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1.
Biotechnol Bioeng ; 121(9): 2752-2766, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-38877732

RESUMO

Natural hydrogels are widely employed in tissue engineering and have excellent biodegradability and biocompatibility. Unfortunately, the utilization of such hydrogels in the field of three-dimensional (3D) printing nasal cartilage is constrained by their subpar mechanical characteristics. In this study, we provide a multicrosslinked network hybrid ink made of photocurable gelatin, hyaluronic acid, and acrylamide (AM). The ink may be processed into intricate 3D hydrogel structures with good biocompatibility and high stiffness properties using 3D printing technology based on digital light processing (DLP), including intricate shapes resembling noses. By varying the AM content, the mechanical behavior and biocompatibility of the hydrogels can be adjusted. In comparison to the gelatin methacryloyl (GelMA)/hyaluronic acid methacryloyl (HAMA) hydrogel, adding AM considerably enhances the hydrogel's mechanical properties while also enhancing printing quality. Meanwhile, the biocompatibility of the multicrosslinked network hydrogels and the development of cartilage were assessed using neonatal Sprague-Dawley (SD) rat chondrocytes (CChons). Cells sown on the hydrogels considerably multiplied after 7 days of culture and kept up the expression of particular proteins. Together, our findings point to GelMA/HAMA/polyacrylamide (PAM) hydrogel as a potential material for nasal cartilage restoration. The photocuring multicrosslinked network ink composed of appropriate proportions of GelMA/HAMA/PAM is very suitable for DLP 3D printing and will play an important role in the construction of nasal cartilage, ear cartilage, articular cartilage, and other tissues and organs in the future. Notably, previous studies have not explored the application of 3D-printed GelMA/HAMA/PAM hydrogels for nasal cartilage regeneration.


Assuntos
Hidrogéis , Cartilagens Nasais , Impressão Tridimensional , Ratos Sprague-Dawley , Alicerces Teciduais , Animais , Ratos , Hidrogéis/química , Alicerces Teciduais/química , Condrócitos/citologia , Engenharia Tecidual , Ácido Hialurônico/química , Gelatina/química , Bioimpressão/métodos
2.
Am J Otolaryngol ; 45(4): 104307, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38678803

RESUMO

OBJECTIVE: The aim of this study is to demonstrate use of Lateral Alar Caudal Graft to increase nasal tip definition in primary or revision rhinoplasty cases. METHOD: Lateral Alar Caudal Graft was used in 24 patients who underwent open technique septorhinoplasty between 2019 and 2023 years. Fourteen of the patients were female and 10 were male. This technique was applied in revision or primary cases where the caudal part of the lateral alar crura of the lower lateral cartilage was lower in the vertical plane than the cephalic part. The Lateral Alar Caudal Graft was sutured over the caudal edge of the lateral alar crura of the lower lateral cartilage. RESULT: The mean age of the patients was 23. The mean follow-up time of the patients was between 6 and 18 months. No complications were observed due to this technique. Satisfactory results were obtained in the postoperative period after surgery. CONCLUSION: We have successfully demonstrated that in primary or revision rhinoplasty cases, nasal tip definition will be enhanced by positioning the caudal level of the lateral alar crura of the lower lateral cartilage higher than the cephalic level using an onlay lateral alar crural graft and eliminated the need for alar rim graft.


Assuntos
Cartilagens Nasais , Rinoplastia , Humanos , Rinoplastia/métodos , Masculino , Feminino , Adulto , Adulto Jovem , Cartilagens Nasais/transplante , Cartilagens Nasais/cirurgia , Resultado do Tratamento , Reoperação/métodos , Septo Nasal/cirurgia , Seguimentos , Nariz/cirurgia
3.
Am J Otolaryngol ; 45(5): 104434, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39059162

