Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 33
Filtrar
Mais filtros

Base de dados
Tipo de documento
Intervalo de ano de publicação
1.
Rapid Commun Mass Spectrom ; 38(22): e9902, 2024 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-39226915

RESUMO

RATIONALE: Anabolic steroids, also known as anabolic-androgenic steroids (AAS), encompass steroidal androgens such as testosterone, as well as synthetic counterparts with similar structures and effects. The misuse of AAS has increased over the years, leading to ethical and welfare concerns in sports. The World Anti-Doping Agency (WADA) and the International Federation for Equestrian Sports (FEI) have banned AAS in relevant sports. Methandienone is one of the most identified anabolic androgenic steroids in sports drug testing, Therefore, reliable detection methods are crucial for effective doping control and maintaining the integrity of the sports. METHODS: This study explores the use of homogenized camel liver for detecting methandienone metabolites in camels. The biotransformation pathways of methandienone in homogenized camel liver tissues are analyzed using Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) to identify and characterize the phase I and phase II metabolites. Chromatographic separation was achieved using a Thermo-Hypersil C18 column. RESULTS: The study has identified 11 methandienone metabolites (M1-M11), this includes 10 phase I and one phase II metabolite. A glucuronic acid conjugate of methandienone was observed in this study, but no sulfonic acid conjugations were found. The metabolites and their possible chemical structures, along with their fragmentation patterns are confirmed using MSMS (MS2) experiments in data-independent acquisition (DIA) mode. CONCLUSIONS: These findings serve as a vital tool for the rapid detection of methandienone, combating its illicit use in camel racing. Comprehensive screenings covering both the parent drug and its metabolites are recommended to improve detection accuracy and ensure regulatory compliance in sports doping. Future research should explore methandienone's metabolite profile in administered camel samples.


Assuntos
Anabolizantes , Camelus , Dopagem Esportivo , Fígado , Detecção do Abuso de Substâncias , Animais , Dopagem Esportivo/prevenção & controle , Detecção do Abuso de Substâncias/métodos , Fígado/metabolismo , Fígado/química , Anabolizantes/análise , Anabolizantes/metabolismo , Cromatografia Líquida/métodos , Espectrometria de Massas/métodos , Metandrostenolona/metabolismo , Metandrostenolona/análise , Metandrostenolona/química , Espectrometria de Massa com Cromatografia Líquida
2.
Int J Legal Med ; 135(4): 1449-1453, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33813613

RESUMO

A 32-year-old male went to the police to claim he just killed his girlfriend by inflicting several stabs with a kitchen knife. He was very nervous and particularly aggressive. About 90 min after the assault, a blood specimen was collected with natrium fluoride as preservative. The blood was free of alcohol, pharmaceuticals and drugs of abuse, but tested positive by LC-MS/MS for metandienone (32 ng/mL) and trenbolone (9 ng/mL). The perpetrator admitted regular consumption of anabolic steroids to enhance his muscular mass, as he was a professional security agent. To document long-term steroid abuse, a hair specimen was collected 3 weeks after the assault, which tested positive for both drugs. Segmental analyses revealed in the proximal 1.5 cm segment, corresponding to the period of the assault, the simultaneous presence of metandienone (11 pg/mg) and trenbolone (14 pg/mg), while only metandienone (3 pg/mg) was identified in the distal 1.5 cm segment. As aggressiveness and violence can be associated with abuse of anabolic steroids, the aetiology of this domestic crime was listed to be due impulsive behaviour in a context of antisocial lifestyle.


Assuntos
Anabolizantes/análise , Metandrostenolona/análise , Detecção do Abuso de Substâncias , Congêneres da Testosterona/análise , Acetato de Trembolona/análise , Adulto , Anabolizantes/efeitos adversos , Análise Química do Sangue , Análise do Cabelo , Homicídio/psicologia , Humanos , Masculino , Metandrostenolona/efeitos adversos , Transtornos Relacionados ao Uso de Substâncias/psicologia , Congêneres da Testosterona/efeitos adversos , Acetato de Trembolona/efeitos adversos , Violência/psicologia
3.
Environ Toxicol Chem ; 43(4): 915-925, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38085110

