Steroid monooxygenase of Rhodococcus rhodochrous: sequencing of the genomic DNA, and hyperexpression, purification, and characterization of the recombinant enzyme.

Morii, S; Sawamoto, S; Yamauchi, Y; Miyamoto, M; Iwami, M; Itagaki, E

Morii, S; Sawamoto, S; Yamauchi, Y; Miyamoto, M; Iwami, M; Itagaki, E.

Affiliation

Morii S; Department of Chemistry Faculty of Science, Kanazawa University, Kakuma-machi, Kanazawa, 920-1192, Japan.

J Biochem ; 126(3): 624-31, 1999 Sep.

Article in En | MEDLINE | ID: mdl-10467180

Subject(s)

Rhodococcus/enzymology; Steroid Hydroxylases/genetics; Amino Acid Sequence; Base Sequence; Catalysis; Chromatography, DEAE-Cellulose; Cloning, Molecular; DNA, Bacterial; Electrophoresis, Polyacrylamide Gel; Molecular Sequence Data; Open Reading Frames; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Sequence Homology, Amino Acid; Steroid Hydroxylases/isolation & purification; Steroid Hydroxylases/metabolism

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Collection: 01-internacional Database: MEDLINE Main subject: Steroid Hydroxylases / Rhodococcus Language: En Journal: J Biochem Year: 1999 Type: Article Affiliation country: Japan

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