RESUMO

BACKGROUND: In rhinoplasty, it is very important to adjust the rotation and projection of the tip together harmoniously with the nasal dorsum and face to achieve pleasing results. OBJECTIVE: In this study, our aim is to describe a new modification of the vertical alar resection technique that can effectively regulate nasal tip projection and rotation. MATERIALS AND METHODS: Versatile vertical alar resection (V-VAR) technique was applied to 14 primary and 9 revision rhinoplasty cases with highly projected nasal tip. V-VAR technique consists of three steps. In the first step, the original dome point was marked. In patients with high tip projection and caudal rotation, resection was performed from the lateral crus of the original dome. In patients with high tip projection and cephalic rotation, resection was performed from the medial crus of the original dome. In patients with high nasal tip projection but adequate rotation, an equal amount of resections were performed from both the medial crus and lateral crus of the original dome. The patients were followed in average 18 months (between 12 and 24 months). RESULTS: The desired type of rotation and projection was achieved utilizing V-VAR technique in all patients. All patients had satisfactory esthetic results. CONCLUSIONS: In highly projected nasal tips, the height can be reduced using the proposed V-VAR technique. Rotation in the nasal tip region was maintained, increased or decreased in accordance with the esthetic aims.


Assuntos
Estética , Rinoplastia , Humanos , Rinoplastia/métodos , Feminino , Masculino , Adulto , Nariz/cirurgia , Adulto Jovem , Resultado do Tratamento , Pessoa de Meia-Idade , Cartilagens Nasais/cirurgia , Seguimentos , Reoperação/métodos
4.
Am J Otolaryngol ; 45(2): 104173, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38101140

RESUMO

BACKGROUND: Adjusting the nasal tip rotation and tip projection according to the patient's face and wishes is a very important stage in rhinoplasty with the use of a cog graft positioning the tip point in the appropriate place is possible by adjusting the nose length, tip projection and tip rotation together. OBJECTIVE: The aim of this study is to facilitate positioning the tip rotation and projection in the appropriate place according to the patient's needs in rhinoplasty with Cog graft. MATERIALS AND METHODS: Cog graft was applied in 32 patients who underwent rhinoplasty. Cog graft was prepared from costal cartilage in 10 patients and from septal cartilage in 22 patients. Cog graft was prepared by shaping the superior edge of the septal extension graft like a gear wheel. It is fixed to provide desired projection to the caudal septum. In the position where rotation and projection are evaluated adequately, the graft is fixed with sutures. They were followed in average 18 months (between 6 and 24 months). RESULTS: Twenty of the patients were primary cases and 12 were revision cases. The targeted rotation and projection results were achieved in all patients. All patients had satisfactory esthetic results. CONCLUSIONS: Cog graft is a useful graft with which we can adjust the nose length, projection and rotation appropriately and in a short time with a single graft.


Assuntos
Cartilagem Costal , Rinoplastia , Humanos , Rinoplastia/métodos , Septo Nasal/cirurgia , Nariz/cirurgia , Cartilagem/transplante , Cartilagem Costal/transplante , Estética , Estudos Retrospectivos , Cartilagens Nasais/cirurgia
5.
Eur Arch Otorhinolaryngol ; 281(4): 1827-1833, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38052758

RESUMO

PURPOSE: Nasal vascularization runs above the superficial musculoaponeurotic system (SMAS). Perichondrium covers the lower and upper lateral cartilages. In this study, nasal vascularization was compared between subperichondrial and supraperichondrial dissection in closed septorhinoplasty. METHODS: 95 patients and 41 volunteers were included in this study. Supraperichondrial dissection was performed in 48 patients and subperichondrial dissection was performed in 47 patients. To measure blood stream, laser doppler flowmetry (LDF) was used and measurements were done preoperatively, on the postoperative first week; 3rd month and first year. RESULTS: The nasal tip and dorsum measurements were similar between the preoperative and postoperative first year in both groups (p = 1.000). However, in the supraperichondrial dissection group, nasal tip measurements showed a significant increase between the preoperative and third postoperative months (p = 0.011). This increase was accompanied by an increase in the minimal blood stream (p = 0.014). CONCLUSION: Both subperichondrial and supraperichondrial dissection techniques are physiological and result in fewer complications with minimal permanent vascular damage. We believe incision plays a critical role but keeping the perichondrium intact is important for short-term angiogenesis, where long-term results showed no difference in vascularization.


