-CCR5 axis and resulting immunomodulatory status in Dengue pathogenesis involving a Guwahati, India based population where Dengue cases have increased alarmingly. An increased CC-chemokine receptor type 5 (CCR5) messenger RNA expression and CCR5 positive cell count profile was observed in Dengue cases, the highest being in severe cases. CCR5 ligand RANTES expression was significantly decreased in Dengue cases and inversely correlated with Dengue viremia fold change in severe cases. Monocytes are involved in Dengue virus homing and replication. Its levels and activation profile were higher in Dengue cases. A hyper Th1-biased immunomodulatory profile with upregulated tumor necrosis factor-α levels, and downregulated expression of antiviral cytokine interferon-γ and key regulatory Th2 anti-inflammatory cytokine interleukin 10 was observed in severe Dengue cases compared with mild Dengue cases and controls. The results, therefore, suggest the significance of RANTES-CCR5 axis deregulation and resulting altered immunomodulation in Dengue pathogenesis, and holds prognostic and therapeutic significance.
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Quimiocina CCL5/imunologia , Dengue/imunologia , Imunomodulação , Receptores CCR5/imunologia , Adulto , Quimiocina CCL5/genética , Citocinas/imunologia , Feminino , Humanos , Índia , Interleucina-10/genética , Interleucina-10/imunologia , Ativação Linfocitária , Masculino , Pessoa de Meia-Idade , Monócitos/virologia , Estudos Prospectivos , Receptores CCR5/genética , Dengue Grave/imunologia , Células Th1/imunologia , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/imunologia , Adulto JovemRESUMO
BACKGROUND: Rice (Oryza sativa L.) is one of the most important crops of the world and a major staple food for half of the World's human population. The Northeastern (NE) region of India lies in the Indo-Burma biodiversity hotspot and about 45% of the total flora of the country is found in the region. Local rice cultivars from different states of NE India were analyzed for genetic diversity and population structure using microsatellite markers, and their zinc and iron content. RESULTS: A total of 149 bands were detected using twenty-two microsatellite markers comprising both random and trait-linked markers, showing 100% polymorphism and high value of expected heterozygosity (0.6311) and the polymorphism information content (0.5895). Nali Dhan cultivar of Arunachal Pradesh possessed the highest genetic diversity (0.3545) among studied populations while Moirangphou Khonganbi of Manipur exhibited the lowest genetic diversity (0.0343). The model-based population structure revealed that all the studied 65 rice cultivars were grouped into two clusters. Cluster I was represented by 36 cultivars and cluster II by 29 cultivars. Badalsali cultivar of Assam possessed the highest Zn content (75.8 µg/g) and Kapongla from Manipur possessed the lowest (17.98 µg/g). The highest and the lowest Fe content was found in Fazu (215.62 µg/g) and Idaw (11.42 µg/g) of Mizoram. CONCLUSION: The result suggested rice cultivars of NE India possessing high genetic diversity (Nali dhan), high Zn (Badalsali) and Fe (Fazu) content can be useful as a source of germplasm for future rice improvement programs.
Assuntos
Ferro/metabolismo , Repetições de Microssatélites , Oryza/genética , Zinco/metabolismo , Variação Genética , Índia , Modelos Genéticos , Oryza/classificação , Oryza/metabolismo , FilogeniaRESUMO
Upland rice landraces from different villages of Mizoram, Northeast India were analyzed for seed morphology, amylose content, aromatic characteristic, seed storage protein profiling and genetic diversity. Results revealed variation in grain length, width, weight and shape. Protein profiling showed polypeptide bands ranging from 7 to 10 with similarity coefficient from 0.556 to 1.000 in the studied populations. Population genetic analysis using simple sequence repeats markers revealed a total of 63 alleles with a high level of gene diversity at 0.6468. High values of Fst and PIC estimates were found at 0.7239 and 0.5984 respectively. The Biruchuk population was found to be the most genetically diverse cultivar and least gene diversity was found in Tuikuk buh. The UPGMA trees based on seed morphology, seed storage protein profiling and simple sequence repeats diversity showed the grouping of rice cultivars into three clusters which were further supported by model-based STRUCTURE analysis. This finding is the first-hand report in upland rice of the state and can be useful for selecting suitable rice lines for prebreeding and germplasm conservation of indigenous hill rice cultivars of Mizoram.
Assuntos
Genes de Plantas , Variação Genética , Oryza/genética , Alelos , Amilose/metabolismo , Biomarcadores , Índia , Odorantes , Oryza/embriologia , Oryza/metabolismo , Filogenia , Proteínas de Plantas/metabolismo , Sementes/metabolismoRESUMO
Background: Although inadequate vitamin D and altered parathyroid hormone (PTH) are implicated in deranged glucose metabolism and risk of future diabetes, their role in regulating glucose homeostasis in established cases of diabetes is unclear. We aimed to (i) evaluate vitamin D status, and (ii) determine if vitamin D and PTH were associated with parameters of glucose homeostasis in type 2 diabetes (T2D) patients from Meghalaya, India. Methods: We determined 25-hydroxyvitamin D (25-OH-D) and PTH concentrations in 251 T2D patients (not on insulin), and examined their associations with the following parameters of glucose homeostasis: fasting blood sugar (FBS), post-prandial blood sugar (PPBS), glycated hemoglobin (HbA1c), fasting insulin (FI), homeostasis model assessments of insulin resistance (HOMA-IR) and ß-cell function (HOMA-ß). Results: None of the patients had adequate vitamin D (mean 25-OH-D = 19.3 ng/mL); 47.8% patients were deficient (25-OH-D < 20 ng/mL), while 52.2% were insufficient (25-OH-D < 30 ng/mL) vitamin D. Significant (P < 0.05) univariate associations were observed between: 25-OH-D and FI (r = 0.14); 25-OH-D and HOMA-ß (r = 0.13); PTH and FI (r = -0.18), and PTH and HOMA-ß (r = -0.11). However these associations disappeared after controlling for potential confounders. The 25-OH-D and PTH levels were not associated with any of the tested parameters of glucose homeostasis. Conclusion: There was widespread prevalence of vitamin D deficiency/insufficiency in our sample T2D patients. However, neither vitamin D nor PTH appeared to play a major role in influencing glucose homeostasis in this present selection of T2D cases.