RESUMO
BACKGROUND & OBJECTIVES: Various conventional methods such as gaseous, vapour and misting systems, fogging, manual spray and wipe techniques employing a number of chemical agents are used for decontamination of enclosed spaces. Among all these methods, use of aerosolized formaldehyde is the most preferred method due to cost-effectiveness and practical aspects. However, being extremely corrosive in nature generating very irritating fumes and difficulty in maintaining a high level of gas concentration, many laboratories prefer the vaporization of hydrogen peroxide (H 2 O 2 ) as an alternative. We present here the results of using H 2 O 2 vapour in combination with plasma irradiation for quick decontamination of closed chambers. METHODS: The present study describes a decontamination method, using plasma irradiation in combination with H 2 O 2 (5%). Effect of plasma irradiation and H 2 O 2 on the viability of bacterial spores (Bacillus subtilis), Chikungunya and Kyasanur Forest Disease viruses was assessed. RESULTS: Data suggest that with the combination of H 2 O 2 vapour and plasma irradiation, within short time (three minutes), decontamination of surfaces and space volume could be achieved. Although it showed damage of spores present on the strips, it did not show any penetration power. INTERPRETATION & CONCLUSIONS: The results were encouraging, and this method was found to be efficient for achieving surface sterilization in a short time. This application may be useful in laboratories and industries particularly, those working on clean facility concept following good laboratory and manufacturing practices.
Assuntos
Descontaminação/métodos , Contaminação de Equipamentos/prevenção & controle , Peróxido de Hidrogênio/farmacologia , Gases em Plasma/farmacologia , Bacillus subtilis/efeitos dos fármacos , Bacillus subtilis/crescimento & desenvolvimento , Febre de Chikungunya/prevenção & controle , Febre de Chikungunya/virologia , Vírus Chikungunya/efeitos dos fármacos , Vírus Chikungunya/crescimento & desenvolvimento , Vírus Chikungunya/efeitos da radiação , Vírus da Encefalite Transmitidos por Carrapatos/efeitos dos fármacos , Vírus da Encefalite Transmitidos por Carrapatos/efeitos da radiação , Humanos , Doença da Floresta de Kyasanur/prevenção & controle , Doença da Floresta de Kyasanur/virologia , Esporos Bacterianos/efeitos dos fármacos , Esporos Bacterianos/crescimento & desenvolvimento , Esporos Bacterianos/efeitos da radiação , Esterilização , VolatilizaçãoRESUMO
Effects of low-intensive laser exposure in the 630 to 890 nm waveband on the course of experimental tick-borne encephalitis were studied in vitro and in vivo. Virus-static effect of the exposure was revealed: titers of the exposed virus dropped, infective activity decreased, and interferon production was inhibited. The exposure had no apparent effect on the virus which penetrated into the cells. In addition to its manifest prophylactic effect, low-intensive laser exposure is capable of activating the infectious process during the acute period of tick-borne encephalitis by causing more frequent and sooner death of animals. Effects of interferon inducers during the acute period of viral infections are discussed.