Assuntos
Nariz , Rinoplastia , Humanos , Nariz/cirurgia , Rinoplastia/métodos , Dissecação/métodos , Período Pós-Operatório , Septo Nasal/cirurgia , Cartilagens Nasais/cirurgia
6.
Facial Plast Surg ; 40(5): 670-678, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-38325421

RESUMO

This experimental animal model study investigates the impact of different methods employed in preservation rhinoplasty (PR) on the strength of the nasal roof, focusing on three techniques: high strip, low strip, and intermediate strip. Using 15 lamb heads as surgical models, the study addresses key questions related to the strengths of each PR techniques, the influence of septal cartilage harvesting on septum strength, and the effectiveness of spreader grafts for stability. The research involves detailed dissection steps and measurements at various nasal points, evaluating the resistance at each stage. Results indicate that the low strip technique demonstrates the most significant reduction in strength. Furthermore, the combination of PR techniques with structural grafts, specifically spreader grafts, is assessed, revealing the classical rectangular spreader graft to be more effective in stabilizing the dorsum. Despite the limitation of using the lamb heads as models, this study offers valuable insights into the effects of PR on nasal septum strength and provides a foundation for further research on the biomechanics of preservation techniques.


Assuntos
Modelos Animais , Cartilagens Nasais , Septo Nasal , Rinoplastia , Animais , Rinoplastia/métodos , Septo Nasal/cirurgia , Ovinos , Cartilagens Nasais/cirurgia , Fenômenos Biomecânicos
7.
Facial Plast Surg ; 40(1): 80-85, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-37044127

RESUMO

We aimed to investigate the effect of estradiol on wound healing in an experimental nasal septal perforation (NSP) model created in rats. Twenty-two Sprague Dawley healthy male rats were separated into two groups. NSP, approximately 2 mm in diameter, was created in each animal. The 0.1 mL 0.12% estradiol (12 g/L) (study group) and 0.1 mL 0.9% saline (9 g/L) (control group) were applied topically once a day for 14 days. The rats were sacrificed on the 14th day and the cartilage nasal septum of each animal was excised for histopathological examination. The nasal mucosal epithelial regeneration and degeneration, acute inflammatory cell count, fibroblast number, vascularization, granulation tissue formation, giant cell number, eosinophil number, degeneration and regeneration of the nasal cartilage, and collagen density were examined. The macroscopic closure rate of the perforations and histopathological parameters were evaluated statistically. In this study, the epithelial regeneration, the fibroblast count, the granulation tissue formation, and the amount of collagen were significantly higher in the study group than in the saline group (p < 0.05). The acute inflammatory activity was lower in the estradiol group than in the saline group (p < 0.05). There was no statistically significant difference in the closure rate of perforation between the two groups (p = 0.163). No significant difference was found in other comparisons (p > 0.05). The locally administered estradiol may improve wound healing of the nasal septum in an experimental NSP animal model. LEVEL OF EVIDENCE: Not applicable.


Assuntos
Perfuração do Septo Nasal , Masculino , Ratos , Animais , Perfuração do Septo Nasal/tratamento farmacológico , Ratos Sprague-Dawley , Cicatrização , Modelos Animais , Colágeno , Cartilagens Nasais
8.
Aesthetic Plast Surg ; 48(15): 2951-2964, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38528127

RESUMO

INTRODUCTION: Since 3D printing can be used to design implants according to the specific conditions of patients, it has become an emerging technology in tissue engineering and regenerative medicine. How to improve the mechanical, elastic and adhesion properties of 3D-printed photocrosslinked hydrogels is the focus of cartilage tissue repair and reconstruction research. MATERIALS AND METHODS: We established a strategy for toughening hydrogels by mixing GelMA-DOPA (GD), which is prepared by coupling dopamine (DA) with GelMA, with HAMA, bacterial cellulose (BC) to produce composite hydrogels (HB-GD). HB-GD hydrogel scaffolds were characterized in vitro by scanning electron microscopy (SEM), Young's modulus, swelling property and rheological property tests. And biocompatibility and chondrogenic ability were tested by live/dead staining, DNA quantitative analysis and immunofluorescence staining. Combined with 3D bioprinting technology, mouse chondrocytes (ADTC5) were added to form a biological chain to construct an in vitro model, and the feasibility of the model for nasal cartilage regeneration was verified by cytology evaluation. RESULTS: With the increase of GD concentration, the toughness of the composite hydrogel increased (47.0 ± 2.7 kPa (HB-5GD)-158 ± 3.2 kPa (HB-20GD)), and it had excellent swelling properties, rheological properties and printing properties. The HB-GD composite hydrogel promoted the proliferation and differentiation of ATDC5. Cells in 3D printed scaffolds had higher survival rates (> 95%) and better protein expression than the encapsulated cultures. CONCLUSION: The HB-10GD hydrogel can be made into a porous scaffold with precise shape, good internal pore structure, high mechanical strength and good swelling rate through extrusion 3D printing. NO LEVEL ASSIGNED: This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.


