[Identification of Corydalis yanhusuo,C. turtschaninovii,C. decumbens by allele-specific PCR].

To establish a DNA molecular markers method for identification of Corydalis yanhusuo,C. turtschaninovii and C. decumbens,the mat K,trn G and psb A-trn H sequences of 56 samples from 14 species of C. yanhusuo,C. turtschaninovii,C. decumbens and their related species were obtained by sequencing. The SNP loci were obtained by Bio Edit 7. 2. 2 software. The primers for AS-PCR identification were designed based on the mutation sites,and the conditions of PCR were optimized to identify C. yanhusuo,C. turtschaninovii,and C. decumbens according to the specific bands. The results showed that the amount of template( 0. 6-1 200 ng)and annealing temperature( 42-60 ℃) had little influence on the amplification results,and the number of cycles had much influence on the amplification results. When the number of cycles was 20,the specific bands of 297 bp( mat K),353 bp( trn G) and 544 bp( mat K) were amplified from C. yanhusuo,C. turtschaninovii and C. decumbens,respectively. The method established in this study had a minimum detection limit of 6 ng for C. yanhusuo,60 ng for C. decumbens and less than 0. 6 ng for C. turtschaninovii. Thus,the allelespecific PCR method established in the research can specifically identify C. yanhusuo,C. turtschaninovii,and C. decumbens.

[Identification and analysis of Corydalis boweri, Meconopsis horridula and their close related species of the same genus by using ITS2 DNA barcode].

The study is aimed to ensure the quality and safety of medicinal plants by using ITS2 DNA barcode technology to identify Corydalis boweri, Meconopsis horridula and their close related species. The DNA of 13 herb samples including C. boweri and M. horridula from Lhasa of Tibet was extracted, ITS PCR were amplified and sequenced. Both assembled and web downloaded 71 ITS2 sequences were removed of 5. 8S and 28S. Multiple sequence alignment was completed and the intraspecific and interspecific genetic distances were calculated by MEGA 5.0, while the neighbor-joining phylogenetic trees were constructed. We also predicted the ITS2 secondary structure of C. boweri, M. horridula and their close related species. The results showed that ITS2 as DNA barcode was able to identify C. boweri, M. horridula as well as well as their close related species effectively. The established based on ITS2 barcode method provides the regular and safe detection technology for identification of C. boweri, M. horridula and their close related species, adulterants and counterfeits, in order to ensure their quality control, safe medication, reasonable development and utilization.

[Phytochemical and pharmacological advance on Tibetan medicinal plants of Corydalis].

It was estimated that about 428 species of genus Corydalis are distributed all worldwide, with about 298, especially 10 groups and 219 species being uniquely spread in China. The genus Corydalis have been widely employed as folk medicines in China, especially as traditional Tibetan medicines, for treatment of fever, hepatitis, edema, gastritis, cholecystitis, hypertension and other diseases. The phytochemical studies revealed that isoquinoline alkaloids are its major bioactive ingredients. The extensive biological researches suggested its pharmacological activities and clinic applications against cardiovascular diseases and central nervous system, antibacterial activities, analgesic effects, anti-inflammatory, anti-oxidation and anti-injury for hepatocyte, and so on. As an effort in promoting the research of pharmacodynamic ingredients, this article presents an overview focusing on the distribution, phytochemical and pharmacological results of Corydalis species that have been applied in traditional Tibetan medicinal, hopefully to provide a reference for the new Tibetan medicine development from Corydalis plant resource.

[Quality control of Corydalis Yanhusuo W. T. Wang by second derivative FTIR spectroscopy combined with statistics].

A new method using single reflection Fourier transform infrared spectroscopy was proposed for direct and fast determination of Corydalis yanhusuo W. T. Wang of traditional Chinese herbal medicines and its confusable varieties. Data were collected directly for Fourier transform infrared spectra with OMNI sampler. Then through converting FTIR spectra of the samples into second derivative spectra by derivative spectra software, it is possible to identify Corydalis yanhusuo W. T. Wang from the confusable varieties with statistics. The result shows that the second derivative FTIR of Corydalis yanhusuo W. T. Wang and its confusable varieties are different in the 2 050-650 cm(-1) range. The probability is less than 0.01 and the result is significant. Corydalis yanhnsuo W. T. Wang and its confusable varieties can be identified by identifying the inner layer parts of the cuticles of samples by second derivative FTIR spectroscopy with statistics directly, rapidly and accurately.

[Infrared spectral identification of rhizoma Corydalis and rhizoma Corydalis repentis].

OBJECTIVE: To study the IR spectra of Rhizoma Corydalis and Rhizoma Corydalis Repentis. METHOD: The petroleum ether, ethyl ether and water extracts obtained by the same isolating procedure from Rhizoma Corydalis and Rhizoma Corydalis Repentis were determined by IR spectrophotometry. RESULTS: The IR spectral evidences of the two samples showed distinct characteristics and good repeatability. CONCLUSION: The IR spectral evidences can be used to differentiate Rhizoma Corydalis and Rhizoma Corydalis Repentis.