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1.
ISME J ; 11(4): 1029-1042, 2017 04.
Artigo em Inglês | MEDLINE | ID: mdl-27959343

RESUMO

The SaPIs are a cohesive subfamily of extremely common phage-inducible chromosomal islands (PICIs) that reside quiescently at specific att sites in the staphylococcal chromosome and are induced by helper phages to excise and replicate. They are usually packaged in small capsids composed of phage virion proteins, giving rise to very high transfer frequencies, which they enhance by interfering with helper phage reproduction. As the SaPIs represent a highly successful biological strategy, with many natural Staphylococcus aureus strains containing two or more, we assumed that similar elements would be widespread in the Gram-positive cocci. On the basis of resemblance to the paradigmatic SaPI genome, we have readily identified large cohesive families of similar elements in the lactococci and pneumococci/streptococci plus a few such elements in Enterococcus faecalis. Based on extensive ortholog analyses, we found that the PICI elements in the four different genera all represent distinct but parallel lineages, suggesting that they represent convergent evolution towards a highly successful lifestyle. We have characterized in depth the enterococcal element, EfCIV583, and have shown that it very closely resembles the SaPIs in functionality as well as in genome organization, setting the stage for expansion of the study of elements of this type. In summary, our findings greatly broaden the PICI family to include elements from at least three genera of cocci.


Assuntos
Bacteriófagos/fisiologia , Regulação Bacteriana da Expressão Gênica/fisiologia , Ilhas Genômicas , Cocos Gram-Positivos/virologia , Sítios de Ligação Microbiológicos
2.
Gene ; 226(1): 83-93, 1999 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-9889328

RESUMO

The nucleotide sequence of a DNA fragment previously shown to contain the attachment site (attP) of Oenococcus oeni phage fOg44 (. Arch. Virol. 143, 523-536) has been determined. Sequence analysis indicated that this 6226bp EcoRI fragment harbours an integrase gene, in the vicinity of a direct repeat rich region defining attP, as well as genes encoding a muramidase-related lysin (Lys) and a holin polypeptide (Hol). Transcriptional studies suggested that lys and hol are mainly co-expressed, late in the lytic cycle, from a promotor located upstream of lys. Between the lytic cassette and the phage integration elements three additional open reading frames were found: orf217 and orf252 of unknown function and orf72, the putative product of which bears 32% identity with acidic excisionases from other Gram positive phages. We have established that the first two orfs, as well as the predicted promotor of orf72, are included in a 2143-bp DNA segment missing from the genome of the deletion mutant fOg44Delta2. Although lysogens of fOg44 and fOg44Delta2 exhibited similar properties, each phage produced two distinguishable types of lysogenic strains, differing in inducibility and immunity to other oenophages.


Assuntos
Bacteriófagos/genética , Genes Virais , Lactobacillaceae/virologia , Lisogenia/genética , Sequência de Aminoácidos , Bacteriófagos/imunologia , Sequência de Bases , Desoxirribonuclease EcoRI , Deleção de Genes , Cocos Gram-Positivos/virologia , Integrases/genética , Integrases/metabolismo , Leuconostoc/virologia , Lisogenia/imunologia , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Dados de Sequência Molecular , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Análise de Sequência de DNA , Proteínas Virais/genética , Proteínas Virais/metabolismo
3.
FEMS Microbiol Lett ; 171(2): 231-8, 1999 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-10077848

RESUMO

Malolactic fermentation by Oenococcus oeni is a crucial step in wine-making. Oe. oeni phages are thought to be responsible for fermentation failures, yet they have received little attention. After a molecular analysis concerning the phage phi 10MC integration system, this paper focuses on the lytic system. The attP (phage attachment site)-flanking region has been cloned and sequenced. The 1296-bp lysin gene (Lys) was identified in this region. The deduced amino acid sequence showed classical structural features of phage lysins, and this gene product expressed in Escherichia coli had a lytic activity against Oe. oeni. Downstream of Lys, a second ORF was present (P163). According to its amino acid sequence and the location of its gene, the product could be the phi 10MC holin. This study shows that the genomic organization of phage phi 10MC attP-flanking regions is very similar to that of other lactic acid bacteriophages.


Assuntos
Bacteriófagos/genética , Genes Virais/genética , Vinho/virologia , Clonagem Molecular , DNA Viral/análise , Endopeptidases/genética , Escherichia coli , Fermentação , Regulação Viral da Expressão Gênica , Genoma Viral , Cocos Gram-Positivos/metabolismo , Cocos Gram-Positivos/virologia , Leuconostoc/metabolismo , Leuconostoc/virologia , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Plasmídeos , Homologia de Sequência de Aminoácidos , Proteínas Virais/genética , Vinho/microbiologia
4.
Arch Virol ; 143(3): 523-36, 1998.
Artigo em Inglês | MEDLINE | ID: mdl-9580499

RESUMO

The genome structure of six bacteriophages of Oenococcus oeni was compared. Two distinct groups with no apparent restriction site conservation were defined. In members of the alpha group (fOgML34, fOg4029, fOg30 and fOg218) a 7.5 kb region containing the origin of DNA packaging (cos) was highly conserved. Stretches of DNA heterogeneity could also be assigned to particular regions and were mostly evident in the right area of the genomes. fOg44 and fOgPSU1 (beta group) were indistinguishable in the left half of their genomes, including cos, but were markedly dissimilar in other regions. Strong labelling signals detected in cross-hybridizations involving members of different groups were confined to fragments centrally located in their physical maps. The attachment site (attP) of fOg44 was assigned to this conserved region. It is suggested that recombination events at this location may have been important in generating the observed diversity of oenophage genomes.