Assuntos
Bioimpressão , Dopamina , Hidrogéis , Cartilagens Nasais , Impressão Tridimensional , Engenharia Tecidual , Alicerces Teciduais , Bioimpressão/métodos , Camundongos , Animais , Engenharia Tecidual/métodos , Cartilagens Nasais/cirurgia , Teste de Materiais , Materiais Biocompatíveis , Condrócitos
9.
Aesthetic Plast Surg ; 48(16): 3098-3108, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38483551

RESUMO

BACKGROUND: This study introduces and assesses the outcomes of a novel rhinoplasty technique, TRICK-TIP (Transcolumellar and Inter Cartilaginous Keystoning with Tip preservation), employing a combined open and closed approach with tip anatomy conservation and structured tip support. METHODS: The procedure involves a low stairstep columellar sectioning, followed by transmembranous and intercartilaginous incisions without skin dissection in the columella or tip. Elevating the entire mobile nose as a three-layered flap provides extensive access to the entire nasal pyramid and septum. Tip modifications, including retrograde cephalic cartilage resection and supratip skin thinning, are performed based on individual cases. A key columellar strut is frequently used, initially sutured in the interdomal space and then turned down for height adjustment and final fixation. Interdomal sutures, supratip sutures, and alar resection are implemented as needed. RESULTS: One hundred twenty patients participated, with high satisfaction and a low frequency of adverse effects reported using four FACE-Q™ questionnaires. One hundred and two independent raters evaluated pre and postoperative photographs, scoring "overall nose result" as 3.6 out of 5, with minimal or absent nostril deformities (1.84), soft triangle deformities (1.73), and columellar external scar deformity/visibility (1.35) where 1 is the absence of the deformity and 5 is disfigurement. Complications were absent, and revisions were infrequent. CONCLUSIONS: The combined benefits of the wide-open approach, shortened surgery duration, and nasal tip preservation contribute to outcome optimization. TRICK-TIP rhinoplasty is characterized by simplicity, enabling targeted modifications, preventing soft triangle and rim complications, and facilitating essential tip support while maintaining favorable results. Level of Evidence V This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .


Assuntos
Estética , Satisfação do Paciente , Rinoplastia , Humanos , Rinoplastia/métodos , Feminino , Adulto , Masculino , Adulto Jovem , Pessoa de Meia-Idade , Resultado do Tratamento , Técnicas de Sutura , Cartilagens Nasais/cirurgia , Septo Nasal/cirurgia , Estudos de Coortes , Nariz/cirurgia , Retalhos Cirúrgicos/transplante , Adolescente , Estudos Retrospectivos
10.
Cleft Palate Craniofac J ; 61(4): 592-598, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36604781

RESUMO

INTRODUCTION: In secondary cleft lip and nasal deformity (CLND) correction, structural grafts are commonly used to control the nasal tip and restore the symmetry of the ala. However, the septal cartilage in Asians often weak and small. Biocompatible absorbable materials are alternatives to autologous grafts. This study assessed the surgical outcomes and complications of poly lactic-co-glycolic acid (PLGA) plate grafts in secondary CLND correction. METHODS: This study was retrospectively analyzed for patients who underwent secondary rhinoplasty for unilateral CLND correction between March 2015 and November 2020. Using open rhinoplasty, the PLGA plate was grafted as a columellar strut. Clinical photographs taken at the initial (T0) and follow-up visits (T1: short-term, T2: long-term) were analyzed and anthropometric parameters, such as nostril height and width, dome height, and tip height, were measured. RESULTS: Twenty-four patients were included in this study. The mean T1 and T2 periods were 1.0 ± 0.4 and 15.5 ± 3.1 months, respectively. The nostril height ratio increased from 0.78 ± 0.12 at T0 to 0.88 ± 0.08 at T1 and 0.86 ± 0.09 at T2 (p < 0.001; Relapse ratio -2.6 ± 6.7%). The tip height ratio increased from 0.60 ± 0.07 (T0) to 0.66 ± 0.05 (T2) (Relapse ratio -3.7 ± 3.0%). CONCLUSIONS: The PLGA plate graft provided stable nasal tip projection and alar symmetry without major complications. It can be a good option for patients lacking available septal and concha cartilages or apprehensive of additional scarring.