RESUMO

Steroid hormones (SHs) have received widespread attention in recent years. However, current studies of SHs have primarily focused on estrogenic substances, with androgen-related studies being quite limited. We optimized the solid-phase extraction (SPE) pretreatment method, as well as the enzymolysis conditions of five androgens (androstenedione, boldenone, methandienone, nandrolone, and testosterone), to simultaneously determine their concentrations in the effluent from wastewater treatment plants and surface water samples. Then we evaluated the ecological risks of the five androgens in the effluent and Pearl River basin of Guangzhou (PR China) using the risk quotient method. The recovery rates of the targets were 90% to 99% in water samples when digested with ß-glucosidase for 90 min before solid-phase extraction, extracted with a Poly-Sery HLB column, and washed with 15% methanol aqueous solution and 2% ammonia. The established instrument's limit of detection was between 0.02 and 0.39 µg/L, and the limit of quantification was between 0.05 and 1.29 µg/L. Androstenedione, boldenone, methandienone, nandrolone, and testosterone were detected in all samples from the 2018 and 2022 wastewater influent and the 2018 surface water, with concentrations of 3.06 × 101 ng/L to 1.33 × 103 ng/L, 1.03-8.15 × 102 ng/L, and 0.93 × 101 ng/L to 5.50 × 102 ng/L, respectively. The ecological risks of androgens in wastewater influent and surface water were medium to high and low to medium, respectively. Moreover, the biotoxicity of androgens was predicted by the Ecological Structure Activity Relationships model, with methandienone and androstenedione having the highest and lowest acute and chronic toxicities, respectively. These results suggest that the risk of environmental androgens should not be ignored and that further research should be carried out. Environ Toxicol Chem 2024;43:915-925. © 2023 SETAC.


Assuntos
Metandrostenolona , Nandrolona , Poluentes Químicos da Água , Androgênios , Águas Residuárias , Espectrometria de Massa com Cromatografia Líquida , Espectrometria de Massas em Tandem/métodos , Androstenodiona/análise , Metandrostenolona/análise , Esteroides , Testosterona , Água/química , Nandrolona/análise , Extração em Fase Sólida , Medição de Risco , Cromatografia Líquida de Alta Pressão/métodos , Poluentes Químicos da Água/análise
4.
Drug Test Anal ; 16(10): 1203-1218, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-38234065

RESUMO

Methandienone is an anabolic-androgenic steroid that is prohibited in equine sports due to its potential performance enhancing properties. Metabolism and detection of methandienone in equine urine have been investigated comprehensively in literature; however, there is a limited knowledge about its metabolites in equine plasma and no information about its detection in equine hair. Following a multi-dose oral administration of methandienone to two Thoroughbred horses, 17-epimethandienone, methyltestosterone, two mono-hydroxylated, two di-hydroxylated and three 17α-methylandrostanetriol metabolites were detected in plasma. The majority of these were present as free analytes, whilst the mono-hydroxylated metabolites and one isomer of 17α-methylandrostanetriol were partially conjugated. Estimated peak concentrations of methandienone were 6,000 and 11,100 pg/ml; meanwhile, they were 25.4 and 40.5 pg/ml for methyltestosterone. The most abundant analyte in the post-administration plasma samples of both horses was the mono-hydroxylated metabolite; however, the parent compound provided the longest detection (up to 96 h). Screening analysis of hair enabled the detection of methandienone in mane hair samples only, for up to 3 months. Its mono- and di-hydroxylated metabolites were detected with greater peak responses for up to 6 months post-administration in both mane and tail samples, showing that these metabolites could be better analytical targets for hair analysis when administered orally. A follow-up methodology with an extensive wash procedure confirmed the presence of methandienone and its metabolites in a number of post-administration hair samples. Final wash samples were also analysed to assess the degree of internal incorporation (via bloodstream) against possible external deposition (via sweat/sebum).


Assuntos
Anabolizantes , Dopagem Esportivo , Cabelo , Detecção do Abuso de Substâncias , Cavalos/metabolismo , Cavalos/urina , Animais , Administração Oral , Detecção do Abuso de Substâncias/métodos , Detecção do Abuso de Substâncias/veterinária , Cabelo/química , Cabelo/metabolismo , Anabolizantes/urina , Anabolizantes/metabolismo , Anabolizantes/análise , Anabolizantes/administração & dosagem , Anabolizantes/sangue , Metandrostenolona/urina , Metandrostenolona/metabolismo , Metandrostenolona/análise , Metandrostenolona/sangue , Masculino , Espectrometria de Massas em Tandem/métodos
5.
Forensic Sci Int ; 303: 109925, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31499423

