Single-cell transcriptomic survey of cell diversity and functional changes in yak hearts at different altitude.

The yak (Bos grunniens) is a species adapted to the hypoxic environment in the plateau area. The heart is a hypoxia-sensitive organ involved in this adaptation. Herein, we used single-cell RNA-seq technology and clustering to determine the presence of 11 cell populations in the yak heart. We analyzed gene expression differences and expression patterns in each cell subpopulation at different altitudes. The cells related to altitude changes are mainly smooth muscle cells and vascular endothelial cells. Of the four transcription factors (TFs, MEF2B, FOXP4, ARID5A, and HES4) found in smooth muscle cells, only MEF2B was specifically expressed in vascular smooth muscle cells. Three key TFs (HNF1B, DMRTA1, and ARNTL2) were also found in the cardiomyocyte module. Compared with data extracted from low-altitude yak, we observed that the high altitude yak has enhanced contraction and relaxation of vascular smooth muscle cells and an increased metabolic level of cardiomyocytes. These may be strategies for the yak to adapt to high-altitude hypoxia environments.

Cosmic-ray neutron fluxes and spectra at different altitudes based on Monte Carlo simulations.

Cosmic-ray neutrons (CRNs) account for about half of the radiation dose received by airline crews and passengers at aviation altitudes. CRNs also comprise important background radiation near the ground, which should be considered in neutron counts for the purpose of nuclear safeguarding and homeland security. In this study, simulations were conducted with the Geant4 toolkit to describe the air shower of cosmic rays. The latest experimental driving models for the atmosphere, geomagnetic field, and primary galactic cosmic rays were applied in these simulations. The simulation was validated by comparing the results with those measured on the NASA ER-2 aircraft. the CRN fluxes and spectra were calculated at altitudes ranging from one to tens of kilometers. We determined the characteristics of the CRN spectra and analyzed their dependency on the altitude. To consider their impact on the local environment, the CRNs near the ground were modeled with a specific equivalent approach, which allowed the simulations to be conducted with limited computer processing power. The parameters for the incident primary CRNs near the ground were calculated by simulating the cosmic ray air shower. The modeling dimensions were considered for the air and ground, and an appropriate approximation solution was obtained. The model near the ground was used to investigate the dependences of the CRN flux and spectrum on the soil moisture.

Study on the effect of CaMKKß-mediated AMPK activation on the glycolysis and the quality of different altitude postmortem bovines longissimus muscle.

This study investigated the activity of adenosine monophosphate-activated protein kinase (AMPK), glycolysis, and meat quality index in three altitude bovines during postmortem aging process. Local cattle (altitude:1,500 m), Gannan yak (3,000 m), and Yushu yak (4,500 m) postmortem Longissimus Dorsi (LD) muscle were used. Results indicated that CaCl2 significantly increased the AMPK activity by increasing the calcium-regulated protein kinase kinase (CaMKKß) activity. Besides, AMPK activation enhanced the activity of lactate dehydrogenase (LDH) and Ca2+ -ATPase and accelerated the rate of muscle maturation during postmortem aging. Moreover, the expression of HIF-1, PRKAA2, and GLUT4 genes in high-altitude Yushu yak was higher than that of low-altitude bovines. CaCl2 activates AMPK by activating CaMKKß cascade and accelerates postmortem glycolysis affecting the intramuscular environment, color, and muscle protein degradation to accelerate postmortem muscle maturation, suggesting that AMPK has essential effects on postmortem muscle glycolysis and quality, and can regulate muscle quality by regulating postmortem muscle AMPK activity. PRACTICAL APPLICATIONS: Insufficient postmortem glycolysis usually leads to DFD (dark, firm, and dry) meat. Beef have relatively high incidences of DFD meat, which has an unattractive dark color and causes significant loss to the meat industry. Therefore, AMPK, which can regulate postmortem glycolysis to affect meat quality, is a valid research target.