Assuntos
Fenda Labial , Implantes Dentários , Glicolatos , Rinoplastia , Humanos , Fenda Labial/cirurgia , Estudos Retrospectivos , Glicóis , Cartilagens Nasais/transplante , Resultado do Tratamento , Nariz/cirurgia , Septo Nasal/cirurgia , Recidiva
11.
Dev Dyn ; 252(7): 1009-1025, 2023 07.
Artigo em Inglês | MEDLINE | ID: mdl-36880689

RESUMO

BACKGROUND: We previously determined a nonlinear relationship between connexin 43 (Cx43) function and craniofacial phenotypic variation in the mutant mouse model G60S/+, and that this variation was driven by nasal bone deviation. While nonlinearities in the genotype-phenotype map appear to be common, few studies have looked at the developmental processes that underlie this nonlinearity. Here, we investigated the potential tissue-level developmental determinants of the variation in nasal bone phenotype in G60S/+ mice through postnatal development. RESULTS: The deviated nasal bone phenotype arises by postnatal day 21 and becomes more severe by 3 months in G60S/+ mice. Measures of nasal bone remodeling including the number of osteoclasts, mineralizing surface, mineral apposition rate, and bone formation rate are significantly greater in G60S/+ mice compared to wild-type mice at 2 months, but these differences do not correspond with nasal bone deviation. The degree of nasal bone deviation does significantly and negatively correlate with the ratio between nasal bone and cartilaginous nasal septum length. CONCLUSIONS: Our findings indicate that the mean phenotypic changes observed between G60S/+ and wild-type mice are due to reduced bone growth, but the increased phenotypic variation found within mutant mice is due to discordant growth between nasal cartilage and bone.


Assuntos
Cartilagens Nasais , Crânio , Camundongos , Animais , Septo Nasal , Fenótipo
12.
Aesthet Surg J ; 44(10): 1023-1031, 2024 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-38648541

RESUMO

BACKGROUND: Various techniques are employed for septal perforation repair but success rates still vary. Numerous mucosal flaps are used for septal perforation closure; however, a scaffold is essential for proper positioning and enhanced mucosal growth. OBJECTIVES: The aim of this study was to assess the effectiveness of utilizing a septal bone/cartilage composite graft (BC unit) to close septal perforations in rhinoplasty patients and evaluate patient satisfaction. METHODS: This case series included patients with septal perforation undergoing rhinoplasty and perforation repair between 2019 and 2023. BC units were used to cover the cartilaginous perforations in all patients. Patients were followed for a minimum of 1 year, assessing surgical results and postoperative satisfaction with the 10-item Standardized Cosmesis and Health Nasal Outcomes Survey (SCHNOS). RESULTS: In this study, 31 patients (22 female, 9 male), with a mean [standard deviation] age of 34.80 [10.31] years (range, 19-59 years), were enrolled. Nine had previousl undergone septoplasty, while four had undergone septorhinoplasty. The preoperative perforation size ranged from 6 to 27 mm (mean, 14.1 mm). At final follow-up, 26 cases (83.8%) achieved complete closure, while 3 had incomplete closures, and 2 experienced reperforation. Analysis revealed a notable mean decrease of 31.93 [26.47] in obstruction domain scores (SCHNOS-O) and a mean decrease of 44.19 [25.37] in cosmesis domain scores (SCHNOS-C). CONCLUSIONS: Septal perforation repair and rhinoplasty can be safely and effectively performed concurrently with BC units for suitable candidates.