Assuntos
Bacteriófagos/genética , Variação Genética , Genoma Viral , Clonagem Molecular , DNA Viral , Cocos Gram-Positivos/virologia , Leuconostoc/virologia , Hibridização de Ácido Nucleico , Mapeamento por Restrição
5.
Curr Microbiol ; 36(6): 365-9, 1998 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-9608749

RESUMO

A large number of strains of Oenococcus oeni (formerly Leuconostoc oenos) that had been isolated from wines were checked for lysogeny with mitomycin C as inducer. As a result of this test, 45% of the strains proved to be lysogenic, suggesting that lysogeny is widespread among bacteria isolated from wines during malolactic fermentation. The sensitivity of bacteria to phages was very different, depending on the strain. All the lysogenic strains were resistant to infection by the temperate phage they released. Some phages infected none of the strains. Phages of Oenoc. oeni had a classical morphology, an isometric head, and a long striated tail. With the broadest host strain as an indicator, phages were detected in wines after malolactic fermentation.


Assuntos
Bacteriófagos/isolamento & purificação , Cocos Gram-Positivos/virologia , Lisogenia , Bacteriófagos/fisiologia , Bacteriófagos/ultraestrutura , Meios de Cultura , Leuconostoc/virologia , Mitomicina/farmacologia , Vinho
6.
Virology ; 325(1): 82-95, 2004 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-15231388

RESUMO

The central genomic regions of Oenococcus oeni phages fOg30 and fOgPSU1 have been compared with the equivalent regions of oenophages fOg44 and phi 10MC. In all cases, an almost identical endolysin gene was followed by one of two orfs, encoding putative holins (orf117 and orf163). The fOg44 endolysin was established as a secretory protein when expressed in Lactococcus lactis. Orf117 (from fOg44) promoted lysis of Escherichia coli cultures upon induction of a defective lambda Sam7 prophage, but Orf163 (from fOg30) failed to elicit a lysis response in this system. fOg44 and fOgPSU1 were shown to integrate at the 3' end of a tRNA(Glu) and a tRNA(Lys), respectively. Searching the available sequence of the O. oeni MCW genome for attP-like elements, two other tRNA targets could be proposed for prophage establishment. Between the lysis and integration elements, a diverse cluster of genes (absent in phi 10MC) was observed. One common gene in this "lysogenic conversion cluster" was experimentally confirmed as a transcriptional repressor, affecting the expression of a putative permease gene.


Assuntos
Bacteriófagos/genética , Genoma Viral , Cocos Gram-Positivos/virologia , RNA de Transferência/genética , Integração Viral , Sequência de Aminoácidos , Sequência de Bases , Endopeptidases/metabolismo , Leuconostoc/virologia , Lisogenia , Dados de Sequência Molecular
7.
J Bacteriol ; 182(20): 5823-31, 2000 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11004183

RESUMO

The function of the N-terminal region of the Oenococcus oeni phage fOg44 lysin (Lys44) as an export signal was investigated. We observed that when induced in Escherichia coli, Lys44 was cleaved between residues 27 and 28 in a SecA-dependent manner. Lys44 processing could be blocked by a specific signal peptidase inhibitor and was severely reduced by modification of the cleavage site. The lethal effect of Lys44 expression observed in E. coli was ascribed to the presence of its N-terminal 27-residue sequence, as its deletion resulted in the production of a nontoxic, albeit active, product. We have further established that lytic activity in oenococcal cells was dependent on Lys44 processing. An active protein with the molecular mass expected for the cleaved enzyme was detected in extracts from O. oeni-infected cells. The temporal pattern of its appearance suggests that synthesis and export of Lys44 in the infected host progress along with phage maturation. Overall, these results provide, for the first time, experimental evidence for the presence of a signal peptide in a bacteriophage lysin. Database searches and alignment of protein sequences support the prediction that other known O. oeni and Lactococcus lactis phages also encode secretory lysins. The evolutionary significance of a putative phage lysis mechanism relying on secretory lytic enzymes is tentatively discussed, on the basis of host cell wall structure and autolytic capacity.


Assuntos
Bacteriófagos/metabolismo , Escherichia coli/metabolismo , Cocos Gram-Positivos/virologia , Proteínas Virais/genética , Proteínas Virais/metabolismo , Sequência de Aminoácidos , Sequência de Bases , Parede Celular/enzimologia , Clonagem Molecular , Escherichia coli/virologia , Hidrolases/metabolismo , Cinética , Leuconostoc/virologia , Dados de Sequência Molecular , Sinais Direcionadores de Proteínas , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Proteínas Virais/química
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