RESUMO

A 34-year old male was found breathless and panting at home by his girlfriend three hours after a gym workout. Minutes later, he collapsed and died. Autopsy, histological and chemical analyses were conducted. The examination of the heart showed left ventricular hypertrophy, while the right coronary artery showed only a small vascular lumen (3 mm in diameter), due to its anatomical structure. In femoral blood concentrations of approx. 1 µg/L clenbuterol, approx. 56 µg/L stanozolol and approx. 8 µg/L metandienone, with trenbolone (

Assuntos
Anabolizantes/efeitos adversos , Clembuterol/efeitos adversos , Dopagem Esportivo , Metandrostenolona/efeitos adversos , Estanozolol/efeitos adversos , Adulto , Anabolizantes/análise , Androstanóis/urina , Clembuterol/análise , Clomifeno/urina , Vasos Coronários/patologia , Evolução Fatal , Insuficiência Cardíaca/induzido quimicamente , Humanos , Hipertrofia Ventricular Esquerda/patologia , Masculino , Metandrostenolona/análise , Estanozolol/análise , Testosterona/análogos & derivados , Testosterona/urina , Acetato de Trembolona/sangue , Acetato de Trembolona/urina
6.
Drug Test Anal ; 11(2): 336-340, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30548177

RESUMO

At present, anti-doping laboratories use androsterone, a major urinary steroid metabolite, to evaluate completeness of the derivatization step. This is typically done by calculating the ratio of mono-trimethylsilyl (TMS) androsterone to the total mono- and di-TMS androsterone. Certain samples may show an elevated percentage of mono-TMS androsterone indicating a failed derivatization step. In such cases, the laboratory would have to repeat the analysis or perform other remedial actions to ensure that completeness of derivatization is achieved. We have noticed that a poorly derivatized positive control sample spiked with various target analytes has a disproportionally low abundance of the di-TMS derivatives of boldenone and 18-nor-17ß-hydroxymethyl-17α-methylandrosta-1,4,13-trien-3-one (methandienone long-term metabolite). A follow-up investigation confirmed that 1,4-diene-3-one steroids are more likely to fail during the trimethylsilylation step. To better control derivatization efficiency, 13 C3 -boldenone (13C-BLD) was incorporated into our routine procedure as an additional internal standard. Analysis of a large number of urine samples has shown that derivatization of 13C-BLD could be grossly incomplete even in cases when mono-TMS androsterone is well below 1%. In other words, one or both of boldenone and the long-term metabolite of methandienone could remain undetected unless the laboratory has the means to recognize samples where derivatization of 1,4-diene-3-one steroids failed.


Assuntos
Isótopos de Carbono/análise , Controle de Qualidade , Detecção do Abuso de Substâncias/normas , Testosterona/análogos & derivados , Humanos , Metandrostenolona/análise , Metandrostenolona/urina , Detecção do Abuso de Substâncias/métodos , Testosterona/análise , Testosterona/química , Testosterona/urina
7.
Artigo em Inglês | MEDLINE | ID: mdl-29601260

RESUMO

Methandienone is a synthetic exogenous steroid which, like other anabolic steroids, is strictly regulated in many countries. In recent years, increasing numbers have been detected of illegal additions into dietary supplements of methandienone and other anabolic androgenic steroids (AAS). In this work, a competitive indirect enzyme-linked immunosorbent assay (ELISA) has been constructed for the detection of methandienone using an antiserum against methandienone. Under optimal experimental conditions, the ELISA achieved a limit of detection of 0.04 ± 0.01 µg.g-1. The obtained intra- and inter-day coefficients of variation were less than 8%. The developed ELISA was applied in the analysis of real dietary supplement samples. To minimise the effect of the sample matrix, the sample extracts were simply diluted before addition into the immunoassay. The achieved recovery values were around 100%. Results obtained from the ELISA correlated well, both in terms of accuracy and precision, with those obtained by UHPLC-MS/MS (reference method). The presented ELISA could be successfully applied for the simple screening of dietary supplements.


