Life at the conservative end of the leaf economics spectrum: intergeneric variation in the allocation of phosphorus to biochemical fractions in species of Banksia (Proteaceae) and Hakea (Proteaceae).

In severely phosphorus (P)-impoverished environments, plants have evolved to use P very efficiently. Yet, it is unclear how P allocation in leaves contributes to their photosynthetic P-use efficiency (PPUE) and position along the leaf economics spectrum (LES). We address this question in 10 species of Banksia and Hakea, two highly P-efficient Proteaceae genera. We characterised traits in leaves of Banksia and Hakea associated with the LES: leaf mass per area, light-saturated photosynthetic rates, P and nitrogen concentrations, and PPUE. We also determined leaf P partitioning to five biochemical fractions (lipid, nucleic acid, metabolite, inorganic and residual P) and their possible association with the LES. For both genera, PPUE was negatively correlated with fractional allocation of P to lipids, but positively correlated with that to metabolites. For Banksia only, PPUE was negatively correlated with residual P, highlighting a strategy contrasting to that of Hakea. Phosphorus-allocation patterns significantly explained PPUE but were not linked to the resource acquisition vs resource conservation gradient defined by the LES. We conclude that distinct P-allocation patterns enable species from different genera to achieve high PPUE and discuss the implications of different P investments. We surmise that different LES axes representing different ecological strategies coexist in extremely P-impoverished environments.

Cytokinin oxidase/dehydrogenase inhibitors: progress towards agricultural practice.

Cytokinin oxidase/dehydrogenase (CKX) inhibitors reduce the degradation of cytokinins in plants and thereby may improve the efficiency of agriculture and plant tissue culture-based practices. Here, we report a synthesis and structure-activity relationship study of novel urea derivatives concerning their CKX inhibitory activity. The most active compounds showed sub-nanomolar IC50 values with maize ZmCKX1, the lowest value yet documented. Other CKX isoforms of maize and Arabidopsis were also inhibited very effectively. The binding mode of four compounds was characterized based on high-resolution crystal complex structures. Using the soil nematode Caenorhabditis elegans, and human skin fibroblasts, key CKX inhibitors with low toxicity were identified. These compounds enhanced the shoot regeneration of Lobelia, Drosera, and Plectranthus, as well as the growth of Arabidopsis and Brassica napus. At the same time, a key compound (identified as 82) activated a cytokinin primary response gene, ARR5:GUS, and a cytokinin sensor, TCSv2:GUS, without activating the Arabidopsis cytokinin receptors AHK3 and AHK4. This strongly implies that the effect of compound 82 is due to the up-regulation of cytokinin signalling. Overall, this study identifies highly effective and easily prepared CKX inhibitors with a low risk of environmental toxicity for further investigation of their potential in agriculture and biotechnology.

Changes in agricultural nitrogen (N) balance of OECD countries and its causes and impacts.

The Organization for Economic Co-operation and Development (OECD) developed soil surface nutrient balance and made it mandatory for member countries to report annual nutrient budgets since 1990. This study aimed to evaluate the status of nitrogen (N) management in member countries and to figure out why N surplus levels differ across countries and how they relate to other agri-environmental indicators, by analyzing the N budgets from 35 OECD countries over the last 30 years. Of the three factors determining N balance (agricultural land area, N input, and N output), agricultural land area decreased in most OECD countries, negatively affecting N balance reduction. However, OECD's average N balance highly decreased from 91 to 46 kg ha-1 over the last 30 years due to the decrease in N input through inorganic fertilizers and manure, especially in EU countries with high N input levels, while N output did not meaningfully change. In comparison, in Japan and Korea, the N balance slightly increased and they became the highest N balance country recently. A higher N balance led to lower N use efficiency and higher ammonia (NH3) and nitrous oxide (N2O) emission intensities. More densely populated countries with smaller agricultural land per capita (ranging from 0.03 to 0.47 ha capita-1) showed a higher N balance (228-80 kg ha-1), presumably due to higher N input for more agricultural production on limited land. The most densely populated countries among OECD members (Belgium, the Netherlands, Korea, and Japan) had similar N input levels. However, two EU countries had much higher N output than two Asian countries due to higher pasture production, which led to a lower N balance and higher N use efficiency. Therefore, highly populated countries with small arable land areas per capita might need multilateral efforts to alleviate agricultural N balance.