Assuntos
Perfuração do Septo Nasal , Satisfação do Paciente , Rinoplastia , Humanos , Rinoplastia/métodos , Rinoplastia/efeitos adversos , Feminino , Masculino , Adulto , Pessoa de Meia-Idade , Adulto Jovem , Perfuração do Septo Nasal/cirurgia , Resultado do Tratamento , Septo Nasal/cirurgia , Cartilagens Nasais/cirurgia , Osso Nasal/cirurgia , Osso Nasal/lesões , Seguimentos , Transplante Ósseo/métodos , Transplante Ósseo/efeitos adversos , Estudos Retrospectivos
13.
Turk J Med Sci ; 54(2): 431-440, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39050393

RESUMO

Background/aim: Herein, we describe a new technique to obtain both the appropriate degree of rotation angle and the ideal nasal bridge length. The aim of this study is to investigate the long-term results of this new technique with regard to these two variables. Materials and methods: A total of 76 (27 males, 49 females) patients were operated in accordance with the presented technique. Internal caudal mucosal excision and lower lateral cartilage (LLC) suspension were applied to all the patients included in this prospective study. Preoperative, immediate postoperative, and postoperative 1st-year photographs were taken. NOSE scores were obtained in the postoperative 1st year. Results: The mean nasolabial angle values of the patients preoperatively, at the end of the surgery (immediate postoperative), and at the end of the first year were 94.13° ± 5.1, 113.1° ± 5.3, and 109.6° ± 5.2, respectively. The patients had an average gain of 19° at the nasolabial angle at the end of the surgery and experienced a 3.5° (3.1%) loss at the end of the first year. For the nasal bridge length (n-prn) values; the preoperative, immediate postoperative, and first year mean values were 5.1 ± 0.55 cm, 3.98 ± 0.41 cm, and 4.29 ± 0.39 cm, respectively. The noses of the patients were shortened by 1.11 cm on average at the end of the surgery. Conclusion: Internal caudal mucosal resection with a suspension of the LLC to the caudal edge of the upper lateral cartilages (ULC) offers a reliable method to control the nasal tip rotation and shorten the long noses. This technique's effect is more obvious in long noses compared to the short ones.


Assuntos
Rinoplastia , Humanos , Masculino , Feminino , Adulto , Estudos Prospectivos , Rinoplastia/métodos , Pessoa de Meia-Idade , Mucosa Nasal/cirurgia , Adulto Jovem , Cartilagens Nasais/cirurgia , Rotação , Resultado do Tratamento , Nariz/cirurgia , Nariz/anatomia & histologia
14.
Biochem Biophys Res Commun ; 678: 173-178, 2023 10 20.
Artigo em Inglês | MEDLINE | ID: mdl-37640003

RESUMO

Bone morphogenetic proteins (BMPs) are required for craniofacial bone development. However, it remains elusive how BMP signaling regulates craniofacial cartilage development. To address this question, we utilized a genetic system to enhance BMP signaling via one of BMP type I receptors ALK2 in a chondrocyte-specific manner (hereafter Ca-Alk2:Col2-Cre) in mice. Ca-Alk2:Col2-Cre mice died shortly after birth due to severe craniofacial abnormalities including cleft palate, defective tongue, and shorter mandible formation. Histological analysis revealed that these phenotypes were attributed to the extensive chondrogenesis. Compared with controls, enhanced SOX9 and RUNX2 production were observed in nasal cartilage of Ca-Alk2:Col2-Cre mice. To reveal the mechanisms responsible for enlarged nasal cartilage, we examined Smad-dependent and Smad-independent BMP signaling pathways. While the Smad-independent BMP signaling pathway including p38, ERK, and JNK remained silent, the Smad1/5/9 was highly phosphorylated in Ca-Alk2:Col2-Cre mice. Interestingly, Ca-Alk2:Col2-Cre mice showed enhanced S6 kinase phosphorylation, a readout of mammalian target of rapamycin complex 1 (mTORC1). These findings may suggest that enhanced Smad-dependent BMP signaling positively regulates the mTOR pathway and stimulates chondrocytes toward hypertrophic differentiation, thereby leading to enlarged nasal cartilage formation in mice.


Assuntos
Fissura Palatina , Cartilagens Nasais , Animais , Camundongos , Condrogênese , Nariz , Transdução de Sinais , Mamíferos
15.
Biosci Biotechnol Biochem ; 88(1): 107-110, 2023 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-37881018

RESUMO

Hair loss is a commonly encountered problem. In this study, hair growth was enhanced by daily oral ingestion of salmon nasal cartilage proteoglycan (PG) in mice. Proteoglycan stimulated vesicular endothelial growth factor production in human follicle dermal papilla cells through insulin growth factor-1 receptor signaling, suggesting the possibility of hair loss improvement by PG ingestion.