Assuntos
Suplementos Nutricionais/análise , Ensaio de Imunoadsorção Enzimática , Metandrostenolona/análise
8.
J Chromatogr B Analyt Technol Biomed Life Sci ; 836(1-2): 124-8, 2006 May 19.
Artigo em Inglês | MEDLINE | ID: mdl-16597518

RESUMO

A sensitive, specific and reproducible method for the quantitative determination of the anabolic metandienone in human hair has been developed. The preparation involved a decontamination step with methylene chloride. The hair sample (about 50 mg) was solubilised in 1 ml 1 M NaOH, 10 min at 95 degrees C, in presence of 2 ng of nandrolone-d(3) used as internal standard. The homogenate was neutralized and extracted using consecutively a solid-phase extraction (Isolute C(18) eluted with methanol) and a liquid-liquid extraction with pentane. The residue was derivatized by adding 5 microl MSTFA/NH(4)I/2-mercaptoethanol (250 microl; 5 mg; 15 microl) and 45 microl MSTFA, then incubated for 20 min at 60 degrees C. A 1 microl aliquot of derivatized extract was injected into the column (HP5-MS capillary column, 5% phenyl-95% methylsiloxane, 30 m x 0.32 mm i.d., 0.25 microm film thickness) of a Hewlett Packard (Palo Alto, CA, USA) gas chromatograph (6890 Series). Metandienone was identified using three transitions (its daughter ions at m/z 339 and 206 for the parent 444 and 191 for 206) using a Waters Quattro Micro MS-MS system. The transition m/z 444 to 206 has been used as quantification transition and the others as identification transitions. The assay was capable of detecting 2 pg/mg of metandienone when approximately 50 mg of hair material was processed. Linearity was observed for metandienone concentrations ranging from 2 to 500 pg/mg with a correlation coefficient of 0.9997. Intra-day and between-day precisions at 50 pg/mg were 13.4-16.5% and 22.0%, respectively, with an extraction recovery of 48%. The analysis of hair, cut into four segments, obtained from an athlete, revealed the presence of metandienone at the concentrations of 78, 7, 10 and 108 pg/mg in each segment of hair (0-1, 1-2, 2-3 and 3 cm to the tip).


Assuntos
Anabolizantes/análise , Dopagem Esportivo , Cromatografia Gasosa-Espectrometria de Massas/métodos , Cabelo/química , Metandrostenolona/análise , Humanos , Padrões de Referência , Reprodutibilidade dos Testes
9.
Drug Test Anal ; 8(3-4): 370-7, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26198290

RESUMO

The use of performance enhancing drugs is a widespread phenomenon in professional and leisure sports. A spectroscopic study was carried out on anabolic tablets labelled as 5 mg methandienone tablets provided by police departments. The analytical approach was based on a two-step methodology: a fast analysis of tablets using near infrared (NIR) spectroscopy to assess sample homogeneity based on their global composition, followed by Raman chemical imaging of one sample per NIR profile to obtain information on sample formulation. NIR spectroscopy assisted by a principal components analysis (PCA) enabled fast discrimination of different profiles based on the excipient formulation. Raman hyperspectral imaging and multivariate curve resolution - alternating least square (MCR-ALS) provided chemical images of the distribution of the active substance and excipients within tablets and facilitated identification of the active compounds. The combination of NIR spectroscopy and Raman chemical imaging highlighted dose-to-dose variations and succeeded in the discrimination of four different formulations out of eight similar samples of anabolic tablets. Some samples contained either methandienone or methyltestosterone whereas one sample did not contain an active substance. Other ingredients were sucrose, lactose, starch or talc. Both techniques were fast and non-destructive and therefore can be carried out as exploratory methods prior to destructive screening methods. Copyright © 2015 John Wiley & Sons, Ltd.


Assuntos
Anabolizantes/análise , Metandrostenolona/análise , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Análise Espectral Raman/métodos , Excipientes/análise , Excipientes/química , Análise dos Mínimos Quadrados , Análise de Componente Principal , Comprimidos , Tecnologia Farmacêutica/métodos
10.
J Chromatogr Sci ; 43(1): 2-6, 2005 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-15808000

RESUMO

A sensitive and selective method for the screening of anabolizing agents in aqueous nutritional supplements is described and validated. A total of 28 different anabolizing agents are screened for, including testosterone and prohormones, nandrolone and prohormones, stanozolol, and metandienone. The different analytes are extracted from the aqueous nutritional supplements by liquid-liquid extraction with a mixture of pentane and freshly distilled diethylether (1:1) after the supplements have been made alkaline with a NaHCO3-K2CO3 (2:1) buffer. The anabolizing agents are derivatized with a mixture of MSTFA-NH4I-ethanethiol (320:1:2) as routinely used for the screening of anabolic steroids extracted from urine. The derivatives are analyzed by gas chromatography (GC)-mass spectrometry (MS) in the selective ion monitoring mode. The limits of detection range from 1 to 10 ng/mL. One aqueous nutritional supplement (creatine serum) was analyzed with this screening method and was found to contain dehydroepiandrosterone (DHEA) at very low concentrations. The presence of DHEA could be confirmed with GC-MS-MS. Results of the application of this method and a similar method for solid nutritional supplements previously described are given.