Our national nutrient reduction needs: Applying a conservation prioritization framework to US agricultural lands.

Targeted conservation approaches seek to focus resources on areas where they can deliver the greatest benefits and are recognized as key to reducing nonpoint source nutrients from agricultural landscapes into sensitive receiving waters. Moreover, there is growing recognition of the importance and complementarity of in-field and edge-of-field conservation for reaching nutrient reduction goals. Here we provide a generic prioritization that can help with spatial targeting and applied it across the conterminous US (CONUS). The prioritization begins with identifying areas with high agricultural nutrient surplus, i.e., where the most nitrogen (N) and/or phosphorus (P) inputs are left on the landscape after crop harvest. Subwatersheds with high surplus included 52% and 50% of CONUS subwatersheds for N and P, respectively, and were located predominantly in the Midwest for N, in the South for P, and in California for both N and P. Then we identified the most suitable conservation strategies using a hierarchy of metrics including nutrient use efficiency (proportion of new nutrient inputs removed by crop harvest), tile drainage, existing buffers for agricultural run-off, and wetland restoration potential. In-field nutrient input reduction emerged as a priority because nutrient use efficiency fell below a high but achievable goal of 0.7 (30% of nutrients applied are not utilized) in 45% and 44% of CONUS subwatersheds for N and P, respectively. In many parts of the southern and western US, in-field conservation (i.e., reducing inputs + preventing nutrients from leaving fields) alone was likely the optimal strategy as agriculture was already well-buffered. However, stacking in-field conservation with additional edge-of-field buffering would be important to conservation strategies in 35% and 29% of CONUS subwatersheds for N and P, respectively. Nutrient use efficiencies were often high enough in the Midwest that proposed strategies focused more on preventing nutrients from leaving fields, managing tile effluent, and buffering agricultural fields. Almost all major river basins would benefit from a variety of nutrient reduction conservation strategies, underscoring the potential of targeted approaches to help limit excess nutrients in surface and ground waters.

Coordination of photosynthetic traits across soil and climate gradients.

"Least-cost theory" posits that C3 plants should balance rates of photosynthetic water loss and carboxylation in relation to the relative acquisition and maintenance costs of resources required for these activities. Here we investigated the dependency of photosynthetic traits on climate and soil properties using a new Australia-wide trait dataset spanning 528 species from 67 sites. We tested the hypotheses that plants on relatively cold or dry sites, or on relatively more fertile sites, would typically operate at greater CO2 drawdown (lower ratio of leaf internal to ambient CO2 , Ci :Ca ) during light-saturated photosynthesis, and at higher leaf N per area (Narea ) and higher carboxylation capacity (Vcmax 25 ) for a given rate of stomatal conductance to water vapour, gsw . These results would be indicative of plants having relatively higher water costs than nutrient costs. In general, our hypotheses were supported. Soil total phosphorus (P) concentration and (more weakly) soil pH exerted positive effects on the Narea -gsw and Vcmax 25 -gsw slopes, and negative effects on Ci :Ca . The P effect strengthened when the effect of climate was removed via partial regression. We observed similar trends with increasing soil cation exchange capacity and clay content, which affect soil nutrient availability, and found that soil properties explained similar amounts of variation in the focal traits as climate did. Although climate typically explained more trait variation than soil did, together they explained up to 52% of variation in the slope relationships and soil properties explained up to 30% of the variation in individual traits. Soils influenced photosynthetic traits as well as their coordination. In particular, the influence of soil P likely reflects the Australia's geologically ancient low-relief landscapes with highly leached soils. Least-cost theory provides a valuable framework for understanding trade-offs between resource costs and use in plants, including limiting soil nutrients.

Belowground plant-microbe communications via volatile compounds.

Volatile compounds play important roles in rhizosphere biological communications and interactions. The emission of plant and microbial volatiles is a dynamic phenomenon that is affected by several endogenous and exogenous signals. Diffusion of volatiles can be limited by their adsorption, degradation, and dissolution under specific environmental conditions. Therefore, rhizosphere volatiles need to be investigated on a micro and spatiotemporal scale. Plant and microbial volatiles can expand and specialize the rhizobacterial niche not only by improving the root system architecture such that it serves as a nutrient-rich shelter, but also by inhibiting or promoting the growth, chemotaxis, survival, and robustness of neighboring organisms. Root volatiles play an important role in engineering the belowground microbiome by shaping the microbial community structure and recruiting beneficial microbes. Microbial volatiles are appropriate candidates for improving plant growth and health during environmental challenges and climate change. However, some technical and experimental challenges limit the non-destructive monitoring of volatile emissions in the rhizosphere in real-time. In this review, we attempt to clarify the volatile-mediated intra- and inter-kingdom communications in the rhizosphere, and propose improvements in experimental design for future research.

