Microalgae colonization of different microplastic polymers in experimental mesocosms across an environmental gradient.

A variety of organisms can colonize microplastic surfaces through biofouling processes. Heterotrophic bacteria tend to be the focus of plastisphere research; however, the presence of epiplastic microalgae within the biofilm has been repeatedly documented. Despite the relevance of biofouling in determining the fate and effects of microplastics in aquatic systems, data about this process are still scarce, especially for freshwater ecosystems. Here, our goal was to evaluate the biomass development and species composition of biofilms on different plastic polymers and to investigate whether plastic substrates exert a strong enough selection to drive species sorting, overcoming other niche-defining factors. We added microplastic pellets of high-density polyethylene (HDPE), polyethylene terephthalate (PET), and a mix of the two polymers in 15 lentic mesocosms in five different locations of the Iberian Peninsula, and after one month, we evaluated species composition and biomass of microalgae developed on plastic surfaces. Our results, based on 45 samples, showed that colonization of plastic surfaces occurred in a range of lentic ecosystems covering a wide geographical gradient and different environmental conditions (e.g., nutrient concentration, conductivity, macrophyte coverage). We highlighted that total biomass differed based on the polymer considered, with higher biomass developed on PET substrate compared to HDPE. Microplastics supported the growth of a rich and diversified community of microalgae (242 species), with some cosmopolite species. However, we did not observe species-specificity in the colonization of the different plastic polymers. Local species pool and nutrient concentration rather than polymeric composition seemed to be the determinant factor defying the community diversity. Regardless of specific environmental conditions, we showed that many species could coexist on the surface of relatively small plastic items, highlighting how microplastics may have considerable carrying capacity, with possible consequences on the wider ecological context.

Macroplastic litter colonization by stream macroinvertebrates relative to that of plant litter: A meta-analysis.

Environmental pollution by anthropogenic litter is a global concern, but studies specifically addressing the interaction between macroplastics and macroinvertebrates in streams are scarce. However, several studies on plant litter decomposition in streams have also used plastic strips as a methodological approach to assess if macroinvertebrates colonize plant litter mostly as a substrate or a food resource. Looking at these studies from the plastic strips perspective may provide useful information on the interaction between macroplastics and macroinvertebrates in streams. I carried out a meta-analysis of 18 studies that have compared macroinvertebrate colonization of macroplastic litter and plant litter in streams to estimate the overall macroinvertebrate colonization of macroplastic litter relative to plant litter, and identify moderators of this difference. Macroinvertebrate colonization of macroplastic litter was overall lower (by âˆ¼ 40%) compared with plant litter. However, differences in macroinvertebrate colonization between macroplastic litter and plant litter were observed when considering leaf litter but not wood litter, which may be a poorer substrate and food resource for macroinvertebrates. Also, differences in macroinvertebrate colonization between macroplastic litter and leaf litter were observed for shredders, collectors and predators, but not for grazers that may feed on the biofilm developed on macroplastics. Macroplastic litter supported lower macroinvertebrate density, biomass, abundance, and richness, but higher macroinvertebrate diversity than leaf litter. Higher macroinvertebrate diversity on macroplastic litter may have occurred when macroplastics represented more heterogeneous substrates (e.g., mixture of plastic types) than leaf litter (e.g., needles). Differences in macroinvertebrate abundance between macroplastic litter and leaf litter were not significantly affected by plastic type, mesh opening size, plant functional group or plant identity. By testing previously untested hypotheses, this meta-analysis guides future empirical studies. Future studies should also consider the geographical areas most affected by macroplastic pollution and the plastic types most often found in the streams.

Epiplastic microhabitats for epibenthic organisms: a new inland water frontier for diatoms.

Plastic pollution is widespread in each type of ecosystems. However, the colonization events of microorganisms on plastics seem to be neglected in inland waters. Therefore, in this study we analyze the possible colonization on the surface (hereafter epiplastic microhabitats) of two typology of plastic supports by diatom community. Specifically, we located 20 supports in expanded polystyrene and 20 in polyethylene terephthalate both floating and dipped (~ 1 m) in a central Italian shallow water pond, in order to evaluate the diachronic colonization of diatoms from November 2019 to August 2020. Our result showed the tendency in colonizing both epiplastic microhabitats without significant differences in number of species; additionally, depth does not appear to affect the number of species. As regard the temporal colonization, the number of species tends to increase over time from autumn-winter to spring-summer in both types of epiplastic microhabitats and depth. Instead, increase in dominance of some species over time has been demonstrated: only a few species keep a high number of individuals compared to the others; therefore, the number of individuals within the species is not uniformly distributed. These results suggest the tendency of diatom community to colonize plastic supports in lentic waters, and this evidence can be very important because artificial supports can increase the surface available for the settlement of the algae community with an increase of productivity and the colonization of new communities of different taxa. Further studies are mandatory to investigate the possible effects on the epiplastic community and the ecological implications in freshwater environments.

New ecological frontiers in the plastisphere: Diatoms and macroinvertebrates turnover assessment by a traits-based approach.

To date, there are very few studies regarding the colonization of artificial substrates in wetlands by macroinvertebrates and diatoms and even fewer are the studies in Italy that take into consideration the diatomic guilds and the biological and ecological traits proposed in literature. Wetlands are at the forefront through the most delicate and threatened freshwater ecosystems. In this study, we want to evaluate the colonization capacity of plastics of diatoms and macroinvertebrates and characterize the diatomic and macroinvertebrate communities using a "traits-based" approach focusing on the colonization of virgin substrates made of polystyrene and polyethylene terephthalate. The study was conducted within the 'Torre Flavia wetland Special Protection Area' a protected wetland area in Central Italy. The study was conducted from November 2019 to August 2020. The results obtained in this study show a tendency of diatom species to colonize artificial plastic supports placed in lentic environments without differences related to the plastic type and water depth. There is also a greater number of species belonging to the "Motile" guild, endowed with a high motility that they exploit to search for more ecologically suitable habitats for settlement. Macroinvertebrates, prefer settlement on polystyrene supports, those on the surface, probably due to the anoxic conditions present on the bottom and the physical structure of the polystyrene that provides shelter to many animal taxa. The analysis on traits highlighted the establishment of an ecologically diverse community mainly formed by univoltine organisms, with dimensions between 5 and 20 mm, predators, choppers and scrapers feeding on plant organisms and animals, but without the formation of a clear ecological system, that is, without evidence of ecological relationships established between two or more taxa. Our research can contribute to underline the ecological complexity of biota inhabiting plastic litter in freshwaters and the implications for plastic-impacted ecosystems biodiversity enrichment.

A new look at the potential role of marine plastic debris as a global vector of toxic benthic algae.

Marine plastic debris provides a significant surface area for potential colonization by planktonic and benthic harmful microalgae and for the adsorption of their toxins. Furthermore, floating plastics may substantially expand the substrate area available for benthic algae in the ocean, intensifying the transfer of potent toxins through pelagic food webs. In this study, we quantify the available surface area of micro- and macroplastics in different oceanic regions and assess the potential role of floating plastics as vectors for the transfer of toxins from three widespread benthic dinoflagellates, Gambierdiscus spp., Ostreopsis cf. ovata and Prorocentrum lima. To avoid bias associated to the occurrence of benthic algae in deep waters, we selected only records from 0 to 100 m depths. We estimate that 26.8 × 1010 cm2 of plastic surface area is potentially available in surface waters of the global ocean, mostly in the size range of large microplastics (1.01-4.75 mm). Based on the distribution of floating plastics and the habitat suitability of the selected microalgal species, the plastic relative colonization risks will be greater in the Mediterranean Sea and in the subtropical and temperate western margins of the oceans, such as the North American and Asian eastern coasts and, to a lesser extent, southern Brazil and Australia. In places where the colonization of O. cf. ovata cells on floating plastic debris has been properly quantified, such as the Mediterranean and southern Brazil, we estimate a colonization potential of up to 2 × 106 cells km-2 of ocean surface during the regular occurrence period and up to 1.7 × 108 cells km-2 during massive blooms of this species. As plastic pollution and harmful benthic algal blooms have both increased substantially over the past decades, we suggest that their interactive effects can become a major and novel threat to marine ecosystems and human health.