Assuntos
Anabolizantes/análise , Suplementos Nutricionais/análise , Cromatografia Gasosa-Espectrometria de Massas/métodos , Desidroepiandrosterona/análise , Dopagem Esportivo/prevenção & controle , Indicadores e Reagentes , Metandrostenolona/análise , Nandrolona/análise , Fenômenos Fisiológicos da Nutrição , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Estanozolol/análise , Testosterona/análise , Água
11.
Artigo em Inglês | MEDLINE | ID: mdl-25719897

RESUMO

A sensitive method for the identification and quantification of anabolic steroids and clenbuterol at trace levels in dietary supplements by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) in atmospheric pressure ionisation (APCI) mode using a single-stage Orbitrap analyser operating at a resolution power of 100 000 full width at half maximum (FWHM) was developed and validated. A total of 1 g of dietary supplement was added with testosterone-d3 as internal standard, dissolved in methanol, evaporated to dryness, diluted in sodium hydroxide solution and extracted with a mixture of pentane/ethyl ether 9:1. The extract was directly injected into the LC-HRMS system. The method was fully validated. Limits of detection (LODs) obtained for anabolic androgenic steroids (AASs) varied from 1 to 25 ng g(-1) and the limit of quantitation (LOQ) was 50 ng g(-1) for all analytes. The calibration was linear for all compounds in the range from the LOQ to 2000 ng g(-1), with correlation coefficients always higher than 0.99. Accuracy (intended as %E) and repeatability (%CV) were always lower than 15%. Good values of matrix effect and recovery were achieved. The ease of the sample preparation together with a fast run time of only 16 min permitted rapid identification of the analytes. The method was applied to the analysis of 30 dietary supplements in order to check for the presence of anabolic agents not labelled as being present in these supplements. Many AASs were often detected in the same sample: indeed, androstenedione was detected in nine supplements, 5-androsten-3ß-ol-17-one (DHEA) in 12, methandienone in three, stanozolol in one, testosterone in seven and testosterone esters in four of them. A retrospective analysis of suspected compounds not included at the beginning of the method development was also possible by means of the full acquisition spectra obtained with the HRMS technique.


Assuntos
Anabolizantes/análise , Cromatografia Líquida , Suplementos Nutricionais/análise , Espectrometria de Massas , Desidroepiandrosterona/análise , Limite de Detecção , Metandrostenolona/análise , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Estanozolol/análise , Testosterona/análogos & derivados , Testosterona/análise , Propionato de Testosterona/análogos & derivados , Propionato de Testosterona/análise
12.
J Pharm Sci ; 64(3): 441-3, 1975 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-1151630

RESUMO

A high-speed liquid chromatographic system is described, which can be used for the simultaneous identification of the anabolic steroid methandrostenolone and its impurities and the quantitation of each of these compounds. Separation is effected by adsorption chromatography on a slurry-packed microparticulate silica gel column.


Assuntos
Metandrostenolona/análise , Esteroides/análise , Cromatografia , Métodos , Comprimidos/análise
13.
J Chromatogr Sci ; 28(4): 162-6, 1990 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-2079544

RESUMO

The liquid chromatographic properties of various 17-hydroxy anabolic steroids are examined under reversed-phase conditions. These anabolic steroids are now listed as controlled drugs in many states due to their abuse potential in athletics, body building, and other areas. These nonesterified steroids are separated on a C18 stationary phase with a 70% methanol in water mobile phase. In a few cases, two compounds display very similar retention properties. However, dual-wavelength detection at 254 and 280 nm allows for their differentiation. Reversed-phase retention parallels steroid lipophilicity based on hydroxyl and methyl group substituents. Also, those steroids containing a dienone substructure are more polar than steroids containing an enone moiety.