Progress in research on site-specific nutrient management for smallholder farmers in sub-Saharan Africa.

Increasing fertilizer access and use is an essential component for improving crop production and food security in sub-Saharan Africa (SSA). However, given the heterogeneous nature of smallholder farms, fertilizer application needs to be tailored to specific farming conditions to increase yield, profitability, and nutrient use efficiency. The site-specific nutrient management (SSNM) approach initially developed in the 1990 s for generating field-specific fertilizer recommendations for rice in Asia, has also been introduced to rice, maize and cassava cropping systems in SSA. The SSNM approach has been shown to increase yield, profitability, and nutrient use efficiency. Yield gains of rice and maize with SSNM in SSA were on average 24% and 69% when compared to the farmer practice, respectively, or 11% and 4% when compared to local blanket fertilizer recommendations. However, there is need for more extensive field evaluation to quantify the broader benefits of the SSNM approach in diverse farming systems and environments. Especially for rice, the SSNM approach should be expanded to rainfed systems, which are dominant in SSA and further developed to take into account soil texture and soil water availability. Digital decision support tools such as RiceAdvice and Nutrient Expert can enable wider dissemination of locally relevant SSNM recommendations to reach large numbers of farmers at scale. One of the major limitations of the currently available SSNM decision support tools is the requirement of acquiring a significant amount of farm-specific information needed to formulate SSNM recommendations. The scaling potential of SSNM will be greatly enhanced by integration with other agronomic advisory platforms and seamless integration of digital soil, climate and crop information to improve predictions of SSNM recommendations with reduced need for on-farm data collection. Uncertainty should also be included in future solutions, primarily to also better account for varying prices and economic outcomes.

A comprehensive review on enhancing nutrient use efficiency and productivity of broadacre (arable) crops with the combined utilization of compost and fertilizers.

Broadacre (arable) crops generally require a relatively higher nutrient input toward yield targets. The efficient use of nutrients in arable farmlands is very vital to this endeavor. It minimizes fertilizer input and adverse soil and environmental implications that may arise from the incremental use of fertilizers. It is understood that enhancing the natural capacity of the soil (i.e., the soil's physical, chemical, and biological quality), may effectively improve soil nutrient dynamics, availability, and efficient use by crops. The adoption of integrated nutrient management (INM) approaches such as the organic amendment of the soil in addition to fertilizer use has shown positive impacts on maintaining and recovering soil quality, hence lowering excessive fertilizer use in farmlands. Therefore, this review contextualized the effect of compost and fertilizer on nutrient use efficiency (NUE) and productivity of broadacre crops. The use of compost as an organic soil amendment material has shown some inherently unique advantages and beneficial impacts on soil health and fertility such as improved soil structure, nutrient retention, mobilization, and bioavailability. Several studies have explored these comparative advantages by either blending compost with chemical fertilizer before soil application or a co-application and have noted the observed amelioration of unfavorable soil conditions such as low porosity, high bulk density, low organic matter (OM), unfavorable pH, and cation exchange capacity (CEC), low biological activities with different doses of compost. Consequently, the co-utilization of composts and chemical fertilizers may become viable substitutes for chemical fertilizers in maintaining soil fertility, improving NUE, and crop yield in farmlands. The review further described the comparative environmental and economic implications of adopting the combined utilization of compost and fertilizers in farmlands.

Potassium and phosphorus content ratio in hydroponic culture affects tomato plant growth and nutrient uptake.