Assuntos
Cartilagens Nasais , Proteoglicanas , Humanos , Animais , Camundongos , Proteoglicanas/farmacologia , Proteoglicanas/metabolismo , Salmão , Cabelo , Alopecia , Folículo Piloso , Células Cultivadas
16.
Am J Otolaryngol ; 44(4): 103817, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36905778

RESUMO

OBJECTIVE: The aim of this study is to demonstrate a novel surgical technique in the treatment of lower lateral crural protrusion and external nasal valve pinching. METHOD: The lower lateral crural resection technique was used in 24 patients who underwent open technique septorhinoplasty between 2019 and 2022. Fourteen of the patients were female and 10 were male. In this technique, excess part of crura's tail was excised from the lower lateral crura and placed in the same pocket. This area was supported with a diced cartilage and a postoperative nasal retainer was applied. We have corrected the aesthetic problem that occurs when the lower lateral cartilage is convex, and external nasal valve pinching that occurs when the lower lateral crural protrusion is concave. RESULT: The mean age of the patients was 23. The mean follow-up time of the patients was between 6 and 18 months. No complications were observed due to this technique. Satisfactory results were obtained in the postoperative period after surgery. CONCLUSION: A new surgical approach has been proposed for patients with lower lateral crural protrusion and external nasal valve pinching using the lateral crural resection technique.


Assuntos
Nariz , Rinoplastia , Humanos , Masculino , Feminino , Nariz/cirurgia , Cartilagem/cirurgia , Rinoplastia/métodos , Estética , Retalhos Cirúrgicos , Cartilagens Nasais/cirurgia
17.
Ann Plast Surg ; 90(1 Suppl 1): S2-S9, 2023 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-36752416

RESUMO

BACKGROUND: LeFort I osteotomy changes the morphology of the nose. The cinch suture has been proven to prevent the increase in nasal base and alar width. Different types of cinch sutures have been proposed. However, their effectiveness is unclear. AIM AND OBJECTIVES: The aim of this study was to compare the surgical outcomes between conventional and modified cinch techniques through a systematic review and meta-analysis of randomized control trials (RCTs). MATERIAL AND METHODS: We performed systematic search from Embase, PubMed, and the Cochrane Library according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement up to March 2021. The surgical techniques of different cinch sutures were reviewed, and the outcomes of nasal alar width and alar base width were compared between modified and conventional methods. RESULTS: A total of 4 eligible RCTs were included in this meta-analysis. Pooled data showed no significant difference in alar base width change between modified and conventional methods (mean difference, -0.37; 95% confidence interval, -1.32 to 0.57; P = 0.44). Pooled data of 3 studies also showed no significant difference in nasal alar width change (mean difference, -0.11; 95% confidence interval, -1.18 to 0.95; P = 0.83). CONCLUSION: Based on the current data pooled from the available RCTs, no significant difference was found between the conventional cinch technique and the modified technique.


Assuntos
Maxila , Cartilagens Nasais , Humanos , Cartilagens Nasais/cirurgia , Maxila/cirurgia , Osteotomia de Le Fort/métodos , Nariz/cirurgia , Técnicas de Sutura
18.
J Craniofac Surg ; 34(6): e558-e561, 2023 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-37246295

RESUMO

Nasal tip surgery is a crucial aspect of rhinoplasty in which suture techniques play a significant role. Early suturing techniques focused primarily on repositioning alar cartilage remnants after they had undergone significant resection. The size, contour, and orientation of the medial and lateral crura are primary factors in creating a tip shape. In this review, we retrospectively evaluated obliquely oriented dome sutures with triangular dome resection that was performed in 540 rhinoplasty cases at Yunus Emre Hospital between 2015 and 2020. Dome-defining sutures were placed, and a triangular cartilage resection was performed. Subsequently, oblique sutures were placed to achieve the desired lateral cartilage position. Objective assessment of postoperative results (Objective Rhinoplasty Outcome Score), patient satisfaction assessments, and nasal examinations were conducted. The objective assessments of the esthetic results showed a significant improvement, with a mean score of 3.6, which represents a good to excellent outcome. Most patients were subjectively satisfied with the surgical outcomes of rhinoplasty. No serious complications, such as infection, recurrence of deviation, nasal obstruction, or esthetic problems such as dorsal irregularities, were observed after surgery. Overall, suturing techniques play an important role in determining the nasal tip shape. Our technique is beneficial for maintaining a favorable lateral crural position, leading to improved patient satisfaction.


