Lower tear meniscus height measured by optical coherence tomography in children.

INTRODUCTION: While optical coherence tomography (OCT) measurements of the lower tear meniscus height (LTMH) have been reported in adults, here we obtained LTMH measurements through Fourier Domain OCT in healthy children and compared these with values obtained in healthy adults. METHODS: Participants were children 7-17 years of age and a control group of adults 20-40 years of age. Inclusion criteria were no abnormal eye conditions or the use of contact lenses. Candidates who fulfilled the TFOS DEWS II criteria for dry eye disease (DED) were excluded. All subjects underwent LTMH measurement (OCT Spectralis) and tests for non-invasive tear break-up time and ocular surface staining. Participants also completed the ocular surface disease index questionnaire. RESULTS: A total of 86 children and 27 adults were included. Mean LTMH values in the children and adult groups were 217.40 ± 71.40 µm and 225.0 ± 54.86 µm, respectively; p = 0.53. However, 59.3% of the children had an LTMH ≤210 µm suggestive of DED, compared with only 33.3% of adults (p = 0.02). For the children, no significant differences in LTMH were observed with sex or for those more or less than 12 years of age. CONCLUSIONS: Optical coherence tomography-derived LTMH measurements were obtained in healthy children. While values were similar in children and adults, a greater proportion of children had an LTMH compatible with a diagnosis of DED. More studies in different paediatric populations are required to establish a complete set of normative LTMH measurements.

Evaluation of tear meniscus height using different clinical methods.

Clinical relevance: The height of the tear meniscus (TMH) is a generally accepted method to evaluate tear film volume, especially in dry eye diagnoses and management.Background: The purpose of this study was to evaluate the ability of different methods to measure tear meniscus height accurately and repeatably.Methods: Lower TMH of 20 volunteers (26.8 ± 5.6 years) was measured by two observers (OI and OII) using optical coherence tomography (OCT), slitlamp microscope image analysis, and with a reticule at low (8x) and high (32x) magnification. TMH was also evaluated by both observers by comparing TMH to thickness of the lid margin (lid-ratio; grade 0: TMH 1/2 lid margin thickness; grade 1: 1/3; grade 2: 1/4; grade 3: 1/5; grade 4: 1/6) and to the number of eyelashes fitting in the tear meniscus. Differences between observers were analysed by paired-t-test. Differences between OCT-TMH and other methods were analysed by ANOVA, and inter-observer repeatability by intra-class-correlation-coefficient (ICC). The ability to predict OCT-TMH was calculated by receiver operative characteristic (ROC) curve analysis.Results: There was no significant difference between OI and OII in all methods except of the eyelash-count-method (p = 0.008). For OI, TMH measured using a reticule at 8x (0.20 ± 0.05 mm) was significantly lower than OCT-TMH (0.24 ± 0.07 mm) (p = 0.032) but not at 32x (0.22 ± 0.01 mm; p = 0.435). TMH evaluated by the image software of the slitlamp (0.20 ± 0.05 mm) was significantly lower than OCT-TMH (p = 0.022). The lid-ratio-method and eyelash-count-method resulted in grades of 2.35 ± 1.22 and 2.85 ± 0.81, respectively. ROC analyses showed that only the 8x and the 32x magnification method could discriminate between normal and abnormal OCT-TMH. OCT had the best repeatability (ICC = 0.88; p < 0.001) followed by reticule using 32x magnification (ICC = 0.70; p = 0.004).Conclusion: The most reliable method to measure TMH was OCT followed by slitlamp using a reticule. TMH cannot be reliably evaluated by comparing it against lid margin thickness or number of eyelashes.