Mineral nutrient deficiencies induce a cascade of physiological, morphological, and biochemical changes in plants which reduce vegetative growth. In this work, the impact of P and K concentration levels on tomato plant development grown in hydroponic culture was investigated. Root morphology, chlorophyll a fluorescence, phosphorus (P) and potassium (K) content, and shoot and root biomass were analyzed. Root morphology showed significant differences among the plants grown in hydroponic culture with different concentrations of P and K. Plant root/shoot dry biomass ratio decreased by 22 and 35% for P15K0 and P30K0, respectively, compared to the control (P30K232). The deficiency of P and K (individually or both) reduced significantly the root mass density parameter. For example, root mass density decreased by 38% at P15K0 treatment compared to control. Correlation analysis showed that the P and K content ratio in shoot and root was significantly and positively correlated with root volume. Deficiencies in K and P decreased the relative size of the PSI final electron acceptor pool and the electron flow on the acceptor side of PSI. Tomato growth response depend on the availability of P and K, however, interactions between these two nutrients could influence their uptake and utilization.

Invasion-induced root-fungal disruptions alter plant water and nitrogen economies.

Despite widespread evidence that biological invasion influences both the biotic and abiotic soil environments, the extent to which these two pathways underpin the effects of invasion on plant traits and performance remains unknown. Leveraging a long-term (14-year) field experiment, we show that an allelochemical-producing invader affects plants through biotic mechanisms, altering the soil fungal community composition, with no apparent shifts in soil nutrient availability. Changes in belowground fungal communities resulted in high costs of nutrient uptake for native perennials and a shift in plant traits linked to their water and nutrient use efficiencies. Some plants in the invaded community compensate for the disruption of nutritional symbionts and reduced nutrient provisioning by sanctioning more nitrogen to photosynthesis and expending more water, which demonstrates a trade-off in trait investment. For the first time, we show that the disruption of belowground nutritional symbionts can drive plants towards alternative regions of their trait space in order to maintain water and nutrient economics.

Response of root development and nutrient uptake of two chinese cultivars of hybrid rice to nitrogen and phosphorus fertilization in Sichuan Province, China.

BACKGROUND: Chemical fertilization helped modern agriculture in grain yield improvement to ensure food security. The response of chemical fertilization for higher hybrid rice production is highly dependent on optimal fertilization management in paddy fields. To assess such responses, in the current work we examine the yield, root growth, and expression of related genes responsible for stress metabolism of nitrogen (N) and phosphorus (P) in two hybrid-rice cultivars Deyou4727 (D47) and Yixiangyou2115 (Y21). METHODS AND RESULTS: The experiment followed four nitrogen (N) (N0, N60, N120, and N180 kg/ha) and phosphorus (P) (P0, P60, P90, and P120 kg/ha) fertilizer levels. The grain yield in D47 was more sensitive to nitrogen application, while Y21 was more sensitive to phosphorus application, which resulted in comparatively higher biomass and yield. Our findings were corroborated by gene expression studies of glutamine synthetase OsGS1;1 and OsGS1;2 and phosphate starvation-related genes PHR1 and SPX, confirming sensitivity to N and P application. The number of roots was less sensitive to nitrogen application in D47 between N0 and N60, but the overall nutrient response difference was significantly higher due to the deep rooting system as compared to Y21. CONCLUSIONS: The higher yield, high N and P use efficiency, and versatile root growth of D47 make it suitable to reduce unproductive usage of N and P from paddy fields, improving hybrid rice productivity, and environmental safety in the Sichuan basin area of China.

Potassium ferrite nanoparticles on DAP to formulate slow release fertilizer with auxiliary nutrients.

Low use efficiency of nitrogen (N) and phosphorus (P) is major challenge of modern agriculture. Coating of conventional fertilizers with nanomaterials is a promising technique for improved nutrient use efficiency. In current study, nanoparticles (NPs) of potassium ferrite (KFeO2 NPs) were coated on di-ammonium phosphate (DAP) fertilizer with three rates (2, 5, 10%) of KFeO2 NPs and were evaluated for release of N, P, K and Fe supplementation in clay loam and loam soil up to 60 days. The NPs were characterized for crystal assemblage, bond formation, morphology and configuration using the x-ray diffraction (XRD), scanning electron microscope (SEM) and Fourier transform-infra red spectroscopy (FT-IR). The results showed that size of NPs ranged between 7 and 18 nm. The controlled release of P in 10% KFeO2 nano-coated DAP was observed throughout the incubation period. The P release kept on increasing from day-1 (14.5 µg g-1) to day-60 (178.6 µg g-1) in coated DAP (10%) in loam soil. The maximum release of 50.4 µg g-1 NH4+1-N in coated DAP (10%) was observed after 30 days of incubation. The release of NO3-1-N was consistent up to 45 and 60 days in clay loam and loam soil, respectively. The average release of potassium and iron in 60 days was 19.7 µg g-1 and 7.3 µg g-1 higher in 10% coated DAP than traditional DAP in clay loam soil. It was concluded that KFeO2 nano-coated DAP supplied P and mineral N for longer period of time in both soils, and some higher coating levels should be tested in future.

Innovative high digestibility protein feed materials reducing environmental impact through improved nitrogen-use efficiency in sustainable agriculture.

Sustainable development in agriculture brings both environmental and economic benefits. Contemporary agriculture is also about increasing nutrient use efficiency, especially nitrogen, as the critical nutrient causing the most significant environmental pressure. This creates the need to produce highly digestible protein feed with high bioavailability, reducing losses of biogenic elements to feces. In this review, the latest trends and the potential for their implementation in sustainable agriculture have been compared, as well as the need to reduce the negative environmental impact of agriculture has been demonstrated. Applying local protein sources to feed animals reduces greenhouse gas emissions associated with transportation. The production of highly digestible fodder leads to a reduction in environmental pollution caused by excessive nitrogen outflows. Another approach indecreasing ammonia emissions from livestock farming is feed protein reduction and amino acid supplementation. All of the aforementioned approaches may result in beneficial long-term changes, contributing to environmental safety, animal welfare and human health.

Can drip irrigation under mulch be replaced with shallow-buried drip irrigation in spring maize production systems in semiarid areas of northern China?

BACKGROUND: In recent years, shallow-buried drip irrigation in spring maize production has gradually replaced drip irrigation under plastic films and has become a common irrigation method in semiarid areas of northern China. RESULT: Two years of field experiments were carried out in the semiarid area of western Jilin province to compare the two drip irrigation methods in the spring maize production system. The treatments included MW1 (drip irrigation under mulch + moderate irrigation amount), MW2 (drip irrigation under mulch + high irrigation amount), SM1 (shallow-buried drip irrigation + moderate irrigation amount), and SM2 (shallow-buried drip irrigation + high irrigation amount). The maize yields were significantly higher under mulch than under shallow-buried drip irrigation, but there was no interaction between mulch and the irrigation amount. Drip irrigation under mulch greatly improved spring maize N, P and K uptake compared with that under shallow-buried drip irrigation. The agronomic-use efficiency, recovery-use efficiency, and partial factor productivity were ranked in the order of MW2 > MW1 > SW2 > SW1. The water-use efficiency of drip irrigation under mulch was 7.44% and 6.82% higher than that of shallow-buried drip irrigation under the moderate and high irrigation levels, respectively. However, considering the costs of the plastic mulch, there was no significant (P < 0.05) difference in economic benefits between the SW and MW treatments. CONCLUSION: Drip irrigation under plastic film provides greater advantages for production, but shallow-buried drip irrigation may be a suitable method for farmers until fertigation technology is further optimized and the problem of plastic film pollution is solved. © 2020 Society of Chemical Industry.

Neighbouring plants modify maize root foraging for phosphorus: coupling nutrients and neighbours for improved nutrient-use efficiency.

Nutrient distribution and neighbours can impact plant growth, but how neighbours shape root-foraging strategy for nutrients is unclear. Here, we explore new patterns of plant foraging for nutrients as affected by neighbours to improve nutrient acquisition. Maize (Zea mays) was grown alone (maize), or with maize (maize/maize) or faba bean (Vicia faba) (maize/faba bean) as a neighbour on one side and with or without a phosphorus (P)-rich zone on the other in a rhizo-box experiment. Maize demonstrated root avoidance in maize/maize, with reduced root growth in 'shared' soil, and increased growth away from its neighbours. Conversely, maize proliferated roots in the proximity of neighbouring faba bean roots that had greater P availability in the rhizosphere (as a result of citrate and acid phosphatase exudation) compared with maize roots. Maize proliferated more roots, but spent less time to reach, and grow out of, the P patches away from neighbours in the maize/maize than in the maize/faba bean experiment. Maize shoot biomass and P uptake were greater in the heterogeneous P treatment with maize/faba bean than with maize/maize system. The foraging strategy of maize roots is an integrated function of heterogeneous distribution of nutrients and neighbouring plants, thus improving nutrient acquisition and maize growth. Understanding the foraging patterns is critical for optimizing nutrient management in crops.

Assessing the variation in manganese use efficiency traits in Scottish barley landrace Bere (Hordeum vulgare L.).

BACKGROUND AND AIMS: Manganese (Mn) deficiency in barley is a global problem. It is difficult to detect in the early stages of symptom development and is commonly pre-emptively corrected by Mn foliar sprays that can be costly. Landraces adapted to marginal lands around the world represent a genetic resource for potential sustainability traits including mineral use efficiency. This research aims to confirm novel Mn use efficiency traits from the Scottish landrace Bere and use an association mapping approach to identify genetic loci associated with the trait. METHODS: A hydroponic system was developed to identify and characterize the Mn deficiency tolerance traits in a collection of landraces, including a large number of Scottish Bere barleys, a group of six-rowed heritage landraces grown in the highlands and islands of Scotland. Measuring chlorophyll fluorescence, the effect of Mn deficiency was identified in the early stages of development. Genotypic data, generated using the 50k Illumina iSelect genotyping array, were coupled with the Mn phenotypic data to create a genome-wide association study (GWAS) identifying candidate loci associated with Mn use efficiency. KEY RESULTS: The Bere lines generally had good Mn use efficiency traits. Individual Bere lines showed large efficiencies, with some Bere lines recording almost double chlorophyll fluorescence readings in limited Mn conditions compared with the elite cultivar Scholar. The Mn-efficient Bere lines had increased accumulation of Mn in their shoot biomass compared with elite cultivars, which was highly correlated to the chlorophyll fluorescence. Several candidate genes were identified as being associated with Mn use efficiency in the GWAS. CONCLUSIONS: Several genomic regions for Mn use efficiency traits originating from the Bere lines were identified. Further examination and validation of these regions should be undertaken to identify candidate genes for future breeding for marginal lands.

Science-based decision support for formulating crop fertilizer recommendations in sub-Saharan Africa.

In sub-Saharan Africa, there is considerable spatial and temporal variability in relations between nutrient application and crop yield, due to varying inherent soil nutrients supply, soil moisture, crop management and germplasm. This variability affects fertilizer use efficiency and crop productivity. Therefore, development of decision systems that support formulation and delivery of site-specific fertilizer recommendations is important for increased crop yield and environmental protection. Nutrient Expert (NE) is a computer-based decision support system, which enables extension advisers to generate field- or area-specific fertilizer recommendations based on yield response to fertilizer and nutrient use efficiency. We calibrated NE for major maize agroecological zones in Nigeria, Ethiopia and Tanzania, with data generated from 735 on-farm nutrient omission trials conducted between 2015 and 2017. Between 2016 and 2018, 368 NE performance trials were conducted across the three countries in which recommendations generated with NE were evaluated relative to soil-test based recommendations, the current blanket fertilizer recommendations and a control with no fertilizer applied. Although maize yield response to fertilizer differed with geographic location; on average, maize yield response to nitrogen (N), phosphorus (P) and potassium (K) were respectively 2.4, 1.6 and 0.2 t ha-1 in Nigeria, 2.3, 0.9 and 0.2 t ha-1 in Ethiopia, and 1.5, 0.8 and 0.2 t ha-1 in Tanzania. Secondary and micronutrients increased maize yield only in specific areas in each country. Agronomic use efficiencies of N were 18, 22 and 13 kg grain kg-1 N, on average, in Nigeria, Ethiopia and Tanzania, respectively. In Nigeria, NE recommended lower amounts of P by 9 and 11 kg ha-1 and K by 24 and 38 kg ha-1 than soil-test based and regional fertilizer recommendations, respectively. Yet maize yield (4 t ha-1) was similar among the three methods. Agronomic use efficiencies of P and K (300 and 250 kg kg-1, respectively) were higher with NE than with the blanket recommendation (150 and 70 kg kg-1). In Ethiopia, NE and soil-test based respectively recommended lower amounts of P by 8 and 19 kg ha-1 than the blanket recommendations, but maize yield (6 t ha-1) was similar among the three methods. Overall, fertilizer recommendations generated with NE maintained high maize yield, but at a lower fertilizer input cost than conventional methods. NE was effective as a simple and cost-effective decision support tool for fine-tuning fertilizer recommendations to farm-specific conditions and offers an alternative to soil testing, which is hardly available to most smallholder farmers.

Impacts of intensifying or expanding cereal cropping in sub-Saharan Africa on greenhouse gas emissions and food security.

Cropping is responsible for substantial emissions of greenhouse gasses (GHGs) worldwide through the use of fertilizers and through expansion of agricultural land and associated carbon losses. Especially in sub-Saharan Africa (SSA), GHG emissions from these processes might increase steeply in coming decades, due to tripling demand for food until 2050 to match the steep population growth. This study assesses the impact of achieving cereal self-sufficiency by the year 2050 for 10 SSA countries on GHG emissions related to different scenarios of increasing cereal production, ranging from intensifying production to agricultural area expansion. We also assessed different nutrient management variants in the intensification. Our analysis revealed that irrespective of intensification or extensification, GHG emissions of the 10 countries jointly are at least 50% higher in 2050 than in 2015. Intensification will come, depending on the nutrient use efficiency achieved, with large increases in nutrient inputs and associated GHG emissions. However, matching food demand through conversion of forest and grasslands to cereal area likely results in much higher GHG emissions. Moreover, many countries lack enough suitable land for cereal expansion to match food demand. In addition, we analysed the uncertainty in our GHG estimates and found that it is caused primarily by uncertainty in the IPCC Tier 1 coefficient for direct N2 O emissions, and by the agronomic nitrogen use efficiency (N-AE). In conclusion, intensification scenarios are clearly superior to expansion scenarios in terms of climate change mitigation, but only if current N-AE is increased to levels commonly achieved in, for example, the United States, and which have been demonstrated to be feasible in some locations in SSA. As such, intensifying cereal production with good agronomy and nutrient management is essential to moderate inevitable increases in GHG emissions. Sustainably increasing crop production in SSA is therefore a daunting challenge in the coming decades.

Arbuscular mycorrhiza: a viable strategy for soil nutrient loss reduction.

Arbuscular mycorrhiza fungi's (AMF) role in plant nutrition and stress management is well known, but very few researches and studies have been conducted so far on the fungal ability to reduce different nutrient losses (runoff, leaching and volatilization) from the soil system. This important ecosystem service of AMF had been neglected largely. From the recent findings, it has been confirmed that mycorrhizal symbiosis has potential to check the losses of applied nutrients. The role of soil biota in nutrient cycling is indispensable and determines the nutrient availability to plants. Among these biota, AMF's association with plants is the most prevalent, but the exact mechanisms followed by AMF in nutrient cycling, transformation and reducing nutrient loss ability are still inconclusive. In this review, we will try to unlock this particular aspect of AMF which is important to achieve global food demand in a sustainable way.

Ancient barley landraces adapted to marginal soils demonstrate exceptional tolerance to manganese limitation.

BACKGROUND AND AIMS: Micronutrient deficiency in cereals is a problem of global significance, severely reducing grain yield and quality in marginal soils. Ancient landraces represent, through hundreds of years of local adaptation to adverse soil conditions, a unique reservoir of genes and unexplored traits for enhancing yield and abiotic stress tolerance. Here we explored and compared the genetic variation in a population of Northern European barley landraces and modern elite varieties, and their tolerance to manganese (Mn) limitation. METHODS: A total of 135 barley accessions were genotyped and the genetic diversity was explored using Neighbor-Joining clustering. Based on this analysis, a sub-population of genetically diverse landraces and modern elite control lines were evaluated phenotypically for their ability to cope with Mn-deficient conditions, across three different environments increasing in complexity from hydroponics through pot experiments to regional field trials. KEY RESULTS: Genetically a group of Scottish barley landraces (Bere barley) were found to cluster according to their island of origin, and accessions adapted to distinct biogeographical zones with reduced soil fertility had particularly larger Mn, but also zinc (Zn) and copper (Cu) concentrations in the shoot. Strikingly, when grown in an alkaline sandy soil in the field, the locally adapted landraces demonstrated an exceptional ability to acquire and translocate Mn to developing leaves, maintain photosynthesis and generate robust grain yields, whereas modern elite varieties totally failed to complete their life cycle. CONCLUSIONS: Our results highlight the importance of gene pools of local adaptation and the value of ancient landrace material to identify and characterize genes that control nutrient use efficiency traits in adverse environments to raise future crop production and improve agricultural sustainability in marginal soils. We propose and discuss a model summarizing the physiological mechanisms involved in the complex trait of tolerance to Mn limitation.