Assuntos
Estética Dentária , Rinoplastia , Humanos , Estudos Retrospectivos , Nariz/cirurgia , Rinoplastia/métodos , Cartilagens Nasais/cirurgia , Técnicas de Sutura
19.
J Craniofac Surg ; 34(7): 1971-1977, 2023 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-37322585

RESUMO

The purposes of this study were to analyze the effect of trans-sutural distraction osteogenesis (TSDO) on nasal bone, nasal septum, and nasal airway in the treatment of midfacial hypoplasia. A total of 29 growing patients with midfacial hypoplasia who underwent TSDO by a single surgeon were enrolled. The 3-dimensional measurement of nasal bone and nasal septum changes was performed using computed tomography (CT) images obtained preoperatively (T0) and postoperatively (T1). One patient was selected to establish 3-dimensional finite element models to simulate the characteristics of nasal airflow field before and after traction. After traction, the nasal bone moved forward significantly ( P <0.01). The septal deviation angle was lower than that before traction (14.43±4.70 versus 16.86 ±4.59 degrees) ( P <0.01). The length of the anterior and posterior margin of the vomer increased by 21.4% ( P <0.01) and 27.6% ( P <0.01), respectively, after TSDO. The length of the posterior margin of the perpendicular plate of ethmoid increased ( P <0.05). The length of the posterior inferior and the posterior superior margin of the nasal septum cartilage increased ( P <0.01) after traction. The cross-sectional area of nasal airway on the deviated side of nasal septum increased by 23.0% after traction ( P <0.05). The analysis of nasal airflow field showed that the pressure and velocity of nasal airflow and the nasal resistance decreased. In conclusion, TSDO can promote the growth of the midface, especially nasal septum, and increase the nasal space. Furthermore, TSDO is conductive to improve nasal septum deviation and decrease nasal airway resistance.


Assuntos
Osso Nasal , Osteogênese por Distração , Humanos , Osteogênese por Distração/métodos , Septo Nasal/diagnóstico por imagem , Septo Nasal/cirurgia , Septo Nasal/anormalidades , Face , Cartilagens Nasais
20.
J Craniofac Surg ; 34(6): 1686-1689, 2023 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-37322590

RESUMO

INTRODUCTION: This study aimed to evaluate the effectiveness of the triple strut graft technique for nasal tip projection in Asian patients with weak and small lower lateral cartilages and septum. The technique uses a combination of septal angle strut and, columellar strut grafts and lateral crural repositioning to provide support for the nasal tip. METHODS: The study included 30 Asian patients who underwent primary rhinoplasty using this technique between January 2019 and December 2021. The surgical procedure involved making an open rhinoplasty incision and performing a scroll area release. A small triangular-shaped septal angle strut graft was then placed, and the lower lateral cartilages were suspended anteriorly and placed on the anterior end of the septal angle after a columellar strut graft was performed between both medial crura. The lateral crura of the lower lateral cartilages were transposed medially on top of the upper lateral cartilages and maintained in place by spanning sutures at the cephalic margins of both lateral crura. RESULTS: The triple strut graft technique was shown to be effective in providing stable tip projection in Asian noses with weak and small lower lateral cartilages and septum. Statistically significant differences were found between the preoperative and postoperative values of the nasal tip projection ratio and Rhinoplasty Outcome Evaluation ( P < 0.05). CONCLUSIONS: The triple strut graft tip projection technique can be an effective surgical option for Asian patients who have weak and small medial crura combined with a small septum, providing stability for the nasal tip projection.


Assuntos
Implantes Dentários , Rinoplastia , Humanos , Nariz/cirurgia , Septo Nasal/cirurgia , Cartilagem/transplante , Rinoplastia/métodos , Avaliação de Resultados em Cuidados de Saúde , Cartilagens Nasais/transplante
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