Assuntos
Anabolizantes/análise , Cromatografia Líquida/métodos , Hidroxiesteroides/análise , Espectrometria de Massas/métodos , Anabolizantes/química , Danazol/análise , Danazol/química , Fluoximesterona/análise , Fluoximesterona/química , Humanos , Hidroxiesteroides/química , Metandrostenolona/análise , Metandrostenolona/química , Metiltestosterona/análise , Metiltestosterona/química , Nandrolona/análise , Nandrolona/química , Testosterona/análogos & derivados , Testosterona/análise , Testosterona/química
14.
Vopr Med Khim ; 29(3): 125-7, 1983.
Artigo em Russo | MEDLINE | ID: mdl-6880114

RESUMO

Various conditions of radioimmunoassay (RIA) technique for estimation of 19-nortestosterone and methandrostenolone were studied. Dilution of antiserum, required for 50% binding of the labelled steroids, depended primarily on the RIA procedure used. When 19-nortestosterone was estimated by means of these two RIA procedures, the final dilution of antiserum was equal to 1:24,000 and 1:475,000, respectively. Characteristics of standard curves, sensitivity and accuracy were similar in these RIA procedures, but the rate of cross-reactions between antiserum and the steroids was different. Both RIA procedures indicated similar values of methandroctenolone and 19-nortestosterone content excreted with urine after oral administration of the steroids.


Assuntos
Metandrostenolona/análise , Nandrolona/análise , Reações Cruzadas , Humanos , Metandrostenolona/urina , Nandrolona/urina , Radioimunoensaio/métodos
15.
J Chromatogr B Analyt Technol Biomed Life Sci ; 879(22): 2149-54, 2011 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-21703946

RESUMO

Methandrostenolone (MA) is a steroid used as veterinary medicine on stockbreeding to promote animal growth. The use of MA has been strictly regulated because of its harmful effect on consumers. This paper describes the production of polyclonal antibody (pAb) against MA, the preparation of immunoaffinity column (IAC) and its potential application to the selective extraction of MA residues from animal tissue and feed samples. The produced pAb exhibited good sensitivity to MA with an IC(50) value of 5.6 ng/mL. The cross-reactivity values of the antibody with MA structurally related compounds of testosterone propionate (TP) and trenbolone (TR) were lower than 0.6%. By coupling the produced antibody with CNBr-activated Sepharose 4B, an IAC was prepared. 2% methanol and 80% methanol were selected as loading and eluting solution by optimization. The maximum capacity of the column for MA was approximately 334 ng/mL gel. The average recovery of 20, 40 and 60 ng/mL MA standard solutions from IACs was 97.9% with the relative standard deviation (RSD) among columns of 6.7%. After 3 times of repeated usage, the column capacity and recovery rate still remained 82.0% and 92.6% respectively. The IACs were then challenged with MA-fortified animal tissue and feed samples, recoveries of MA were found to be in the range of 83.5-99.7%.


Assuntos
Resíduos de Drogas/isolamento & purificação , Técnicas de Imunoadsorção , Metandrostenolona/isolamento & purificação , Ração Animal/análise , Animais , Anticorpos/metabolismo , Cromatografia Líquida de Alta Pressão/métodos , Resíduos de Drogas/análise , Resíduos de Drogas/metabolismo , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Feminino , Carne/análise , Metandrostenolona/análise , Metandrostenolona/metabolismo , Metanol , Coelhos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Espectrofotometria Ultravioleta , Suínos
20.
Biomed Chromatogr ; 21(2): 164-8, 2007 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-17146762

RESUMO

In numerous studies it has been demonstrated that several nutritional supplements contain prohormones not declared on the label. In the current study two products (effervescent tablets) containing high amounts of the 17-methylated anabolic androgenic steroids metandienone (product 1: 16.8 mg/tablet) and stanozolol (product 2: 14.5 mg/tablet) were identified. Additionally in both products norandrostenedione was detected, in product 2 with minor amounts of several other steroids. The substances identified can cause enormous health risks. In addition, the use of the analyzed tablets can lead to positive doping results for metabolites of the respective steroids in sports. This study again shows the insufficient surveillance of the production and trade of dietary supplements. Consumers should be aware of the enormous health and doping risks connected with the use of such products. For GC-MS identification of the analytes the trimethylsilyl derivatives of the steroids and the mixed N-t-butyldimethylsilyl,O-trimethylsilyl derivatives were used. The quantitation of metandienone, norandrostenedione, and stanozolol was performed using HPLC-DAD.


Assuntos
Anabolizantes/análise , Androgênios/análise , Suplementos Nutricionais/análise , Metandrostenolona/análise , Estanozolol/análise , Cromatografia Líquida de Alta Pressão/métodos , Suplementos Nutricionais/normas , Dopagem Esportivo/prevenção & controle , Cromatografia Gasosa-Espectrometria de Massas/métodos , Compostos de Trimetilsilil/análise , Compostos de Trimetilsilil/química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA