Benzoyl Chloride Derivatization Advances the Quantification of Dissolved Polar Metabolites on Coral Reefs.

Extracellular chemical cues constitute much of the language of life among marine organisms, from microbes to mammals. Changes in this chemical pool serve as invisible signals of overall ecosystem health and disruption to this finely tuned equilibrium. In coral reefs, the scope and magnitude of the chemicals involved in maintaining reef equilibria are largely unknown. Processes involving small, polar molecules, which form the majority components of labile dissolved organic carbon, are often poorly captured using traditional techniques. We employed chemical derivatization with mass spectrometry-based targeted exometabolomics to quantify polar dissolved phase metabolites on five coral reefs in the U.S. Virgin Islands. We quantified 45 polar exometabolites, demonstrated their spatial variability, and contextualized these findings in terms of geographic and benthic cover differences. By comparing our results to previously published coral reef exometabolomes, we show the novel quantification of 23 metabolites, including central carbon metabolism compounds (e.g., glutamate) and novel metabolites such as homoserine betaine. We highlight the immense potential of chemical derivatization-based exometabolomics for quantifying labile chemical cues on coral reefs and measuring molecular level responses to environmental stressors. Overall, improving our understanding of the composition and dynamics of reef exometabolites is vital for effective ecosystem monitoring and management strategies.

Children's environmental health and disaster resilience in Puerto Rico and the U.S. Virgin Islands.

The environment plays a significant role in the global burden of disease for children. Climate-related disasters such as the 2017 Atlantic hurricane season are increasingly contributing to this burden. United Nations designated Small Island Developing States (SIDS) like Puerto Rico and the U.S. Virgin Islands are particularly at risk due to environmental health hazards caused by natural disasters, and health care structure vulnerabilities. United Nations' Sustainable Development Goals (UN SDG), specifically UN SDG 3, 13 and 17, focus on climate impacts via promotion of health preparedness and building partnerships between different sectors of society, respectively. The Pediatric Environmental Health Specialty Unit's (PEHSU) work is consistent with these most notably via the delivery of environmental health services along with training nurses, doctors, and other health professionals, formation of partnerships and linking resources. Therefore, training a diverse array of health professionals and linking these groups to relevant community resources is of utmost importance and has the potential to enhance the effective management and early prevention of top environmental health (EH) risks. Nursing is identified as a key health sector to engage for this initiative. This article describes the work of the Federal Region 2 PEHSU in Puerto Rico and the U.S. Virgin Islands that supports health professionals' knowledge building, development of environmental health services, and promotion of wide scale access to such services for children and families. The PEHSU's work is consistent with these most notably with regards to the delivery of environmental health services in pediatrics.

Gene Expression Patterns in Peripheral Blood Leukocytes in Patients with Recurrent Ciguatera Fish Poisoning: Preliminary Studies.

Ciguatera fish poisoning (ciguatera) is a common clinical syndrome in areas where there is dependence on tropical reef fish for food. A subset of patients develops recurrent and, in some instances, chronic symptoms, which may result in substantial disability. To identify possible biomarkers for recurrent/chronic disease, and to explore correlations with immune gene expression, peripheral blood leukocyte gene expression in 10 ciguatera patients (7 recurrent, 3 acute) from the U.S. Virgin Islands, and 5 unexposed Florida controls were evaluated. Significant differences in gene expression were noted when comparing ciguatera patients and controls; however, it was not possible to differentiate between patients with acute and recurrent disease, possibly due to the small sample sizes involved.

Beef Handling Practices Among Consumers in the U.S. Virgin Islands.

U.S. Virgin Islands (USVI) imports more than 95% of its food. Transportation limitations throughout the territory's supply chain can make temperature control of protein foods challenging for consumers. This study aimed to characterize the beef handling practices used by consumers in USVI to determine any educational needs. Printed and online surveys (n = 620 total) were disseminated in USVI through extension agents and local media sources. Three hundred and thirty-four consumers completed a 30-question food handling questionnaire on consumption patterns and food handling from purchasing to their kitchen. Frequencies and Pearson chi-square tests of independence were performed. Beef ranked second among the different meat types consumed, 92% of consumers bought beef from grocery stores, and 55% removed beef from shelves immediately after entering the store. When shopping, 59.1% of respondents always checked the use-by/freeze-by dates of beef, 46.3% always separated beef from other foods, but only 27.5% always used insulated bags. Eighty-three percent of consumers returned home within 1 h of shopping, 92% took less than 30 min to store groceries in either a freezer or refrigerator (98%) and during power outages, 45.1% maintained cold temperatures of beef. Seventy-two percent of consumers washed their hands for more than 10 s, but 33% of those from households with a vulnerable person did not use soap to wash their hands and dried them with reusable towels. When cooking, 44.6% of consumers thawed beef within the temperature danger zone, 80.1% did not check the temperature of beef for doneness, and 34 respondents cooked hamburgers below 160°F. Future consumer food safety education initiatives in the USVI should address hand hygiene among food preparers in homes with vulnerable persons, temperature control practices by promoting the use of insulated bags, safe meat thawing techniques, and the use of thermometers during cooking.

Powerless in the Virgin Islands: Emergency Department and Wound Care Clinic Utilization Following the 2017 Hurricanes Irma and Maria on St. Thomas, U.S. Virgin Islands.

OBJECTIVE: The main objective of this work was to characterize the prevalence of acute medical needs by examining emergency department (ED) and outpatient wound care clinic (WCC) visits before, during, and after the 2017 Hurricanes Irma and Maria, in St. Thomas, United States (U.S.) Virgin Islands. METHODS: Descriptive statistics and logistic regression were used to assess associations between the occurrence of the storms and visits due to injuries and chronic conditions presented to the ED and WCC from September 1, 2016 to May 31, 2018. RESULTS: ED visits increased and the rate of injury care was higher during the storms (12 patient visits per day) than before or after the storms (9 patient visits per day). WCC visits increased during (12%) and after the storms (45%), and were associated with patients 60 years and older. The odds of ED and WCC visits due to injury during the storms were significantly higher (odds ratio [OR]: 1.28, 95% confidence interval [CI]: 1.17, 1.40) than prior to the storms. The odds for visits due to injuries were 1.19 (95% CI: 1.12, 1.28) times higher after the storms. CONCLUSIONS: Increases occurred in ED and WCC visits for injury care during the storms and in WCC visits after the storms. Public health preparedness mandates understanding how major hurricanes impact the prevalence of acute medical needs, and the factors that influence decisions to seek medical care, in their wake.

Rhipicephalus microplus and Dermacentor nitens (Acari: Ixodidae) Coparasitize White-Tailed Deer on St. John, U.S. Virgin Islands.

Ticks parasitizing introduced white-tailed deer, Odocoileus virginianus (Zimmermann), on St. John, U.S. Virgin Islands, were recorded during and after drought conditions. Tick infestation prevalences were 22% at the start of the drought (July 2015), 66% at the height of the drought (March 2016), and 35% after the drought had ended (July 2016; n = 67 deer). Samples of ticks from 22 tranquilized deer in July 2016 revealed the presence of two species, the southern cattle tick, Rhipicephalus (Boophilus) microplus (Canestrini), and the tropical horse tick, Dermacentor (Anocentor) nitens Neumann. Both tick species have considerable veterinary importance, especially for cattle and horses, respectively, as nuisance biters and also as vectors of parasitic piroplasms or of Anaplasma marginale Theiler. All 22 deer examined were infested by R. microplus, whereas 14 (64%) of the samples also included specimens of D. nitens. Because of the large numbers of ticks recorded, wild deer on St. John could develop associated health problems (pruritis, alopecia, anemia, low weight gain, tick-borne pathogens and parasites) and could also serve as a source of these ticks for cattle and horses.

Gene expression patterns in peripheral blood leukocytes in patients with recurrent ciguatera fish poisoning: Preliminary studies.

Ciguatera fish poisoning (ciguatera) is a common clinical syndrome in areas where there is dependence on tropical reef fish for food. A subset of patients develops recurrent and, in some instances, chronic symptoms, which may result in substantial disability. To identify possible biomarkers for recurrent/chronic disease, and to explore correlations with immune gene expression, peripheral blood leukocyte gene expression in 10 ciguatera patients (7 recurrent and 3 acute) from the U.S. Virgin Islands, and 5 unexposed Florida controls were evaluated. Significant differences in gene expression were noted when comparing ciguatera patients and controls; however, it was not possible to differentiate between patients with acute and recurrent disease, possibly due to the small sample sizes involved.

Environmental conditions influence tissue regeneration rates in scleractinian corals.

Natural and anthropogenic factors may influence corals' ability to recover from partial mortality. To examine how environmental conditions affect lesion healing, we assessed several water quality parameters and tissue regeneration rates in corals at six reefs around St. Thomas, US Virgin Islands. We hypothesized that sites closer to developed areas would have poor water quality due to proximity to anthropogenic stresses, which would impede tissue regeneration. We found that water flow and turbidity most strongly influenced lesion recovery rates. The most impacted site, with high turbidity and low flow, recovered almost three times slower than the least impacted site, with low turbidity, high flow, and low levels of anthropogenic disturbance. Our results illustrate that in addition to lesion-specific factors known to affect tissue regeneration, environmental conditions can also control corals' healing rates. Resource managers can use this information to protect low-flow, turbid nearshore reefs by minimizing sources of anthropogenic stress.

Coral reefs and residents of the U.S. Virgin Islands: A relationship of knowledge, outdoor activities and stewardship

To test the hypotheses that U.S. Virgin Islanders’ knowledge about local coral reefs is correlated with behavior, and that different sociological groups of residents have different patterns of knowledge and behavior, a mixed approach to surveying residents was used: (1) personal interviews were held in public locations and (2) an online version of the survey was administered to residents of the U.S. Virgin Islands. From July-October 2008, 462 residents over 18 years old were surveyed. Results indicate that people who engaged in outdoor activities knew significantly more about coral reefs (Spearman p<0.01, r2=0.128). Those more knowledgeable about coral reefs engaged in more positive stewardship activities (e.g. beach clean-ups) (Spearman p<0.01, r2=0.127). Negative behaviors (e.g. anchoring on reef) were not significantly correlated with increased knowledge of coral reefs (Spearman p=0.911, r2=-0.000025). Fishers did not have greater ability in identifying Acropora palmate coral than non-fishers (χ2=4.138, p=0.126); however, swimmers, snorkelers and divers (as a class) were moreable to identify A. palmata than non-swimmers (χ2 =9.764, p=0.002). Most residents identified sea turtle species as endangered (hawksbill turtle, 78.9%) but only 48.2% of the responses included Acropora spp. as threatened. Resident attitudes towards conservation of local resources were overwhelmingly positive. Rev. Biol. Trop. 58 (Suppl. 3): 197-212. Epub 2010 October 01.

Para probar las hipótesis que el conocimiento de los pobladores de las Islas Vírgenes de EEUU con respecto a arrecifes de coral está correlacionado con su conducta, y que los grupos sociológicos de residentes tienen pautas diferentes de conocimiento y de conducta. Se utilizó un enfoque mezclado para analizar a los residentes: (1) entrevistas personales en ubicaciones públicas y (2) una versión en línea de la entrevista fue administrada a residentes de las Islas Vírgenes. De julio a octubre 2008, 462 residentes mayores de 18 años de edad fueron entrevistados. Los resultados indican que personas que practicaban actividades al aire libre sabían mucho más acerca de los arrecifes de coral (Spearman p<0.01, r2=0.128) y las más informadas sobre arrecifes de coral se involucraban en actividades más positivas para su manejo y conservación (Spearman p<0.01, r2=0.127). Las conductas negativas (por ejemplo, anclando en el arrecife) hacia el ambiente no estaban correlacionadas con un mayor conocimiento de los arrecifes de coral (Spearman p=0.911, r2 =-0.000025). Los pescadores no tenían una capacidad mayor de reconocer el coral Acropora palmata que no pescadores (χ2 =4.138, p=0.126); sin embargo, nadadores y buzos fueron más capaces de identificar A. palmata (χ2 =9.764, p=0.002). La mayoría de los residentes saben que las tortugas marinas están en peligro (Tortuga carey, 78.9%) pero solo el 48.2% saben que Acropora está anemazada. La actitutud de los residentes hacia la conservación de los recursos locales es altamente positiva.

New geographic records of Hamlets, Hypoplectrus spp. (Serranidae), in the Caribbean Sea / Nuevos registros geográficos de Hamlets, Hypoplectrus spp. (Serranidae), en el Mar Caribe

The exact number of species of hamlets, Hypoplectrus spp., in the Caribbean is controversial and the geographic distributions of these species/forms are poorly documented. We report Curaçao, Netherlands Antilles, as a new locality for the Barred Hamlet, H. puella (Cuvier), and Shy Hamlet, H. guttavarius (Poey); and St. John and St. Thomas, U.S. Virgin Islands, for the Tan Hamlet, Hypoplectrus sp. The Black Hamlet, H. nigricans (Poey), has previously been reported from Curaçao, but we did not see it there. Rev. Biol. Trop. 54 (Suppl. 3): 171-173. Epub 2007 Jan. 15.

En el Caribe, el número exacto de especies de pez vaca, Hypoplectrus spp., es controversial y la distribución geográfica de estas especies/variedades está pobremente documentada. Presentamos Curaçao, Antillas Holandesas, como nueva localidad para el pez vaca de barras, H. puella (Cuvier) y el vaca bicolor, H. guttavarius (Poey). Agregamos St. John y St. Thomas, Islas Vírgenes de los EUA, al ámbito del "Tan Hamlet", Hypoplectrus sp. Anteriormente se dijo que en Curaçao se halla el pez vaca negra, H. nigricans (Poey), pero no lo vimos en nuestros muestreos.